##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554467
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79741265034709	33.0	31.0	34.0	30.0	34.0
2	31.86494777867754	33.0	31.0	34.0	30.0	34.0
3	31.93456598859806	33.0	31.0	34.0	30.0	34.0
4	35.676983481433524	37.0	35.0	37.0	33.0	37.0
5	35.68871727262398	37.0	35.0	37.0	33.0	37.0
6	35.635909801665385	37.0	35.0	37.0	33.0	37.0
7	35.91847305610614	37.0	35.0	37.0	35.0	37.0
8	35.730732397058794	37.0	35.0	37.0	35.0	37.0
9	37.576634858341436	39.0	37.0	39.0	35.0	39.0
10	36.9374191791396	39.0	37.0	39.0	32.0	39.0
11	36.90687633348784	39.0	37.0	39.0	33.0	39.0
12	37.02559935938478	39.0	37.0	39.0	33.0	39.0
13	36.985454499546414	39.0	37.0	39.0	33.0	39.0
14	38.11966627409747	40.0	37.0	41.0	33.0	41.0
15	38.180490452993595	40.0	37.0	41.0	33.0	41.0
16	38.14448831039539	40.0	37.0	41.0	33.0	41.0
17	38.08578328376621	40.0	37.0	41.0	33.0	41.0
18	37.9275556525456	39.0	37.0	41.0	33.0	41.0
19	37.686044435466854	39.0	37.0	41.0	33.0	41.0
20	37.57653025337847	39.0	35.0	41.0	33.0	41.0
21	37.58967981863664	39.0	35.0	41.0	33.0	41.0
22	37.70459919165613	39.0	35.0	41.0	33.0	41.0
23	37.69096086872618	39.0	35.0	41.0	33.0	41.0
24	37.674692993451366	39.0	35.0	41.0	33.0	41.0
25	37.446064418621845	39.0	35.0	41.0	33.0	41.0
26	37.48547524018562	39.0	35.0	41.0	33.0	41.0
27	37.4897207588549	39.0	35.0	41.0	33.0	41.0
28	37.35783554296288	39.0	35.0	41.0	33.0	41.0
29	37.28536053543313	39.0	35.0	41.0	33.0	41.0
30	37.13370678507467	39.0	35.0	41.0	32.0	41.0
31	37.03247984100046	39.0	35.0	41.0	32.0	41.0
32	36.76687160822917	39.0	35.0	41.0	31.0	41.0
33	36.39560334519457	39.0	35.0	41.0	30.0	41.0
34	36.08965186386205	39.0	35.0	41.0	29.0	41.0
35	35.92188353860554	39.0	35.0	41.0	27.0	41.0
36	35.75563198531202	39.0	35.0	41.0	25.0	41.0
37	35.63626509783269	39.0	35.0	41.0	25.0	41.0
38	35.52264787624872	39.0	35.0	41.0	24.0	41.0
39	35.4207518211183	39.0	35.0	41.0	23.0	41.0
40	35.31236124061486	39.0	35.0	41.0	23.0	41.0
41	35.187410612353844	39.0	35.0	41.0	23.0	41.0
42	35.24050484519367	39.0	35.0	41.0	23.0	41.0
43	35.16152990168937	39.0	35.0	41.0	23.0	41.0
44	34.98025851854123	39.0	35.0	41.0	22.0	41.0
45	34.9972423967522	39.0	35.0	40.0	22.0	41.0
46	34.875803248885866	39.0	34.0	40.0	22.0	41.0
47	34.777330300991764	38.0	34.0	40.0	21.0	41.0
48	34.737434328823895	38.0	34.0	40.0	21.0	41.0
49	34.633552582930996	38.0	34.0	40.0	20.0	41.0
50	34.479905927674686	38.0	34.0	40.0	20.0	41.0
51	33.300856498222615	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	6.0
13	3.0
14	11.0
15	18.0
16	34.0
17	69.0
18	165.0
19	399.0
20	761.0
21	1276.0
22	1850.0
23	2697.0
24	4030.0
25	6668.0
26	10313.0
27	11907.0
28	11151.0
29	10195.0
30	10705.0
31	12163.0
32	15021.0
33	19583.0
34	29924.0
35	39124.0
36	42364.0
37	61307.0
38	110955.0
39	151695.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19084995139476	19.41558289312078	23.379750282703927	17.013816872780527
2	35.02480760802717	27.122083009448716	22.80279980593976	15.05030957658436
3	26.554510908674455	26.384798373933886	32.53106136163198	14.529629355759678
4	23.857867104805155	22.951952054856285	36.976231227467096	16.213949612871463
5	25.546696196527474	26.18514717737936	31.212317414742447	17.055839211350722
6	22.251098802994587	34.01428759511387	30.518497944873186	13.216115657018362
7	72.73471640332066	4.6383283405504745	18.571529054028463	4.055426202100396
8	74.16293485455401	6.951180142370962	14.79294529701497	4.092939706060053
9	68.52887547861279	6.659368366377079	15.9829169274276	8.828839227582526
10	33.275019072370405	30.68225881792785	22.881614234931927	13.161107874769822
11	24.393155949767976	24.2907152274166	33.726804300346096	17.589324522469326
12	22.136213697118134	21.584873400941806	36.78217098582963	19.496741916110427
13	21.49704130272857	23.138257100963628	38.9736449599345	16.39105663637331
14	18.506782189021166	28.86862518418589	33.725000766501886	18.899591860291054
15	16.51694329869947	25.611984121688035	40.150991853437624	17.720080726174867
16	18.89147595799209	26.044110830761795	34.7625737870784	20.301839424167714
17	19.43415929171619	25.361292917342244	34.05721170060617	21.147336090335404
18	20.22464817563534	25.253982653611484	34.42188624390631	20.099482926846864
19	20.141144558648215	27.371872446872402	32.51194390288331	19.975039091596074
20	21.651243446408895	25.948703890402857	34.331709551695596	18.06834311149266
21	20.351977665036873	28.117272984686192	34.07668986612368	17.45405948415325
22	19.243706117767154	23.72548772063982	34.40601514607722	22.624791015515804
23	18.311098766923912	27.844578667440985	33.8732512484963	19.971071317138804
24	19.443357314321684	25.606393166770967	33.904091677232365	21.04615784167498
25	18.251401796680415	29.713580790200318	31.91262960645088	20.122387806668385
26	18.01081038186222	27.714183170504285	33.11901339484586	21.155993052787633
27	19.857268331568875	28.206728263359228	32.05384630645287	19.882157098619036
28	17.529627552225833	27.83574135160433	34.8727697049599	19.761861391209937
29	18.693989002050618	27.053007663215308	32.90295004030898	21.3500532944251
30	18.992293499883672	29.79149345227038	32.448820218335804	18.767392829510143
31	19.742202872308003	30.07284473196782	29.399044487769338	20.785907907954847
32	20.557219816508468	29.663081842562317	29.058356944597243	20.721341396331976
33	21.423817828653462	27.938723134108972	28.940766537954467	21.696692499283095
34	19.398088614831902	30.381790079481735	29.01344895187631	21.206672353810056
35	18.68028214483459	29.322033592621384	28.281935624662964	23.715748637881067
36	21.304784594935317	30.581801982805107	28.00473247280722	20.108680949452356
37	19.450391096314117	30.210274010896953	29.701677466828503	20.637657425960427
38	20.838571096205904	28.815961995934835	27.981647239601276	22.363819668257985
39	21.865683620485978	27.802917035639634	28.092384217636035	22.23901512623835
40	21.938005327638976	26.295884155414118	30.28530101881627	21.480809498130636
41	20.56353218496322	28.80946927409566	28.49403120474257	22.132967336198547
42	20.20048082212287	27.87397626910168	30.02396896478961	21.90157394398585
43	21.86315867310408	27.479002357218736	28.35660192581344	22.301237043863747
44	20.805927133625627	27.682080268077268	28.899104906153116	22.61288769214399
45	19.85023454957644	26.65063926257108	28.534791069621818	24.96433511823066
46	22.093469945010252	28.253980850077642	27.8245594417702	21.8279897631419
47	18.946483740240627	27.162482167559116	32.19470229968601	21.696331792514254
48	20.039785956603367	26.824499925153344	29.341331404754477	23.794382713488808
49	19.983696054048302	25.08877895348145	31.771232553064475	23.15629243940577
50	19.730119195551765	25.254523713764748	31.055951030449062	23.95940606023442
51	19.78963581241084	25.167232675704774	28.668793634246942	26.374337877637444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	390.5
2	525.0
3	6898.5
4	13272.0
5	9079.5
6	4887.0
7	4698.0
8	4509.0
9	4528.5
10	4548.0
11	4497.0
12	4446.0
13	4368.5
14	4291.0
15	4063.5
16	3836.0
17	3578.0
18	3320.0
19	3335.0
20	3350.0
21	3262.0
22	3174.0
23	3183.0
24	3192.0
25	3513.5
26	4491.0
27	5147.0
28	6006.5
29	6866.0
30	8325.0
31	9784.0
32	10871.5
33	11959.0
34	13639.0
35	15319.0
36	17396.0
37	19473.0
38	21554.0
39	23635.0
40	25848.5
41	28062.0
42	30984.5
43	33907.0
44	36671.5
45	39436.0
46	55676.0
47	71916.0
48	64006.0
49	56096.0
50	52727.5
51	49359.0
52	42021.0
53	34683.0
54	29686.0
55	24689.0
56	22061.0
57	19433.0
58	17292.5
59	15152.0
60	13486.0
61	11820.0
62	10217.5
63	8615.0
64	6811.0
65	5007.0
66	3873.0
67	2739.0
68	2115.5
69	1492.0
70	1238.0
71	984.0
72	785.0
73	586.0
74	458.5
75	256.0
76	181.0
77	182.0
78	183.0
79	139.5
80	96.0
81	61.5
82	27.0
83	27.0
84	27.0
85	23.0
86	19.0
87	10.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.408405937094365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9777414060439	31.10547822856099
2	8.681997589068585	7.016513658479159
3	3.380904947285843	4.098509386339709
4	1.9090614183923396	3.0856851501297125
5	1.3372207249976755	2.701747894160085
6	1.0507569727211823	2.5475648576969956
7	0.8081163133880137	2.2858284425039717
8	0.667695179970847	2.158439829960261
9	0.5842862932710979	2.1249069949780823
>10	4.513182554149378	32.531120642783634
>50	0.060849134155030696	1.6663905741206952
>100	0.02013390468293022	1.3838074703228154
>500	0.003579360832520928	1.0994164132522917
>1k	0.002684520624390696	1.6329434376738525
>5k	0.001789680416260464	4.5616470190377525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8145	1.468978316112591	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	6339	1.1432601038474788	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	5641	1.0173734415213167	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	5106	0.9208843808558489	No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	2389	0.43086423538280905	No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	1655	0.29848485121747553	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTT	1464	0.26403735479298135	No Hit
CGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTG	1419	0.25592145249401677	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT	1104	0.19911013640126463	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	1001	0.1805337378058568	No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	899	0.16213769259487038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTC	849	0.15312002337379862	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTT	843	0.15203790306727002	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	760	0.13706857216029086	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT	749	0.1350846849316551	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTA	697	0.12570630894174042	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	646	0.11650828633624724	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT	638	0.11506545926087575	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19766730932589316	0.0
2	0.0	0.0	0.0	0.9850901857098799	0.0
3	0.0	0.0	0.0	1.5081150005320425	0.0
4	0.0	0.0	0.0	2.2756990046296712	0.0
5	0.0	0.0	0.0	4.483224429948041	0.0
6	0.0	0.0	0.0	5.657685669300427	0.0
7	0.0	0.0	0.0	6.604721290897384	0.0
8	0.0	0.0	0.0	7.880360778910196	0.0
9	0.0	0.0	0.0	8.375070112378193	0.0
10	0.0	0.0	0.0	10.32469019797391	0.0
11	0.0	0.0	0.0	11.442520474617966	0.0
12	0.0	0.0	0.0	13.280862522025657	0.0
13	0.0	0.0	0.0	13.79649284808654	0.0
14	0.0	0.0	0.0	14.053676774271507	0.0
15	0.0	0.0	0.0	14.438190189858007	0.0
16	0.0	0.0	0.0	14.998728508639829	0.0
17	0.0	0.0	0.0	15.736554204307922	0.0
18	0.0	0.0	0.0	16.579886629862553	0.0
19	0.0	0.0	0.0	17.194891670739647	0.0
20	0.0	0.0	0.0	17.68779747036343	0.0
21	0.0	0.0	0.0	18.2495982628362	0.0
22	0.0	0.0	0.0	18.8999525670599	0.0
23	0.0	0.0	0.0	19.515137961321415	0.0
24	0.0	0.0	0.0	19.990369129271894	0.0
25	0.0	0.0	0.0	20.43331704141094	0.0
26	0.0	0.0	0.0	20.881134494929366	0.0
27	0.0	0.0	0.0	21.34662658012109	0.0
28	0.0	0.0	0.0	21.788672725338028	0.0
29	0.0	0.0	0.0	22.285005239265818	0.0
30	0.0	0.0	0.0	22.861595009261148	0.0
31	0.0	0.0	0.0	23.375241448093394	0.0
32	0.0	0.0	0.0	23.841454946822804	0.0
33	1.8035338442143537E-4	0.0	0.0	24.29684724248693	0.0
34	1.8035338442143537E-4	0.0	0.0	24.762700034447498	0.0
35	1.8035338442143537E-4	0.0	0.0	25.238291909166822	0.0
36	1.8035338442143537E-4	0.0	0.0	25.70071798682338	0.0
37	1.8035338442143537E-4	0.0	0.0	26.175227741236178	0.0
38	1.8035338442143537E-4	0.0	0.0	26.643605480578646	0.0
39	1.8035338442143537E-4	0.0	0.0	27.11504922745628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTCG	30	2.1634605E-6	45.000004	40
GCTACGA	30	2.1634605E-6	45.000004	10
CCACGAA	35	1.2104101E-7	45.000004	25
TGCGTAG	30	2.1634605E-6	45.000004	1
CGGGTCC	40	6.8048394E-9	45.0	6
CGAACGG	20	7.0301234E-4	45.0	2
GACCGAC	25	3.8881844E-5	45.0	9
CCGTCGA	20	7.0301234E-4	45.0	41
CGTAGAT	20	7.0301234E-4	45.0	37
CGGGCTA	25	3.8881844E-5	45.0	6
CAAGCGT	20	7.0301234E-4	45.0	38
GATCTCG	20	7.0301234E-4	45.0	9
CGTCATA	50	2.1827873E-11	45.0	38
TCGCGTC	25	3.8881844E-5	45.0	40
CCGCAGG	40	6.8048394E-9	45.0	2
CGTTTTT	5175	0.0	44.173912	1
CGGCGAA	70	0.0	41.785717	31
GCATGCG	65	0.0	41.538464	1
AACGAGC	115	0.0	41.08696	15
TCTTGCG	55	6.002665E-11	40.909092	1
>>END_MODULE