##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540166
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38364502763965	33.0	31.0	34.0	31.0	34.0
2	32.54666343309279	34.0	31.0	34.0	31.0	34.0
3	32.58833580788128	34.0	31.0	34.0	31.0	34.0
4	36.01515274934002	37.0	35.0	37.0	35.0	37.0
5	31.920492959571686	37.0	35.0	37.0	0.0	37.0
6	33.58200627214597	37.0	35.0	37.0	17.0	37.0
7	35.29211760829079	37.0	35.0	37.0	32.0	37.0
8	35.79983375480871	37.0	35.0	37.0	35.0	37.0
9	37.81142648741313	39.0	38.0	39.0	35.0	39.0
10	37.595931621020206	39.0	37.0	39.0	35.0	39.0
11	37.520014217851546	39.0	37.0	39.0	35.0	39.0
12	37.17413165582432	39.0	37.0	39.0	34.0	39.0
13	37.00814564411681	39.0	35.0	39.0	33.0	39.0
14	38.16754479178623	40.0	37.0	41.0	33.0	41.0
15	38.31880570046985	40.0	37.0	41.0	34.0	41.0
16	38.403529655698435	40.0	37.0	41.0	34.0	41.0
17	38.395126683278846	40.0	37.0	41.0	34.0	41.0
18	38.40272805026603	40.0	37.0	41.0	34.0	41.0
19	38.41721063524916	40.0	37.0	41.0	34.0	41.0
20	38.32513338492241	40.0	37.0	41.0	34.0	41.0
21	38.266973485928396	40.0	37.0	41.0	34.0	41.0
22	38.2236979002751	40.0	36.0	41.0	34.0	41.0
23	38.191868795888674	40.0	36.0	41.0	34.0	41.0
24	38.16024333260516	40.0	36.0	41.0	34.0	41.0
25	38.04369397555566	40.0	36.0	41.0	34.0	41.0
26	37.96363895543222	40.0	36.0	41.0	34.0	41.0
27	37.87879281554189	40.0	36.0	41.0	34.0	41.0
28	37.84724695741679	40.0	36.0	41.0	34.0	41.0
29	37.801733170914126	40.0	36.0	41.0	34.0	41.0
30	37.78137276318761	40.0	36.0	41.0	34.0	41.0
31	37.58350210861106	40.0	35.0	41.0	33.0	41.0
32	37.606017409463014	40.0	35.0	41.0	33.0	41.0
33	37.5924882350981	40.0	35.0	41.0	33.0	41.0
34	37.56575015828467	40.0	35.0	41.0	33.0	41.0
35	37.49963159473199	39.0	35.0	41.0	33.0	41.0
36	37.47690709892885	39.0	35.0	41.0	33.0	41.0
37	37.42172961645124	39.0	35.0	41.0	33.0	41.0
38	37.46789505448325	39.0	35.0	41.0	33.0	41.0
39	37.45581173194907	39.0	35.0	41.0	33.0	41.0
40	37.371037792086135	39.0	35.0	41.0	33.0	41.0
41	37.30804789638741	39.0	35.0	41.0	33.0	41.0
42	37.300509473013854	39.0	35.0	41.0	33.0	41.0
43	37.234429786399	39.0	35.0	41.0	33.0	41.0
44	37.17918565774225	39.0	35.0	41.0	33.0	41.0
45	37.1516830011515	39.0	35.0	41.0	33.0	41.0
46	37.06326388554629	39.0	35.0	41.0	33.0	41.0
47	37.01697441156978	39.0	35.0	41.0	33.0	41.0
48	37.00325270379846	39.0	35.0	41.0	33.0	41.0
49	37.01049862449691	39.0	35.0	41.0	33.0	41.0
50	36.93035289151853	39.0	35.0	41.0	33.0	41.0
51	36.323502404816296	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	6.0
11	5.0
12	5.0
13	4.0
14	11.0
15	13.0
16	21.0
17	33.0
18	49.0
19	102.0
20	170.0
21	332.0
22	557.0
23	828.0
24	1273.0
25	1742.0
26	2288.0
27	2903.0
28	3771.0
29	4674.0
30	6180.0
31	8658.0
32	12076.0
33	19710.0
34	34835.0
35	57161.0
36	42730.0
37	65459.0
38	109461.0
39	164908.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.43384441079224	5.21932146784507	56.24937519207058	16.09745892929211
2	19.90665832355239	5.33243484410348	51.820551460106714	22.940355372237423
3	20.764542751672636	5.150083492852197	51.18315480796644	22.90221894750873
4	18.299189508410375	4.812779775106171	51.28034715254199	25.607683563941453
5	17.39965121832918	16.499557543421837	43.5706801242581	22.530111113990884
6	21.869018042601716	6.395996786173138	53.13237041946365	18.602614751761497
7	82.0560716520477	2.961682149561431	9.639073914315228	5.343172284075636
8	83.32531110806678	3.6461013836487304	7.276651992165371	5.751935516119119
9	77.11296157107259	6.300285467800639	10.108744348959393	6.478008612167371
10	39.77258842652074	28.269087650833264	16.796503297134585	15.161820625511416
11	34.127286797021654	24.17553122558621	23.350599630483963	18.34658234690817
12	30.132033486002452	21.1827475257606	28.121540415353795	20.563678572883152
13	24.52597905088436	26.84915377865323	28.72728013240374	19.89758703805867
14	18.394160313681347	31.270942636152586	28.55659186250153	21.778305187664532
15	15.834576778249648	24.38546668986941	39.176845636341426	20.60311089553952
16	19.168366761328926	22.06247709037592	35.18344360807604	23.585712540219117
17	17.945039117604587	22.992746674170533	29.693279473347083	29.3689347348778
18	21.0078013055246	22.26482229536846	32.25193736740187	24.47543903170507
19	24.440449787657865	23.695863864071416	29.098277196269294	22.76540915200142
20	27.585594058122876	25.454027095374386	26.68105730460636	20.27932154189638
21	24.809595568769602	25.717094374692223	27.728142830167023	21.745167226371155
22	22.988303595561366	25.008608464805267	27.139620042727607	24.863467896905767
23	21.945661148609872	25.802993894469477	26.48111876719379	25.770226189726863
24	23.26118267347445	22.769852230610592	29.258968539300884	24.70999655661408
25	20.79823609779216	26.303025366276295	27.504693001780932	25.39404553415061
26	19.70542388821214	29.970601629869336	25.973126779545545	24.350847702372974
27	20.82119200393953	29.841567221928074	27.127401576552394	22.209839197580003
28	19.052106204389023	28.634901122988122	29.178622867785087	23.134369804837775
29	19.671730542092618	27.10407541385426	28.344434858913743	24.879759185139385
30	21.317706038514086	27.62095355872084	25.872046741186967	25.189293661578105
31	23.234709330094823	29.323578307409203	22.558435740124335	24.883276622371643
32	23.668464879314875	28.047859361751758	23.856740335378383	24.426935423554983
33	22.535109577426198	28.078405527189794	23.7278910557125	25.658593839671507
34	19.643035659408405	28.49031593991477	26.39299770811195	25.47365069256488
35	19.681912597238625	27.490252996301134	25.717094374692223	27.11074003176801
36	23.64199153593525	28.64415753675722	23.674759240677865	24.039091686629664
37	21.143500331379613	29.951903674055753	26.492781848542858	22.411814146021776
38	21.449887627136842	27.631135613866846	23.57478997197158	27.344186787024725
39	20.646616040254294	26.717897831407385	26.280995101505834	26.354491026832495
40	22.12949352606421	25.49623634216148	25.888338029420588	26.485932102353722
41	17.987803749217832	27.990839852934098	25.657483070019214	28.363873327828852
42	21.246801909042777	27.665569473087903	25.292039854415123	25.795588763454198
43	22.633042435103285	26.96615484869466	25.315736273664026	25.085066442538036
44	21.12739417142138	26.96226715491164	25.24538752901886	26.664951144648125
45	21.086665950837336	26.778990162283446	23.86932905810436	28.26501482877486
46	22.474757759651663	26.618483947527245	25.785776964858954	25.120981327962145
47	18.839949200801236	27.94307675788554	28.006390628066185	25.21058341324704
48	19.549175623789726	26.81897786976596	26.887105075106543	26.744741431337776
49	21.356397848068926	24.282165112206247	28.996086388258423	25.3653506514664
50	20.555903185317106	24.770718630939378	26.524994168459326	28.14838401528419
51	18.856795873861	25.029157703372668	25.13856851412344	30.9754779086429
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	173.0
1	286.0
2	399.0
3	592.5
4	786.0
5	622.0
6	458.0
7	553.0
8	648.0
9	696.5
10	745.0
11	759.0
12	773.0
13	891.5
14	1010.0
15	1036.5
16	1063.0
17	1179.0
18	1295.0
19	1234.0
20	1173.0
21	1415.5
22	1658.0
23	1741.5
24	1825.0
25	2153.5
26	2702.0
27	2922.0
28	3724.0
29	4526.0
30	5221.0
31	5916.0
32	7855.0
33	9794.0
34	11892.5
35	13991.0
36	14387.0
37	14783.0
38	15878.0
39	16973.0
40	21591.0
41	26209.0
42	30955.0
43	35701.0
44	39284.5
45	42868.0
46	48664.0
47	54460.0
48	59333.0
49	64206.0
50	60959.5
51	57713.0
52	48849.0
53	39985.0
54	34250.5
55	28516.0
56	26775.5
57	25035.0
58	23552.0
59	22069.0
60	20371.5
61	18674.0
62	16296.5
63	13919.0
64	12343.0
65	10767.0
66	8402.5
67	6038.0
68	5230.0
69	4422.0
70	3396.0
71	2370.0
72	2195.5
73	2021.0
74	1577.5
75	714.5
76	295.0
77	250.5
78	206.0
79	166.5
80	127.0
81	68.0
82	9.0
83	15.0
84	21.0
85	14.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.733003605150305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80433096168494	16.770047621201577
2	12.412014172347217	6.139727825436808
3	5.413945618463318	4.017094094986228
4	2.9856675190787905	2.9537810205262356
5	2.0004101178880624	2.4738075328752296
6	1.3269393746135965	1.9691517801680385
7	0.9622408167926924	1.66593739135295
8	0.7495524321632135	1.4830946405553551
9	0.5904140374199159	1.3142441264434315
>10	3.9405999359208534	20.588323459056674
>50	1.464310813317088	26.27425274862564
>100	0.3390719711164568	11.514049494917167
>500	0.00750159228133754	1.1792725932143078
>1k	0.003000636912535016	1.6572156706403707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	2544	0.470966332571839	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	2513	0.46522735603499665	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	2081	0.3852519410699674	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	1794	0.3321201260353299	No Hit
TGTTTGGGGTGGTATCAAACCAAGCAGAGACTTTGGTGCTGGTTGGAAACA	893	0.16531954991613687	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	829	0.1534713402916881	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC	766	0.14180825894262133	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG	624	0.11552004383837561	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	566	0.1047826038662189	No Hit
TTACCTGGGTACCTTGATTATGTTAGTTGGCACTGGATTCCAAATTCTACC	564	0.10441234731545489	No Hit
TTTCTTGGGGGACAATAGGAATTGAACCTACACTTAAGAATTCAAAATTCT	548	0.1014502949093427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4783714635871195	0.0
2	0.0	0.0	0.0	1.7511283568384535	0.0
3	0.0	0.0	0.0	2.4361029757518984	0.0
4	0.0	0.0	0.0	3.1986463420504068	0.0
5	0.0	0.0	0.0	4.510835557958109	0.0
6	0.0	0.0	0.0	5.345208695104838	0.0
7	0.0	0.0	0.0	6.145518229581277	0.0
8	0.0	0.0	0.0	7.440860772429216	0.0
9	0.0	0.0	0.0	8.125835391342662	0.0
10	0.0	0.0	0.0	9.372859454315895	0.0
11	0.0	0.0	0.0	11.181562704798155	0.0
12	0.0	0.0	0.0	12.988229544251212	0.0
13	0.0	0.0	0.0	13.528804108366687	0.0
14	0.0	0.0	0.0	13.733555980939192	0.0
15	0.0	0.0	0.0	14.073081237989802	0.0
16	1.851282753820122E-4	0.0	0.0	14.850064609768108	0.0
17	1.851282753820122E-4	0.0	0.0	16.000821969542695	0.0
18	1.851282753820122E-4	0.0	0.0	17.334115808843947	0.0
19	1.851282753820122E-4	0.0	0.0	18.16682279151224	0.0
20	1.851282753820122E-4	0.0	0.0	18.986941051454554	0.0
21	1.851282753820122E-4	0.0	0.0	19.970527578559185	0.0
22	1.851282753820122E-4	0.0	0.0	20.944487435343948	0.0
23	1.851282753820122E-4	0.0	0.0	21.87734881499391	0.0
24	1.851282753820122E-4	0.0	0.0	22.58787113591007	0.0
25	1.851282753820122E-4	0.0	0.0	23.21397496325204	0.0
26	1.851282753820122E-4	0.0	0.0	23.74603362669994	0.0
27	1.851282753820122E-4	0.0	0.0	24.31011948178893	0.0
28	1.851282753820122E-4	0.0	0.0	24.899012525779114	0.0
29	1.851282753820122E-4	0.0	0.0	25.515489682801213	0.0
30	1.851282753820122E-4	0.0	0.0	26.191948401047085	0.0
31	1.851282753820122E-4	0.0	0.0	26.805648633938457	0.0
32	1.851282753820122E-4	0.0	0.0	27.42879040887431	0.0
33	1.851282753820122E-4	0.0	0.0	28.03601115212731	0.0
34	1.851282753820122E-4	0.0	0.0	28.618424706479118	0.0
35	1.851282753820122E-4	0.0	0.0	29.179363380886617	0.0
36	1.851282753820122E-4	0.0	0.0	29.74122769667102	0.0
37	3.702565507640244E-4	0.0	0.0	30.331786895139643	0.0
38	3.702565507640244E-4	0.0	0.0	30.89179992817023	0.0
39	3.702565507640244E-4	0.0	0.0	31.538267865804215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.029961E-4	45.000004	1
GTCATAG	20	7.029961E-4	45.000004	45
CGGGTGC	55	1.8189894E-12	45.000004	6
AGCGTAA	20	7.029961E-4	45.000004	11
TACCCTC	20	7.029961E-4	45.000004	23
ACGCCCG	20	7.029961E-4	45.000004	37
AATTTCG	20	7.029961E-4	45.000004	26
AATCACG	20	7.029961E-4	45.000004	21
TACTTAG	20	7.029961E-4	45.000004	29
GATCGAG	20	7.029961E-4	45.000004	12
GTTTCGT	20	7.029961E-4	45.000004	36
CAACGGT	20	7.029961E-4	45.000004	42
TTAGCGA	20	7.029961E-4	45.000004	27
CCCTACC	20	7.029961E-4	45.000004	21
CGTTGTG	20	7.029961E-4	45.000004	13
GACGTCA	30	2.1633568E-6	45.000004	10
GAGTAGA	20	7.029961E-4	45.000004	32
CGCATCG	20	7.029961E-4	45.000004	27
TCTTACG	55	1.8189894E-12	45.000004	1
TTCACGT	30	2.1633568E-6	45.000004	17
>>END_MODULE