FastQCFastQC Report
Sat 14 Jan 2017
SRR2935364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746606
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC158162.11838640460966No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116821.5646807017355875No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG101681.361896368365644No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC92381.237332676137079No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT42700.5719214686193254No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT35140.47066324138836285No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC31080.41628382306062367No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT20580.27564739635095353No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT19560.26198557204201417No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC17490.23426010506210773No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTG17350.2323849527059788No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC15700.2102849427944592No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT14700.19689099739353824No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA13430.1798806867343686No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT11760.15751279791483058No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10890.14586006541602933No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT9040.12108126642432555No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC8680.116259446079994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAG207.031697E-445.01
CGGTCTA351.2111559E-745.031
CGATCCA207.031697E-445.034
CGACTGG302.1644755E-644.9999962
CGTTTTT61200.043.7132381
CGTTAGG700.041.7857132
CGTAAGG700.041.7857132
CGACGAA650.041.5384619
CGATGAA7250.040.03448519
TACGGCT11300.040.0221257
TATAGCG403.4560435E-739.3751
CCGATGA7150.039.33566318
ACGGCTG11700.039.0384648
ATCGAAT356.245349E-638.57142643
GTTTTTT72900.037.7160532
GAATCTG22350.037.348991
GATGAAT8350.036.91617220
CCACCGA11550.036.62337515
AATCTGT22900.036.0589522
TTGATCG250.002106636536.01
Produced by FastQC (version 0.11.5)