##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935354.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445345
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86024991860243	33.0	31.0	34.0	30.0	34.0
2	31.884390753236254	33.0	31.0	34.0	30.0	34.0
3	31.9510222411838	33.0	31.0	34.0	30.0	34.0
4	35.68238781169655	37.0	35.0	37.0	33.0	37.0
5	35.69731107343745	37.0	35.0	37.0	35.0	37.0
6	35.660160100596165	37.0	35.0	37.0	33.0	37.0
7	35.88953507954507	37.0	35.0	37.0	35.0	37.0
8	35.78286047895452	37.0	35.0	37.0	35.0	37.0
9	37.556846938890075	39.0	37.0	39.0	35.0	39.0
10	36.89323558140318	39.0	37.0	39.0	32.0	39.0
11	36.916377190717306	39.0	37.0	39.0	33.0	39.0
12	36.887970000785906	39.0	35.0	39.0	33.0	39.0
13	36.85115584546812	39.0	37.0	39.0	33.0	39.0
14	37.79751653212678	40.0	37.0	41.0	33.0	41.0
15	37.95530431463248	40.0	37.0	41.0	33.0	41.0
16	37.91121265535708	40.0	37.0	41.0	33.0	41.0
17	37.91096565583985	40.0	36.0	41.0	33.0	41.0
18	37.8258967766563	39.0	36.0	41.0	33.0	41.0
19	37.73241419573589	39.0	36.0	41.0	33.0	41.0
20	37.74650664091884	39.0	36.0	41.0	33.0	41.0
21	37.68316473745074	39.0	36.0	41.0	33.0	41.0
22	37.78057011979477	39.0	36.0	41.0	33.0	41.0
23	37.810854506057105	39.0	36.0	41.0	33.0	41.0
24	37.837234054497074	40.0	36.0	41.0	33.0	41.0
25	37.54077176121883	39.0	35.0	41.0	33.0	41.0
26	37.64779665203382	39.0	35.0	41.0	33.0	41.0
27	37.63810978005816	39.0	36.0	41.0	33.0	41.0
28	37.38694270733925	39.0	35.0	41.0	33.0	41.0
29	37.21486488003683	39.0	35.0	41.0	32.0	41.0
30	37.100566976164544	39.0	35.0	41.0	32.0	41.0
31	37.13288798571894	39.0	35.0	41.0	32.0	41.0
32	37.154406134569825	39.0	35.0	41.0	32.0	41.0
33	37.01326387407516	39.0	35.0	41.0	31.0	41.0
34	36.919017840101496	40.0	35.0	41.0	31.0	41.0
35	36.86516745444543	40.0	35.0	41.0	31.0	41.0
36	36.74214148581437	39.0	35.0	41.0	31.0	41.0
37	36.68700670266872	39.0	35.0	41.0	31.0	41.0
38	36.52747869629164	39.0	35.0	41.0	30.0	41.0
39	36.44864318674286	39.0	35.0	41.0	30.0	41.0
40	36.34486970775466	39.0	35.0	41.0	30.0	41.0
41	36.15971886964039	39.0	35.0	41.0	29.0	41.0
42	36.19253387822924	39.0	35.0	41.0	30.0	41.0
43	36.068733229294146	39.0	35.0	41.0	29.0	41.0
44	35.94767202955013	39.0	35.0	41.0	29.0	41.0
45	35.93450695528186	39.0	35.0	41.0	29.0	41.0
46	35.79944761926147	38.0	35.0	40.0	29.0	41.0
47	35.78494650215002	38.0	35.0	40.0	29.0	41.0
48	35.68335560071405	38.0	35.0	40.0	28.0	41.0
49	35.473215147806755	38.0	35.0	40.0	27.0	41.0
50	35.30164703768988	38.0	35.0	40.0	27.0	41.0
51	34.05248515196084	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	4.0
12	4.0
13	12.0
14	12.0
15	14.0
16	42.0
17	61.0
18	122.0
19	274.0
20	525.0
21	797.0
22	1264.0
23	1752.0
24	2460.0
25	3499.0
26	4841.0
27	5631.0
28	6133.0
29	6721.0
30	7788.0
31	9531.0
32	12000.0
33	17000.0
34	28903.0
35	33209.0
36	34560.0
37	51177.0
38	90904.0
39	126058.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07916334527164	20.950049961265986	25.11064455646746	12.860142136994915
2	32.995542781439106	25.69940158753326	24.57375742401958	16.73129820700805
3	30.62973649642412	25.20450437301418	28.20599759736833	15.959761533193367
4	27.48588173214025	25.310714165422315	28.869977208680908	18.333426893756528
5	25.480021107231472	29.271912786715916	26.694360551931652	18.55370555412096
6	25.297241464482596	34.37941371296411	26.000740998551684	14.322603824001618
7	79.083182701052	5.596110880328734	10.839012451021118	4.481693967598154
8	80.81083205155554	5.892510301002594	9.147739393054822	4.148918254387048
9	74.86196095162178	7.313655705127485	11.069395637090345	6.754987706160392
10	38.345776869617936	30.308637124027438	17.56166567492618	13.783920331428442
11	28.523953339545745	26.33127126160617	26.74376045537729	18.40101494347079
12	25.156002649631187	21.790746499904568	32.91493112081644	20.1383197296478
13	25.736676060133156	22.546789567638573	33.024059998428186	18.69247437380009
14	20.369152005748354	28.011317068789367	32.11824540524762	19.501285520214665
15	18.89995396827179	24.654369084642244	36.41513882495593	20.030538122130036
16	23.063692193692532	26.19542152713065	29.884022499410566	20.85686377976625
17	23.624605642816242	23.670637371026956	28.504193378167486	24.200563607989313
18	26.49945547833702	22.88966980655447	29.582009453345158	21.02886526176335
19	26.085169924440603	25.11064455646746	27.71940854842875	21.084776970663192
20	24.63842638853024	25.95111655009038	28.192749441444278	21.217707619935105
21	25.351356813257137	26.207996048007725	29.781629972268693	18.659017166466445
22	23.09602667594786	24.809080600433372	29.401699805768562	22.693192917850205
23	21.34749463898775	25.987268297611966	29.302226363830293	23.363010699569998
24	22.39971258237995	24.710056248526424	29.11809945098744	23.772131718106188
25	22.19919388339377	26.506640918838205	28.067004232673543	23.227160965094477
26	20.365110195466436	25.390652190997994	28.855830872694206	25.38840674084137
27	21.678249447057897	25.074043718914552	29.212857447596807	24.034849386430746
28	21.53880699233179	25.331147761847557	30.321660734935836	22.808384510884817
29	21.87562451582481	25.57882091412276	30.818578854595874	21.726975715456557
30	23.571388474104346	26.07686175886111	29.902210645679194	20.449539121355354
31	23.985449482985103	27.64441051319763	27.55481705194849	20.815322951868776
32	24.754965251658824	26.911495582076817	27.732656704352802	20.600882461911553
33	24.3153061109926	26.31375675038453	27.711549472880577	21.659387665742287
34	22.93031245438929	25.724101539256083	29.97159505551876	21.373990950835868
35	21.663204931008543	27.764093006545487	28.468939810708555	22.103762251737415
36	23.831860692272283	27.675846815390315	27.734453064478103	20.757839427859302
37	23.134199328610404	28.101359620069832	27.297713009015485	21.466728042304283
38	23.86397062951195	27.78699659814301	25.631588992803334	22.717443779541703
39	25.683234346405598	24.5582638179389	26.602521640525882	23.15598019512962
40	24.720385319246876	24.211341768741086	28.24911024037544	22.819162671636597
41	22.222771110038284	24.867911394536822	28.01558342408694	24.89373407133795
42	23.70005276807868	24.763947052285307	28.56257508223961	22.9734250973964
43	23.506719509593683	25.354051353445083	28.15569951386004	22.98352962310119
44	23.119828447608036	24.501229383960748	28.03646611054351	24.342476057887705
45	22.321795461945236	23.86688971471556	27.855482827919925	25.95583199541928
46	22.418574363695562	25.551875512243317	27.575699738405056	24.453850385656065
47	20.742570366794283	25.28825966385611	29.662845658983482	24.30632431036612
48	21.9259225993331	25.08302551954103	28.933523448113263	24.05752843301261
49	24.197419977770043	23.72026181948826	29.538447720306728	22.543870482434965
50	21.956011631431814	24.239634440714504	30.288428072617858	23.515925855235828
51	22.412062558241363	23.757311747072492	28.228003008903208	25.602622685782933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	202.0
1	386.5
2	571.0
3	2479.0
4	4387.0
5	2833.0
6	1279.0
7	1323.5
8	1368.0
9	1337.5
10	1307.0
11	1347.5
12	1388.0
13	1379.5
14	1371.0
15	1341.5
16	1312.0
17	1276.5
18	1241.0
19	1293.0
20	1345.0
21	1459.5
22	1574.0
23	1778.5
24	1983.0
25	2515.0
26	3589.5
27	4132.0
28	4189.5
29	4247.0
30	5364.5
31	6482.0
32	7383.0
33	8284.0
34	9983.5
35	11683.0
36	13142.5
37	14602.0
38	15357.5
39	16113.0
40	17372.0
41	18631.0
42	21030.0
43	23429.0
44	26057.0
45	28685.0
46	35199.0
47	41713.0
48	41490.5
49	41268.0
50	40212.0
51	39156.0
52	34503.0
53	29850.0
54	27984.0
55	26118.0
56	24862.5
57	23607.0
58	22826.5
59	22046.0
60	20884.0
61	19722.0
62	16582.0
63	13442.0
64	11928.0
65	10414.0
66	8591.5
67	6769.0
68	5571.0
69	4373.0
70	3756.5
71	3140.0
72	2660.0
73	2180.0
74	1824.0
75	1076.5
76	685.0
77	516.5
78	348.0
79	294.0
80	240.0
81	151.0
82	62.0
83	58.0
84	54.0
85	34.5
86	15.0
87	10.5
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.21926670031299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12543242832373	27.96113505526123
2	8.354642125174799	6.219073068851011
3	3.205034837988236	3.578671392566358
4	1.810870731308657	2.6959712283347095
5	1.282855976442853	2.3873479362658494
6	1.0620277661282684	2.3716736802399394
7	0.8838628099180219	2.3027707954178833
8	0.8188423370866684	2.4381369063629044
9	0.7150800746786211	2.395328041038899
>10	6.662771090426729	42.49007550094417
>50	0.05017019438029993	1.267962729480058
>100	0.022969486583751777	1.5131861814199559
>500	0.0030223008662831285	0.8283603211400947
>1k	0.002417840693026503	1.5503071626769127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2557	0.574161605047772	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	1789	0.4017110330193445	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	1296	0.29101034029797124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	1249	0.28045672456185655	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	910	0.20433596425243353	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	875	0.196476888704263	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	737	0.16548967654290495	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	582	0.13068519911529264	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	578	0.1297870190526446	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	495	0.11114978275269735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.981800626480593E-4	0.0	0.0	0.14550517014898562	0.0
2	8.981800626480593E-4	0.0	0.0	0.6626323412186058	0.0
3	8.981800626480593E-4	0.0	0.0	1.0052880351188405	0.0
4	8.981800626480593E-4	0.0	0.0	1.4568480616151522	0.0
5	8.981800626480593E-4	0.0	0.0	2.6810674870044573	0.0
6	0.0011227250783100741	0.0	0.0	3.568244843885078	0.0
7	0.0011227250783100741	0.0	0.0	4.180579101595392	0.0
8	0.0011227250783100741	0.0	0.0	5.057427387755561	0.0
9	0.0011227250783100741	0.0	0.0	5.4497075301171005	0.0
10	0.0011227250783100741	0.0	0.0	6.490249132694877	0.0
11	0.0011227250783100741	0.0	0.0	7.510806228878734	0.0
12	0.0011227250783100741	0.0	0.0	8.68495211577541	0.0
13	0.0011227250783100741	0.0	0.0	9.197139296500467	0.0
14	0.0011227250783100741	0.0	0.0	9.47175785065511	0.0
15	0.0011227250783100741	0.0	0.0	9.772872716657872	0.0
16	0.0011227250783100741	0.0	0.0	10.292918972931098	0.0
17	0.0011227250783100741	0.0	0.0	10.900313240296848	0.0
18	0.001347270093972089	0.0	0.0	11.690936240442802	0.0
19	0.001347270093972089	0.0	0.0	12.155968967878836	0.0
20	0.001347270093972089	0.0	0.0	12.611570804657063	0.0
21	0.001347270093972089	0.0	0.0	13.117470724943583	0.0
22	0.001347270093972089	0.0	0.0	13.635496076075851	0.0
23	0.001571815109634104	0.0	0.0	14.178445923946603	0.0
24	0.001571815109634104	0.0	0.0	14.57589060166837	0.0
25	0.0017963601252961186	0.0	0.0	14.963230753685345	0.0
26	0.0017963601252961186	0.0	0.0	15.345406370342094	0.0
27	0.0020209051409581335	0.0	0.0	15.769122814896317	0.0
28	0.0020209051409581335	0.0	0.0	16.140969360832614	0.0
29	0.0020209051409581335	0.0	0.0	16.548294019243507	0.0
30	0.0020209051409581335	0.0	0.0	17.075525716017918	0.0
31	0.0020209051409581335	0.0	0.0	17.49452671524324	0.0
32	0.0020209051409581335	0.0	0.0	17.909934994217966	0.0
33	0.0020209051409581335	0.0	0.0	18.385521337390113	0.0
34	0.0020209051409581335	0.0	0.0	18.88895126250435	0.0
35	0.0020209051409581335	0.0	0.0	19.364313060660837	0.0
36	0.0022454501566201482	0.0	0.0	19.771188629040406	0.0
37	0.0024699951722821634	0.0	0.0	20.189066903187417	0.0
38	0.0024699951722821634	0.0	0.0	20.657018715827057	0.0
39	0.0024699951722821634	0.0	0.0	21.333348303001046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGT	20	7.0286257E-4	45.000004	12
CTATACT	20	7.0286257E-4	45.000004	38
TAATTCG	20	7.0286257E-4	45.000004	43
CGGTATC	20	7.0286257E-4	45.000004	18
TCATGCG	25	3.886943E-5	45.0	1
TCGACGG	30	2.1624946E-6	44.999996	2
CGTTTTT	1415	0.0	43.409897	1
ACAACGA	135	0.0	41.666668	13
CGTAAGG	65	0.0	41.53846	2
GTGACGG	40	3.4518598E-7	39.375004	2
GCGATGA	120	0.0	39.374996	9
CACAACG	145	0.0	38.793102	12
TACGGGA	105	0.0	38.57143	4
CTAGTCG	35	6.2396703E-6	38.57143	1
CGATGCA	35	6.2396703E-6	38.57143	10
CTTACGG	35	6.2396703E-6	38.57143	2
GTAGGGC	175	0.0	38.571426	4
TTATGAG	60	1.546141E-10	37.499996	1
AAGACGC	30	1.1387991E-4	37.499996	38
TGCGACG	30	1.1387991E-4	37.499996	1
>>END_MODULE