SRR2935339.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2342500
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	6697	0.2858911419423693	RNA PCR Primer, Index 27 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	6015	0.2567769477054429	Illumina PCR Primer Index 6 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	5999	0.256093916755603	RNA PCR Primer, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	5262	0.2246318036286019	Illumina PCR Primer Index 6 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2591	0.11060832443970116	No Hit
TCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTG	2442	0.10424759871931696	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.0347876E-4	45.000004	22
GTCGAAC	40	6.8193913E-9	45.000004	13
GCACGGT	20	7.0347876E-4	45.000004	3
CCCGATA	20	7.0347876E-4	45.000004	28
CGGACGT	20	7.0347876E-4	45.000004	41
TATTACG	60	0.0	44.999996	1
TTACGGG	1595	0.0	42.460815	3
TTATGGG	4650	0.0	41.32258	3
TATGGGA	3165	0.0	41.090046	4
CTTACGG	420	0.0	40.714287	2
TTTACGG	960	0.0	40.54687	2
TCTACGG	450	0.0	40.000004	2
CGAATCG	45	1.9297659E-8	40.0	36
TACGGGA	1025	0.0	39.951218	4
TATGGGC	1465	0.0	39.77816	4
TACGGGT	300	0.0	39.75	4
CGTATGG	385	0.0	39.74026	2
TATTGCG	80	0.0	39.375004	1
CTATGGG	2425	0.0	39.340206	3
TACGGGG	1205	0.0	39.211617	4