##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890500
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41109152161707	33.0	31.0	34.0	31.0	34.0
2	32.55970129140932	34.0	31.0	34.0	31.0	34.0
3	32.61235934868051	34.0	31.0	34.0	31.0	34.0
4	36.03348792813026	37.0	35.0	37.0	35.0	37.0
5	31.780715328467153	37.0	35.0	37.0	0.0	37.0
6	33.495427288040425	37.0	35.0	37.0	17.0	37.0
7	35.324752386299835	37.0	35.0	37.0	32.0	37.0
8	35.863385738349244	37.0	35.0	37.0	35.0	37.0
9	37.89932622122403	39.0	38.0	39.0	35.0	39.0
10	37.62031667602471	39.0	37.0	39.0	35.0	39.0
11	37.48960808534531	39.0	37.0	39.0	35.0	39.0
12	37.296946659180236	39.0	37.0	39.0	35.0	39.0
13	37.21542953396968	39.0	37.0	39.0	34.0	39.0
14	38.41661089275688	40.0	38.0	41.0	34.0	41.0
15	38.511630544637846	40.0	38.0	41.0	34.0	41.0
16	38.59890960134756	40.0	38.0	41.0	35.0	41.0
17	38.590516563728244	40.0	38.0	41.0	35.0	41.0
18	38.570907355418306	40.0	38.0	41.0	35.0	41.0
19	38.60349691184728	40.0	38.0	41.0	35.0	41.0
20	38.52483997754071	40.0	38.0	41.0	34.0	41.0
21	38.46546771476699	40.0	37.0	41.0	34.0	41.0
22	38.41874340258282	40.0	37.0	41.0	34.0	41.0
23	38.367825940482874	40.0	37.0	41.0	34.0	41.0
24	38.362687254351485	40.0	37.0	41.0	34.0	41.0
25	38.260997192588434	40.0	37.0	41.0	34.0	41.0
26	38.2012363840539	40.0	37.0	41.0	34.0	41.0
27	38.12799213924762	40.0	37.0	41.0	34.0	41.0
28	38.117371139809094	40.0	37.0	41.0	34.0	41.0
29	38.06164177428411	40.0	37.0	41.0	34.0	41.0
30	38.00745648512072	40.0	36.0	41.0	34.0	41.0
31	37.81730825379	40.0	36.0	41.0	34.0	41.0
32	37.871007299270076	40.0	36.0	41.0	34.0	41.0
33	37.844133632790566	40.0	36.0	41.0	33.0	41.0
34	37.865080291970806	40.0	36.0	41.0	34.0	41.0
35	37.80573161145424	40.0	36.0	41.0	33.0	41.0
36	37.780428972487364	40.0	36.0	41.0	33.0	41.0
37	37.748353733857385	40.0	36.0	41.0	33.0	41.0
38	37.733120718697364	40.0	36.0	41.0	33.0	41.0
39	37.730625491297026	40.0	36.0	41.0	33.0	41.0
40	37.69852554744526	40.0	36.0	41.0	33.0	41.0
41	37.66284222346996	40.0	36.0	41.0	33.0	41.0
42	37.661274564851205	40.0	36.0	41.0	33.0	41.0
43	37.60267040988209	40.0	35.0	41.0	33.0	41.0
44	37.52933520494104	40.0	35.0	41.0	33.0	41.0
45	37.480471645143176	39.0	35.0	41.0	33.0	41.0
46	37.40221673217294	39.0	35.0	41.0	33.0	41.0
47	37.35419988770354	39.0	35.0	41.0	33.0	41.0
48	37.35723862998316	39.0	35.0	41.0	33.0	41.0
49	37.33498820887142	39.0	35.0	41.0	33.0	41.0
50	37.25983380123526	39.0	35.0	41.0	33.0	41.0
51	36.64919708029197	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	4.0
12	3.0
13	15.0
14	17.0
15	23.0
16	21.0
17	41.0
18	76.0
19	133.0
20	249.0
21	476.0
22	767.0
23	1198.0
24	1700.0
25	2509.0
26	3544.0
27	4419.0
28	5649.0
29	7445.0
30	9990.0
31	13653.0
32	18758.0
33	29115.0
34	49248.0
35	77433.0
36	68151.0
37	107978.0
38	185662.0
39	301880.0
40	332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.051656372824258	4.544750140370579	58.80078607523863	15.602807411566536
2	18.25098259404829	4.537450870297586	55.40033688938798	21.811229646266142
3	20.09533969679955	4.9271195957327345	53.76103312745648	21.21650758001123
4	19.29298147108366	4.443571027512633	51.66108927568781	24.60235822571589
5	16.00393037619315	16.635710275126332	45.09500280741157	22.26535654126895
6	20.749241998877036	6.788321167883211	52.87827063447501	19.584166198764738
7	84.33329590117911	2.032453677709152	8.916788321167884	4.717462099943852
8	86.29904548006738	2.476698483997754	6.119595732734419	5.10466030320045
9	80.24536777091522	5.4561482313307135	8.34553621560921	5.952947782144862
10	37.42201010668164	35.61953958450309	14.24413250982594	12.714317798989333
11	27.71476698483998	25.117798989331835	29.212352610892754	17.955081414935428
12	26.843795620437955	22.062436833239754	31.597304884896126	19.496462661426165
13	22.871083660864684	24.348905109489053	33.11836047164514	19.661650758001123
14	18.191128579449746	30.337338573834927	30.20224592925323	21.2692869174621
15	16.014486243683322	26.41448624368332	38.03874227961819	19.53228523301516
16	18.7149915777653	25.424031443009543	34.941381246490735	20.919595732734418
17	19.294104435710274	23.964177428411006	30.57900056148231	26.162717574396403
18	19.60247052217855	24.950701852891637	32.09466591802359	23.352161706906234
19	23.6550252667041	25.561033127456483	29.524761370016844	21.259180235822573
20	26.708478382930938	26.123413812464907	28.254463784390794	18.913644020213365
21	24.507580011229646	26.815160022459295	28.658394160583946	20.01886580572712
22	22.114317798989333	25.209994385176866	28.521729365524983	24.153958450308817
23	21.023919146546884	27.439528354856822	28.46535654126895	23.071195957327344
24	21.461763054463784	25.233576642335763	30.1100505334082	23.19460976979225
25	20.921504772599665	27.29612577203818	28.321392476137003	23.460976979225155
26	20.103312745648513	29.66311061201572	27.624031443009546	22.609545199326224
27	20.706120157215047	28.140819764177426	30.145760808534533	21.007299270072995
28	19.501403705783268	27.471308253790006	32.10836608646828	20.91892195395845
29	21.28107804604155	25.86524424480629	31.07164514317799	21.78203256597417
30	22.57102751263335	26.77361033127457	29.50084222346996	21.154519932622122
31	23.893318360471646	29.184503088152724	25.644357102751265	21.27782144862437
32	23.693542953396967	29.088826501965187	25.962492981471087	21.25513756316676
33	22.563391353172374	28.619988770353732	27.694441325098257	21.12217855137563
34	22.41729365524986	26.139247613700167	28.2078607523863	23.235597978663673
35	21.559573273441888	25.880067377877598	28.555306007860754	24.005053340819764
36	23.732622122403143	26.120718697361035	29.01650758001123	21.130151600224593
37	23.044469399213924	27.092083099382368	30.001347557551938	19.862099943851767
38	20.56226838854576	28.80179674340258	27.301179112857945	23.33475575519371
39	21.205165637282423	27.755642897248737	28.615384615384613	22.423806850084222
40	23.51521617069062	25.72992700729927	28.34868051656373	22.40617630544638
41	20.693542953396967	25.417405951712517	28.27175743964065	25.617293655249863
42	22.425491297024145	24.056035934868053	28.344188658057273	25.174284110050532
43	22.623357664233577	24.704772599663112	29.475575519371137	23.19629421673217
44	21.754070746771475	26.299045480067377	28.47389107243122	23.47299270072993
45	21.5621560920831	26.650308815272318	26.904884896125775	24.88265019651881
46	23.135654126895002	26.568669286917462	27.631330713082537	22.664345873104995
47	20.469511510387424	25.94104435710275	30.499719258843346	23.08972487366648
48	20.339584503088155	24.59449747332959	30.54418865805727	24.521729365524987
49	21.559573273441888	24.122515440763618	31.365075800112297	22.9528354856822
50	20.61167883211679	24.90555867490174	29.322066254912972	25.160696238068503
51	19.48871420550253	25.26524424480629	26.768781583380125	28.477259966311063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	331.5
2	515.0
3	879.5
4	1244.0
5	978.5
6	713.0
7	859.0
8	1005.0
9	1105.5
10	1206.0
11	1255.5
12	1305.0
13	1304.0
14	1303.0
15	1445.5
16	1588.0
17	1766.0
18	1944.0
19	2285.0
20	2626.0
21	2782.0
22	2938.0
23	4263.0
24	5588.0
25	5795.5
26	8224.0
27	10445.0
28	12540.0
29	14635.0
30	16057.0
31	17479.0
32	20796.0
33	24113.0
34	26745.0
35	29377.0
36	30273.5
37	31170.0
38	34750.5
39	38331.0
40	43687.5
41	49044.0
42	55318.0
43	61592.0
44	65423.5
45	69255.0
46	80183.5
47	91112.0
48	98385.5
49	105659.0
50	98873.5
51	92088.0
52	76438.0
53	60788.0
54	50845.0
55	40902.0
56	35840.5
57	30779.0
58	30788.5
59	30798.0
60	27849.0
61	24900.0
62	20927.0
63	16954.0
64	13290.0
65	9626.0
66	7694.0
67	5762.0
68	4187.0
69	2612.0
70	2424.5
71	2237.0
72	1898.5
73	1560.0
74	1036.5
75	492.0
76	471.0
77	270.0
78	69.0
79	70.5
80	72.0
81	49.5
82	27.0
83	14.5
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	890500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.777400924499105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.9206074010794	15.911981122864669
2	12.516546357871539	5.9522188188238125
3	5.594011695829019	3.9903317660419124
4	3.1405304236475278	2.9869460399461722
5	2.0251604946158177	2.407652650846861
6	1.3538827151662511	1.9315087273954428
7	1.02069200002268	1.6988582033477677
8	0.7772943400324549	1.478563132743649
9	0.5940271524209049	1.2711979586835354
>10	4.1957345166129185	20.4079959992021
>50	1.3902258626029143	24.16535500742864
>100	0.4604148215205558	14.696919326860952
>500	0.008508692800174543	1.3652863593586675
>1k	0.0023635257778262617	1.7351848864558415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	3980	0.4469399213924761	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	3771	0.42346996069623805	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	3539	0.3974171813587872	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	2610	0.29309376754632227	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTG	1538	0.17271195957327345	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTG	909	0.10207748455923638	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44042672655811343	0.0
2	0.0	0.0	0.0	1.6622122403144302	0.0
3	0.0	0.0	0.0	2.365637282425604	0.0
4	0.0	0.0	0.0	3.1237507018528916	0.0
5	0.0	0.0	0.0	4.4139247613700165	0.0
6	0.0	0.0	0.0	5.21414935429534	0.0
7	0.0	0.0	0.0	5.971925884334643	0.0
8	0.0	0.0	0.0	7.278944413250983	0.0
9	0.0	0.0	0.0	7.9112857944974735	0.0
10	0.0	0.0	0.0	9.050758001122965	0.0
11	0.0	0.0	0.0	10.919371139809096	0.0
12	0.0	0.0	0.0	12.693430656934307	0.0
13	0.0	0.0	0.0	13.264907355418304	0.0
14	0.0	0.0	0.0	13.438517686692869	0.0
15	0.0	0.0	0.0	13.735878719820326	0.0
16	0.0	0.0	0.0	14.454463784390791	0.0
17	0.0	0.0	0.0	15.569567658618753	0.0
18	0.0	0.0	0.0	16.876024705221784	0.0
19	0.0	0.0	0.0	17.713307130825378	0.0
20	0.0	0.0	0.0	18.554070746771476	0.0
21	0.0	0.0	0.0	19.59910162829871	0.0
22	4.4918585064570465E-4	0.0	0.0	20.65266704098821	0.0
23	4.4918585064570465E-4	0.0	0.0	21.611454239191467	0.0
24	4.4918585064570465E-4	0.0	0.0	22.310050533408198	0.0
25	4.4918585064570465E-4	0.0	0.0	22.912296462661427	0.0
26	4.4918585064570465E-4	0.0	0.0	23.466254912970243	0.0
27	4.4918585064570465E-4	0.0	0.0	24.045929253228522	0.0
28	4.4918585064570465E-4	0.0	0.0	24.655699045480066	0.0
29	4.4918585064570465E-4	0.0	0.0	25.33239752947782	0.0
30	4.4918585064570465E-4	0.0	0.0	26.08276249298147	0.0
31	4.4918585064570465E-4	0.0	0.0	26.779786636720942	0.0
32	4.4918585064570465E-4	0.0	0.0	27.414261650758	0.0
33	4.4918585064570465E-4	0.0	0.0	28.010555867490176	0.0
34	4.4918585064570465E-4	0.0	0.0	28.620887142055025	0.0
35	4.4918585064570465E-4	0.0	0.0	29.285569904548005	0.0
36	4.4918585064570465E-4	0.0	0.0	29.92902863559798	0.0
37	4.4918585064570465E-4	0.0	0.0	30.570578326782705	0.0
38	5.614823133071309E-4	0.0	0.0	31.202695115103875	0.0
39	5.614823133071309E-4	0.0	0.0	31.8628860190904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.032431E-4	45.0	18
AGCGTCC	20	7.032431E-4	45.0	43
CTATGCG	20	7.032431E-4	45.0	1
CGGCGGA	25	3.8900962E-5	45.0	10
ATCTCGG	20	7.032431E-4	45.0	12
AAATCGA	20	7.032431E-4	45.0	23
GCGACAT	25	3.8900962E-5	45.0	37
GCGACAG	40	6.8121153E-9	45.0	36
CGAGTAC	25	3.8900962E-5	45.0	13
CGAAACT	25	3.8900962E-5	45.0	27
TCCGATC	20	7.032431E-4	45.0	16
CTATCGA	35	1.2115197E-7	45.0	32
GACACGT	20	7.032431E-4	45.0	21
TTTCGTC	25	3.8900962E-5	45.0	44
CCGGCAT	20	7.032431E-4	45.0	34
GACCGTA	20	7.032431E-4	45.0	9
TAGCGCG	20	7.032431E-4	45.0	2
GCCGGTC	25	3.8900962E-5	45.0	28
CGCGCAC	20	7.032431E-4	45.0	17
CCTACCG	25	3.8900962E-5	45.0	20
>>END_MODULE