FastQCFastQC Report
Sat 14 Jan 2017
SRR2935358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504676
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC27150.5379689147096355No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC26520.525485658125213No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG23700.46960822389017903No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC19070.3778661953411694No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC13600.2694798246795964No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.23837075668349592No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT11580.2294541448374799Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT9860.1953728728927074No Hit
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC7440.14742131585413215No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT5810.11512336627856289No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT5460.10818822373166151No Hit
ACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC5150.102045668904406No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.0295203E-445.00000414
CGACAAT207.0295203E-445.00000420
CCTCGAC207.0295203E-445.00000430
TTGTGCG453.8380676E-1045.01
TCCTCGC253.887686E-545.021
CACGACG253.887686E-545.026
CCTCGCC253.887686E-545.012
GTTAACG253.887686E-545.01
CTAGTCG253.887686E-545.043
ACGCTAG253.887686E-545.01
CGATGTA351.2101191E-745.010
TCGACGT253.887686E-545.026
TAGTCGA253.887686E-545.044
TGCGGTG302.163073E-644.9999964
TCTAGCG302.163073E-644.9999961
GCGATTC302.163073E-644.9999969
CAATGCG302.163073E-644.9999961
CGATCAA302.163073E-644.99999610
TACGGGC750.042.04
CGTTTTT6350.041.456691
Produced by FastQC (version 0.11.5)