##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330923
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678124518392497	31.0	31.0	34.0	30.0	34.0
2	31.725229131852426	33.0	31.0	34.0	30.0	34.0
3	31.83077332189059	33.0	31.0	34.0	30.0	34.0
4	35.59085648323024	37.0	35.0	37.0	33.0	37.0
5	35.62471934558795	37.0	35.0	37.0	33.0	37.0
6	35.575653550826026	37.0	35.0	37.0	33.0	37.0
7	35.87713758185439	37.0	35.0	37.0	35.0	37.0
8	35.68293832704285	37.0	35.0	37.0	35.0	37.0
9	37.4677130329412	39.0	37.0	39.0	35.0	39.0
10	36.87034143894501	39.0	37.0	39.0	32.0	39.0
11	36.86194673685419	39.0	37.0	39.0	33.0	39.0
12	36.88111735962747	39.0	35.0	39.0	33.0	39.0
13	36.7707261205779	39.0	35.0	39.0	33.0	39.0
14	37.728429272066315	39.0	37.0	41.0	33.0	41.0
15	37.89404786007621	40.0	37.0	41.0	33.0	41.0
16	37.852742178694136	39.0	36.0	41.0	33.0	41.0
17	37.870791694744696	39.0	36.0	41.0	33.0	41.0
18	37.83626100331497	39.0	36.0	41.0	33.0	41.0
19	37.75208734358144	39.0	36.0	41.0	33.0	41.0
20	37.815515996168294	39.0	36.0	41.0	33.0	41.0
21	37.758535973625285	39.0	36.0	41.0	33.0	41.0
22	37.85760735881156	39.0	36.0	41.0	33.0	41.0
23	37.86645836040408	39.0	36.0	41.0	34.0	41.0
24	37.85343418257419	39.0	36.0	41.0	33.0	41.0
25	37.606494562179115	39.0	36.0	41.0	33.0	41.0
26	37.68962266146505	39.0	36.0	41.0	33.0	41.0
27	37.68994902137355	39.0	36.0	41.0	33.0	41.0
28	37.59501152836158	39.0	36.0	41.0	33.0	41.0
29	37.533652843712886	39.0	36.0	41.0	33.0	41.0
30	37.42067490020337	39.0	36.0	41.0	33.0	41.0
31	37.440987782656386	39.0	36.0	41.0	33.0	41.0
32	37.43690526194916	39.0	36.0	41.0	33.0	41.0
33	37.39349637226787	39.0	36.0	41.0	33.0	41.0
34	37.38446103776407	40.0	36.0	41.0	33.0	41.0
35	37.37639571743276	40.0	36.0	41.0	33.0	41.0
36	37.3051072303829	39.0	35.0	41.0	33.0	41.0
37	37.21487173753411	39.0	35.0	41.0	32.0	41.0
38	37.16059022793822	39.0	35.0	41.0	32.0	41.0
39	37.11244911958371	39.0	35.0	41.0	32.0	41.0
40	37.039592291862455	39.0	35.0	41.0	32.0	41.0
41	36.924287523079386	39.0	35.0	41.0	32.0	41.0
42	36.990106459810896	39.0	35.0	41.0	32.0	41.0
43	36.917161998410506	39.0	35.0	41.0	32.0	41.0
44	36.76233141848708	39.0	35.0	41.0	31.0	41.0
45	36.746641968071124	39.0	35.0	41.0	31.0	41.0
46	36.63314124433781	39.0	35.0	40.0	31.0	41.0
47	36.57423328085385	39.0	35.0	40.0	31.0	41.0
48	36.52046548592875	39.0	35.0	40.0	31.0	41.0
49	36.45659564309522	39.0	35.0	40.0	31.0	41.0
50	36.34658817912324	39.0	35.0	40.0	31.0	41.0
51	35.079245020745006	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	7.0
12	4.0
13	6.0
14	7.0
15	10.0
16	15.0
17	38.0
18	62.0
19	133.0
20	255.0
21	399.0
22	629.0
23	947.0
24	1270.0
25	1698.0
26	2141.0
27	2678.0
28	3213.0
29	3895.0
30	4930.0
31	6490.0
32	8671.0
33	12339.0
34	21688.0
35	27521.0
36	26506.0
37	40123.0
38	72068.0
39	93143.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98324987988142	21.13301281567011	24.913046237342222	12.970691067106246
2	30.140244105124154	26.26713767250992	25.610791634307677	17.98182658805825
3	30.913233592104504	25.473297413597724	27.513046841712423	16.100422152585345
4	28.095961900502537	25.25693288166734	28.489406901303326	18.157698316526805
5	25.325226714371624	29.03998815434406	25.854050640179132	19.780734491105182
6	24.820879781701482	34.631016883081564	25.90814177316173	14.63996156205522
7	77.18260743435785	6.015598794885819	11.412020318926155	5.389773451830184
8	76.29448542410168	7.1285465198852895	9.8436796475313	6.733288408481732
9	70.37347056566028	7.947165957035322	12.466948504637031	9.212414972667357
10	36.90072917264741	29.47996966061591	19.182407992191536	14.436893174545135
11	27.9497043118792	24.924529271159756	27.313906860508336	19.81185955645271
12	26.364139089758044	20.39900520664928	32.88499137261538	20.351864330977296
13	23.953004173176236	24.650447385041232	32.87864548550569	18.51790295627684
14	18.100283147439132	29.949565306732985	29.956213378943136	21.993938166884742
15	16.53103592074289	26.453585879494625	37.677042695732844	19.33833550402964
16	18.93068780350716	24.76497553811612	32.63024933292639	23.674087325450333
17	18.691961574142628	25.175342904542745	30.003958624816047	26.128736896498584
18	19.839660585695164	25.59658893458599	31.786850717538524	22.776899762180324
19	22.055583927378876	26.238732273066546	29.070811034591127	22.63487276496345
20	22.32120463068448	26.714069436092387	30.247822000888426	20.716903932334713
21	22.852143852195223	27.662628466440836	29.718695889980452	19.766531791383493
22	18.92222662069424	25.593567083581377	28.138267814567136	27.34593848115725
23	18.40518791380472	28.259746224952632	28.571903433729297	24.76316242751335
24	21.04507695143583	24.852911402350394	29.348216956814728	24.753794689399044
25	19.442589363688832	27.825506235589547	27.562605198188102	25.16929920253352
26	17.34663350688831	27.341405704650327	30.688105692260738	24.62385509620063
27	20.37996754532021	29.03364226723437	28.413558441087506	22.172831746357915
28	18.981757085485143	26.03808136636015	31.81767359778559	23.16248795036912
29	18.870250783414875	25.039359609335104	32.40723672878585	23.683152878464174
30	20.23673180770149	27.8321543077997	29.0898486959202	22.841265188578614
31	23.052190388700694	28.803679405783218	24.804259601176103	23.339870604339982
32	20.580316266926143	28.2271102341028	27.69949504869713	23.49307845027393
33	20.879177331282502	26.51734693569199	25.405003580893442	27.19847215213207
34	19.841473696297932	26.25202841748685	28.779806782846766	25.126691103368458
35	18.536940617605907	25.321902678266543	28.740220534686316	27.40093616944123
36	21.022110883800764	25.81537094732007	27.500052882392577	25.662465286486587
37	19.581292324800632	28.334385944766606	29.662791646395082	22.421530084037677
38	20.173877306805508	26.060745248894758	26.96216340357122	26.803214040728506
39	20.230083735491338	24.62445946640155	28.747472977097395	26.397983821009724
40	20.630781178703202	23.876249157659032	29.52771490648882	25.96525475714894
41	17.427921298912434	26.369276236465883	27.042242455193506	29.160560009428178
42	19.655629859514146	24.76134931691058	28.250680671938788	27.332340151636487
43	22.30669974586233	23.000214551421326	28.399355741365817	26.293729961350525
44	20.84019545332298	23.969019983500694	28.90612015483964	26.284664408336685
45	19.66499759762845	23.957234764582697	28.43350265771796	27.944264980070894
46	22.381037280575843	24.92936423276714	28.01165225747379	24.677946229183224
47	18.508535218162532	24.349471024981643	32.11290844093641	25.029085315919414
48	19.14282174403109	23.65142344291572	29.284153715516904	27.921601097536286
49	19.962347736482506	21.62920075062779	32.210816413485915	26.197635099403787
50	19.827875366777164	21.57178558154012	30.764256337577017	27.836082714105697
51	18.78714988078798	22.112092541165165	27.34049914934894	31.760258428697913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	353.0
2	468.0
3	1060.5
4	1653.0
5	1140.0
6	627.0
7	608.0
8	589.0
9	630.5
10	672.0
11	634.0
12	596.0
13	635.0
14	674.0
15	748.5
16	823.0
17	711.0
18	599.0
19	682.5
20	766.0
21	986.0
22	1206.0
23	1270.5
24	1335.0
25	1624.0
26	2320.5
27	2728.0
28	3257.5
29	3787.0
30	4449.5
31	5112.0
32	5442.0
33	5772.0
34	7005.0
35	8238.0
36	8824.5
37	9411.0
38	10278.0
39	11145.0
40	12623.0
41	14101.0
42	16797.0
43	19493.0
44	22185.0
45	24877.0
46	32607.0
47	40337.0
48	41946.5
49	43556.0
50	40688.0
51	37820.0
52	30435.0
53	23050.0
54	19245.5
55	15441.0
56	13789.0
57	12137.0
58	11532.0
59	10927.0
60	10119.0
61	9311.0
62	8386.5
63	7462.0
64	6178.5
65	4895.0
66	4056.0
67	3217.0
68	2481.5
69	1746.0
70	1803.5
71	1861.0
72	1531.5
73	1202.0
74	901.5
75	452.0
76	303.0
77	192.5
78	82.0
79	73.0
80	64.0
81	58.5
82	53.0
83	31.0
84	9.0
85	7.5
86	6.0
87	4.5
88	3.0
89	9.0
90	15.0
91	7.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.962420312584445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.66393648321082	21.627031778804007
2	13.418820410293788	8.577959561057938
3	4.951881333322139	4.748223375410498
4	2.486998932037097	3.179620207308733
5	1.4863566783285984	2.3753778443577764
6	1.0404249682272053	1.9952700082911148
7	0.7966977716198577	1.782507232702931
8	0.6773368901179245	1.731946110013434
9	0.5201859737179023	1.4963762459414338
>10	6.766393694435919	42.29780747419339
>50	0.12532200495200002	2.747329246397534
>100	0.0566932879544762	3.314470576595775
>500	0.0029838572607619055	0.6990716284226078
>1k	0.005967714521523811	3.4270087105028253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2353	0.7110415413857605	RNA PCR Primer, Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1933	0.5841237991919571	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	1914	0.5783822822831898	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	1733	0.5236867790996697	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	1542	0.4659694249115353	No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1305	0.3943515561021748	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.2798234030272903	No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	767	0.2317759720539219	Illumina PCR Primer Index 3 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	506	0.15290566083348694	TruSeq Adapter, Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	487	0.14716414392471963	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	398	0.12026966998365178	No Hit
ACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	371	0.112110672271193	TruSeq Adapter, Index 7 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	364	0.10999537656796293	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	340	0.10274293415688845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41913073434001263	0.0
2	0.0	0.0	0.0	2.207159973770333	0.0
3	0.0	0.0	0.0	2.9535571719100817	0.0
4	0.0	0.0	0.0	3.8156912635265607	0.0
5	0.0	0.0	0.0	6.271247389876195	0.0
6	0.0	0.0	0.0	7.496003602046398	0.0
7	0.0	0.0	0.0	8.797817014834267	0.0
8	0.0	0.0	0.0	10.77350320165114	0.0
9	0.0	0.0	0.0	11.51597199348489	0.0
10	0.0	0.0	0.0	13.624317439404333	0.0
11	0.0	0.0	0.0	15.805489494534983	0.0
12	0.0	0.0	0.0	18.209674153806173	0.0
13	0.0	0.0	0.0	19.007442819024366	0.0
14	0.0	0.0	0.0	19.373388975683167	0.0
15	0.0	0.0	0.0	19.953886553669584	0.0
16	3.0218510046143665E-4	0.0	0.0	21.022715254001685	0.0
17	3.0218510046143665E-4	0.0	0.0	22.30428226505864	0.0
18	3.0218510046143665E-4	0.0	0.0	23.830619207489356	0.0
19	3.0218510046143665E-4	0.0	0.0	24.724180549553825	0.0
20	3.0218510046143665E-4	0.0	0.0	25.549145873813547	0.0
21	3.0218510046143665E-4	0.0	0.0	26.443311586078938	0.0
22	3.0218510046143665E-4	0.0	0.0	27.419067275468915	0.0
23	3.0218510046143665E-4	0.0	0.0	28.358862937903982	0.0
24	3.0218510046143665E-4	0.0	0.0	29.05902581567313	0.0
25	3.0218510046143665E-4	0.0	0.0	29.736222625807212	0.0
26	3.0218510046143665E-4	0.0	0.0	30.317626759095017	0.0
27	3.0218510046143665E-4	0.0	0.0	30.9407324362465	0.0
28	3.0218510046143665E-4	0.0	0.0	31.529389011945376	0.0
29	3.0218510046143665E-4	0.0	0.0	32.13557232347102	0.0
30	6.043702009228733E-4	0.0	0.0	32.872299598396005	0.0
31	9.0655530138431E-4	0.0	0.0	33.46276928469765	0.0
32	9.0655530138431E-4	0.0	0.0	34.0876880724519	0.0
33	9.0655530138431E-4	0.0	0.0	34.6965910498817	0.0
34	9.0655530138431E-4	0.0	0.0	35.30579621241195	0.0
35	9.0655530138431E-4	0.0	0.0	35.95549417840404	0.0
36	9.0655530138431E-4	0.0	0.0	36.54173327329923	0.0
37	0.0012087404018457466	0.0	0.0	37.14912532522671	0.0
38	0.0012087404018457466	0.0	0.0	37.692151950755914	0.0
39	0.0012087404018457466	0.0	0.0	38.28503911786125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACT	25	3.8847662E-5	45.0	6
CGACGGT	20	7.0259976E-4	45.0	28
CTAAGCG	20	7.0259976E-4	45.0	1
CACGACG	20	7.0259976E-4	45.0	26
GCGCATG	25	3.8847662E-5	45.0	1
TGCGGAA	20	7.0259976E-4	45.0	4
GCGTTAG	55	1.8189894E-12	45.0	1
CGGTCTA	20	7.0259976E-4	45.0	31
CGCACGG	25	3.8847662E-5	45.0	2
GGTCTAA	20	7.0259976E-4	45.0	32
TAGTGCG	40	6.7902874E-9	45.0	1
TGAGCGG	45	3.8380676E-10	45.0	2
ATCATCG	25	3.8847662E-5	45.0	10
TAATGCG	20	7.0259976E-4	45.0	1
TAGTATG	20	7.0259976E-4	45.0	1
ACGGTCT	25	3.8847662E-5	45.0	30
CTAGCGC	20	7.0259976E-4	45.0	38
ACGAAAG	35	1.2084274E-7	45.0	1
CGAAAGG	60	0.0	44.999996	2
CGTTAGG	100	0.0	42.75	2
>>END_MODULE