##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746606
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.806093173641788	33.0	31.0	34.0	30.0	34.0
2	31.867158849513665	33.0	31.0	34.0	30.0	34.0
3	31.93501525570381	33.0	31.0	34.0	30.0	34.0
4	35.6672635901667	37.0	35.0	37.0	33.0	37.0
5	35.66928741531678	37.0	35.0	37.0	33.0	37.0
6	35.59339598128062	37.0	35.0	37.0	33.0	37.0
7	35.84656164027613	37.0	35.0	37.0	35.0	37.0
8	35.629542221734084	37.0	35.0	37.0	33.0	37.0
9	37.4061204972904	39.0	37.0	39.0	34.0	39.0
10	36.90153708917421	39.0	37.0	39.0	32.0	39.0
11	36.89079380556813	39.0	37.0	39.0	33.0	39.0
12	36.86113827105595	39.0	35.0	39.0	33.0	39.0
13	36.777675239684655	39.0	35.0	39.0	33.0	39.0
14	37.7303879154467	40.0	37.0	41.0	33.0	41.0
15	37.798355491383674	40.0	37.0	41.0	32.0	41.0
16	37.83497185932071	40.0	36.0	41.0	33.0	41.0
17	37.76926652076195	40.0	36.0	41.0	33.0	41.0
18	37.60182345172688	39.0	36.0	41.0	33.0	41.0
19	37.39818860282398	39.0	36.0	41.0	32.0	41.0
20	37.23188535854253	39.0	35.0	41.0	32.0	41.0
21	37.268481635561464	39.0	35.0	41.0	32.0	41.0
22	37.404253381301515	39.0	35.0	41.0	33.0	41.0
23	37.417195950742425	39.0	35.0	41.0	33.0	41.0
24	37.3900745507001	39.0	35.0	41.0	33.0	41.0
25	37.194995754119304	39.0	35.0	41.0	33.0	41.0
26	37.20623193491614	39.0	35.0	41.0	33.0	41.0
27	37.20339509728023	39.0	35.0	41.0	33.0	41.0
28	37.03373265149222	39.0	35.0	41.0	32.0	41.0
29	36.99802305365883	39.0	35.0	41.0	32.0	41.0
30	36.80470020332009	39.0	35.0	41.0	31.0	41.0
31	36.700467180815586	39.0	35.0	41.0	31.0	41.0
32	36.42181284372212	39.0	35.0	41.0	30.0	41.0
33	36.15631002161783	39.0	35.0	41.0	30.0	41.0
34	35.859580555205824	39.0	35.0	41.0	28.0	41.0
35	35.621843381917635	39.0	35.0	41.0	26.0	41.0
36	35.52999841951444	39.0	35.0	41.0	25.0	41.0
37	35.5040088078585	39.0	35.0	41.0	25.0	41.0
38	35.37541621685333	39.0	35.0	41.0	24.0	41.0
39	35.22963651510971	39.0	35.0	41.0	23.0	41.0
40	35.08440060754936	38.0	35.0	40.0	23.0	41.0
41	34.88464598462911	38.0	34.0	40.0	23.0	41.0
42	34.93450494638404	38.0	35.0	40.0	23.0	41.0
43	34.936212674422656	38.0	35.0	40.0	23.0	41.0
44	34.90534498785169	38.0	34.0	40.0	23.0	41.0
45	34.857401360289096	38.0	34.0	40.0	23.0	41.0
46	34.73975028328194	38.0	34.0	40.0	23.0	41.0
47	34.60917940654107	38.0	34.0	40.0	23.0	41.0
48	34.50887348882811	38.0	34.0	40.0	22.0	41.0
49	34.46962521061979	38.0	34.0	40.0	22.0	41.0
50	34.342571583941194	37.0	34.0	40.0	21.0	41.0
51	33.02126824590212	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	10.0
11	6.0
12	16.0
13	20.0
14	26.0
15	43.0
16	61.0
17	130.0
18	338.0
19	617.0
20	1277.0
21	2228.0
22	3239.0
23	4401.0
24	5957.0
25	8988.0
26	12586.0
27	14678.0
28	14757.0
29	14590.0
30	15559.0
31	18676.0
32	23269.0
33	31546.0
34	49938.0
35	53570.0
36	59627.0
37	81341.0
38	140394.0
39	188647.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.961741534356804	17.836583150952443	20.77213416447229	17.429541150218455
2	35.62092991484129	28.78198139313105	20.86441844828464	14.732670243743017
3	27.152607935109014	28.939494191045878	29.7135302957651	14.194367578080005
4	24.16897265759986	21.646356980790404	37.566132605417046	16.61853775619269
5	27.608403897102352	24.80598870086766	29.244340388370844	18.341267013659145
6	23.21277889542811	33.72474906443291	29.87291824603606	13.189553794102915
7	69.46448327498038	5.070679849880659	21.12506462578656	4.339772249352403
8	69.3369729147636	9.391700575671774	16.363918854121184	4.907407655443433
9	63.37546175626769	6.403377417272297	17.475080564581585	12.74608026187842
10	36.84835107138169	24.199376913659947	24.320726058992292	14.631545955966066
11	29.595931455145013	21.80695038614745	30.69195800730238	17.90516015140516
12	24.835857199111715	19.160440714379472	35.06561693851911	20.938085147989703
13	24.754020192712087	20.132305392670297	38.47946574230585	16.634208672311768
14	19.729415515010594	26.39638577777302	34.56361186489259	19.310586842323797
15	17.790374039319264	22.820336295181125	40.4842714899157	18.90501817558391
16	21.964865002424304	24.32635151606068	32.193419286745616	21.515364194769397
17	22.01669957112587	24.3775163874922	33.22180641462833	20.3839776267536
18	22.19952692584844	23.26675649539382	33.35614768699957	21.17756889175817
19	21.473039327302487	25.66735332960089	30.723031960632515	22.13657538246411
20	22.666707741432564	26.32687120114224	33.33458343490408	17.671837622521117
21	21.686940635355196	27.655550584913595	33.755019381039	16.902489398692218
22	20.508541318982168	21.66711759616183	35.52971714666102	22.29462393819498
23	20.521533446021063	25.17472401775501	34.79050529998419	19.513237236239732
24	21.73850732514874	24.320859998446302	32.206143534876496	21.734489141528464
25	21.070551268004813	28.590046155535852	30.196783845830332	20.142618730629007
26	19.591993635197145	23.230860721719353	32.93865840885286	24.23848723423064
27	22.261942711416733	23.78791491094366	31.426615912542893	22.52352646509672
28	19.107668569499843	24.38568669418676	33.69822905253909	22.808415683774307
29	21.139664026273564	22.820470234635135	34.26318566954994	21.776680069541364
30	21.24882468129107	23.760725201779785	32.550233992226154	22.44021612470299
31	24.938588760336778	21.550724210627827	30.884830821075642	22.625856207959753
32	24.346174555254045	23.99418167011784	32.14774593292848	19.51189784169964
33	22.81966659791108	21.790877651666342	30.179103837901117	25.21035191252146
34	23.799701582896468	22.94168544051347	32.59992552966357	20.65868744692649
35	20.93540635890952	26.457863987163243	30.20910627559918	22.39762337832806
36	22.91087936609135	25.847635834697286	31.50805110058049	19.73343369863087
37	20.78566204932722	25.358890767017677	32.81355895880826	21.04188822484684
38	22.19390146878005	24.93323118217641	28.967889355295835	23.904977993747707
39	24.18571508935101	23.35180804868967	30.614674942339065	21.847801919620256
40	22.60308650077819	24.260587244142158	31.014350273102547	22.12197598197711
41	21.18279253046453	25.304913167051968	30.50404095332746	23.008253349156046
42	20.632703192848705	25.68891758169637	32.96330326839056	20.715075957064368
43	22.51120403532787	23.12772734213226	31.50470261423026	22.856366008309603
44	22.559824057133213	22.722292614846385	30.56993916469999	24.14794416332041
45	20.91652089589422	22.664966528530442	29.679375734992753	26.739136840582585
46	23.679022134834167	24.250675724545477	29.46132230386576	22.608979836754596
47	19.642354869904608	23.261666796141473	34.79090711834622	22.305071215607697
48	20.868704510812933	24.71075774906711	30.535382785565613	23.885154954554345
49	20.68453776155027	22.415035507349256	34.39591966847306	22.50450706262741
50	20.306962440698307	22.499819181737088	33.29949129795367	23.893727079610933
51	20.317275778657017	22.464860984240683	30.711647107041735	26.506216130060565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	445.0
1	717.5
2	990.0
3	10116.0
4	19242.0
5	12571.5
6	5901.0
7	5680.0
8	5459.0
9	5564.5
10	5670.0
11	5447.0
12	5224.0
13	5035.5
14	4847.0
15	4578.0
16	4309.0
17	4146.5
18	3984.0
19	3824.5
20	3665.0
21	3596.0
22	3527.0
23	3743.0
24	3959.0
25	4383.0
26	5718.0
27	6629.0
28	7451.5
29	8274.0
30	9435.0
31	10596.0
32	12192.5
33	13789.0
34	15618.0
35	17447.0
36	19362.0
37	21277.0
38	22653.0
39	24029.0
40	26278.5
41	28528.0
42	30631.5
43	32735.0
44	36992.0
45	41249.0
46	70738.0
47	100227.0
48	81508.0
49	62789.0
50	62434.5
51	62080.0
52	55010.0
53	47940.0
54	44054.5
55	40169.0
56	38361.0
57	36553.0
58	33748.0
59	30943.0
60	27664.5
61	24386.0
62	22150.5
63	19915.0
64	17591.5
65	15268.0
66	12823.5
67	10379.0
68	8844.0
69	7309.0
70	6017.5
71	4726.0
72	3887.5
73	3049.0
74	2549.5
75	1603.5
76	1157.0
77	889.0
78	621.0
79	413.0
80	205.0
81	176.5
82	148.0
83	89.5
84	31.0
85	34.0
86	37.0
87	30.0
88	23.0
89	16.5
90	10.0
91	5.0
92	0.0
93	2.0
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	746606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.60325025225664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79638245259598	26.273910721710152
2	8.290569222300634	5.903424215104524
3	3.564088018208834	3.8067935290007555
4	2.2169639420015876	3.1572448810924767
5	1.655508555601803	2.947074269992146
6	1.3509649195732352	2.8859245268151397
7	1.1554651012179378	2.87968192094879
8	0.956887657571167	2.7254648588641532
9	0.9223500573515394	2.955479392086311
>10	6.0217959856068735	33.88309753257256
>50	0.037718081399317545	0.9343802744651462
>100	0.023762390529858627	1.5762798786223462
>500	0.001508723208244986	0.41025174895137656
>1k	0.004526169624734958	3.3623185892780896
>5k	3.771808020612465E-4	1.240558316469007
>10k+	0.0011315424061837396	5.058115344027021
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	15816	2.11838640460966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11682	1.5646807017355875	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	10168	1.361896368365644	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	9238	1.237332676137079	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	4270	0.5719214686193254	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT	3514	0.47066324138836285	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	3108	0.41628382306062367	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	2058	0.27564739635095353	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT	1956	0.26198557204201417	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC	1749	0.23426010506210773	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTG	1735	0.2323849527059788	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	1570	0.2102849427944592	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT	1470	0.19689099739353824	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA	1343	0.1798806867343686	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	1176	0.15751279791483058	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1089	0.14586006541602933	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	904	0.12108126642432555	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	868	0.116259446079994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3393945400920967E-4	0.0	0.0	0.09790974088073227	0.0
2	1.3393945400920967E-4	0.0	0.0	0.7408191201249387	0.0
3	1.3393945400920967E-4	0.0	0.0	1.1680859784143176	0.0
4	1.3393945400920967E-4	0.0	0.0	2.1107518557311353	0.0
5	1.3393945400920967E-4	0.0	0.0	5.211048397682312	0.0
6	2.6787890801841934E-4	0.0	0.0	6.707687856781221	0.0
7	2.6787890801841934E-4	0.0	0.0	7.755362266041259	0.0
8	2.6787890801841934E-4	0.0	0.0	8.856478517450972	0.0
9	2.6787890801841934E-4	0.0	0.0	9.416077556301449	0.0
10	2.6787890801841934E-4	0.0	0.0	11.614425814954608	0.0
11	4.0181836202762903E-4	0.0	0.0	12.449002552885993	0.0
12	4.0181836202762903E-4	0.0	0.0	14.408402825586721	0.0
13	4.0181836202762903E-4	0.0	0.0	14.840223625312413	0.0
14	4.0181836202762903E-4	0.0	0.0	15.13917648666097	0.0
15	4.0181836202762903E-4	0.0	0.0	15.552245762825372	0.0
16	4.0181836202762903E-4	0.0	0.0	15.931830175487473	0.0
17	4.0181836202762903E-4	0.0	0.0	16.32829095935473	0.0
18	4.0181836202762903E-4	0.0	0.0	16.78341722407803	0.0
19	4.0181836202762903E-4	0.0	0.0	17.274171383567772	0.0
20	4.0181836202762903E-4	0.0	0.0	17.613841838935127	0.0
21	4.0181836202762903E-4	0.0	0.0	17.93221592111502	0.0
22	5.357578160368387E-4	0.0	0.0	18.35546459578412	0.0
23	5.357578160368387E-4	0.0	0.0	18.699153234771753	0.0
24	5.357578160368387E-4	0.0	0.0	18.9950254886781	0.0
25	5.357578160368387E-4	0.0	0.0	19.273619553017255	0.0
26	5.357578160368387E-4	0.0	0.0	19.568420291291524	0.0
27	5.357578160368387E-4	0.0	0.0	19.918538024071598	0.0
28	6.696972700460484E-4	0.0	0.0	20.18601511372799	0.0
29	6.696972700460484E-4	0.0	0.0	20.48938797705885	0.0
30	6.696972700460484E-4	0.0	0.0	20.905136042303436	0.0
31	8.036367240552581E-4	0.0	0.0	21.214536181064712	0.0
32	8.036367240552581E-4	0.0	0.0	21.563582398212713	0.0
33	9.375761780644678E-4	0.0	0.0	21.8891892109091	0.0
34	0.0010715156320736773	0.0	0.0	22.24345906676346	0.0
35	0.0010715156320736773	0.0	0.0	22.593174981181505	0.0
36	0.0010715156320736773	0.0	0.0	22.926416342756422	0.0
37	0.0012054550860828872	0.0	0.0	23.2722480130082	0.0
38	0.0012054550860828872	0.0	0.0	23.659600914002834	0.0
39	0.0012054550860828872	0.0	0.0	24.25027390618345	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAG	20	7.031697E-4	45.0	1
CGGTCTA	35	1.2111559E-7	45.0	31
CGATCCA	20	7.031697E-4	45.0	34
CGACTGG	30	2.1644755E-6	44.999996	2
CGTTTTT	6120	0.0	43.713238	1
CGTTAGG	70	0.0	41.785713	2
CGTAAGG	70	0.0	41.785713	2
CGACGAA	65	0.0	41.53846	19
CGATGAA	725	0.0	40.034485	19
TACGGCT	1130	0.0	40.022125	7
TATAGCG	40	3.4560435E-7	39.375	1
CCGATGA	715	0.0	39.335663	18
ACGGCTG	1170	0.0	39.038464	8
ATCGAAT	35	6.245349E-6	38.571426	43
GTTTTTT	7290	0.0	37.716053	2
GAATCTG	2235	0.0	37.34899	1
GATGAAT	835	0.0	36.916172	20
CCACCGA	1155	0.0	36.623375	15
AATCTGT	2290	0.0	36.058952	2
TTGATCG	25	0.0021066365	36.0	1
>>END_MODULE