##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436355
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.990750650273288	33.0	31.0	34.0	30.0	34.0
2	32.052198324758514	33.0	31.0	34.0	30.0	34.0
3	32.095131257806145	33.0	31.0	34.0	30.0	34.0
4	35.801792118802354	37.0	35.0	37.0	35.0	37.0
5	35.82525924992265	37.0	35.0	37.0	35.0	37.0
6	35.787576629120785	37.0	35.0	37.0	35.0	37.0
7	36.06913866003598	37.0	35.0	37.0	35.0	37.0
8	35.93493371222972	37.0	35.0	37.0	35.0	37.0
9	37.7785449920363	39.0	38.0	39.0	35.0	39.0
10	37.15256156111423	39.0	37.0	39.0	33.0	39.0
11	37.06781175877439	39.0	37.0	39.0	33.0	39.0
12	37.24332939922769	39.0	37.0	39.0	34.0	39.0
13	37.29724192457976	39.0	37.0	39.0	34.0	39.0
14	38.47677464449817	40.0	38.0	41.0	34.0	41.0
15	38.56690538666911	40.0	38.0	41.0	34.0	41.0
16	38.454645873199574	40.0	38.0	41.0	34.0	41.0
17	38.440095793562584	40.0	38.0	41.0	34.0	41.0
18	38.20203504027684	39.0	38.0	41.0	34.0	41.0
19	37.94233823377754	39.0	37.0	41.0	34.0	41.0
20	37.84182145271625	39.0	35.0	41.0	34.0	41.0
21	37.78699682597885	39.0	35.0	41.0	34.0	41.0
22	37.88009075179613	39.0	35.0	41.0	34.0	41.0
23	37.8816697413803	39.0	35.0	41.0	34.0	41.0
24	37.84169082513091	40.0	35.0	41.0	34.0	41.0
25	37.59997249945572	39.0	35.0	41.0	33.0	41.0
26	37.6786423897973	39.0	35.0	41.0	34.0	41.0
27	37.7000584386566	39.0	35.0	41.0	34.0	41.0
28	37.559624617570556	39.0	35.0	41.0	33.0	41.0
29	37.50321641782494	39.0	35.0	41.0	33.0	41.0
30	37.309605710946364	39.0	35.0	41.0	33.0	41.0
31	37.16827353874712	39.0	35.0	41.0	33.0	41.0
32	36.897757559785035	40.0	35.0	41.0	32.0	41.0
33	36.49808298289237	40.0	35.0	41.0	30.0	41.0
34	36.163696989836254	40.0	35.0	41.0	28.0	41.0
35	35.96044046705091	40.0	35.0	41.0	25.0	41.0
36	35.79423176083694	40.0	35.0	41.0	24.0	41.0
37	35.69934113279325	40.0	35.0	41.0	23.0	41.0
38	35.593230282682676	39.0	35.0	41.0	23.0	41.0
39	35.4999805204478	39.0	35.0	41.0	23.0	41.0
40	35.41594114883524	39.0	35.0	41.0	23.0	41.0
41	35.27042889390519	39.0	35.0	41.0	22.0	41.0
42	35.281660574532204	39.0	35.0	41.0	22.0	41.0
43	35.20860996207217	39.0	35.0	41.0	21.0	41.0
44	35.020155607246394	39.0	35.0	41.0	20.0	41.0
45	35.003105269791796	39.0	35.0	41.0	20.0	41.0
46	34.91719586116808	39.0	35.0	41.0	20.0	41.0
47	34.87730861339964	39.0	35.0	41.0	20.0	41.0
48	34.80404028829737	39.0	35.0	41.0	20.0	41.0
49	34.730144034100675	39.0	35.0	41.0	20.0	41.0
50	34.63087623609217	39.0	34.0	40.0	20.0	41.0
51	33.55532307410251	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	6.0
12	4.0
13	11.0
14	6.0
15	18.0
16	34.0
17	56.0
18	142.0
19	291.0
20	467.0
21	805.0
22	1127.0
23	1757.0
24	3022.0
25	5539.0
26	8673.0
27	10060.0
28	9614.0
29	8231.0
30	7978.0
31	8373.0
32	10122.0
33	13155.0
34	21020.0
35	26431.0
36	31557.0
37	44975.0
38	86916.0
39	135927.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.84522922849515	19.56205383231543	22.34212968798341	21.250587251206014
2	39.35786229102451	22.546779571679025	23.149270662648533	14.946087474647934
3	27.663485006474087	22.313024945285377	35.03913098280071	14.984359065439836
4	24.21881266400064	23.409838319716744	35.6558306883157	16.715518327966908
5	23.373629269745962	26.80569719609034	32.956881438278465	16.86379209588523
6	23.011767941240503	31.62929266308396	31.966174330533626	13.392765065141916
7	73.26351250701836	4.801595031568333	17.956938731079052	3.977953730334246
8	75.29603190063136	4.406962221127293	16.59955769957947	3.697448178661869
9	69.2280368048951	6.738550033802753	18.46111537624182	5.5722977850603295
10	32.313139530886545	31.37170423164625	24.43629613502767	11.878860102439527
11	22.04351961132564	24.017371177137882	37.13765168268955	16.801457528846925
12	20.567886239415156	21.95987212246909	38.95222926287083	18.520012375244928
13	20.418695786687444	22.063686677132154	40.07768903759554	17.439928498584866
14	17.712642229377458	26.169517938375865	37.88429145993514	18.233548372311535
15	16.92039738286487	25.772135073506664	39.99014563829909	17.31732190532938
16	19.651888943635342	26.08518293591227	37.60974435952378	16.653183760928602
17	20.059126170205452	25.14558100629075	36.264051059343885	18.531241764159915
18	20.558031877714246	24.782344650571208	36.38872019342049	18.27090327829405
19	20.120544052434372	26.584546985825764	34.97221299171546	18.32269597002441
20	20.70561813202553	26.180059813683815	35.44063892931214	17.673683124978513
21	20.828683067685716	25.50812984840325	36.65455878814268	17.008628295768354
22	19.9058106358355	23.780866496316072	36.708872363098855	19.604450504749575
23	18.660952664688153	25.58192297555889	36.62453736063526	19.13258699911769
24	18.47005305313334	25.090350746525193	37.040024750489856	19.39957144985161
25	19.431197075775458	26.928303789345833	34.62295607933907	19.01754305553964
26	18.859873268325103	26.699820100606157	34.71989549793173	19.720411133137013
27	19.4456348615233	25.896804207583273	35.10467394667186	19.552886984221562
28	18.56859667014243	26.765592235679662	35.90218973083842	18.76362136333948
29	19.436926355834125	26.023994224885698	34.99513011195013	19.543949307330042
30	20.2335254551913	25.862657698433615	34.834251927902734	19.069564918472345
31	20.73495204592591	26.871240159961502	33.15465618590368	19.239151608208914
32	20.998040586219936	27.768903759553577	32.32299389258746	18.91006176163903
33	21.024166103287463	27.17122526383335	31.681543697219006	20.123064935660185
34	20.116189799589783	28.819653722313255	31.813546309770715	19.250610168326247
35	20.854350242348545	28.183703635801127	30.926653756688932	20.035292365161396
36	21.384194062174146	29.798214756333717	29.786756196216384	19.03083498527575
37	20.999644784636363	29.74642206460336	30.275578370821922	18.978354779938353
38	21.270754317012525	28.873738126067078	30.458456990294604	19.397050566625797
39	20.980165232436892	28.054909420082275	30.261598927478772	20.703326420002064
40	22.349921508863197	27.162287586941826	31.609813110884488	18.877977793310492
41	20.245671528915675	28.576503076623393	31.3366410376872	19.841184356773727
42	21.026916157715622	28.631733336388947	30.967675401908995	19.373675103986432
43	21.0003322982434	27.820008937676892	30.970654627539503	20.2090041365402
44	21.179315007276188	27.584879284069164	30.675711290119285	20.560094418535364
45	20.609824569444605	26.4527735444764	31.005488650296204	21.93191323578279
46	21.051208305164373	27.37037503867264	30.44333169093972	21.135084965223268
47	20.21748347102703	27.553253658145316	31.252076864021266	20.977186006806384
48	20.133377639765786	26.842135417263467	31.92102760367132	21.103459339299423
49	20.429696004400085	26.248352831983134	31.859609721442407	21.462341442174377
50	19.54807438897228	26.032702730574876	33.1115719998625	21.307650880590344
51	19.519198817476596	25.96693059550137	31.793837586368895	22.72003300065314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	360.5
2	437.0
3	7568.0
4	14699.0
5	9823.0
6	4947.0
7	4780.5
8	4614.0
9	4632.5
10	4651.0
11	4594.5
12	4538.0
13	4304.0
14	4070.0
15	3923.0
16	3776.0
17	3514.5
18	3253.0
19	3151.0
20	3049.0
21	2988.0
22	2927.0
23	3049.0
24	3171.0
25	3580.0
26	4455.0
27	4921.0
28	6216.0
29	7511.0
30	8077.0
31	8643.0
32	9943.5
33	11244.0
34	12449.0
35	13654.0
36	15059.0
37	16464.0
38	18225.5
39	19987.0
40	20933.0
41	21879.0
42	23355.5
43	24832.0
44	26971.5
45	29111.0
46	34100.0
47	39089.0
48	39236.5
49	39384.0
50	37204.5
51	35025.0
52	30771.5
53	26518.0
54	23378.5
55	20239.0
56	17895.0
57	15551.0
58	14166.5
59	12782.0
60	11487.5
61	10193.0
62	8817.5
63	7442.0
64	6504.5
65	5567.0
66	4289.5
67	3012.0
68	2443.5
69	1875.0
70	1512.5
71	1150.0
72	943.5
73	737.0
74	646.5
75	432.5
76	309.0
77	222.0
78	135.0
79	101.5
80	68.0
81	50.5
82	33.0
83	28.0
84	23.0
85	16.0
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	436355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.00033068933146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89289315818431	30.914181800147
2	9.167064805241042	7.8837363615179346
3	4.4498156017092	5.740306271401262
4	3.1331372793951333	5.389037564362521
5	2.2722374423838687	4.885348071359355
6	1.7322821789519809	4.46932239253025
7	1.4471771498611845	4.356036720705259
8	1.1485747615383826	3.9511275654056384
9	0.9194664165260257	3.558362397151832
>10	3.7838383118057095	22.781392080409766
>50	0.031037478753798413	0.9264116497948696
>100	0.01658899726496031	1.256615504105705
>500	0.0042810315522478215	1.275484295780612
>1k	0.0010702578880619554	0.6093239067701716
>5k	5.351289440309777E-4	2.0033134185578225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8706	1.9951644876304842	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	1564	0.3584237604702593	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	1084	0.24842158334383702	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	861	0.19731640522051996	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	809	0.1853995026984909	No Hit
CGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	778	0.1782951954257428	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	750	0.17187840176003483	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	692	0.15858647202392548	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	624	0.14300283026434898	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	526	0.1205440524343711	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	503	0.11527311478039669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.875136070401393E-4	0.0	0.0	0.09831444580673993	0.0
2	6.875136070401393E-4	0.0	0.0	0.4054038569513355	0.0
3	6.875136070401393E-4	0.0	0.0	0.7851405392398392	0.0
4	6.875136070401393E-4	0.0	0.0	1.3186510983029873	0.0
5	6.875136070401393E-4	0.0	0.0	2.4720697597139942	0.0
6	6.875136070401393E-4	0.0	0.0	3.6823228793069864	0.0
7	6.875136070401393E-4	0.0	0.0	4.55569433145031	0.0
8	6.875136070401393E-4	0.0	0.0	5.66007035555912	0.0
9	6.875136070401393E-4	0.0	0.0	6.308166515795625	0.0
10	6.875136070401393E-4	0.0	0.0	7.25464358148755	0.0
11	6.875136070401393E-4	0.0	0.0	7.951553207823905	0.0
12	6.875136070401393E-4	0.0	0.0	8.73417286383793	0.0
13	6.875136070401393E-4	0.0	0.0	9.132472413516517	0.0
14	6.875136070401393E-4	0.0	0.0	9.359122732637417	0.0
15	6.875136070401393E-4	0.0	0.0	9.580960456509034	0.0
16	9.166848093868524E-4	0.0	0.0	9.892174949295871	0.0
17	9.166848093868524E-4	0.0	0.0	10.245556943314503	0.0
18	9.166848093868524E-4	0.0	0.0	10.686940679034272	0.0
19	9.166848093868524E-4	0.0	0.0	10.989446666131935	0.0
20	9.166848093868524E-4	0.0	0.0	11.275910669065325	0.0
21	9.166848093868524E-4	0.0	0.0	11.601333776397658	0.0
22	0.0011458560117335655	0.0	0.0	11.952882400797515	0.0
23	0.0011458560117335655	0.0	0.0	12.278076336927501	0.0
24	0.0011458560117335655	0.0	0.0	12.558811059802226	0.0
25	0.0011458560117335655	0.0	0.0	12.809753526371876	0.0
26	0.0011458560117335655	0.0	0.0	13.068946156226009	0.0
27	0.0013750272140802787	0.0	0.0	13.349680879100733	0.0
28	0.0013750272140802787	0.0	0.0	13.63774908045055	0.0
29	0.0013750272140802787	0.0	0.0	13.943463464381066	0.0
30	0.0013750272140802787	0.0	0.0	14.336262905203332	0.0
31	0.0013750272140802787	0.0	0.0	14.634872981861099	0.0
32	0.0013750272140802787	0.0	0.0	14.954337637932417	0.0
33	0.0013750272140802787	0.0	0.0	15.256614453827732	0.0
34	0.0013750272140802787	0.0	0.0	15.576308281101397	0.0
35	0.0013750272140802787	0.0	0.0	15.895085423565675	0.0
36	0.0013750272140802787	0.0	0.0	16.212945881220566	0.0
37	0.0013750272140802787	0.0	0.0	16.529660482863722	0.0
38	0.0013750272140802787	0.0	0.0	16.864250438289925	0.0
39	0.0013750272140802787	0.0	0.0	17.215111549082742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	45	3.8380676E-10	45.000004	9
CTCGATC	25	3.8868136E-5	45.000004	16
GGCGCGA	45	3.8380676E-10	45.000004	8
TAGTGCG	25	3.8868136E-5	45.000004	1
GTCTACT	20	7.0284726E-4	45.0	29
AAGCGCG	20	7.0284726E-4	45.0	1
CGTAAGG	70	0.0	45.0	2
CGTTTTT	4900	0.0	44.21939	1
CGCAAGG	60	3.6379788E-12	41.249996	2
CGTGAAT	50	1.0786607E-9	40.500004	27
TGGGCCG	40	3.4516415E-7	39.375	6
GCGAAAG	35	6.2393847E-6	38.571426	1
GGACGTT	35	6.2393847E-6	38.571426	8
GCGTAAG	35	6.2393847E-6	38.571426	1
GTTTTTT	5660	0.0	38.440815	2
CCCCCGT	65	9.094947E-12	38.076927	23
ACGCCGG	90	0.0	37.500004	27
CACGCCG	90	0.0	37.500004	26
GCGCGAG	30	1.1387619E-4	37.499996	1
CCCGTGA	60	1.546141E-10	37.499996	25
>>END_MODULE