##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3008403
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38890434559465	33.0	31.0	34.0	31.0	34.0
2	32.53203443820525	34.0	31.0	34.0	31.0	34.0
3	32.590482392152914	34.0	31.0	34.0	31.0	34.0
4	36.01221212716514	37.0	35.0	37.0	35.0	37.0
5	31.670366303982544	37.0	35.0	37.0	0.0	37.0
6	33.415124901816675	37.0	35.0	37.0	17.0	37.0
7	35.219673693983154	37.0	35.0	37.0	32.0	37.0
8	35.78153990672127	37.0	35.0	37.0	35.0	37.0
9	37.823807515150065	39.0	38.0	39.0	35.0	39.0
10	37.58841850643016	39.0	37.0	39.0	35.0	39.0
11	37.47981969171018	39.0	37.0	39.0	35.0	39.0
12	37.0938650839	39.0	37.0	39.0	34.0	39.0
13	36.94706726459188	39.0	35.0	39.0	33.0	39.0
14	37.77667353742168	40.0	37.0	41.0	33.0	41.0
15	37.96779819724951	40.0	37.0	41.0	33.0	41.0
16	38.161564125550996	40.0	37.0	41.0	33.0	41.0
17	38.22389686488147	40.0	37.0	41.0	34.0	41.0
18	38.21153715110642	40.0	37.0	41.0	34.0	41.0
19	38.23368312024686	40.0	37.0	41.0	34.0	41.0
20	38.12636538389305	40.0	36.0	41.0	33.0	41.0
21	38.08933610290909	40.0	36.0	41.0	34.0	41.0
22	38.13432708317337	40.0	36.0	41.0	34.0	41.0
23	38.1114953681405	40.0	36.0	41.0	34.0	41.0
24	38.07410243906817	40.0	36.0	41.0	34.0	41.0
25	37.98782609909643	40.0	36.0	41.0	34.0	41.0
26	37.93507984136434	40.0	36.0	41.0	34.0	41.0
27	37.87947326205964	40.0	35.0	41.0	34.0	41.0
28	37.82754404911842	40.0	35.0	41.0	34.0	41.0
29	37.72647314871046	40.0	35.0	41.0	33.0	41.0
30	37.65907459871566	40.0	35.0	41.0	33.0	41.0
31	37.4173865668928	40.0	35.0	41.0	33.0	41.0
32	37.47284921601261	40.0	35.0	41.0	33.0	41.0
33	37.4602903932751	39.0	35.0	41.0	33.0	41.0
34	37.35009372082131	39.0	35.0	41.0	32.0	41.0
35	37.25567485473189	39.0	35.0	41.0	32.0	41.0
36	37.248230040988524	39.0	35.0	41.0	32.0	41.0
37	37.23209191055852	39.0	35.0	41.0	32.0	41.0
38	37.20212052707034	39.0	35.0	41.0	32.0	41.0
39	37.169791746650965	39.0	35.0	41.0	32.0	41.0
40	37.08040279178022	39.0	35.0	41.0	32.0	41.0
41	36.97245648272522	39.0	35.0	41.0	32.0	41.0
42	36.98899050426422	39.0	35.0	41.0	32.0	41.0
43	36.94132468289654	39.0	35.0	41.0	32.0	41.0
44	36.90542424003699	39.0	35.0	41.0	32.0	41.0
45	36.88389387990904	39.0	35.0	41.0	32.0	41.0
46	36.81556493594774	39.0	35.0	41.0	32.0	41.0
47	36.747703681986756	38.0	35.0	41.0	32.0	41.0
48	36.6780713887069	38.0	35.0	41.0	32.0	41.0
49	36.63965964666303	38.0	35.0	41.0	32.0	41.0
50	36.55767761167636	38.0	35.0	40.0	32.0	41.0
51	35.891087729935116	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	30.0
11	31.0
12	35.0
13	53.0
14	62.0
15	97.0
16	183.0
17	260.0
18	523.0
19	919.0
20	1449.0
21	2501.0
22	3738.0
23	5557.0
24	8000.0
25	11688.0
26	15799.0
27	20499.0
28	25573.0
29	32176.0
30	42063.0
31	57029.0
32	79898.0
33	129228.0
34	228379.0
35	238538.0
36	243055.0
37	379769.0
38	597248.0
39	883017.0
40	991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.512739815776012	4.687337434512597	54.64474008302744	16.155182666683952
2	19.958064129041222	4.560592447221998	52.96836893195492	22.512974491781854
3	21.686190314263083	4.820863428204267	50.990309476489685	22.502636781042966
4	19.837668025194763	5.032503956418073	49.099804780144154	26.030023238243018
5	16.591261210682212	17.853425887422663	42.745071055972225	22.810241845922903
6	21.882540337847022	6.993511175198269	51.860704832431026	19.26324365452368
7	83.68207317969035	2.7953701681589864	8.510129793116148	5.012426859034512
8	85.72382091096173	3.1681925593080447	6.388239873447805	4.719746656282419
9	80.58438314281698	5.151105087981897	8.78146976984134	5.483041999359793
10	43.25195128445225	24.387756560540595	16.51374500025429	15.846547154752871
11	34.142433709845385	23.7700534137215	22.944166722344047	19.143346154089063
12	30.243155587865054	21.125095274801946	28.294979096882965	20.336770040450034
13	28.100557006491485	20.988677381321587	30.297237437936342	20.613528174250593
14	22.42804571063119	22.511910804503252	33.84902222208926	21.2110212627763
15	20.29993986842853	22.542259132170788	35.67048696600821	21.48731403339247
16	26.356907635047566	22.330419162592246	29.726037369328512	21.58663583303168
17	27.321273114007667	22.736215859377882	27.11884012879923	22.82367089781522
18	27.59144968277189	21.795185020092056	29.34563620631943	21.267729090816623
19	27.649453879683005	24.115818259721188	26.809772493911222	21.424955366684586
20	25.559474578372644	28.243656185690547	27.11136107762158	19.085508158315225
21	25.207759731658292	25.39240919517764	30.251698326321307	19.148132746842762
22	24.132671055041495	22.300569438336552	31.41706746070922	22.14969204591273
23	22.901419789835337	23.3815416352131	30.697516256964242	23.019522317987317
24	23.775205648977217	25.786405611216317	28.06951063404737	22.3688781057591
25	25.546976252849102	24.57825630409224	25.78866594668334	24.08610149637532
26	21.959923587365125	24.25160458887988	26.62974342200829	27.158728401746707
27	21.80738418356849	23.4984142749492	30.07801813786251	24.616183403619793
28	20.302000762530817	24.473316906012922	28.155901985206107	27.068780346250154
29	22.437652136366037	26.333905397647854	26.567019112798384	24.661423353187722
30	25.965869599252496	24.536240656587566	26.80239316341594	22.695496580744003
31	24.514102665101717	24.674686203942755	26.978001285067194	23.833209845888334
32	26.229597563890213	24.761675879195703	25.12279771028017	23.88592884663391
33	26.74246103331236	24.99203065546737	25.052760551029902	23.212747760190375
34	22.057417174494244	24.69316777040842	28.762070773097886	24.487344281999455
35	21.659731093207924	27.420428712509594	26.689110468245115	24.23072972603737
36	24.69679095520115	26.867544009230148	24.974911938327413	23.460753097241295
37	22.989240470774693	28.294979096882965	25.262439905823786	23.453340526518556
38	23.448221531490297	27.73278712991577	25.303092704002754	23.515898634591174
39	22.479202420686324	26.117910399637285	25.241731244118558	26.161155935557833
40	23.78617492403777	23.895967395325695	27.616811976320992	24.701045704315547
41	21.209292770948572	23.64433887348204	27.561899120563304	27.584469235006082
42	21.141648908075148	23.98757746219506	29.04334957783249	25.827424051897303
43	21.099998903072496	24.04810791639285	29.31788061639348	25.53401256414117
44	22.74512424033615	23.394139681419013	27.576990183828432	26.283745894416406
45	21.828259046411002	22.893043252516367	26.30299198611356	28.975705714959066
46	22.18432836292212	23.487976843527942	27.70719215477448	26.620502638775456
47	19.85515238483674	25.283913092760514	29.981887400059097	24.879047122343646
48	20.10977252715145	26.863854343982506	27.91550866024266	25.110864468623383
49	22.776270333462637	23.981527740798025	28.45921906074419	24.782982864995148
50	20.880713122543753	24.249876097052155	27.437314748057357	27.43209603234673
51	20.5639337548859	24.74236330704364	26.13330062494952	28.560402313120946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	517.0
1	1595.5
2	2674.0
3	4932.5
4	7191.0
5	5099.0
6	3007.0
7	3062.5
8	3118.0
9	3364.5
10	3611.0
11	3698.5
12	3786.0
13	3836.0
14	3886.0
15	3803.5
16	3721.0
17	3808.0
18	3895.0
19	4401.5
20	4908.0
21	6066.0
22	7224.0
23	9019.5
24	10815.0
25	13365.5
26	19546.5
27	23177.0
28	29240.5
29	35304.0
30	39697.0
31	44090.0
32	50248.5
33	56407.0
34	63920.0
35	71433.0
36	79043.0
37	86653.0
38	93989.0
39	101325.0
40	112121.5
41	122918.0
42	136092.0
43	149266.0
44	163243.5
45	177221.0
46	204286.5
47	231352.0
48	263275.5
49	295199.0
50	290561.0
51	285923.0
52	258103.5
53	230284.0
54	218661.0
55	207038.0
56	196948.5
57	186859.0
58	173514.5
59	160170.0
60	144916.0
61	129662.0
62	114572.0
63	99482.0
64	89653.5
65	79825.0
66	67595.5
67	55366.0
68	47089.0
69	38812.0
70	32168.5
71	25525.0
72	21839.0
73	18153.0
74	14906.5
75	8645.5
76	5631.0
77	4464.5
78	3298.0
79	2305.0
80	1312.0
81	901.5
82	491.0
83	343.0
84	195.0
85	123.5
86	52.0
87	40.5
88	29.0
89	15.5
90	2.0
91	9.0
92	16.0
93	9.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3008403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.306447210077764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.93785320354962	15.377585832610698
2	12.41762988300013	5.53986410918853
3	5.155318522580065	3.4499052142520497
4	2.592993340435482	2.3136187625802913
5	1.6302971897851213	1.8183069100339968
6	1.0222370365414524	1.3681485895078944
7	0.7092224627121932	1.1074163397283618
8	0.5318580108423844	0.949109011369009
9	0.4311466949206997	0.8655615891043281
>10	4.321805267016014	23.335334348694257
>50	1.8937662302999072	30.06009187655185
>100	0.34552081561926284	10.871313552237694
>500	0.007440027563964486	1.1480503658503767
>1k	0.0022643562151196265	0.9632929439114125
>5k	6.469589186056076E-4	0.83240055437918
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	6128	0.20369611385176786	No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	5926	0.1969815879056097	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	5718	0.1900676205947142	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	5300	0.17617320551801072	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3791	0.126013702286562	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3286	0.10922738742116665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.97206823686853E-5	0.0	0.0	0.19794555450184034	0.0
2	9.97206823686853E-5	0.0	0.0	0.8127568015322415	0.0
3	9.97206823686853E-5	0.0	0.0	1.193490366815882	0.0
4	9.97206823686853E-5	0.0	0.0	1.6333915369716092	0.0
5	9.97206823686853E-5	0.0	0.0	2.4281986156774873	0.0
6	9.97206823686853E-5	0.0	0.0	3.1331241193417236	0.0
7	9.97206823686853E-5	0.0	0.0	3.749663858199849	0.0
8	9.97206823686853E-5	0.0	0.0	4.711769001692924	0.0
9	9.97206823686853E-5	0.0	0.0	5.228056214543065	0.0
10	1.3296090982491376E-4	0.0	0.0	6.100279782994499	0.0
11	1.3296090982491376E-4	0.0	0.0	7.561553422197758	0.0
12	1.3296090982491376E-4	0.0	0.0	8.75670580038645	0.0
13	1.3296090982491376E-4	0.0	0.0	9.154092719625663	0.0
14	1.662011372811422E-4	0.0	0.0	9.325379611707607	0.0
15	1.662011372811422E-4	0.0	0.0	9.557063997077519	0.0
16	1.662011372811422E-4	0.0	0.0	10.05649841460735	0.0
17	1.662011372811422E-4	0.0	0.0	10.749158274340239	0.0
18	1.994413647373706E-4	0.0	0.0	11.500719817125564	0.0
19	1.994413647373706E-4	0.0	0.0	12.042302843069894	0.0
20	1.994413647373706E-4	0.0	0.0	12.640793138419287	0.0
21	2.659218196498275E-4	0.0	0.0	13.436929826223414	0.0
22	2.659218196498275E-4	0.0	0.0	14.268832998770444	0.0
23	2.659218196498275E-4	0.0	0.0	15.002378338274493	0.0
24	2.659218196498275E-4	0.0	0.0	15.5806585753305	0.0
25	2.9916204710605593E-4	0.0	0.0	16.105255845044695	0.0
26	2.9916204710605593E-4	0.0	0.0	16.600169591640483	0.0
27	2.9916204710605593E-4	0.0	0.0	17.18908670148248	0.0
28	2.9916204710605593E-4	0.0	0.0	17.720032854640817	0.0
29	2.9916204710605593E-4	0.0	0.0	18.349868684481436	0.0
30	2.9916204710605593E-4	0.0	0.0	18.98226401183618	0.0
31	3.324022745622844E-4	0.0	0.0	19.515271059096804	0.0
32	3.324022745622844E-4	0.0	0.0	20.06360185121475	0.0
33	3.324022745622844E-4	0.0	0.0	20.604619793292322	0.0
34	3.324022745622844E-4	0.0	0.0	21.194367908820727	0.0
35	3.324022745622844E-4	0.0	0.0	21.795650383276442	0.0
36	3.324022745622844E-4	0.0	0.0	22.327294581211362	0.0
37	3.324022745622844E-4	0.0	0.0	22.891381241143556	0.0
38	3.324022745622844E-4	0.0	0.0	23.64357434825055	0.0
39	3.656425020185128E-4	0.0	0.0	25.091319214879125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGG	2120	0.0	41.603775	3
TACGGGA	1300	0.0	40.5	4
TTATGGG	6565	0.0	40.37319	3
CGATAAC	190	0.0	40.263157	16
TTCACGG	650	0.0	40.153847	2
ATAACGC	45	1.9301297E-8	40.0	11
TCACGGG	1120	0.0	39.97768	3
TTTACGG	1400	0.0	39.857143	2
TATGGGA	3280	0.0	39.855183	4
TATGGGT	1070	0.0	39.74299	4
ATGGGCG	900	0.0	39.5	5
AAGCGTA	40	3.4607183E-7	39.375004	19
TACGGGG	1865	0.0	39.329758	4
CTACGGG	1070	0.0	39.32243	3
GTACGGG	665	0.0	39.248123	3
TTTCACG	450	0.0	39.0	1
TACGGGT	255	0.0	38.82353	4
CCTACGG	145	0.0	38.793102	2
TATGGGC	1945	0.0	38.63753	4
AACGCGT	35	6.251659E-6	38.57143	14
>>END_MODULE