##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1957004
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26443941862153	33.0	31.0	34.0	30.0	34.0
2	32.41020355604792	34.0	31.0	34.0	30.0	34.0
3	32.46594539408198	34.0	31.0	34.0	30.0	34.0
4	35.955026152220434	37.0	35.0	37.0	35.0	37.0
5	31.626549051509347	37.0	35.0	37.0	0.0	37.0
6	33.40575798516508	37.0	35.0	37.0	17.0	37.0
7	35.251094530210466	37.0	35.0	37.0	32.0	37.0
8	35.81502899329792	37.0	35.0	37.0	35.0	37.0
9	37.8714003650478	39.0	38.0	39.0	35.0	39.0
10	37.46035674939857	39.0	37.0	39.0	35.0	39.0
11	37.35747704143681	39.0	37.0	39.0	34.0	39.0
12	37.27186147805523	39.0	37.0	39.0	34.0	39.0
13	37.271073538940136	39.0	37.0	39.0	34.0	39.0
14	38.47794332561405	40.0	38.0	41.0	34.0	41.0
15	38.534956494723566	40.0	38.0	41.0	34.0	41.0
16	38.570349881758034	40.0	38.0	41.0	34.0	41.0
17	38.57708211122716	40.0	38.0	41.0	35.0	41.0
18	38.55809850158712	40.0	38.0	41.0	35.0	41.0
19	38.568059646275636	40.0	38.0	41.0	34.0	41.0
20	38.5062508814494	40.0	38.0	41.0	34.0	41.0
21	38.45481460436463	40.0	38.0	41.0	34.0	41.0
22	38.414582698860094	40.0	38.0	41.0	34.0	41.0
23	38.34478008220729	40.0	38.0	41.0	34.0	41.0
24	38.32607700341951	40.0	37.0	41.0	34.0	41.0
25	38.235102738676055	40.0	37.0	41.0	34.0	41.0
26	38.18405072243082	40.0	37.0	41.0	34.0	41.0
27	38.14990720509513	40.0	37.0	41.0	34.0	41.0
28	38.14081524616199	40.0	37.0	41.0	34.0	41.0
29	38.088336048367815	40.0	37.0	41.0	34.0	41.0
30	38.0232447148805	40.0	37.0	41.0	34.0	41.0
31	37.83999675013439	40.0	37.0	41.0	33.0	41.0
32	37.88098440268901	40.0	37.0	41.0	33.0	41.0
33	37.876322174098775	40.0	37.0	41.0	33.0	41.0
34	37.88489395014011	40.0	37.0	41.0	34.0	41.0
35	37.84288892613403	40.0	37.0	41.0	33.0	41.0
36	37.81137238350049	40.0	37.0	41.0	33.0	41.0
37	37.79576791871657	40.0	37.0	41.0	33.0	41.0
38	37.803806226251965	40.0	37.0	41.0	33.0	41.0
39	37.7767286116942	40.0	37.0	41.0	33.0	41.0
40	37.72868118818357	40.0	37.0	41.0	33.0	41.0
41	37.7146045690249	40.0	37.0	41.0	33.0	41.0
42	37.714610700846805	40.0	37.0	41.0	33.0	41.0
43	37.67813044837926	40.0	36.0	41.0	33.0	41.0
44	37.62692871348244	40.0	36.0	41.0	33.0	41.0
45	37.63069058622261	40.0	36.0	41.0	33.0	41.0
46	37.513801198158	39.0	36.0	41.0	33.0	41.0
47	37.48990037833341	39.0	36.0	41.0	33.0	41.0
48	37.47839248156877	39.0	36.0	41.0	33.0	41.0
49	37.480125232242756	39.0	36.0	41.0	33.0	41.0
50	37.4325336074939	39.0	36.0	41.0	33.0	41.0
51	36.78260596299241	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	7.0
12	7.0
13	18.0
14	13.0
15	22.0
16	40.0
17	67.0
18	117.0
19	243.0
20	496.0
21	874.0
22	1449.0
23	2397.0
24	3776.0
25	5707.0
26	7882.0
27	9840.0
28	12616.0
29	16687.0
30	22518.0
31	30482.0
32	42712.0
33	62264.0
34	97762.0
35	142575.0
36	164579.0
37	263658.0
38	444582.0
39	623087.0
40	521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.422693055302904	4.520532405656811	60.165027766933534	12.891746772106751
2	19.17170327705002	4.058652920484578	54.5138895985905	22.255754203874904
3	19.245591731033766	3.9533388792255915	53.81843879726357	22.982630592477072
4	18.398940421174405	4.0815961541213	52.72789427103879	24.791569153665503
5	15.110239938191237	16.94084427011902	47.156520886007385	20.79239490568236
6	18.986777748027087	6.2815916574519015	56.555786293742884	18.17584430077813
7	82.40095574664129	1.8417438083928288	10.480765496646915	5.276534948318961
8	85.01055695338384	1.7111360017659647	7.502079709596915	5.7762273352532745
9	80.01061827160292	4.172091625770821	10.057158799879815	5.7601313027464425
10	36.540344322239505	22.386362010501767	22.727291308551234	18.346002358707494
11	25.656104944088003	27.491103748382734	24.971589225162543	21.88120208236672
12	20.442829958446687	23.55048839961492	33.239584589505185	22.767097052433208
13	21.022849212367476	25.693815648818298	35.070393315496545	18.21294182331768
14	20.77415273550795	27.17403745725609	31.846332455120173	20.205477352115786
15	18.41948202456408	26.996981099680937	34.0518976966833	20.531639179071686
16	18.327657991501294	25.680734428749254	32.49753194168228	23.49407563806717
17	18.727606075409145	27.52477767035734	29.076997287690777	24.670618966542733
18	20.478445624025294	25.299335106111176	32.72216101755541	21.500058252308122
19	21.16316573701433	28.650835665128945	29.553593145440683	20.632405452416037
20	22.16622960402738	27.733668403334892	29.431518790968237	20.66858320166949
21	22.779922779922778	28.32911940905588	29.155586805136828	19.735371005884506
22	21.149164743659185	25.955031262072026	27.991920302666728	24.90388369160206
23	19.149168831540457	27.910469268330573	27.15533540043863	25.785026499690346
24	20.809308514443504	26.84710915256177	28.85747806340713	23.486104269587592
25	18.85790729094064	28.246442010338253	27.405871423870366	25.489779274850743
26	18.216518719430315	28.55620121369195	29.853388138194916	23.373891928682824
27	19.441196849878693	27.065350913948055	29.81164065070894	23.681811585464313
28	17.46245792037216	25.54527226311239	32.875098875628254	24.117170940887192
29	18.00752578942097	24.576495500264688	33.01715274981554	24.398825960498804
30	19.319786776112874	27.160956237187044	30.258037285565077	23.261219701135
31	17.879932795231895	28.345879722269345	28.467800781194114	25.306386701304646
32	17.374415177485584	27.77643786113876	28.96723767554895	25.881909285826705
33	18.105277250327543	27.284563547136337	27.08941831493446	27.520740887601658
34	16.484790015758783	25.859988022507874	29.77035304986602	27.884868911867322
35	16.114172479974492	24.600562901251095	30.192988874831116	29.09227574394329
36	18.02403061005496	25.95298732143624	28.65287960576473	27.37010246274407
37	15.868388618520965	26.64700736431811	31.318331490380192	26.16627252678073
38	16.65908705347562	27.87005034225786	30.00750126213334	25.46336134213318
39	18.194086470952538	26.895346151566375	29.76841130626202	25.14215607121907
40	18.510897269499704	26.30515829298254	28.003110877647668	27.180833559870088
41	16.777022428160596	24.630302237501812	27.095090250198773	31.49758508413882
42	18.003948893308344	24.244406245465004	27.723550897187742	30.02809396403891
43	18.658009896760557	23.845071343747893	30.554868564397413	26.942050195094136
44	17.812022867607833	24.980480366928223	29.825488348516405	27.38200841694754
45	19.054023394944515	24.481043472573383	29.017518615189342	27.447414517292763
46	19.819376966015398	24.69054738774167	29.595902716601497	25.894172929641428
47	16.3167269969811	24.643741147182123	31.682255120582276	27.357276735254498
48	16.50267449632193	23.579103568515954	31.152159116690616	28.7660628184715
49	18.295721419067103	22.406137136153017	32.324563465378716	26.973577979401163
50	18.321934957721087	21.575070873641547	30.53448025655543	29.56851391208194
51	16.81744135423331	22.627700301072455	27.59401615939467	32.96084218529957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	464.5
2	743.0
3	2156.5
4	3570.0
5	2541.0
6	1512.0
7	1621.0
8	1730.0
9	2267.5
10	2805.0
11	3117.5
12	3430.0
13	3685.0
14	3940.0
15	4139.5
16	4339.0
17	4797.5
18	5256.0
19	5056.5
20	4857.0
21	4982.5
22	5108.0
23	5986.5
24	6865.0
25	7736.0
26	11577.5
27	14548.0
28	16580.5
29	18613.0
30	23980.5
31	29348.0
32	35418.5
33	41489.0
34	47893.5
35	54298.0
36	62469.0
37	70640.0
38	80097.5
39	89555.0
40	97255.5
41	104956.0
42	121326.0
43	137696.0
44	157834.5
45	177973.0
46	208318.0
47	238663.0
48	263922.0
49	289181.0
50	261636.5
51	234092.0
52	188691.5
53	143291.0
54	116723.5
55	90156.0
56	73753.0
57	57350.0
58	49188.0
59	41026.0
60	34218.5
61	27411.0
62	22470.0
63	17529.0
64	14203.5
65	10878.0
66	8371.0
67	5864.0
68	4632.5
69	3401.0
70	2947.0
71	2493.0
72	2163.5
73	1834.0
74	1288.5
75	605.5
76	468.0
77	424.5
78	381.0
79	226.5
80	72.0
81	64.5
82	57.0
83	33.5
84	10.0
85	23.0
86	36.0
87	19.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1957004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.115781200517514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.1615709580452	19.26345823896958
2	14.113609238672714	8.218575170855946
3	6.176618269757534	5.3951119830413825
4	3.3411066475588385	3.8911572047167087
5	1.9412597993290588	2.826064778531271
6	1.2939422055356353	2.2604482885494375
7	0.8676634194773134	1.7683888794036016
8	0.6827850543298787	1.590385619908177
9	0.5030151914248089	1.3181112228655052
>10	4.052099146233181	25.561471353611275
>50	0.6919794844716521	13.695918079726432
>100	0.16197737746556864	8.453260383847933
>500	0.00749891375667018	1.479613449805964
>1k	0.004124402566168599	2.1409008273984407
>5k	3.74945687833509E-4	0.9617187210732018
>10k+	3.74945687833509E-4	1.1754157976951205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	10952	0.5596309460788021	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	10582	0.5407244951977616	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	9298	0.4751140007889611	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	8321	0.425190750759835	TruSeq Adapter, Index 19 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	4685	0.2393965469666899	TruSeq Adapter, Index 13 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3060	0.15636145863779533	No Hit
TGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2302	0.11762878358960942	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2195	0.11216124238887606	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	2148	0.10975961214182495	No Hit
TGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCTT	1959	0.10010199263772582	TruSeq Adapter, Index 19 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1958	0.10005089412183112	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1098515894704354E-5	0.0	0.0	0.5564628380933304	0.0
2	5.1098515894704354E-5	0.0	0.0	2.107353894013502	0.0
3	5.1098515894704354E-5	0.0	0.0	3.016396491780293	0.0
4	5.1098515894704354E-5	0.0	0.0	4.0718874361013055	0.0
5	5.1098515894704354E-5	0.0	0.0	5.823186871360508	0.0
6	5.1098515894704354E-5	0.0	0.0	7.201671534651948	0.0
7	5.1098515894704354E-5	0.0	0.0	8.341934916842275	0.0
8	5.1098515894704354E-5	0.0	0.0	10.18623365102984	0.0
9	5.1098515894704354E-5	0.0	0.0	11.034417916366037	0.0
10	5.1098515894704354E-5	0.0	0.0	12.669263833901208	0.0
11	5.1098515894704354E-5	0.0	0.0	15.584434165694091	0.0
12	5.1098515894704354E-5	0.0	0.0	17.827505717923927	0.0
13	5.1098515894704354E-5	0.0	0.0	18.584990117547026	0.0
14	5.1098515894704354E-5	0.0	0.0	18.825919619990557	0.0
15	5.1098515894704354E-5	0.0	0.0	19.166746721008234	0.0
16	5.1098515894704354E-5	0.0	0.0	20.119018663221944	0.0
17	5.1098515894704354E-5	0.0	0.0	21.632199014411825	0.0
18	5.1098515894704354E-5	0.0	0.0	23.230305099018704	0.0
19	1.0219703178940871E-4	0.0	0.0	24.31221397605728	0.0
20	1.0219703178940871E-4	0.0	0.0	25.330811791902317	0.0
21	1.0219703178940871E-4	0.0	0.0	26.8439410445763	0.0
22	1.0219703178940871E-4	0.0	0.0	28.41803082671267	0.0
23	1.0219703178940871E-4	0.0	0.0	30.05798659583731	0.0
24	1.0219703178940871E-4	0.0	0.0	31.231974998518144	0.0
25	1.5329554768411306E-4	0.0	0.0	32.247047016766444	0.0
26	1.5329554768411306E-4	0.0	0.0	33.122620086622206	0.0
27	1.5329554768411306E-4	0.0	0.0	33.97555651393661	0.0
28	2.0439406357881742E-4	0.0	0.0	34.94545744413399	0.0
29	2.0439406357881742E-4	0.0	0.0	35.89522555906886	0.0
30	3.065910953682261E-4	0.0	0.0	36.85061451075215	0.0
31	3.065910953682261E-4	0.0	0.0	37.838144428933205	0.0
32	3.065910953682261E-4	0.0	0.0	38.73982884041116	0.0
33	3.065910953682261E-4	0.0	0.0	39.63814074984006	0.0
34	3.576896112629305E-4	0.0	0.0	40.48361679383384	0.0
35	3.576896112629305E-4	0.0	0.0	41.35075860856697	0.0
36	3.576896112629305E-4	0.0	0.0	42.220148757999475	0.0
37	3.576896112629305E-4	0.0	0.0	43.07742855916493	0.0
38	3.576896112629305E-4	0.0	0.0	43.95806038209426	0.0
39	3.576896112629305E-4	0.0	0.0	44.788155772803734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAC	25	3.8918137E-5	45.0	11
GCGGTTT	20	7.0345047E-4	45.0	26
TGCGGTC	50	2.1827873E-11	45.0	4
CACGACG	25	3.8918137E-5	45.0	26
TCGTGAC	20	7.0345047E-4	45.0	18
GACGCGA	20	7.0345047E-4	45.0	17
GCTACGA	35	1.2125201E-7	45.0	10
CGCGTTT	20	7.0345047E-4	45.0	39
CGCAATT	20	7.0345047E-4	45.0	34
ATCGGCG	20	7.0345047E-4	45.0	12
CGACTAT	20	7.0345047E-4	45.0	20
CCGTTCG	25	3.8918137E-5	45.0	1
GCGTAGT	20	7.0345047E-4	45.0	44
CTAACGG	30	2.1662872E-6	44.999996	2
CCGACGG	30	2.1662872E-6	44.999996	2
TATCCGG	30	2.1662872E-6	44.999996	2
TACGGGA	635	0.0	43.228348	4
ACGGGTA	225	0.0	43.0	5
TAACGGG	95	0.0	42.63158	3
CGTATGG	265	0.0	42.45283	2
>>END_MODULE