##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855824
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32863298996055	33.0	31.0	34.0	30.0	34.0
2	32.472702331320455	34.0	31.0	34.0	31.0	34.0
3	32.536571771766155	34.0	31.0	34.0	31.0	34.0
4	35.973139337059955	37.0	35.0	37.0	35.0	37.0
5	31.710257015461124	37.0	35.0	37.0	0.0	37.0
6	33.45168399110097	37.0	35.0	37.0	17.0	37.0
7	35.24404199928957	37.0	35.0	37.0	32.0	37.0
8	35.7901250724448	37.0	35.0	37.0	35.0	37.0
9	37.81200924489147	39.0	38.0	39.0	35.0	39.0
10	37.55146151545178	39.0	37.0	39.0	35.0	39.0
11	37.42800505711455	39.0	37.0	39.0	35.0	39.0
12	37.16289564209464	39.0	37.0	39.0	34.0	39.0
13	37.05505804931855	39.0	37.0	39.0	33.0	39.0
14	38.205250144889604	40.0	37.0	41.0	33.0	41.0
15	38.311380610966744	40.0	37.0	41.0	34.0	41.0
16	38.38300515059171	40.0	37.0	41.0	34.0	41.0
17	38.39313807511825	40.0	37.0	41.0	34.0	41.0
18	38.39744854082148	40.0	37.0	41.0	34.0	41.0
19	38.41029931387762	40.0	37.0	41.0	34.0	41.0
20	38.3321757744583	40.0	37.0	41.0	34.0	41.0
21	38.27350716969844	40.0	37.0	41.0	34.0	41.0
22	38.23208860700331	40.0	37.0	41.0	34.0	41.0
23	38.193140178354426	40.0	37.0	41.0	34.0	41.0
24	38.15419525509918	40.0	37.0	41.0	34.0	41.0
25	38.05968867430687	40.0	36.0	41.0	34.0	41.0
26	38.020479678064646	40.0	36.0	41.0	34.0	41.0
27	37.951997139598795	40.0	36.0	41.0	34.0	41.0
28	37.899494522238214	40.0	36.0	41.0	34.0	41.0
29	37.855263465385406	40.0	36.0	41.0	34.0	41.0
30	37.767542158200754	40.0	36.0	41.0	33.0	41.0
31	37.57075637046869	40.0	36.0	41.0	33.0	41.0
32	37.56553450242106	39.0	35.0	41.0	33.0	41.0
33	37.53976051150704	39.0	35.0	41.0	33.0	41.0
34	37.50421114621698	39.0	35.0	41.0	33.0	41.0
35	37.41706472358803	39.0	35.0	41.0	33.0	41.0
36	37.42423559049524	39.0	35.0	41.0	33.0	41.0
37	37.41844000635645	39.0	35.0	41.0	33.0	41.0
38	37.36403162332442	39.0	35.0	41.0	33.0	41.0
39	37.36022242891062	39.0	35.0	41.0	33.0	41.0
40	37.258082269251624	39.0	35.0	41.0	33.0	41.0
41	37.196563779468676	39.0	35.0	41.0	33.0	41.0
42	37.18783534932416	39.0	35.0	41.0	33.0	41.0
43	37.17693240666305	39.0	35.0	41.0	33.0	41.0
44	37.15139210865785	39.0	35.0	41.0	33.0	41.0
45	37.1450835685842	39.0	35.0	41.0	33.0	41.0
46	37.023892762998	39.0	35.0	41.0	32.0	41.0
47	36.96585863448559	39.0	35.0	41.0	32.0	41.0
48	36.91841196320739	39.0	35.0	40.0	32.0	41.0
49	36.91950915141431	39.0	35.0	40.0	32.0	41.0
50	36.84930546467498	39.0	35.0	40.0	32.0	41.0
51	36.19110821851222	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	11.0
12	10.0
13	7.0
14	8.0
15	20.0
16	25.0
17	40.0
18	83.0
19	153.0
20	252.0
21	442.0
22	711.0
23	1201.0
24	1831.0
25	2647.0
26	3575.0
27	4878.0
28	6107.0
29	8117.0
30	10615.0
31	14572.0
32	20444.0
33	31581.0
34	53960.0
35	82267.0
36	73474.0
37	113761.0
38	181570.0
39	243218.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.739722185869994	4.349375572547626	55.80247807960516	17.10842416197723
2	18.43895473835742	4.638336854306493	55.2222185869992	21.70048982033689
3	20.842719998504368	4.573370225653873	52.3948849296117	22.189024846230065
4	18.789143562227746	4.8493615509730965	51.451466656695764	24.910028230103386
5	15.817153994279199	17.113682252425733	44.88457907233263	22.18458468096244
6	21.478481556955636	6.349553179158332	52.37326833554563	19.798696928340405
7	83.08250294453066	2.3104049430724074	9.28496980687618	5.322122305520762
8	84.15316700630035	2.630564228159061	7.192366654826227	6.023902110714353
9	78.33655050571146	5.739030454859878	9.623357138850979	6.301061900577689
10	40.02832358054928	27.489063171867112	17.313255996560038	15.169357251023573
11	30.321187533885475	23.39873618874909	26.059329955691823	20.220746321673616
12	26.663776664360896	20.941572098936227	30.99504103647479	21.399610200228082
13	23.173105685281087	24.611952924900447	32.163505580586666	20.051435809231805
14	19.683486324290975	26.915697620071416	30.789741816074333	22.611074239563276
15	17.756454598141673	23.448395931873844	37.43550075716502	21.35964871281946
16	19.451546112284767	23.82662790480286	33.415047953784885	23.306778029127486
17	19.54327057899755	23.22930882985287	29.32588943521098	27.901531155938603
18	21.464927368243938	22.99748546430107	31.461258389575423	24.076328777879564
19	22.855400175737067	24.215376058628877	29.45535530669857	23.47386845893548
20	25.403821346445067	25.1524846230066	28.35746602105106	21.08622800949728
21	23.82896483389108	27.0586008338163	27.70908504552338	21.40334928676924
22	22.55942810671353	24.580988614481484	28.53577371048253	24.323809568322456
23	21.75050010282488	24.970671539942792	27.75430462338051	25.524523733851822
24	22.43218231785975	23.55846529192918	30.218362654003627	23.790989736207443
25	20.774715362037057	24.86702873488007	27.806067602684664	26.55218830039821
26	19.525860457290285	26.96185196956384	27.299187683448935	26.213099889696945
27	20.462501635850362	26.896184262184747	27.443259361737926	25.19805474022696
28	18.04144310045056	26.56083493802464	30.29489708164295	25.102824879881847
29	20.373347791134623	24.040807455738562	29.00584699657874	26.579997756548075
30	21.84292564826413	25.89539438015293	27.388925760436727	24.872754211146216
31	23.775098618407522	27.431808409205633	24.55598347323749	24.23710949914936
32	24.19481108265251	26.538984651049745	25.570911776253062	23.695292490044682
33	23.249406420011592	25.61823459028959	26.70642562022098	24.425933369477836
34	21.3527547720092	25.10118902952009	28.46087513320496	25.085181065265754
35	20.519289012694202	23.993952027519676	28.25651068443979	27.23024827534633
36	22.465366710912523	25.45383162893305	27.488362093140644	24.59243956701378
37	22.566906279795845	26.511408887808706	28.036839350146757	22.884845482248686
38	22.077670175176202	28.970676213800967	26.62942380676401	22.322229804258818
39	23.399203574566734	26.085737254388754	28.118047635962533	22.39701153508198
40	23.41252407036961	24.10939632447793	28.552599599917738	23.92548000523472
41	20.690936454224232	23.230360447942566	28.342509674886422	27.736193422946776
42	21.154700031782237	23.691436557049116	28.33888743479968	26.81497597636897
43	21.93079418198134	23.564541307558564	28.973831068070073	25.530833442390023
44	21.914318831909366	24.11909458019406	27.7730000560863	26.193586531810283
45	22.528230103385745	24.116991344014657	25.651769522705603	27.703009029893998
46	23.491862812914803	24.727747761221934	27.36263530819421	24.417754117669052
47	20.357456673334703	24.514035596103874	30.348763297126514	24.77974443343491
48	20.99882686159771	23.0071837200172	30.681658845743986	25.312330572641105
49	22.116229505131894	21.35684439791359	32.19914374918208	24.327782347772438
50	22.074865860270336	22.66575838022771	28.838522873861915	26.420852885640038
51	20.13159247695788	22.74451289050085	28.22578006692965	28.89811456561162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	451.0
2	642.0
3	1044.5
4	1447.0
5	1062.5
6	678.0
7	793.0
8	908.0
9	1040.5
10	1173.0
11	1186.0
12	1199.0
13	1210.5
14	1222.0
15	1249.5
16	1277.0
17	1246.0
18	1215.0
19	1276.0
20	1337.0
21	1732.5
22	2128.0
23	2990.0
24	3852.0
25	3985.5
26	6494.0
27	8869.0
28	8861.0
29	8853.0
30	10297.5
31	11742.0
32	13119.5
33	14497.0
34	17214.0
35	19931.0
36	21233.5
37	22536.0
38	25491.5
39	28447.0
40	33282.5
41	38118.0
42	45265.0
43	52412.0
44	58303.5
45	64195.0
46	75498.0
47	86801.0
48	94518.0
49	102235.0
50	97845.5
51	93456.0
52	78850.0
53	64244.0
54	57133.0
55	50022.0
56	45828.5
57	41635.0
58	37217.5
59	32800.0
60	30701.0
61	28602.0
62	24293.0
63	19984.0
64	18261.5
65	16539.0
66	13517.5
67	10496.0
68	8277.5
69	6059.0
70	5510.5
71	4962.0
72	3884.5
73	2807.0
74	2494.5
75	1549.5
76	917.0
77	781.0
78	645.0
79	442.0
80	239.0
81	184.0
82	129.0
83	70.0
84	11.0
85	6.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	855824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.301362195405865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.36848464683779	17.04514430605282
2	12.555552748154025	6.353451752891368
3	5.649137235221235	4.287926018396584
4	3.0221509517194383	3.0585814335457617
5	1.9514488450032685	2.4687157016617065
6	1.3515833935221204	2.051814058607936
7	0.993449316725741	1.7594934689679516
8	0.7271750606744118	1.4718815671671621
9	0.6266996870566555	1.4270720192971154
>10	4.081343910316167	22.095231109684267
>50	1.3938375821068845	25.079243255030175
>100	0.2689862000200342	9.784590228599646
>500	0.007843508405386933	1.293903434749081
>1k	0.0023069142368785096	1.8229516453483985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3909	0.4567527902933313	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	3482	0.4068593542597544	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3402	0.39751163790685934	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3353	0.3917861616407112	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	1470	0.1717642879844454	TruSeq Adapter, Index 15 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	894	0.1044607302436015	No Hit
GCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	879	0.10270803342743368	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1684645441118735E-4	0.0	0.0	0.4245031688758436	0.0
2	1.1684645441118735E-4	0.0	0.0	1.7717427882368337	0.0
3	1.1684645441118735E-4	0.0	0.0	2.507408065209669	0.0
4	1.1684645441118735E-4	0.0	0.0	3.3508057731496197	0.0
5	1.1684645441118735E-4	0.0	0.0	4.761960403073529	0.0
6	1.1684645441118735E-4	0.0	0.0	5.726527884237881	0.0
7	1.1684645441118735E-4	0.0	0.0	6.695535530669857	0.0
8	1.1684645441118735E-4	0.0	0.0	8.208580268840322	0.0
9	1.1684645441118735E-4	0.0	0.0	8.974275084596833	0.0
10	1.1684645441118735E-4	0.0	0.0	10.248836209314064	0.0
11	1.1684645441118735E-4	0.0	0.0	12.319589074389127	0.0
12	1.1684645441118735E-4	0.0	0.0	14.197428443231319	0.0
13	1.1684645441118735E-4	0.0	0.0	14.800356147993046	0.0
14	2.336929088223747E-4	0.0	0.0	15.019793789377255	0.0
15	2.336929088223747E-4	0.0	0.0	15.3597001252594	0.0
16	2.336929088223747E-4	0.0	0.0	16.178092691955356	0.0
17	2.336929088223747E-4	0.0	0.0	17.418184112621287	0.0
18	2.336929088223747E-4	0.0	0.0	18.862873674961207	0.0
19	2.336929088223747E-4	0.0	0.0	19.70837461908056	0.0
20	2.336929088223747E-4	0.0	0.0	20.61486941240255	0.0
21	2.336929088223747E-4	0.0	0.0	21.73963338256464	0.0
22	2.336929088223747E-4	0.0	0.0	22.817541924507843	0.0
23	2.336929088223747E-4	0.0	0.0	23.822538278898467	0.0
24	2.336929088223747E-4	0.0	0.0	24.642449849501766	0.0
25	2.336929088223747E-4	0.0	0.0	25.35310998523061	0.0
26	2.336929088223747E-4	0.0	0.0	25.99681710258184	0.0
27	2.336929088223747E-4	0.0	0.0	26.600562732524445	0.0
28	2.336929088223747E-4	0.0	0.0	27.258875656677073	0.0
29	2.336929088223747E-4	0.0	0.0	27.940324178803117	0.0
30	2.336929088223747E-4	0.0	0.0	28.71478247864047	0.0
31	2.336929088223747E-4	0.0	0.0	29.419249752285516	0.0
32	2.336929088223747E-4	0.0	0.0	30.067280188449963	0.0
33	2.336929088223747E-4	0.0	0.0	30.671610050664622	0.0
34	2.336929088223747E-4	0.0	0.0	31.28271700723513	0.0
35	2.336929088223747E-4	0.0	0.0	31.947105947017143	0.0
36	2.336929088223747E-4	0.0	0.0	32.53519415206865	0.0
37	2.336929088223747E-4	0.0	0.0	33.16709977752435	0.0
38	2.336929088223747E-4	0.0	0.0	33.798421170708	0.0
39	2.336929088223747E-4	0.0	0.0	34.431845800071045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.211447E-7	45.000004	36
AAACGTA	35	1.211447E-7	45.000004	35
TTTTACG	175	0.0	45.000004	1
TGATCGA	20	7.032277E-4	45.0	32
TCACGCG	20	7.032277E-4	45.0	23
TATCACT	20	7.032277E-4	45.0	29
GGTACGC	25	3.8899674E-5	45.0	8
ACACGAT	20	7.032277E-4	45.0	39
GCGACGT	20	7.032277E-4	45.0	15
CTCCGTA	20	7.032277E-4	45.0	21
TAGACTC	20	7.032277E-4	45.0	19
TCCGATT	20	7.032277E-4	45.0	25
CTTCACG	25	3.8899674E-5	45.0	1
CGTTGAC	20	7.032277E-4	45.0	21
TAGCGAC	20	7.032277E-4	45.0	12
ATAGCGA	20	7.032277E-4	45.0	11
CACGCGA	20	7.032277E-4	45.0	13
CACGATT	25	3.8899674E-5	45.0	13
ACGGGTA	25	3.8899674E-5	45.0	5
TGCATCG	20	7.032277E-4	45.0	18
>>END_MODULE