##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395354
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.457202911820797	31.0	31.0	34.0	30.0	34.0
2	31.499564946857753	31.0	31.0	34.0	30.0	34.0
3	31.591710214137205	31.0	31.0	34.0	30.0	34.0
4	35.3954481300303	37.0	35.0	37.0	33.0	37.0
5	35.408942365576166	37.0	35.0	37.0	33.0	37.0
6	35.363193492414396	37.0	35.0	37.0	33.0	37.0
7	35.62967113017701	37.0	35.0	37.0	33.0	37.0
8	35.462790309444195	37.0	35.0	37.0	33.0	37.0
9	37.20538555320042	39.0	37.0	39.0	34.0	39.0
10	36.431863595663636	39.0	35.0	39.0	32.0	39.0
11	36.54435771485808	38.0	35.0	39.0	32.0	39.0
12	36.63207909873177	39.0	35.0	39.0	32.0	39.0
13	36.55812259392848	39.0	35.0	39.0	32.0	39.0
14	37.517796708772394	39.0	36.0	41.0	32.0	41.0
15	37.61926526606535	39.0	36.0	41.0	32.0	41.0
16	37.6109132574857	39.0	36.0	41.0	33.0	41.0
17	37.557406779746756	39.0	36.0	41.0	32.0	41.0
18	37.529429321570035	39.0	36.0	40.0	32.0	41.0
19	37.43012844185211	39.0	36.0	40.0	32.0	41.0
20	37.39185641222803	39.0	36.0	40.0	32.0	41.0
21	37.42025880603206	39.0	36.0	40.0	32.0	41.0
22	37.54131234286235	39.0	36.0	40.0	33.0	41.0
23	37.587832676537985	39.0	36.0	41.0	33.0	41.0
24	37.608796167485345	39.0	36.0	41.0	33.0	41.0
25	37.30800750719608	39.0	35.0	40.0	32.0	41.0
26	37.4327615251142	39.0	36.0	41.0	33.0	41.0
27	37.40565164384324	39.0	36.0	41.0	33.0	41.0
28	37.0618812507272	39.0	35.0	41.0	31.0	41.0
29	36.81754073564451	39.0	35.0	41.0	31.0	41.0
30	36.762390161728476	39.0	35.0	40.0	31.0	41.0
31	36.79332952240271	39.0	35.0	40.0	31.0	41.0
32	36.85773762248517	39.0	35.0	40.0	31.0	41.0
33	36.7632703855279	39.0	35.0	41.0	31.0	41.0
34	36.70827663309338	39.0	35.0	41.0	31.0	41.0
35	36.70685259286614	39.0	35.0	41.0	31.0	41.0
36	36.57607106542491	39.0	35.0	41.0	30.0	41.0
37	36.57060254860201	39.0	35.0	41.0	30.0	41.0
38	36.44063548111313	39.0	35.0	40.0	30.0	41.0
39	36.45256403122265	39.0	35.0	40.0	30.0	41.0
40	36.40061818016259	39.0	35.0	40.0	30.0	41.0
41	36.21725339822033	39.0	35.0	40.0	30.0	41.0
42	36.24798282045964	39.0	35.0	40.0	30.0	41.0
43	36.13424171754934	39.0	35.0	40.0	29.0	41.0
44	36.026624240554035	39.0	35.0	40.0	29.0	41.0
45	36.074515497503505	39.0	35.0	40.0	29.0	41.0
46	35.921257910631994	39.0	35.0	40.0	29.0	41.0
47	35.93869038886669	39.0	35.0	40.0	29.0	41.0
48	35.88230041937099	38.0	35.0	40.0	29.0	41.0
49	35.679188777652435	39.0	35.0	40.0	27.0	41.0
50	35.485693833880525	38.0	35.0	40.0	27.0	41.0
51	34.26696580785828	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	5.0
13	4.0
14	14.0
15	15.0
16	27.0
17	44.0
18	110.0
19	256.0
20	484.0
21	868.0
22	1327.0
23	1842.0
24	2433.0
25	3198.0
26	4158.0
27	4866.0
28	5312.0
29	5969.0
30	7246.0
31	9453.0
32	12243.0
33	16461.0
34	25514.0
35	32133.0
36	33710.0
37	48727.0
38	84538.0
39	94362.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67466624847605	21.35630346474299	25.389397856098583	10.579632430682375
2	29.263647262959267	29.816063578463858	24.751488539384955	16.168800619191913
3	27.146810200478562	28.952533678677845	28.838458697774655	15.062197423068946
4	25.1658007760134	24.103461707735345	33.45735720392357	17.273380312327685
5	26.23876323497423	27.823419011822313	26.838984808551324	19.098832944652134
6	22.29799116740946	35.923754407442445	27.09723437729225	14.681020047855844
7	74.85266368874477	5.008928706931004	14.942557808950966	5.1958497953732605
8	74.91918634944885	8.261962696722431	10.563950282531605	6.254900671297116
9	68.953140729574	7.098448479084567	12.39699105105804	11.551419740283391
10	34.638577072699405	28.913075370427517	20.786687373847236	15.661660183025845
11	25.01074985961948	27.197144837285066	28.55567415531397	19.23643114778148
12	22.669051027686578	20.699170869650995	35.4548075901597	21.17697051250272
13	23.79462456431451	25.80168658973983	34.675506002215734	15.728182843729924
14	18.182691967199016	33.21200746672602	29.660759724196545	18.944540841878418
15	15.135549406354812	27.074469968686294	39.52179565655084	18.26818496840806
16	17.03207758110453	28.378617643934295	30.04016653429585	24.54913824066533
17	17.91204844266151	26.30123888970391	28.703389873379297	27.083322794255277
18	22.092099738462238	25.345133728253664	30.626729462709367	21.936037070574727
19	21.418273243725878	28.584762010754915	27.901070938955975	22.095893806563232
20	19.6644020295735	27.550499046424214	31.064817859437365	21.720281064564922
21	20.07441432235414	30.87258507565372	30.303221922631362	18.749778679360777
22	16.94456107690829	27.37217784567754	29.349393202041714	26.33386787537245
23	15.478280224811181	28.99831543376316	29.284135230704635	26.239269110721025
24	18.68654421101089	25.678252907520854	28.98693322946018	26.64826965200807
25	15.3583876728198	32.72586087405212	26.894884078572623	25.02086737455546
26	15.93761540290474	28.990727297561175	28.82935293433227	26.242304365201818
27	20.359222367802023	28.153755874482112	27.074217030812893	24.41280472690298
28	18.106810605179156	26.269368717655567	33.3344293974514	22.289391279713875
29	20.106031556529086	26.309585839526093	30.10694213287332	23.477440471071496
30	22.618463453006672	28.26125447067691	28.133014968863346	20.987267107453068
31	20.470262094224417	29.26010613273168	26.55620026609064	23.71343150695326
32	22.046317983376923	31.884842444998657	25.169341906240987	20.89949766538343
33	20.143719299665616	25.07120201136197	27.673426852896394	27.11165183607602
34	19.55867399849249	27.290478912569494	27.511293676047288	25.639553412890727
35	19.74028339159336	29.18751296306601	25.6534649959277	25.41873864941293
36	19.102879950626527	27.559098934119802	31.697668418683	21.64035269657067
37	20.606595607986765	27.36990140481695	30.98564830506331	21.037854682132977
38	18.398953848955617	27.083828670002074	27.975940549482235	26.54127693156007
39	23.473140527223705	22.83649589987707	28.08950965463863	25.600853918260597
40	21.115253671393234	25.234346939704672	27.9964285172276	25.6539708716745
41	17.176505106815664	26.64675202476768	28.004522529176384	28.17222033924028
42	20.213783090597286	27.04209392089115	30.861455809224136	21.882667179287424
43	21.29003374191231	25.1088897544985	30.518472052894367	23.08260445069482
44	20.159148509942987	23.533845616839592	28.894357967795948	27.412647905421473
45	19.3365945456477	23.010770094649352	26.860990403537084	30.791644956165864
46	23.282931246427253	26.304021206311308	26.2526748180112	24.16037272925024
47	16.725011002797494	24.261042002863256	33.02255699954977	25.991389994789483
48	19.637843552866546	23.863170728005787	29.38404569069745	27.11494002843022
49	20.55651390905366	20.30939360674231	34.492378981874474	24.64171350232956
50	20.094649352226106	20.334181518335466	32.6780556159796	26.89311351345882
51	19.809841306778228	21.071242481421713	27.461211977114182	31.657704234685873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	268.0
1	327.5
2	387.0
3	1400.0
4	2413.0
5	1685.5
6	958.0
7	955.5
8	953.0
9	985.0
10	1017.0
11	1075.5
12	1134.0
13	1144.5
14	1155.0
15	1156.5
16	1158.0
17	1140.5
18	1123.0
19	1173.0
20	1223.0
21	1231.5
22	1240.0
23	1346.5
24	1453.0
25	1830.0
26	2297.0
27	2387.0
28	3090.5
29	3794.0
30	4414.0
31	5034.0
32	6110.5
33	7187.0
34	8614.0
35	10041.0
36	11097.0
37	12153.0
38	13127.5
39	14102.0
40	16033.0
41	17964.0
42	21625.5
43	25287.0
44	28490.5
45	31694.0
46	48304.5
47	64915.0
48	59992.0
49	55069.0
50	50636.0
51	46203.0
52	36868.0
53	27533.0
54	22407.0
55	17281.0
56	14576.5
57	11872.0
58	10575.0
59	9278.0
60	8243.0
61	7208.0
62	5777.0
63	4346.0
64	3311.0
65	2276.0
66	1706.5
67	1137.0
68	884.5
69	632.0
70	598.0
71	564.0
72	426.0
73	288.0
74	224.0
75	129.5
76	99.0
77	92.5
78	86.0
79	63.0
80	40.0
81	27.5
82	15.0
83	11.5
84	8.0
85	5.5
86	3.0
87	3.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.39493108495501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89880065312894	35.36210687249437
2	10.54294353006299	9.571923899595635
3	3.636269689438899	4.952046358751687
4	1.8008159145490932	3.269916573505853
5	1.061348208757132	2.408991439683523
6	0.7443686346739466	2.027433772369552
7	0.521980249807777	1.6586680227412147
8	0.43000814459962955	1.5616152072055642
9	0.33414765411023095	1.3651748757479987
>10	2.926445775350816	25.266326406844737
>50	0.0598220400581443	1.86565452364694
>100	0.03410415367800748	2.608769262490395
>500	0.0027954224326235642	0.9720387465062954
>1k	0.004472675892197703	2.9859101442941425
>5k	0.0016772534595741384	4.123423894122136
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC	5543	1.402034632253626	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC	5408	1.3678880193446885	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG	5296	1.3395589775239405	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	2655	0.671550053875767	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	1567	0.39635364761707226	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.37687743136530805	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT	1425	0.3604364695943382	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTT	1380	0.34905426529135913	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCT	1157	0.29264911952326267	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCT	1080	0.27317290327149846	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCCATT	1011	0.25572019000693047	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	947	0.23953216610936023	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTA	874	0.2210677013511941	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTC	789	0.19956798211223362	No Hit
ACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	624	0.15783323300131022	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG	596	0.15075097254612324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC	459	0.11609848389038684	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	428	0.10825740981500125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.529378733995356E-4	0.0	0.0	0.3821891267066983	0.0
2	2.529378733995356E-4	0.0	0.0	1.9567273886188075	0.0
3	2.529378733995356E-4	0.0	0.0	2.6892354699838625	0.0
4	2.529378733995356E-4	0.0	0.0	3.8003915478280224	0.0
5	2.529378733995356E-4	0.0	0.0	7.205188261659171	0.0
6	2.529378733995356E-4	0.0	0.0	8.859907829438933	0.0
7	2.529378733995356E-4	0.0	0.0	10.350976593129195	0.0
8	2.529378733995356E-4	0.0	0.0	12.411914385588611	0.0
9	2.529378733995356E-4	0.0	0.0	13.121910996221109	0.0
10	2.529378733995356E-4	0.0	0.0	16.191059152051075	0.0
11	2.529378733995356E-4	0.0	0.0	18.613698103471826	0.0
12	2.529378733995356E-4	0.0	0.0	21.92844893437274	0.0
13	2.529378733995356E-4	0.0	0.0	22.933371105389096	0.0
14	2.529378733995356E-4	0.0	0.0	23.365388993155502	0.0
15	2.529378733995356E-4	0.0	0.0	24.11231453330433	0.0
16	2.529378733995356E-4	0.0	0.0	25.366633447492628	0.0
17	2.529378733995356E-4	0.0	0.0	26.95002453497372	0.0
18	2.529378733995356E-4	0.0	0.0	28.836688132660857	0.0
19	2.529378733995356E-4	0.0	0.0	30.09783636943094	0.0
20	2.529378733995356E-4	0.0	0.0	31.060770853462973	0.0
21	2.529378733995356E-4	0.0	0.0	32.26526100659156	0.0
22	2.529378733995356E-4	0.0	0.0	33.445216186000394	0.0
23	2.529378733995356E-4	0.0	0.0	34.70560560915028	0.0
24	2.529378733995356E-4	0.0	0.0	35.59974099161764	0.0
25	2.529378733995356E-4	0.0	0.0	36.434183035962704	0.0
26	2.529378733995356E-4	0.0	0.0	37.21297874815988	0.0
27	2.529378733995356E-4	0.0	0.0	38.0727145798449	0.0
28	2.529378733995356E-4	0.0	0.0	38.87832170662242	0.0
29	2.529378733995356E-4	0.0	0.0	39.67330544271716	0.0
30	5.058757467990712E-4	0.0	0.0	40.632951734395	0.0
31	5.058757467990712E-4	0.0	0.0	41.510899092964785	0.0
32	5.058757467990712E-4	0.0	0.0	42.30411226394573	0.0
33	5.058757467990712E-4	0.0	0.0	43.095048994066076	0.0
34	5.058757467990712E-4	0.0	0.0	43.92873222479095	0.0
35	5.058757467990712E-4	0.0	0.0	44.713851383823105	0.0
36	5.058757467990712E-4	0.0	0.0	45.4235950565822	0.0
37	5.058757467990712E-4	0.0	0.0	46.123980028025514	0.0
38	5.058757467990712E-4	0.0	0.0	46.84738234594819	0.0
39	5.058757467990712E-4	0.0	0.0	47.659818795307494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	65	0.0	45.000004	2
GTTTGCG	20	7.0276676E-4	45.0	1
CTTCGCT	20	7.0276676E-4	45.0	2
ACGCCTA	20	7.0276676E-4	45.0	30
CGTATGG	35	1.2092278E-7	45.0	2
CAACGAG	20	7.0276676E-4	45.0	14
GCGAACG	40	6.7957444E-9	45.0	1
TCTTCGC	20	7.0276676E-4	45.0	1
TAGCGGA	20	7.0276676E-4	45.0	3
CCTACCG	35	1.2092278E-7	45.0	23
TAAGTAG	20	7.0276676E-4	45.0	1
ATATGCG	25	3.886147E-5	45.0	1
CGACAGG	40	6.7957444E-9	45.0	2
CGCTCCA	20	7.0276676E-4	45.0	12
CGCTAGG	20	7.0276676E-4	45.0	2
CTGTTCG	20	7.0276676E-4	45.0	13
AGCGACT	20	7.0276676E-4	45.0	19
CGATGTA	20	7.0276676E-4	45.0	10
CGGTAGT	20	7.0276676E-4	45.0	29
GGTTCCG	20	7.0276676E-4	45.0	9
>>END_MODULE