SRR2935363.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330923
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2353	0.7110415413857605	RNA PCR Primer, Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1933	0.5841237991919571	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	1914	0.5783822822831898	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	1733	0.5236867790996697	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	1542	0.4659694249115353	No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1305	0.3943515561021748	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.2798234030272903	No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	767	0.2317759720539219	Illumina PCR Primer Index 3 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	506	0.15290566083348694	TruSeq Adapter, Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	487	0.14716414392471963	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	398	0.12026966998365178	No Hit
ACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	371	0.112110672271193	TruSeq Adapter, Index 7 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	364	0.10999537656796293	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	340	0.10274293415688845	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACT	25	3.8847662E-5	45.0	6
CGACGGT	20	7.0259976E-4	45.0	28
CTAAGCG	20	7.0259976E-4	45.0	1
CACGACG	20	7.0259976E-4	45.0	26
GCGCATG	25	3.8847662E-5	45.0	1
TGCGGAA	20	7.0259976E-4	45.0	4
GCGTTAG	55	1.8189894E-12	45.0	1
CGGTCTA	20	7.0259976E-4	45.0	31
CGCACGG	25	3.8847662E-5	45.0	2
GGTCTAA	20	7.0259976E-4	45.0	32
TAGTGCG	40	6.7902874E-9	45.0	1
TGAGCGG	45	3.8380676E-10	45.0	2
ATCATCG	25	3.8847662E-5	45.0	10
TAATGCG	20	7.0259976E-4	45.0	1
TAGTATG	20	7.0259976E-4	45.0	1
ACGGTCT	25	3.8847662E-5	45.0	30
CTAGCGC	20	7.0259976E-4	45.0	38
ACGAAAG	35	1.2084274E-7	45.0	1
CGAAAGG	60	0.0	44.999996	2
CGTTAGG	100	0.0	42.75	2