FastQCFastQC Report
Sat 14 Jan 2017
SRR2935345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008403
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC61280.20369611385176786No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC59260.1969815879056097No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT57180.1900676205947142TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC53000.17617320551801072No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG37910.126013702286562No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32860.10922738742116665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG21200.041.6037753
TACGGGA13000.040.54
TTATGGG65650.040.373193
CGATAAC1900.040.26315716
TTCACGG6500.040.1538472
ATAACGC451.9301297E-840.011
TCACGGG11200.039.977683
TTTACGG14000.039.8571432
TATGGGA32800.039.8551834
TATGGGT10700.039.742994
ATGGGCG9000.039.55
AAGCGTA403.4607183E-739.37500419
TACGGGG18650.039.3297584
CTACGGG10700.039.322433
GTACGGG6650.039.2481233
TTTCACG4500.039.01
TACGGGT2550.038.823534
CCTACGG1450.038.7931022
TATGGGC19450.038.637534
AACGCGT356.251659E-638.5714314
Produced by FastQC (version 0.11.5)