##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2266288
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.445530753372914	33.0	31.0	34.0	31.0	34.0
2	32.58706219156612	34.0	31.0	34.0	31.0	34.0
3	32.64523529224882	34.0	31.0	34.0	31.0	34.0
4	36.062998171459235	37.0	35.0	37.0	35.0	37.0
5	31.69502463941035	37.0	35.0	37.0	0.0	37.0
6	33.45867956764542	37.0	35.0	37.0	17.0	37.0
7	35.295739111710425	37.0	35.0	37.0	32.0	37.0
8	35.850306315878655	37.0	35.0	37.0	35.0	37.0
9	37.89360046031219	39.0	38.0	39.0	35.0	39.0
10	37.60159873767147	39.0	37.0	39.0	35.0	39.0
11	37.51842528398862	39.0	37.0	39.0	35.0	39.0
12	37.37225807134839	39.0	37.0	39.0	35.0	39.0
13	37.36795764704221	39.0	37.0	39.0	35.0	39.0
14	38.61976412530093	40.0	38.0	41.0	35.0	41.0
15	38.66640868239165	40.0	38.0	41.0	35.0	41.0
16	38.702081553624254	40.0	38.0	41.0	35.0	41.0
17	38.697086160276186	40.0	38.0	41.0	35.0	41.0
18	38.67448400203328	40.0	38.0	41.0	35.0	41.0
19	38.707874727307384	40.0	38.0	41.0	35.0	41.0
20	38.64996064048347	40.0	38.0	41.0	35.0	41.0
21	38.57917043200158	40.0	38.0	41.0	34.0	41.0
22	38.56013842900814	40.0	38.0	41.0	34.0	41.0
23	38.52550293696123	40.0	38.0	41.0	34.0	41.0
24	38.50929449390369	40.0	38.0	41.0	34.0	41.0
25	38.429987274344654	40.0	38.0	41.0	34.0	41.0
26	38.4049961875984	40.0	38.0	41.0	34.0	41.0
27	38.36843331474199	40.0	38.0	41.0	34.0	41.0
28	38.35685358612851	40.0	38.0	41.0	34.0	41.0
29	38.30680654885875	40.0	37.0	41.0	34.0	41.0
30	38.249478442280946	40.0	37.0	41.0	34.0	41.0
31	38.05249421079757	40.0	37.0	41.0	34.0	41.0
32	38.118050750831316	40.0	37.0	41.0	34.0	41.0
33	38.11595216494991	40.0	37.0	41.0	34.0	41.0
34	38.04012508560253	40.0	37.0	41.0	34.0	41.0
35	38.001318014303564	40.0	37.0	41.0	34.0	41.0
36	38.02362674117323	40.0	37.0	41.0	34.0	41.0
37	37.999222958423644	40.0	37.0	41.0	34.0	41.0
38	37.997121725041126	40.0	37.0	41.0	34.0	41.0
39	37.983909370741934	40.0	37.0	41.0	34.0	41.0
40	37.90668661705838	40.0	37.0	41.0	34.0	41.0
41	37.86531588218267	40.0	36.0	41.0	33.0	41.0
42	37.86744888557853	40.0	36.0	41.0	33.0	41.0
43	37.82667692720431	40.0	36.0	41.0	33.0	41.0
44	37.771276201436	40.0	36.0	41.0	33.0	41.0
45	37.74847812811081	40.0	36.0	41.0	33.0	41.0
46	37.645543726128366	40.0	36.0	41.0	33.0	41.0
47	37.62274521155299	40.0	36.0	41.0	33.0	41.0
48	37.592094649929756	40.0	36.0	41.0	33.0	41.0
49	37.58417067910168	39.0	35.0	41.0	33.0	41.0
50	37.50336012016125	39.0	35.0	41.0	33.0	41.0
51	36.87730288471721	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	16.0
11	17.0
12	22.0
13	23.0
14	26.0
15	41.0
16	85.0
17	116.0
18	182.0
19	371.0
20	580.0
21	1017.0
22	1708.0
23	2611.0
24	3888.0
25	5832.0
26	8270.0
27	10671.0
28	13609.0
29	17257.0
30	23384.0
31	31749.0
32	43814.0
33	67222.0
34	115248.0
35	162904.0
36	172886.0
37	284299.0
38	479263.0
39	818157.0
40	1011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.340722803103578	4.696049222340673	56.64663979158872	15.316588182967037
2	19.389327393517505	4.565130292354723	53.84505411492273	22.200488199205044
3	20.785487104904586	4.468275876675868	52.11116151168783	22.635075506731713
4	18.503252896366217	4.987980344951745	50.933906017240524	25.574860741441512
5	15.907686931228513	17.652301913966802	44.23983183072937	22.200179324075318
6	20.966708556017593	7.023467449856328	54.06806195858602	17.941762035540055
7	83.77064168366951	2.601522842639594	8.859641846049575	4.768193627641323
8	86.67199402723749	2.5177735574648943	6.1907842251293745	4.61944819016824
9	81.04821629025083	5.27029221352273	8.57521197658903	5.106279519637398
10	39.003692381550806	24.131046010039327	19.88101247502524	16.984249133384637
11	27.771007038822958	27.66360674371483	23.791856992579937	20.773529224882274
12	23.224144504140693	24.065829232648277	30.539587201626624	22.170439061584407
13	23.26681339706163	24.110572001440243	31.98529930882571	20.637315292672422
14	21.841751798535753	25.67268590752808	31.343236164159187	21.142326129776976
15	20.272048389260323	25.21272671434522	33.06287638640808	21.452348509986376
16	21.25859555361013	25.251512605635295	31.14105532924324	22.348836511511337
17	21.928325084896535	24.96734748628594	29.300336056141145	23.803991372676375
18	22.01586912166503	24.423947883058112	31.179223470273858	22.380959525003
19	23.69284045099299	26.600899797377913	28.856482494722645	20.84977725690645
20	24.931341471163417	26.062133321095992	28.394802425817016	20.611722781923568
21	24.29527932901732	26.759396863946684	28.54822511525455	20.39709869178145
22	23.333618675119844	25.143626935323315	28.375034417514456	23.14771997204239
23	22.24986409494292	25.86167336190281	27.91264834831231	23.975814194841963
24	22.319625749242814	25.387020537548626	28.694631926745412	23.59872178646315
25	21.079403853349618	26.903597424510917	28.052304031967694	23.96469469017177
26	20.278976017169928	26.782650748713316	29.41113397767627	23.527239256440488
27	20.588733647268132	26.84102814823182	28.915433519482075	23.654804685017968
28	19.65151825363767	25.523940470054995	30.95352400048008	23.871017275827256
29	20.946190422399976	24.647794102073522	30.351835247770804	24.054180227755694
30	20.955147801162077	25.528882472130636	29.372436336423263	24.143533390284023
31	21.888083067994888	27.321064224846975	27.874215457170493	22.916637249987645
32	22.192280945757997	26.67719195442062	27.198396673326602	23.93213042649478
33	22.158966556765954	25.652211898928996	27.305752843416194	24.883068700888856
34	19.974072139110298	25.80867921464527	29.521623024081673	24.695625622162762
35	19.6746397633487	25.17363194792542	29.467216876231088	25.684511412494793
36	20.473876224028015	25.692895166015973	29.371465586015542	24.46176302394047
37	20.34887004652542	26.28209653848055	29.791712262519148	23.577321152474884
38	20.68827968907747	26.773649244932685	27.722337143381598	24.815733922608246
39	21.40442873986007	25.352294147963544	28.639122653431514	24.604154458744873
40	21.016481576922263	25.634959016682785	28.702133179895934	24.646426226499017
41	19.514774821205425	25.472358323390498	28.2175081013534	26.795358754050675
42	20.390832939149835	24.07972861348602	29.48204288245801	26.04739556490614
43	20.955986176514195	23.5552145181901	30.28030859272961	25.2084907125661
44	20.800489611205634	23.73912759543359	29.235913529083685	26.22446926427709
45	21.52263966450866	23.859280020897607	28.012150265103113	26.605930049490624
46	21.17992064556667	25.087985286953817	28.593585634305967	25.13850843317354
47	19.04104862224042	24.841679433505362	30.942007370643093	25.175264573611123
48	19.515171946372217	23.851116892469097	30.842064203666965	25.79164695749172
49	20.861117386669303	22.47618131499615	31.41352731868147	25.249173979653072
50	20.428383329920997	23.15336797441455	29.63409769632103	26.78415099934342
51	19.52421757517138	22.975808899839738	28.721415812994643	28.77855771199424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	889.0
2	1419.0
3	2490.5
4	3562.0
5	2745.5
6	1929.0
7	2031.5
8	2134.0
9	2296.5
10	2459.0
11	2669.5
12	2880.0
13	2950.0
14	3020.0
15	2999.0
16	2978.0
17	3194.5
18	3411.0
19	3809.5
20	4208.0
21	4909.0
22	5610.0
23	7901.5
24	10193.0
25	11918.5
26	17083.0
27	20522.0
28	24840.0
29	29158.0
30	33530.0
31	37902.0
32	43786.5
33	49671.0
34	58083.0
35	66495.0
36	71935.5
37	77376.0
38	86009.5
39	94643.0
40	105234.0
41	115825.0
42	127313.0
43	138801.0
44	152130.5
45	165460.0
46	192708.0
47	219956.0
48	239457.5
49	258959.0
50	248232.0
51	237505.0
52	206047.5
53	174590.0
54	153313.0
55	132036.0
56	118376.0
57	104716.0
58	92980.5
59	81245.0
60	73435.0
61	65625.0
62	57854.5
63	50084.0
64	42663.0
65	35242.0
66	27714.0
67	20186.0
68	16002.0
69	11818.0
70	10036.5
71	8255.0
72	6620.5
73	4986.0
74	4487.0
75	2886.5
76	1785.0
77	1422.0
78	1059.0
79	686.5
80	314.0
81	230.5
82	147.0
83	125.5
84	104.0
85	64.5
86	25.0
87	14.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2266288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.767658892437783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2296056221862	15.978903339012387
2	13.327745290196189	6.335386077253544
3	5.798915383322433	4.1347992833075295
4	2.9966423125297887	2.848926892274158
5	1.8135127275668688	2.155147595295189
6	1.112010126178559	1.5857926418369213
7	0.7744760747967362	1.288523821628607
8	0.5816072658100615	1.1058754482509547
9	0.440209623537274	0.9416476956062141
>10	3.976368255733128	22.457836951343037
>50	1.6012894071311994	27.100384109374907
>100	0.33753037785789275	10.964895372734265
>500	0.00726302387065507	1.1372156256292403
>1k	0.0020175066307375196	0.743870214217676
>5k	8.070026522950077E-4	1.2207949322354035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	6734	0.29713787479790743	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	6484	0.28610662016478045	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	6294	0.277722866643604	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	5947	0.2624114852128238	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	2796	0.12337355181689176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7650007413003114E-4	0.0	0.0	0.2953728740566071	0.0
2	1.7650007413003114E-4	0.0	0.0	1.183874247227184	0.0
3	1.7650007413003114E-4	0.0	0.0	1.7079470923377789	0.0
4	1.7650007413003114E-4	0.0	0.0	2.294677463764535	0.0
5	1.7650007413003114E-4	0.0	0.0	3.3035518874917926	0.0
6	2.2062509266253892E-4	0.0	0.0	4.080990589016047	0.0
7	2.2062509266253892E-4	0.0	0.0	4.7600746242313425	0.0
8	2.2062509266253892E-4	0.0	0.0	5.827017572347381	0.0
9	2.2062509266253892E-4	0.0	0.0	6.342044788658812	0.0
10	2.2062509266253892E-4	0.0	0.0	7.309529945002577	0.0
11	2.2062509266253892E-4	0.0	0.0	8.971234017918288	0.0
12	2.647501111950467E-4	0.0	0.0	10.327284087459317	0.0
13	2.647501111950467E-4	0.0	0.0	10.7717995241558	0.0
14	2.647501111950467E-4	0.0	0.0	10.937488968745367	0.0
15	2.647501111950467E-4	0.0	0.0	11.187942573935882	0.0
16	2.647501111950467E-4	0.0	0.0	11.854803979017671	0.0
17	2.647501111950467E-4	0.0	0.0	12.799255875687468	0.0
18	2.647501111950467E-4	0.0	0.0	13.86099207161667	0.0
19	2.647501111950467E-4	0.0	0.0	14.55035723615004	0.0
20	2.647501111950467E-4	0.0	0.0	15.226176019993929	0.0
21	3.088751297275545E-4	0.0	0.0	16.236771319443953	0.0
22	3.088751297275545E-4	0.0	0.0	17.2338202382045	0.0
23	3.088751297275545E-4	0.0	0.0	18.225265279611417	0.0
24	3.088751297275545E-4	0.0	0.0	18.996967728726446	0.0
25	3.088751297275545E-4	0.0	0.0	19.67481626342283	0.0
26	3.530001482600623E-4	0.0	0.0	20.266091511758436	0.0
27	3.9712516679257006E-4	0.0	0.0	20.85736676009404	0.0
28	3.9712516679257006E-4	0.0	0.0	21.487207274627057	0.0
29	3.9712516679257006E-4	0.0	0.0	22.168497560768976	0.0
30	4.4125018532507784E-4	0.0	0.0	22.861172101692283	0.0
31	4.4125018532507784E-4	0.0	0.0	23.534387509442755	0.0
32	4.4125018532507784E-4	0.0	0.0	24.150946393397486	0.0
33	4.4125018532507784E-4	0.0	0.0	24.793097788101072	0.0
34	4.4125018532507784E-4	0.0	0.0	25.40343504444272	0.0
35	4.4125018532507784E-4	0.0	0.0	26.061647945892137	0.0
36	4.4125018532507784E-4	0.0	0.0	26.686193458201252	0.0
37	4.4125018532507784E-4	0.0	0.0	27.320975974809908	0.0
38	4.4125018532507784E-4	0.0	0.0	27.957435242122802	0.0
39	4.4125018532507784E-4	0.0	0.0	28.601792887753014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAC	30	2.16644E-6	45.000004	11
TCTCGAT	30	2.16644E-6	45.000004	19
GCGATCG	30	2.16644E-6	45.000004	9
CCTTACG	40	6.8193913E-9	45.0	1
TCGTTAT	25	3.892011E-5	45.0	44
ATGGGCG	635	0.0	42.874016	5
TACGGGA	800	0.0	41.90625	4
CTTACGG	380	0.0	41.447372	2
TATCGCG	60	3.6379788E-12	41.250004	1
TTTACGG	1020	0.0	41.02941	2
CGAACGA	50	1.0822987E-9	40.5	22
TTACGGG	1665	0.0	40.27027	3
TTATGGG	5375	0.0	40.186047	3
CGTTTTT	1295	0.0	39.961388	1
TCACGGG	780	0.0	39.807693	3
TTTTACG	605	0.0	39.42149	1
AACGCAC	40	3.460209E-7	39.375	25
CCACGTC	40	3.460209E-7	39.375	33
TATTGCG	115	0.0	39.130432	1
ACGGGAT	450	0.0	39.0	5
>>END_MODULE