FastQCFastQC Report
Sat 14 Jan 2017
SRR2935351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957004
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC109520.5596309460788021TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT105820.5407244951977616TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC92980.4751140007889611TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC83210.425190750759835TruSeq Adapter, Index 19 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG46850.2393965469666899TruSeq Adapter, Index 13 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT30600.15636145863779533No Hit
TGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG23020.11762878358960942TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG21950.11216124238887606TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC21480.10975961214182495No Hit
TGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCTT19590.10010199263772582TruSeq Adapter, Index 19 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG19580.10005089412183112TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC253.8918137E-545.011
GCGGTTT207.0345047E-445.026
TGCGGTC502.1827873E-1145.04
CACGACG253.8918137E-545.026
TCGTGAC207.0345047E-445.018
GACGCGA207.0345047E-445.017
GCTACGA351.2125201E-745.010
CGCGTTT207.0345047E-445.039
CGCAATT207.0345047E-445.034
ATCGGCG207.0345047E-445.012
CGACTAT207.0345047E-445.020
CCGTTCG253.8918137E-545.01
GCGTAGT207.0345047E-445.044
CTAACGG302.1662872E-644.9999962
CCGACGG302.1662872E-644.9999962
TATCCGG302.1662872E-644.9999962
TACGGGA6350.043.2283484
ACGGGTA2250.043.05
TAACGGG950.042.631583
CGTATGG2650.042.452832
Produced by FastQC (version 0.11.5)