##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935362.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591750
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79099281791297	33.0	31.0	34.0	30.0	34.0
2	31.87916518800169	33.0	31.0	34.0	30.0	34.0
3	31.910546683565695	33.0	31.0	34.0	30.0	34.0
4	35.64662779890156	37.0	35.0	37.0	33.0	37.0
5	35.63594592310942	37.0	35.0	37.0	33.0	37.0
6	35.540616814533166	37.0	35.0	37.0	33.0	37.0
7	35.82786142796789	37.0	35.0	37.0	35.0	37.0
8	35.55429995775243	37.0	35.0	37.0	33.0	37.0
9	37.39416645542881	39.0	37.0	39.0	34.0	39.0
10	36.97779129700042	39.0	37.0	39.0	32.0	39.0
11	36.92934854245881	39.0	37.0	39.0	33.0	39.0
12	36.94610054921842	39.0	37.0	39.0	33.0	39.0
13	36.842867765103506	39.0	37.0	39.0	33.0	39.0
14	37.898654837346854	40.0	37.0	41.0	33.0	41.0
15	37.92143134769751	40.0	37.0	41.0	32.0	41.0
16	37.97576679340938	40.0	37.0	41.0	33.0	41.0
17	37.892821292775665	40.0	37.0	41.0	33.0	41.0
18	37.62517279256443	39.0	37.0	40.0	33.0	41.0
19	37.279442332065905	38.0	36.0	40.0	33.0	41.0
20	36.927379805661175	38.0	35.0	40.0	32.0	41.0
21	37.027922264469794	38.0	35.0	40.0	32.0	41.0
22	37.158403041825096	39.0	35.0	40.0	33.0	41.0
23	37.164814533164346	39.0	35.0	40.0	33.0	41.0
24	37.10969328263625	39.0	35.0	41.0	33.0	41.0
25	36.89830502746092	38.0	35.0	40.0	32.0	41.0
26	36.88509505703422	38.0	35.0	40.0	33.0	41.0
27	36.90667173637516	38.0	35.0	40.0	33.0	41.0
28	36.72897338403042	38.0	35.0	40.0	32.0	41.0
29	36.589284326151244	38.0	35.0	40.0	32.0	41.0
30	36.39120405576679	38.0	35.0	40.0	31.0	41.0
31	36.18237600337981	38.0	35.0	40.0	30.0	41.0
32	35.792217997465144	38.0	35.0	40.0	29.0	41.0
33	35.2182594000845	38.0	35.0	40.0	24.0	41.0
34	34.779143219264895	38.0	35.0	40.0	21.0	41.0
35	34.52051880016899	38.0	34.0	40.0	18.0	41.0
36	34.33084579636671	38.0	34.0	40.0	18.0	41.0
37	34.248991972961555	38.0	34.0	40.0	16.0	41.0
38	34.03937980566118	38.0	34.0	40.0	15.0	41.0
39	33.91305111956063	38.0	34.0	40.0	15.0	41.0
40	33.72014533164343	38.0	33.0	40.0	15.0	41.0
41	33.548697929869036	38.0	33.0	40.0	13.0	41.0
42	33.616508660752004	38.0	33.0	40.0	13.0	41.0
43	33.57721166032953	38.0	33.0	40.0	12.0	41.0
44	33.43209801436417	38.0	33.0	40.0	12.0	41.0
45	33.45645627376426	38.0	33.0	40.0	12.0	41.0
46	33.33476468103084	37.0	33.0	40.0	12.0	41.0
47	33.2158783269962	37.0	33.0	40.0	12.0	41.0
48	33.1674085340093	37.0	33.0	40.0	12.0	41.0
49	33.11745162653148	37.0	33.0	40.0	12.0	41.0
50	33.00081115335868	37.0	32.0	40.0	10.0	41.0
51	31.904466413181243	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	5.0
10	4.0
11	3.0
12	3.0
13	8.0
14	27.0
15	28.0
16	62.0
17	122.0
18	335.0
19	596.0
20	1223.0
21	1965.0
22	3030.0
23	4254.0
24	6371.0
25	10052.0
26	15602.0
27	17721.0
28	16600.0
29	14615.0
30	14281.0
31	15788.0
32	18910.0
33	24346.0
34	37059.0
35	41427.0
36	49101.0
37	61313.0
38	104924.0
39	131916.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94862695395015	16.297422898183356	19.142205323193917	24.61174482467258
2	39.99594423320659	28.138572032108154	19.151837769328264	12.713645965356992
3	23.363413603717788	28.236586396282213	36.23236163920575	12.167638360794255
4	21.000591465990706	19.452978453738908	44.74575411913815	14.800675961132233
5	25.208280523869874	22.23675538656527	35.774228981833545	16.780735107731306
6	20.04630333755809	30.956653992395438	36.22610899873257	12.7709336713139
7	63.09742289818335	4.054921841994085	28.67984790874525	4.167807351077313
8	62.74490916772285	8.282382762991128	23.921419518377693	5.051288550908323
9	57.556231516687795	5.227038445289396	24.62391212505281	12.592817912970004
10	32.989607097591886	23.37220109843684	30.06269539501479	13.575496408956486
11	24.62661596958175	19.96062526404732	37.66489226869455	17.747866497676384
12	20.846810308407264	17.222306717363754	41.90637938318547	20.024503591043516
13	21.261681453316434	18.334431770173214	45.13003802281369	15.273848753696662
14	16.756907477820025	25.035910435149976	40.5433037600338	17.663878326996198
15	15.03844528939586	22.36147021546261	45.05399239543726	17.546092099704268
16	18.663455851288553	23.790283058724125	37.45703422053232	20.089226869455008
17	18.47266582171525	24.38783269961977	37.99002957329953	19.14947190536544
18	18.699450781580058	23.346176594845797	37.682805238698776	20.271567384875368
19	17.97853823405154	25.164174059991552	35.363075623151666	21.494212082805237
20	19.173637515842838	25.668103084072662	38.238952260245036	16.91930713983946
21	18.670891423743136	26.27275031685678	39.18073510773131	15.87562315166878
22	17.611153358681875	20.319898605830165	40.94076890578792	21.128179129700044
23	18.233882551753275	23.815631601182933	39.8653147444022	18.085171102661597
24	20.049514152936208	23.569074778200253	37.04554288128433	19.335868187579212
25	18.845458386142795	28.07182087029996	34.5723700887199	18.510350654837346
26	17.721504013519223	22.905111956062527	36.61596958174905	22.757414448669202
27	20.71128010139417	22.520321081537812	35.405154203633295	21.36324461343473
28	16.91254752851711	24.958174904942965	36.70519645120406	21.42408111533587
29	18.81301225179552	25.67790452049007	34.99112801013942	20.51795521757499
30	18.032108153781156	25.778115758343894	35.86142796789184	20.3283481199831
31	19.998310097169416	25.147613012251796	33.776256865230245	21.077820025348544
32	23.542374313476973	24.992479932403885	33.428474862695396	18.036670891423743
33	20.915927334178285	22.10190114068441	35.979552175749895	21.002619349387412
34	18.67528517110266	24.69302915082383	37.78555133079848	18.846134347275033
35	19.269455006337136	23.797042670046473	33.25982256020279	23.673679763413606
36	22.098352344740178	27.560963244613433	31.537980566117447	18.80270384452894
37	21.021715251373045	24.49243768483312	33.813941698352345	20.671905365441486
38	20.32682720743557	25.775411913814956	31.990198563582595	21.907562315166878
39	23.61977186311787	23.300211237853823	32.53096746937051	20.549049429657796
40	21.084410646387834	23.064469792986902	35.13206590621039	20.71905365441487
41	19.93747359526827	25.821208280523873	33.16670891423743	21.074609209970426
42	19.545754119138152	24.852217997465146	34.572032108153785	21.029995775242924
43	22.979467680608366	23.65711871567385	31.836417405999157	21.52699619771863
44	21.38149556400507	23.294972539079005	32.03143219264892	23.292099704267006
45	20.007942543303763	22.58538234051542	31.23599493029151	26.17068018588931
46	23.775580904098014	24.066582171525138	30.8871989860583	21.270637938318547
47	18.747613012251797	23.365779467680607	36.63168567807351	21.254921841994083
48	19.30612589776088	24.597380650612592	32.705534431770175	23.390959019856357
49	19.116011829319817	22.128770595690746	36.450528094634564	22.30468948035488
50	19.043514997887623	22.346430080270384	36.13722010984368	22.47283481199831
51	18.5346852555978	22.00929446556823	33.69260667511618	25.763413603717787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	388.0
1	619.0
2	850.0
3	15163.5
4	29477.0
5	19152.5
6	8828.0
7	8538.0
8	8248.0
9	8182.0
10	8116.0
11	8065.5
12	8015.0
13	7714.0
14	7413.0
15	7034.5
16	6656.0
17	6221.5
18	5787.0
19	5357.5
20	4928.0
21	4844.0
22	4760.0
23	4497.0
24	4234.0
25	4310.0
26	4902.5
27	5419.0
28	5921.0
29	6423.0
30	7034.5
31	7646.0
32	8713.0
33	9780.0
34	11132.0
35	12484.0
36	13323.0
37	14162.0
38	15380.0
39	16598.0
40	18417.0
41	20236.0
42	21687.5
43	23139.0
44	26260.5
45	29382.0
46	58090.5
47	86799.0
48	68407.5
49	50016.0
50	49134.0
51	48252.0
52	41282.5
53	34313.0
54	31010.5
55	27708.0
56	25678.0
57	23648.0
58	22188.0
59	20728.0
60	18162.5
61	15597.0
62	13857.5
63	12118.0
64	10501.5
65	8885.0
66	7261.0
67	5637.0
68	4781.0
69	3925.0
70	3310.5
71	2696.0
72	2208.5
73	1721.0
74	1436.5
75	915.0
76	678.0
77	483.5
78	289.0
79	203.5
80	118.0
81	97.0
82	76.0
83	49.5
84	23.0
85	15.5
86	8.0
87	5.0
88	2.0
89	2.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.77526950844973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02511181603614	33.885194437721815
2	10.202227950841502	9.340194680728173
3	4.8953986499778495	6.722645776621111
4	3.109794378568793	5.694067031793939
5	2.1586982360027167	4.940749677021969
6	1.5034388791254443	4.129219192886929
7	1.0879688952468887	3.4861448577715657
8	0.8284102826987525	3.0336563163285146
9	0.5631724987705256	2.32014356198711
>10	1.5679811796052447	10.200093389693857
>50	0.03112158727179416	0.9565164987701947
>100	0.019265744228121322	1.8970910345266045
>500	0.0018524754065501268	0.5586463318192952
>1k	0.0037049508131002536	3.2823439909018335
>5k	0.001111485243930076	3.3718902153100263
>10k+	7.409901626200506E-4	6.181403006117082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19121	3.231263202365864	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	17327	2.928094634558513	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCG	7511	1.2692860160540769	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	7057	1.1925644275454161	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCT	5314	0.8980143641740599	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTT	3724	0.6293198141106886	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	2879	0.48652302492606675	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTT	2414	0.40794254330376	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT	1742	0.2943810730882974	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTC	1678	0.2835656949725391	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG	1603	0.2708914237431348	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	1592	0.26903253062948884	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT	1424	0.24064216307562317	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTTCGT	1270	0.21461765948457964	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCT	1028	0.1737220109843684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTA	867	0.14651457541191382	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCC	755	0.12758766370933672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6899028305872413E-4	0.0	0.0	0.08043937473595268	0.0
2	1.6899028305872413E-4	0.0	0.0	0.7386565272496831	0.0
3	3.3798056611744826E-4	0.0	0.0	1.1685678073510772	0.0
4	3.3798056611744826E-4	0.0	0.0	2.4929446556822983	0.0
5	3.3798056611744826E-4	0.0	0.0	6.335952682720744	0.0
6	3.3798056611744826E-4	0.0	0.0	8.385466835656949	0.0
7	3.3798056611744826E-4	0.0	0.0	9.674862695395015	0.0
8	3.3798056611744826E-4	0.0	0.0	11.08998732572877	0.0
9	3.3798056611744826E-4	0.0	0.0	11.876637093367131	0.0
10	3.3798056611744826E-4	0.0	0.0	14.0397127165188	0.0
11	3.3798056611744826E-4	0.0	0.0	14.853400929446558	0.0
12	3.3798056611744826E-4	0.0	0.0	16.71634980988593	0.0
13	3.3798056611744826E-4	0.0	0.0	17.217574989438106	0.0
14	3.3798056611744826E-4	0.0	0.0	17.604055766793408	0.0
15	3.3798056611744826E-4	0.0	0.0	17.98394592310942	0.0
16	3.3798056611744826E-4	0.0	0.0	18.31263202365864	0.0
17	3.3798056611744826E-4	0.0	0.0	18.67782002534854	0.0
18	3.3798056611744826E-4	0.0	0.0	19.053654414871144	0.0
19	3.3798056611744826E-4	0.0	0.0	19.5389945078158	0.0
20	3.3798056611744826E-4	0.0	0.0	19.832699619771862	0.0
21	3.3798056611744826E-4	0.0	0.0	20.084495141529363	0.0
22	3.3798056611744826E-4	0.0	0.0	20.39036755386565	0.0
23	3.3798056611744826E-4	0.0	0.0	20.66125897760879	0.0
24	3.3798056611744826E-4	0.0	0.0	20.914406421630755	0.0
25	3.3798056611744826E-4	0.0	0.0	21.148964934516265	0.0
26	3.3798056611744826E-4	0.0	0.0	21.418166455428814	0.0
27	3.3798056611744826E-4	0.0	0.0	21.731981411068862	0.0
28	3.3798056611744826E-4	0.0	0.0	21.978369243768483	0.0
29	3.3798056611744826E-4	0.0	0.0	22.23422053231939	0.0
30	3.3798056611744826E-4	0.0	0.0	22.580650612589775	0.0
31	3.3798056611744826E-4	0.0	0.0	22.869792986903253	0.0
32	3.3798056611744826E-4	0.0	0.0	23.175665399239545	0.0
33	3.3798056611744826E-4	0.0	0.0	23.46227291930714	0.0
34	3.3798056611744826E-4	0.0	0.0	23.79095901985636	0.0
35	5.069708491761723E-4	0.0	0.0	24.106463878326995	0.0
36	5.069708491761723E-4	0.0	0.0	24.39476130122518	0.0
37	6.759611322348965E-4	0.0	0.0	24.70063371356147	0.0
38	6.759611322348965E-4	0.0	0.0	25.061427967891845	0.0
39	6.759611322348965E-4	0.0	0.0	25.589015631601182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATC	20	7.030509E-4	45.000004	45
CGGTCTA	40	6.8048394E-9	45.000004	31
CGCTTAT	20	7.030509E-4	45.000004	31
TTAAGCG	30	2.1637097E-6	44.999996	1
CGTTTTT	9225	0.0	44.26829	1
CCGATGA	585	0.0	40.769234	18
TAGCCGT	50	1.0804797E-9	40.5	44
GTAGCCG	50	1.0804797E-9	40.5	43
CGATGAA	600	0.0	40.125004	19
TCACGAC	45	1.9255822E-8	40.0	25
CGACGGT	45	1.9255822E-8	40.0	28
CGTTAGG	45	1.9255822E-8	40.0	2
GTTTTTT	10285	0.0	39.90277	2
GCGAACC	40	3.4544246E-7	39.375004	33
GTTACGG	40	3.4544246E-7	39.375004	2
AAGCACG	40	3.4544246E-7	39.375004	1
CGTTTTC	180	0.0	38.75	1
CGTTTCT	320	0.0	38.67188	1
TACGGCT	955	0.0	38.63874	7
GCCGATG	35	6.2431536E-6	38.571426	9
>>END_MODULE