SRR2935369.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	709844
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11596	1.6335983680921442	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	5876	0.8277875138762884	RNA PCR Primer, Index 29 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	3781	0.5326522447185579	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	3750	0.5282850879911642	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	3675	0.517719386231341	RNA PCR Primer, Index 29 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	2772	0.39050833704306864	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	2083	0.29344475687615873	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG	2059	0.29006373231301524	RNA PCR Primer, Index 29 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	2030	0.28597832763255027	RNA PCR Primer, Index 29 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	2017	0.2841469393275142	Illumina PCR Primer Index 8 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	1684	0.23723522351389884	TruSeq Adapter, Index 12 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1431	0.2015935895774283	RNA PCR Primer, Index 29 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	1246	0.17553152523653084	RNA PCR Primer, Index 29 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	1230	0.17327750886110188	RNA PCR Primer, Index 29 (96% over 26bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	917	0.1291833135167727	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	801	0.11284169479491268	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	782	0.11016505034909078	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	20	7.0314604E-4	45.000004	1
ACTACGC	20	7.0314604E-4	45.000004	39
ACGTAGT	20	7.0314604E-4	45.000004	33
CGACGGT	65	0.0	44.999996	28
CGGTCTA	60	0.0	44.999996	31
CGTTTTT	6340	0.0	44.00631	1
CGTTTTC	300	0.0	42.000004	1
CGTTTCT	460	0.0	40.108692	1
CGGCGAA	40	3.4557343E-7	39.375004	31
ATACGCG	40	3.4557343E-7	39.375004	1
TTGTGCG	35	6.2449126E-6	38.57143	1
CGTTGAT	30	1.1394822E-4	37.499996	25
CAACCGG	30	1.1394822E-4	37.499996	2
CTTGCGG	60	1.546141E-10	37.499996	2
ACGTGCG	30	1.1394822E-4	37.499996	1
GACGCGC	30	1.1394822E-4	37.499996	16
TCGAGCG	30	1.1394822E-4	37.499996	1
CGCAATC	30	1.1394822E-4	37.499996	20
TATACGG	30	1.1394822E-4	37.499996	2
TTCGACG	30	1.1394822E-4	37.499996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content