##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935345.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3008403 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38890434559465 33.0 31.0 34.0 31.0 34.0 2 32.53203443820525 34.0 31.0 34.0 31.0 34.0 3 32.590482392152914 34.0 31.0 34.0 31.0 34.0 4 36.01221212716514 37.0 35.0 37.0 35.0 37.0 5 31.670366303982544 37.0 35.0 37.0 0.0 37.0 6 33.415124901816675 37.0 35.0 37.0 17.0 37.0 7 35.219673693983154 37.0 35.0 37.0 32.0 37.0 8 35.78153990672127 37.0 35.0 37.0 35.0 37.0 9 37.823807515150065 39.0 38.0 39.0 35.0 39.0 10 37.58841850643016 39.0 37.0 39.0 35.0 39.0 11 37.47981969171018 39.0 37.0 39.0 35.0 39.0 12 37.0938650839 39.0 37.0 39.0 34.0 39.0 13 36.94706726459188 39.0 35.0 39.0 33.0 39.0 14 37.77667353742168 40.0 37.0 41.0 33.0 41.0 15 37.96779819724951 40.0 37.0 41.0 33.0 41.0 16 38.161564125550996 40.0 37.0 41.0 33.0 41.0 17 38.22389686488147 40.0 37.0 41.0 34.0 41.0 18 38.21153715110642 40.0 37.0 41.0 34.0 41.0 19 38.23368312024686 40.0 37.0 41.0 34.0 41.0 20 38.12636538389305 40.0 36.0 41.0 33.0 41.0 21 38.08933610290909 40.0 36.0 41.0 34.0 41.0 22 38.13432708317337 40.0 36.0 41.0 34.0 41.0 23 38.1114953681405 40.0 36.0 41.0 34.0 41.0 24 38.07410243906817 40.0 36.0 41.0 34.0 41.0 25 37.98782609909643 40.0 36.0 41.0 34.0 41.0 26 37.93507984136434 40.0 36.0 41.0 34.0 41.0 27 37.87947326205964 40.0 35.0 41.0 34.0 41.0 28 37.82754404911842 40.0 35.0 41.0 34.0 41.0 29 37.72647314871046 40.0 35.0 41.0 33.0 41.0 30 37.65907459871566 40.0 35.0 41.0 33.0 41.0 31 37.4173865668928 40.0 35.0 41.0 33.0 41.0 32 37.47284921601261 40.0 35.0 41.0 33.0 41.0 33 37.4602903932751 39.0 35.0 41.0 33.0 41.0 34 37.35009372082131 39.0 35.0 41.0 32.0 41.0 35 37.25567485473189 39.0 35.0 41.0 32.0 41.0 36 37.248230040988524 39.0 35.0 41.0 32.0 41.0 37 37.23209191055852 39.0 35.0 41.0 32.0 41.0 38 37.20212052707034 39.0 35.0 41.0 32.0 41.0 39 37.169791746650965 39.0 35.0 41.0 32.0 41.0 40 37.08040279178022 39.0 35.0 41.0 32.0 41.0 41 36.97245648272522 39.0 35.0 41.0 32.0 41.0 42 36.98899050426422 39.0 35.0 41.0 32.0 41.0 43 36.94132468289654 39.0 35.0 41.0 32.0 41.0 44 36.90542424003699 39.0 35.0 41.0 32.0 41.0 45 36.88389387990904 39.0 35.0 41.0 32.0 41.0 46 36.81556493594774 39.0 35.0 41.0 32.0 41.0 47 36.747703681986756 38.0 35.0 41.0 32.0 41.0 48 36.6780713887069 38.0 35.0 41.0 32.0 41.0 49 36.63965964666303 38.0 35.0 41.0 32.0 41.0 50 36.55767761167636 38.0 35.0 40.0 32.0 41.0 51 35.891087729935116 37.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 11.0 10 30.0 11 31.0 12 35.0 13 53.0 14 62.0 15 97.0 16 183.0 17 260.0 18 523.0 19 919.0 20 1449.0 21 2501.0 22 3738.0 23 5557.0 24 8000.0 25 11688.0 26 15799.0 27 20499.0 28 25573.0 29 32176.0 30 42063.0 31 57029.0 32 79898.0 33 129228.0 34 228379.0 35 238538.0 36 243055.0 37 379769.0 38 597248.0 39 883017.0 40 991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.512739815776012 4.687337434512597 54.64474008302744 16.155182666683952 2 19.958064129041222 4.560592447221998 52.96836893195492 22.512974491781854 3 21.686190314263083 4.820863428204267 50.990309476489685 22.502636781042966 4 19.837668025194763 5.032503956418073 49.099804780144154 26.030023238243018 5 16.591261210682212 17.853425887422663 42.745071055972225 22.810241845922903 6 21.882540337847022 6.993511175198269 51.860704832431026 19.26324365452368 7 83.68207317969035 2.7953701681589864 8.510129793116148 5.012426859034512 8 85.72382091096173 3.1681925593080447 6.388239873447805 4.719746656282419 9 80.58438314281698 5.151105087981897 8.78146976984134 5.483041999359793 10 43.25195128445225 24.387756560540595 16.51374500025429 15.846547154752871 11 34.142433709845385 23.7700534137215 22.944166722344047 19.143346154089063 12 30.243155587865054 21.125095274801946 28.294979096882965 20.336770040450034 13 28.100557006491485 20.988677381321587 30.297237437936342 20.613528174250593 14 22.42804571063119 22.511910804503252 33.84902222208926 21.2110212627763 15 20.29993986842853 22.542259132170788 35.67048696600821 21.48731403339247 16 26.356907635047566 22.330419162592246 29.726037369328512 21.58663583303168 17 27.321273114007667 22.736215859377882 27.11884012879923 22.82367089781522 18 27.59144968277189 21.795185020092056 29.34563620631943 21.267729090816623 19 27.649453879683005 24.115818259721188 26.809772493911222 21.424955366684586 20 25.559474578372644 28.243656185690547 27.11136107762158 19.085508158315225 21 25.207759731658292 25.39240919517764 30.251698326321307 19.148132746842762 22 24.132671055041495 22.300569438336552 31.41706746070922 22.14969204591273 23 22.901419789835337 23.3815416352131 30.697516256964242 23.019522317987317 24 23.775205648977217 25.786405611216317 28.06951063404737 22.3688781057591 25 25.546976252849102 24.57825630409224 25.78866594668334 24.08610149637532 26 21.959923587365125 24.25160458887988 26.62974342200829 27.158728401746707 27 21.80738418356849 23.4984142749492 30.07801813786251 24.616183403619793 28 20.302000762530817 24.473316906012922 28.155901985206107 27.068780346250154 29 22.437652136366037 26.333905397647854 26.567019112798384 24.661423353187722 30 25.965869599252496 24.536240656587566 26.80239316341594 22.695496580744003 31 24.514102665101717 24.674686203942755 26.978001285067194 23.833209845888334 32 26.229597563890213 24.761675879195703 25.12279771028017 23.88592884663391 33 26.74246103331236 24.99203065546737 25.052760551029902 23.212747760190375 34 22.057417174494244 24.69316777040842 28.762070773097886 24.487344281999455 35 21.659731093207924 27.420428712509594 26.689110468245115 24.23072972603737 36 24.69679095520115 26.867544009230148 24.974911938327413 23.460753097241295 37 22.989240470774693 28.294979096882965 25.262439905823786 23.453340526518556 38 23.448221531490297 27.73278712991577 25.303092704002754 23.515898634591174 39 22.479202420686324 26.117910399637285 25.241731244118558 26.161155935557833 40 23.78617492403777 23.895967395325695 27.616811976320992 24.701045704315547 41 21.209292770948572 23.64433887348204 27.561899120563304 27.584469235006082 42 21.141648908075148 23.98757746219506 29.04334957783249 25.827424051897303 43 21.099998903072496 24.04810791639285 29.31788061639348 25.53401256414117 44 22.74512424033615 23.394139681419013 27.576990183828432 26.283745894416406 45 21.828259046411002 22.893043252516367 26.30299198611356 28.975705714959066 46 22.18432836292212 23.487976843527942 27.70719215477448 26.620502638775456 47 19.85515238483674 25.283913092760514 29.981887400059097 24.879047122343646 48 20.10977252715145 26.863854343982506 27.91550866024266 25.110864468623383 49 22.776270333462637 23.981527740798025 28.45921906074419 24.782982864995148 50 20.880713122543753 24.249876097052155 27.437314748057357 27.43209603234673 51 20.5639337548859 24.74236330704364 26.13330062494952 28.560402313120946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 517.0 1 1595.5 2 2674.0 3 4932.5 4 7191.0 5 5099.0 6 3007.0 7 3062.5 8 3118.0 9 3364.5 10 3611.0 11 3698.5 12 3786.0 13 3836.0 14 3886.0 15 3803.5 16 3721.0 17 3808.0 18 3895.0 19 4401.5 20 4908.0 21 6066.0 22 7224.0 23 9019.5 24 10815.0 25 13365.5 26 19546.5 27 23177.0 28 29240.5 29 35304.0 30 39697.0 31 44090.0 32 50248.5 33 56407.0 34 63920.0 35 71433.0 36 79043.0 37 86653.0 38 93989.0 39 101325.0 40 112121.5 41 122918.0 42 136092.0 43 149266.0 44 163243.5 45 177221.0 46 204286.5 47 231352.0 48 263275.5 49 295199.0 50 290561.0 51 285923.0 52 258103.5 53 230284.0 54 218661.0 55 207038.0 56 196948.5 57 186859.0 58 173514.5 59 160170.0 60 144916.0 61 129662.0 62 114572.0 63 99482.0 64 89653.5 65 79825.0 66 67595.5 67 55366.0 68 47089.0 69 38812.0 70 32168.5 71 25525.0 72 21839.0 73 18153.0 74 14906.5 75 8645.5 76 5631.0 77 4464.5 78 3298.0 79 2305.0 80 1312.0 81 901.5 82 491.0 83 343.0 84 195.0 85 123.5 86 52.0 87 40.5 88 29.0 89 15.5 90 2.0 91 9.0 92 16.0 93 9.5 94 3.0 95 1.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3008403.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.306447210077764 #Duplication Level Percentage of deduplicated Percentage of total 1 68.93785320354962 15.377585832610698 2 12.41762988300013 5.53986410918853 3 5.155318522580065 3.4499052142520497 4 2.592993340435482 2.3136187625802913 5 1.6302971897851213 1.8183069100339968 6 1.0222370365414524 1.3681485895078944 7 0.7092224627121932 1.1074163397283618 8 0.5318580108423844 0.949109011369009 9 0.4311466949206997 0.8655615891043281 >10 4.321805267016014 23.335334348694257 >50 1.8937662302999072 30.06009187655185 >100 0.34552081561926284 10.871313552237694 >500 0.007440027563964486 1.1480503658503767 >1k 0.0022643562151196265 0.9632929439114125 >5k 6.469589186056076E-4 0.83240055437918 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC 6128 0.20369611385176786 No Hit GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC 5926 0.1969815879056097 No Hit CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT 5718 0.1900676205947142 TruSeq Adapter, Index 13 (96% over 25bp) CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC 5300 0.17617320551801072 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 3791 0.126013702286562 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3286 0.10922738742116665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.97206823686853E-5 0.0 0.0 0.19794555450184034 0.0 2 9.97206823686853E-5 0.0 0.0 0.8127568015322415 0.0 3 9.97206823686853E-5 0.0 0.0 1.193490366815882 0.0 4 9.97206823686853E-5 0.0 0.0 1.6333915369716092 0.0 5 9.97206823686853E-5 0.0 0.0 2.4281986156774873 0.0 6 9.97206823686853E-5 0.0 0.0 3.1331241193417236 0.0 7 9.97206823686853E-5 0.0 0.0 3.749663858199849 0.0 8 9.97206823686853E-5 0.0 0.0 4.711769001692924 0.0 9 9.97206823686853E-5 0.0 0.0 5.228056214543065 0.0 10 1.3296090982491376E-4 0.0 0.0 6.100279782994499 0.0 11 1.3296090982491376E-4 0.0 0.0 7.561553422197758 0.0 12 1.3296090982491376E-4 0.0 0.0 8.75670580038645 0.0 13 1.3296090982491376E-4 0.0 0.0 9.154092719625663 0.0 14 1.662011372811422E-4 0.0 0.0 9.325379611707607 0.0 15 1.662011372811422E-4 0.0 0.0 9.557063997077519 0.0 16 1.662011372811422E-4 0.0 0.0 10.05649841460735 0.0 17 1.662011372811422E-4 0.0 0.0 10.749158274340239 0.0 18 1.994413647373706E-4 0.0 0.0 11.500719817125564 0.0 19 1.994413647373706E-4 0.0 0.0 12.042302843069894 0.0 20 1.994413647373706E-4 0.0 0.0 12.640793138419287 0.0 21 2.659218196498275E-4 0.0 0.0 13.436929826223414 0.0 22 2.659218196498275E-4 0.0 0.0 14.268832998770444 0.0 23 2.659218196498275E-4 0.0 0.0 15.002378338274493 0.0 24 2.659218196498275E-4 0.0 0.0 15.5806585753305 0.0 25 2.9916204710605593E-4 0.0 0.0 16.105255845044695 0.0 26 2.9916204710605593E-4 0.0 0.0 16.600169591640483 0.0 27 2.9916204710605593E-4 0.0 0.0 17.18908670148248 0.0 28 2.9916204710605593E-4 0.0 0.0 17.720032854640817 0.0 29 2.9916204710605593E-4 0.0 0.0 18.349868684481436 0.0 30 2.9916204710605593E-4 0.0 0.0 18.98226401183618 0.0 31 3.324022745622844E-4 0.0 0.0 19.515271059096804 0.0 32 3.324022745622844E-4 0.0 0.0 20.06360185121475 0.0 33 3.324022745622844E-4 0.0 0.0 20.604619793292322 0.0 34 3.324022745622844E-4 0.0 0.0 21.194367908820727 0.0 35 3.324022745622844E-4 0.0 0.0 21.795650383276442 0.0 36 3.324022745622844E-4 0.0 0.0 22.327294581211362 0.0 37 3.324022745622844E-4 0.0 0.0 22.891381241143556 0.0 38 3.324022745622844E-4 0.0 0.0 23.64357434825055 0.0 39 3.656425020185128E-4 0.0 0.0 25.091319214879125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGGG 2120 0.0 41.603775 3 TACGGGA 1300 0.0 40.5 4 TTATGGG 6565 0.0 40.37319 3 CGATAAC 190 0.0 40.263157 16 TTCACGG 650 0.0 40.153847 2 ATAACGC 45 1.9301297E-8 40.0 11 TCACGGG 1120 0.0 39.97768 3 TTTACGG 1400 0.0 39.857143 2 TATGGGA 3280 0.0 39.855183 4 TATGGGT 1070 0.0 39.74299 4 ATGGGCG 900 0.0 39.5 5 AAGCGTA 40 3.4607183E-7 39.375004 19 TACGGGG 1865 0.0 39.329758 4 CTACGGG 1070 0.0 39.32243 3 GTACGGG 665 0.0 39.248123 3 TTTCACG 450 0.0 39.0 1 TACGGGT 255 0.0 38.82353 4 CCTACGG 145 0.0 38.793102 2 TATGGGC 1945 0.0 38.63753 4 AACGCGT 35 6.251659E-6 38.57143 14 >>END_MODULE