##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	617825
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93126856310444	33.0	31.0	34.0	30.0	34.0
2	31.98526929146603	33.0	31.0	34.0	30.0	34.0
3	32.049891150406665	33.0	31.0	34.0	30.0	34.0
4	35.77227208351879	37.0	35.0	37.0	35.0	37.0
5	35.79247359689232	37.0	35.0	37.0	35.0	37.0
6	35.741413830777326	37.0	35.0	37.0	35.0	37.0
7	36.022274916036096	37.0	35.0	37.0	35.0	37.0
8	35.863560069599	37.0	35.0	37.0	35.0	37.0
9	37.68237931453081	39.0	38.0	39.0	35.0	39.0
10	37.0711981548173	39.0	37.0	39.0	33.0	39.0
11	37.03677416744224	39.0	37.0	39.0	33.0	39.0
12	37.0339594545381	39.0	37.0	39.0	33.0	39.0
13	36.97015174199814	39.0	37.0	39.0	33.0	39.0
14	37.961663901590256	40.0	37.0	41.0	33.0	41.0
15	38.105421438109495	40.0	37.0	41.0	33.0	41.0
16	38.07366810990167	40.0	37.0	41.0	33.0	41.0
17	38.082287055395945	40.0	37.0	41.0	33.0	41.0
18	37.93074414275887	39.0	37.0	41.0	33.0	41.0
19	37.72837292113463	39.0	37.0	41.0	33.0	41.0
20	37.66434993727997	39.0	35.0	41.0	33.0	41.0
21	37.60966940476672	39.0	35.0	41.0	33.0	41.0
22	37.730289321409785	39.0	35.0	41.0	33.0	41.0
23	37.74384493991017	39.0	35.0	41.0	34.0	41.0
24	37.70343058309392	39.0	35.0	41.0	33.0	41.0
25	37.46918140250071	39.0	35.0	41.0	33.0	41.0
26	37.53213288552584	39.0	35.0	41.0	33.0	41.0
27	37.550193015821634	39.0	35.0	41.0	33.0	41.0
28	37.45468862541982	39.0	35.0	41.0	33.0	41.0
29	37.41417715372476	39.0	35.0	41.0	33.0	41.0
30	37.23652814308259	39.0	35.0	41.0	33.0	41.0
31	37.14489216201999	39.0	35.0	41.0	32.0	41.0
32	36.984617003196696	39.0	35.0	41.0	32.0	41.0
33	36.72621211508113	39.0	35.0	41.0	31.0	41.0
34	36.47093270748189	39.0	35.0	41.0	30.0	41.0
35	36.32090316837292	39.0	35.0	41.0	30.0	41.0
36	36.17504795047141	39.0	35.0	41.0	29.0	41.0
37	36.073687532877436	39.0	35.0	41.0	29.0	41.0
38	35.966259053939226	39.0	35.0	41.0	29.0	41.0
39	35.88409015497916	39.0	35.0	41.0	28.0	41.0
40	35.774678913931936	39.0	35.0	41.0	27.0	41.0
41	35.621404119289444	39.0	35.0	41.0	26.0	41.0
42	35.631400477481485	39.0	35.0	41.0	26.0	41.0
43	35.59116578319103	39.0	35.0	41.0	26.0	41.0
44	35.38480799579169	39.0	35.0	41.0	25.0	41.0
45	35.389958321531175	39.0	35.0	41.0	25.0	41.0
46	35.340483146522075	38.0	35.0	41.0	25.0	41.0
47	35.299576740986524	38.0	35.0	40.0	25.0	41.0
48	35.21383077732368	38.0	35.0	40.0	24.0	41.0
49	35.15560878889653	38.0	35.0	40.0	24.0	41.0
50	35.0535815158014	38.0	34.0	40.0	24.0	41.0
51	33.93130093473071	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	5.0
11	9.0
12	14.0
13	13.0
14	23.0
15	34.0
16	54.0
17	120.0
18	214.0
19	389.0
20	681.0
21	1083.0
22	1638.0
23	2264.0
24	3621.0
25	6041.0
26	9395.0
27	11066.0
28	10961.0
29	10481.0
30	10978.0
31	12990.0
32	16251.0
33	22957.0
34	38470.0
35	42478.0
36	47082.0
37	67628.0
38	123409.0
39	177394.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.748472463885406	20.281147574151255	22.999716748270142	16.9706632136932
2	36.61150002023227	24.332780318051228	23.46182171326832	15.593897948448184
3	29.730587140371462	23.678226034880428	31.260308339740217	15.33087848500789
4	26.002832517298586	24.14599603447578	31.998057702423825	17.853113745801803
5	24.227734390806457	27.80577024238255	29.990531299316146	17.97596406749484
6	24.58689758426739	32.320155383806096	29.303443531744428	13.789503500182091
7	75.17500910451989	5.526969611135839	15.034192530247239	4.263828754097034
8	77.01549791607655	5.443288957228988	13.619228746004127	3.921984380690325
9	70.76469874155303	7.101687371019301	15.553757131873914	6.579856755553758
10	37.01695463925869	28.24149233197103	21.733500586735726	13.008052442034558
11	28.249423380407073	23.624165419010236	31.175332820782586	16.951078379800105
12	25.206004936673004	21.941488285517742	34.41055315016388	18.44195362764537
13	23.785699834095414	21.92708291182778	36.437016954639255	17.850200299437542
14	19.834580989762472	25.497511431230524	35.27762715979444	19.390280419212562
15	19.020758305345364	24.364990086189454	37.68381014041193	18.930441468053253
16	23.236838910694775	24.685954760652287	33.37110023064784	18.706106098005097
17	23.918423501800675	24.219479626107717	32.268037065512075	19.594059806579534
18	23.78456682717598	23.966819083073688	33.25828511309837	18.990328976651966
19	22.698822482094442	25.851980738882368	31.781977097074414	19.667219681948772
20	23.13308784850079	26.616274835107028	31.913244041597537	18.337393274794643
21	22.64816088698256	26.35438837858617	33.465949095617695	17.53150163881358
22	22.224416299113827	22.606077772832112	34.538906648322744	20.630599279731314
23	20.897665196455307	25.116659248168983	33.78011572856391	20.2055598268118
24	21.055962448913526	25.32869340023469	33.34261319953061	20.272730951321165
25	21.804556306397444	26.451988831788935	31.478007526403108	20.265447335410514
26	20.828551774369767	25.527455185529885	31.73050621130579	21.91348682879456
27	20.58236555658965	25.38469631368106	33.399263545502365	20.633674584226924
28	19.472018775543237	26.028244243920206	33.18480152146643	21.314935459070124
29	20.676405130902765	26.545866547970704	32.18824100675758	20.589487314368956
30	21.45607574960547	25.49427426860357	33.450410715008296	19.599239266782664
31	22.104964998179096	26.333023105248248	31.56541091733096	19.996600979241695
32	23.77323675798163	26.682960385222355	30.02403593250516	19.51976692429086
33	23.203981710031158	25.827702019180187	29.686238012382148	21.282078258406507
34	21.313640594019343	27.687451948367258	30.719702181038322	20.279205276575084
35	22.470278800631245	27.393841298102213	29.835633067616236	20.300246833650306
36	23.34997774450694	28.630761137862663	28.058754501679278	19.960506615951118
37	22.824262533889048	28.3539837332578	29.18075506818274	19.640998664670416
38	23.05054020151337	27.53789503500182	28.4983611864201	20.913203577064703
39	23.377008052442033	26.458786873305545	28.309796463399827	21.854408610852587
40	24.30672115890422	25.41350706106098	29.294217618257594	20.985554161777202
41	22.072431513778174	26.502974143163517	29.366568202970093	22.058026140088213
42	22.138307773236757	26.26002508801036	30.342896451260472	21.25877068749241
43	22.560433779792014	25.50414761461579	30.09201634767127	21.843402257920932
44	22.058835430744953	25.908307368591434	29.74240278395986	22.29045441670376
45	21.875450167927813	25.295188767045683	29.27252862865698	23.556832436369522
46	22.259863229879013	26.2605106624044	28.857362521749685	22.622263585966902
47	20.451907902723264	26.39469105329179	31.42249018735079	21.73091085663416
48	21.319467486747865	26.729899243313238	30.36895561040748	21.58167765953142
49	22.12131266944523	24.94460405454619	31.086148990409907	21.847934285598672
50	20.610852587706873	25.212964836320968	31.340751831020107	22.83543074495205
51	20.66167604095011	25.295026908914338	29.879658479342858	24.1636385707927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	386.0
1	590.0
2	794.0
3	7858.0
4	14922.0
5	9799.0
6	4676.0
7	4573.0
8	4470.0
9	4456.5
10	4443.0
11	4296.0
12	4149.0
13	4046.5
14	3944.0
15	3722.5
16	3501.0
17	3388.5
18	3276.0
19	3133.5
20	2991.0
21	3035.0
22	3079.0
23	3362.0
24	3645.0
25	4197.5
26	5596.5
27	6443.0
28	7264.5
29	8086.0
30	9439.0
31	10792.0
32	12368.5
33	13945.0
34	15753.0
35	17561.0
36	19484.5
37	21408.0
38	22964.0
39	24520.0
40	26540.0
41	28560.0
42	30633.0
43	32706.0
44	35306.5
45	37907.0
46	45572.0
47	53237.0
48	53398.5
49	53560.0
50	52324.0
51	51088.0
52	45086.0
53	39084.0
54	35553.5
55	32023.0
56	30021.0
57	28019.0
58	26662.0
59	25305.0
60	23176.0
61	21047.0
62	18550.5
63	16054.0
64	14171.0
65	12288.0
66	10522.5
67	8757.0
68	7319.0
69	5881.0
70	4967.5
71	4054.0
72	3384.5
73	2715.0
74	2237.0
75	1325.5
76	892.0
77	716.0
78	540.0
79	455.5
80	371.0
81	229.5
82	88.0
83	67.0
84	46.0
85	29.5
86	13.0
87	20.0
88	27.0
89	17.5
90	8.0
91	6.5
92	5.0
93	5.0
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	617825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.306010438511954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50362781514154	25.951198509098845
2	8.256875969583731	5.8303469834324355
3	3.396861402265773	3.5978887237972117
4	2.166263238175587	3.0592844999836792
5	1.568064609320718	2.7681052732469222
6	1.285201686062617	2.722520648623195
7	1.1032146070976747	2.726507450387655
8	0.967820940295527	2.733591697654746
9	0.8345910442327886	2.651947210761428
>10	6.844951844030627	41.76252418522898
>50	0.04360791240950731	1.0018883748342962
>100	0.022492502187467206	1.4248790991083236
>500	0.0036722452550966866	0.9018940157572588
>1k	0.002295153284435429	1.3477359631693377
>5k	4.590306568870858E-4	1.5196873649156903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9377	1.5177436976490106	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	2419	0.3915348197305062	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	1884	0.3049407194593938	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	1847	0.2989519685995225	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT	1156	0.18710799983814186	No Hit
CGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTG	1010	0.16347671266135233	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	936	0.15149921094160967	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	804	0.13013393760369038	No Hit
CGTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	787	0.1273823493707765	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	684	0.11071096184194554	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	658	0.10650265042690082	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	637	0.10310362966859549	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09064055355480921	0.0
2	0.0	0.0	0.0	0.4172702626148181	0.0
3	0.0	0.0	0.0	0.7332173350058674	0.0
4	0.0	0.0	0.0	1.1610083761582972	0.0
5	0.0	0.0	0.0	2.2708695828106666	0.0
6	0.0	0.0	0.0	3.198154817302634	0.0
7	0.0	0.0	0.0	3.910654311495974	0.0
8	0.0	0.0	0.0	4.81366082628576	0.0
9	0.0	0.0	0.0	5.310241573261036	0.0
10	0.0	0.0	0.0	6.350180067171125	0.0
11	1.618581313478736E-4	0.0	0.0	7.109780277586696	0.0
12	1.618581313478736E-4	0.0	0.0	7.986242058835431	0.0
13	1.618581313478736E-4	0.0	0.0	8.302998421883219	0.0
14	1.618581313478736E-4	0.0	0.0	8.491401286772144	0.0
15	3.237162626957472E-4	0.0	0.0	8.702464290049772	0.0
16	3.237162626957472E-4	0.0	0.0	9.02148666693643	0.0
17	3.237162626957472E-4	0.0	0.0	9.419010237526807	0.0
18	3.237162626957472E-4	0.0	0.0	9.868975842673896	0.0
19	3.237162626957472E-4	0.0	0.0	10.18314247562012	0.0
20	3.237162626957472E-4	0.0	0.0	10.472221098207422	0.0
21	3.237162626957472E-4	0.0	0.0	10.789624893780601	0.0
22	3.237162626957472E-4	0.0	0.0	11.155909845020838	0.0
23	3.237162626957472E-4	0.0	0.0	11.494355197669243	0.0
24	3.237162626957472E-4	0.0	0.0	11.797839193946507	0.0
25	3.237162626957472E-4	0.0	0.0	12.062800954962976	0.0
26	3.237162626957472E-4	0.0	0.0	12.33941650143649	0.0
27	3.237162626957472E-4	0.0	0.0	12.637235463116578	0.0
28	4.8557439404362077E-4	0.0	0.0	12.914660300246833	0.0
29	4.8557439404362077E-4	0.0	0.0	13.24307044875167	0.0
30	4.8557439404362077E-4	0.0	0.0	13.675069801319143	0.0
31	6.474325253914944E-4	0.0	0.0	13.984704406587626	0.0
32	8.092906567393679E-4	0.0	0.0	14.316189859588071	0.0
33	9.711487880872415E-4	0.0	0.0	14.632460648241816	0.0
34	9.711487880872415E-4	0.0	0.0	14.968154412657306	0.0
35	9.711487880872415E-4	0.0	0.0	15.315178246267147	0.0
36	0.001133006919435115	0.0	0.0	15.637437785780763	0.0
37	0.001133006919435115	0.0	0.0	15.975559422166471	0.0
38	0.001133006919435115	0.0	0.0	16.355764172702624	0.0
39	0.0012948650507829888	0.0	0.0	16.914660300246833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	35	1.2107012E-7	45.000004	1
TCACGAC	20	7.03075E-4	45.0	25
CTTCGTC	20	7.03075E-4	45.0	41
GTATGCG	20	7.03075E-4	45.0	1
CGCCCCG	20	7.03075E-4	45.0	25
TACGTGG	30	2.1638662E-6	44.999996	2
CGTTTTT	4530	0.0	43.013245	1
TACGGCT	225	0.0	41.0	7
CTACGAA	45	1.925764E-8	40.0	11
TGTAACG	45	1.925764E-8	40.0	1
ACAACGA	145	0.0	38.793106	13
TTGTACG	35	6.2435975E-6	38.57143	1
TTAACGG	35	6.2435975E-6	38.57143	2
ACGACCA	35	6.2435975E-6	38.57143	28
CACAACG	150	0.0	37.500004	12
CGAAACT	30	1.13931106E-4	37.499996	33
TTAAGCG	30	1.13931106E-4	37.499996	1
TACGAAG	30	1.13931106E-4	37.499996	1
ACGGAAT	30	1.13931106E-4	37.499996	5
TAGTACG	30	1.13931106E-4	37.499996	1
>>END_MODULE