##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2342500
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40068772678762	33.0	31.0	34.0	31.0	34.0
2	32.54998591248666	34.0	31.0	34.0	31.0	34.0
3	32.61257545357524	34.0	31.0	34.0	31.0	34.0
4	36.030282177161155	37.0	35.0	37.0	35.0	37.0
5	31.842022625400215	37.0	35.0	37.0	0.0	37.0
6	33.51431931696905	37.0	35.0	37.0	17.0	37.0
7	35.26556499466382	37.0	35.0	37.0	32.0	37.0
8	35.79583521878335	37.0	35.0	37.0	35.0	37.0
9	37.830740661686235	39.0	38.0	39.0	35.0	39.0
10	37.57305485592316	39.0	37.0	39.0	35.0	39.0
11	37.504084951974384	39.0	37.0	39.0	35.0	39.0
12	37.31493020277481	39.0	37.0	39.0	35.0	39.0
13	37.29403287086446	39.0	37.0	39.0	34.0	39.0
14	38.53825997865528	40.0	38.0	41.0	35.0	41.0
15	38.60327129135539	40.0	38.0	41.0	35.0	41.0
16	38.659619637139805	40.0	38.0	41.0	35.0	41.0
17	38.63446318036286	40.0	38.0	41.0	35.0	41.0
18	38.59389498399146	40.0	38.0	41.0	34.0	41.0
19	38.62555005336179	40.0	38.0	41.0	34.0	41.0
20	38.57047385272145	40.0	38.0	41.0	34.0	41.0
21	38.516450800426895	40.0	38.0	41.0	34.0	41.0
22	38.484954108858055	40.0	38.0	41.0	34.0	41.0
23	38.43324610458912	40.0	38.0	41.0	34.0	41.0
24	38.410899039487724	40.0	38.0	41.0	34.0	41.0
25	38.312495624332975	40.0	37.0	41.0	34.0	41.0
26	38.26828772678762	40.0	37.0	41.0	34.0	41.0
27	38.22602177161153	40.0	37.0	41.0	34.0	41.0
28	38.19838676627535	40.0	37.0	41.0	34.0	41.0
29	38.15417972251868	40.0	37.0	41.0	34.0	41.0
30	38.0971159018143	40.0	37.0	41.0	34.0	41.0
31	37.91801579509072	40.0	37.0	41.0	34.0	41.0
32	37.96029882604056	40.0	37.0	41.0	34.0	41.0
33	37.951682390608326	40.0	37.0	41.0	34.0	41.0
34	37.8918522945571	40.0	37.0	41.0	33.0	41.0
35	37.82436029882604	40.0	37.0	41.0	33.0	41.0
36	37.83209178228388	40.0	37.0	41.0	33.0	41.0
37	37.831117609391676	40.0	36.0	41.0	33.0	41.0
38	37.82540106723586	40.0	36.0	41.0	33.0	41.0
39	37.79167854855923	40.0	36.0	41.0	33.0	41.0
40	37.67969946638207	40.0	36.0	41.0	33.0	41.0
41	37.607162006403414	40.0	36.0	41.0	33.0	41.0
42	37.617479188900745	40.0	36.0	41.0	33.0	41.0
43	37.577018996798294	40.0	36.0	41.0	33.0	41.0
44	37.52608110992529	40.0	35.0	41.0	33.0	41.0
45	37.48209306296692	40.0	35.0	41.0	33.0	41.0
46	37.38071419423693	39.0	35.0	41.0	33.0	41.0
47	37.32441067235859	39.0	35.0	41.0	33.0	41.0
48	37.2943325506937	39.0	35.0	41.0	33.0	41.0
49	37.256953681963715	39.0	35.0	41.0	33.0	41.0
50	37.1869613660619	39.0	35.0	41.0	33.0	41.0
51	36.56973703308431	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	7.0
11	25.0
12	19.0
13	24.0
14	31.0
15	71.0
16	106.0
17	158.0
18	284.0
19	491.0
20	815.0
21	1402.0
22	2085.0
23	3306.0
24	5041.0
25	7332.0
26	9902.0
27	12737.0
28	15947.0
29	20434.0
30	26457.0
31	35462.0
32	48742.0
33	73896.0
34	125464.0
35	175258.0
36	183044.0
37	292651.0
38	490837.0
39	809543.0
40	916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.57203842049093	4.502326574172892	55.718932764140874	16.206702241195302
2	19.449775880469584	4.735410885805763	53.49600853788687	22.31880469583778
3	21.16187833511206	4.672614727854856	51.89976520811099	22.26574172892209
4	19.26347918890075	4.981856990394877	50.77596584845251	24.97869797225187
5	16.270480256136608	17.215581643543224	43.47214514407684	23.041792956243327
6	21.234663820704373	6.9744290288153685	52.727086446104586	19.063820704375665
7	83.86932764140876	2.686147278548559	8.635602988260406	4.808922091782284
8	86.09442902881537	3.2690715048025614	6.067491995731056	4.569007470651014
9	80.38480256136606	5.350693703308431	8.47479188900747	5.789711846318036
10	37.23385272145144	28.964994663820704	17.14403415154749	16.65711846318036
11	28.995005336179297	27.01767342582711	24.459167556029882	19.528153681963715
12	27.36947705442903	23.10091782283885	28.718676627534684	20.81092849519744
13	24.31812166488794	22.939594450373534	30.690117395944505	22.052166488794022
14	20.66608324439701	25.74715048025614	30.124653148345786	23.462113127001068
15	19.15086446104589	25.60614727854856	33.536947705442905	21.706040554962648
16	21.36934898612593	24.81169690501601	31.411483457844184	22.407470651013874
17	21.291398078975455	24.524439701173957	29.407129135538955	24.777033084311633
18	21.989626467449305	24.98266808964781	30.321237993596583	22.7064674493063
19	23.427065101387406	26.136862326574175	28.631462113127	21.80461045891142
20	26.636627534685164	26.324482390608324	27.06872998932764	19.97016008537887
21	25.753639274279617	26.676542155816435	27.292508004268946	20.277310565635005
22	23.785058697972254	25.02544290288154	27.734429028815367	23.455069370330843
23	23.474066168623267	25.68828175026681	27.866979722518675	22.970672358591248
24	24.289306296691567	23.647470651013876	28.709199573105654	23.3540234791889
25	22.778100320170758	26.478762006403418	26.34676627534685	24.396371398078976
26	22.2936606189968	27.3584204909285	26.591803628601923	23.756115261472786
27	21.54744930629669	26.650117395944505	27.568580576307365	24.23385272145144
28	20.4978014941302	26.451184631803628	28.597908217716117	24.45310565635005
29	22.26586979722519	24.873596584845252	28.009477054429027	24.851056563500535
30	21.961408751334044	25.56546424759872	27.93805763073639	24.53506937033084
31	22.09306296691569	27.11210245464248	26.245933831376732	24.5489007470651
32	23.15043756670224	26.492508004268945	26.746125933831376	23.610928495197438
33	22.252636072572038	26.322347918890078	27.925848452508006	23.49916755602988
34	21.401494130202774	25.635176093916755	28.270096051227323	24.693233724653147
35	21.30932764140875	24.724909284951973	28.436029882604057	25.52973319103522
36	21.20623265741729	26.963201707577372	27.764226254002132	24.066339381003203
37	20.927897545357524	26.831334044823908	27.818271077908218	24.422497331910353
38	20.38983991462113	26.692251867662755	27.583479188900746	25.334429028815368
39	21.516926360725723	25.23419423692636	29.17016008537887	24.07871931696905
40	22.111291355389543	25.401750266808964	30.120811099252936	22.36614727854856
41	19.433639274279617	26.637566702241195	29.455837780149412	24.472956243329776
42	20.851867662753467	25.067748132337247	28.520597652081108	25.55978655282817
43	20.824162219850585	24.72879402347919	28.56836712913554	25.878676627534684
44	21.22066168623266	24.54249733191035	27.552614727854856	26.684226254002137
45	21.56034151547492	23.849775880469583	27.383735325506937	27.20614727854856
46	21.274322305229457	25.231974386339378	28.40537886872999	25.088324439701175
47	19.418441835645677	25.607556029882605	30.259124866595517	24.7148772678762
48	19.657673425827106	23.18032017075774	30.943393810032017	26.21861259338314
49	20.428601921024548	23.223094983991462	30.730757737459978	25.617545357524012
50	20.110907150480255	23.166189967982927	29.914151547491997	26.80875133404482
51	19.280256136606187	23.483372465314833	29.177075773745997	28.059295624332975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	364.0
1	1218.5
2	2073.0
3	3278.5
4	4484.0
5	3350.0
6	2216.0
7	2355.0
8	2494.0
9	2686.5
10	2879.0
11	2902.0
12	2925.0
13	3004.5
14	3084.0
15	3168.5
16	3253.0
17	3635.0
18	4017.0
19	4601.5
20	5186.0
21	5928.0
22	6670.0
23	7791.0
24	8912.0
25	11919.5
26	20796.5
27	26666.0
28	29527.0
29	32388.0
30	37854.0
31	43320.0
32	49864.5
33	56409.0
34	65465.0
35	74521.0
36	76496.5
37	78472.0
38	84642.5
39	90813.0
40	100496.5
41	110180.0
42	120036.0
43	129892.0
44	145234.0
45	160576.0
46	178439.0
47	196302.0
48	214925.0
49	233548.0
50	226353.5
51	219159.0
52	193267.5
53	167376.0
54	153165.0
55	138954.0
56	130194.0
57	121434.0
58	114922.0
59	108410.0
60	103058.0
61	97706.0
62	84380.0
63	71054.0
64	58302.5
65	45551.0
66	36944.0
67	28337.0
68	22856.5
69	17376.0
70	14931.5
71	12487.0
72	9812.5
73	7138.0
74	6299.5
75	4265.5
76	3070.0
77	2184.5
78	1299.0
79	892.0
80	485.0
81	417.5
82	350.0
83	220.0
84	90.0
85	132.5
86	175.0
87	95.0
88	15.0
89	8.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2342500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.672285829965368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.49646649268675	15.529714666648395
2	12.918850429124445	5.857997390473603
3	5.377942895504881	3.657907755123547
4	2.750214843137283	2.4941462806968735
5	1.6302769227848897	1.84810521876877
6	1.0244098806258761	1.3935428172354338
7	0.6834529410820005	1.0846808302079118
8	0.49549503468804557	0.8987204043020778
9	0.3621047058367249	0.7388767251995179
>10	4.001516961769128	22.55999664188259
>50	1.9537733296614184	31.528253470849897
>100	0.296691093438288	9.571400950165918
>500	0.005733143034560161	0.9120660682662288
>1k	0.0022523061921486346	0.8117002699463027
>5k	8.190204335085943E-4	1.1128905102329263
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	6697	0.2858911419423693	RNA PCR Primer, Index 27 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	6015	0.2567769477054429	Illumina PCR Primer Index 6 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	5999	0.256093916755603	RNA PCR Primer, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	5262	0.2246318036286019	Illumina PCR Primer Index 6 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2591	0.11060832443970116	No Hit
TCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTG	2442	0.10424759871931696	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7075773745997867E-4	0.0	0.0	0.29801494130202777	0.0
2	1.7075773745997867E-4	0.0	0.0	1.0866168623265742	0.0
3	1.7075773745997867E-4	0.0	0.0	1.544845250800427	0.0
4	1.7075773745997867E-4	0.0	0.0	2.0665101387406617	0.0
5	1.7075773745997867E-4	0.0	0.0	2.9894983991462114	0.0
6	1.7075773745997867E-4	0.0	0.0	3.657417289220918	0.0
7	1.7075773745997867E-4	0.0	0.0	4.231077908217716	0.0
8	1.7075773745997867E-4	0.0	0.0	5.1156883671291355	0.0
9	1.7075773745997867E-4	0.0	0.0	5.526830309498399	0.0
10	1.7075773745997867E-4	0.0	0.0	6.353895410885806	0.0
11	2.1344717182497332E-4	0.0	0.0	7.766659551760939	0.0
12	2.1344717182497332E-4	0.0	0.0	8.970416221985058	0.0
13	2.1344717182497332E-4	0.0	0.0	9.35257203842049	0.0
14	2.1344717182497332E-4	0.0	0.0	9.495667022411952	0.0
15	2.1344717182497332E-4	0.0	0.0	9.738057630736392	0.0
16	2.1344717182497332E-4	0.0	0.0	10.300704375667022	0.0
17	2.1344717182497332E-4	0.0	0.0	11.044823906083245	0.0
18	2.1344717182497332E-4	0.0	0.0	11.852721451440768	0.0
19	2.1344717182497332E-4	0.0	0.0	12.427363927427962	0.0
20	2.1344717182497332E-4	0.0	0.0	13.027748132337246	0.0
21	2.1344717182497332E-4	0.0	0.0	13.846830309498399	0.0
22	2.1344717182497332E-4	0.0	0.0	14.699679829242262	0.0
23	2.56136606189968E-4	0.0	0.0	15.506382070437567	0.0
24	2.56136606189968E-4	0.0	0.0	16.13152614727855	0.0
25	2.9882604055496263E-4	0.0	0.0	16.717566702241196	0.0
26	2.9882604055496263E-4	0.0	0.0	17.204055496264676	0.0
27	2.9882604055496263E-4	0.0	0.0	17.689903948772677	0.0
28	2.9882604055496263E-4	0.0	0.0	18.228601921024545	0.0
29	2.9882604055496263E-4	0.0	0.0	18.770202774813235	0.0
30	2.9882604055496263E-4	0.0	0.0	19.38582710779082	0.0
31	2.9882604055496263E-4	0.0	0.0	19.9489007470651	0.0
32	2.9882604055496263E-4	0.0	0.0	20.49272145144077	0.0
33	3.4151547491995734E-4	0.0	0.0	21.03282817502668	0.0
34	3.4151547491995734E-4	0.0	0.0	21.540661686232657	0.0
35	3.4151547491995734E-4	0.0	0.0	22.092209178228387	0.0
36	3.4151547491995734E-4	0.0	0.0	22.645933831376734	0.0
37	3.4151547491995734E-4	0.0	0.0	23.20076840981857	0.0
38	3.4151547491995734E-4	0.0	0.0	23.781173959445038	0.0
39	3.4151547491995734E-4	0.0	0.0	24.332166488794023	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.0347876E-4	45.000004	22
GTCGAAC	40	6.8193913E-9	45.000004	13
GCACGGT	20	7.0347876E-4	45.000004	3
CCCGATA	20	7.0347876E-4	45.000004	28
CGGACGT	20	7.0347876E-4	45.000004	41
TATTACG	60	0.0	44.999996	1
TTACGGG	1595	0.0	42.460815	3
TTATGGG	4650	0.0	41.32258	3
TATGGGA	3165	0.0	41.090046	4
CTTACGG	420	0.0	40.714287	2
TTTACGG	960	0.0	40.54687	2
TCTACGG	450	0.0	40.000004	2
CGAATCG	45	1.9297659E-8	40.0	36
TACGGGA	1025	0.0	39.951218	4
TATGGGC	1465	0.0	39.77816	4
TACGGGT	300	0.0	39.75	4
CGTATGG	385	0.0	39.74026	2
TATTGCG	80	0.0	39.375004	1
CTATGGG	2425	0.0	39.340206	3
TACGGGG	1205	0.0	39.211617	4
>>END_MODULE