##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935338.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2950406
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27358980425067	33.0	31.0	34.0	30.0	34.0
2	32.415785149569246	34.0	31.0	34.0	30.0	34.0
3	32.49580091689076	34.0	31.0	34.0	30.0	34.0
4	35.99623475548789	37.0	35.0	37.0	35.0	37.0
5	31.97953569779888	37.0	35.0	37.0	0.0	37.0
6	33.32526574308756	37.0	35.0	37.0	17.0	37.0
7	35.18140689789812	37.0	35.0	37.0	32.0	37.0
8	35.57026388910543	37.0	35.0	37.0	33.0	37.0
9	37.62702319612962	39.0	37.0	39.0	35.0	39.0
10	37.444111081661305	39.0	37.0	39.0	35.0	39.0
11	37.35722134513013	39.0	37.0	39.0	34.0	39.0
12	37.2567250066601	39.0	37.0	39.0	34.0	39.0
13	37.18155264055184	39.0	37.0	39.0	33.0	39.0
14	38.45053053715319	40.0	38.0	41.0	34.0	41.0
15	38.46049763998582	40.0	38.0	41.0	34.0	41.0
16	38.59191412978417	40.0	38.0	41.0	34.0	41.0
17	38.454205285645436	40.0	38.0	41.0	34.0	41.0
18	38.476606948331856	40.0	38.0	41.0	34.0	41.0
19	38.473091161013095	40.0	38.0	41.0	34.0	41.0
20	38.178486960777605	40.0	37.0	41.0	33.0	41.0
21	38.30799151032095	40.0	37.0	41.0	34.0	41.0
22	38.312963368431326	40.0	38.0	41.0	34.0	41.0
23	37.992417992642366	40.0	37.0	41.0	33.0	41.0
24	38.092493711035026	40.0	37.0	41.0	33.0	41.0
25	38.15255730906187	40.0	37.0	41.0	34.0	41.0
26	38.108987373263204	40.0	37.0	41.0	33.0	41.0
27	37.872954773004125	40.0	37.0	41.0	33.0	41.0
28	37.820689762697064	40.0	37.0	41.0	33.0	41.0
29	37.80099891336989	40.0	37.0	41.0	33.0	41.0
30	37.7728221132956	40.0	37.0	41.0	33.0	41.0
31	37.5536309240152	40.0	36.0	41.0	32.0	41.0
32	37.36659293670092	40.0	36.0	41.0	31.0	41.0
33	37.28915105243143	40.0	36.0	41.0	31.0	41.0
34	37.1287812592572	40.0	36.0	41.0	31.0	41.0
35	37.08218665498918	40.0	36.0	41.0	31.0	41.0
36	36.97856633968342	40.0	36.0	41.0	31.0	41.0
37	36.92948767051043	40.0	36.0	41.0	31.0	41.0
38	36.912147006208635	40.0	35.0	41.0	31.0	41.0
39	36.92171009684769	40.0	36.0	41.0	31.0	41.0
40	36.961602233726474	40.0	35.0	41.0	31.0	41.0
41	36.794858741474904	40.0	35.0	41.0	30.0	41.0
42	36.667304093063805	40.0	35.0	41.0	30.0	41.0
43	36.63083860322952	40.0	35.0	41.0	31.0	41.0
44	36.72933996202556	39.0	35.0	41.0	31.0	41.0
45	36.590484157095666	39.0	35.0	41.0	30.0	41.0
46	36.5119105641732	39.0	35.0	41.0	30.0	41.0
47	36.386305139021545	39.0	35.0	41.0	30.0	41.0
48	36.433988407019235	39.0	35.0	41.0	30.0	41.0
49	36.42190193485236	39.0	35.0	41.0	31.0	41.0
50	36.29759870336489	39.0	35.0	41.0	30.0	41.0
51	35.825774486629975	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	18.0
11	15.0
12	22.0
13	27.0
14	33.0
15	39.0
16	82.0
17	173.0
18	421.0
19	1208.0
20	3497.0
21	7740.0
22	13542.0
23	19336.0
24	24338.0
25	26223.0
26	25418.0
27	23787.0
28	24673.0
29	29214.0
30	37225.0
31	49714.0
32	65670.0
33	91902.0
34	143253.0
35	195701.0
36	225212.0
37	354695.0
38	593907.0
39	992088.0
40	1217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.314741089870346	3.7524666096801593	49.89296388361466	15.039828416834835
2	16.52084492778282	15.813620227182293	47.92672601669059	19.738808828344304
3	17.476340544318308	15.652794903481078	47.55667525079599	19.314189301404618
4	16.276742929617143	4.003245655004769	57.66362324371629	22.056388171661798
5	23.528829591588412	15.407777777024586	40.00157944364267	21.06181318774433
6	19.00118153230437	15.188452029991806	48.63950927431682	17.170857163387005
7	76.1669071985347	1.9331237802526162	17.488779510345356	4.411189510867318
8	77.25248660692799	11.342201717322972	6.88078183138185	4.524529844367182
9	72.51612151005659	5.044288819911564	7.697313522274561	14.742276147757291
10	38.766325719239994	33.35147772882783	14.36626010115218	13.515936450779995
11	29.809355051474274	22.602584186718712	29.151377810375926	18.43668295143109
12	23.264154153699526	20.353808933414587	30.49404048120835	25.88799643167754
13	25.443718593305466	20.46301424278557	35.26301126014522	18.830255903763753
14	19.145873483174856	29.512175612441133	28.834438378989198	22.50751252539481
15	16.29704522021715	23.47717568361778	35.831916014270575	24.393863081894494
16	18.020469047310776	24.63759225001576	29.409477882027087	27.932460820646376
17	18.62380974008323	27.792581766712786	30.834197056269545	22.74941143693444
18	18.462374330854807	23.92626641892675	30.531187911087493	27.080171339130953
19	19.658209751471492	25.850815108158	28.859926396570508	25.631048743800005
20	26.07681790234971	25.12396598976548	30.927641822854213	17.871574285030604
21	23.16088023139866	30.809827528821458	27.376672905356074	18.652619334423804
22	21.077370368688243	22.379462351961052	32.6742488999819	23.868918379368807
23	23.0575046281766	27.173006020188406	28.671003244977133	21.098486106657862
24	21.0052447019156	26.471204302051987	28.211032651099544	24.31251834493287
25	19.341473681927166	32.64584602932613	25.354985042736487	22.65769524601021
26	20.35885908583429	25.208361154363164	30.960959271368075	23.47182048843447
27	25.20178578812543	26.22259444971302	26.5269593405111	22.048660421650442
28	18.224508762522852	28.974147964720785	31.208281165371815	21.59306210738454
29	23.07519710846575	22.429591046113654	30.595958657893185	23.89925318752741
30	21.563574640235956	28.74733172315946	28.764244649719394	20.924848986885195
31	22.083299722139934	24.934026028960083	26.377895110028927	26.60477913887106
32	25.645148498206687	27.717981864190893	24.440636305647427	22.196233331954993
33	23.435622080486553	24.08895589285	25.64728379755193	26.82813822911152
34	22.905152714575554	25.682058672603027	26.823325332174626	24.589463280646797
35	24.378915986477793	24.912774716428856	26.544177309834648	24.164131987258703
36	22.362210488997107	27.37555441522285	28.683781147408187	21.578453948371852
37	21.87373534354255	25.515878153718507	28.60484285891501	24.005543643823934
38	19.147873207958497	24.768930106568384	28.221573573264152	27.861623112208967
39	20.92922126649688	27.360641213446556	26.606033203565882	25.104104316490677
40	19.68400281181641	25.39192911077323	28.09820072220569	26.825867355204675
41	18.5972032323687	28.11836743824409	28.35087781139274	24.933551517994474
42	21.3155070861434	26.364913845755467	29.405715687942607	22.913863380158528
43	22.22416169164515	24.428841318787992	28.893820036971185	24.453176952595676
44	22.684301753724743	23.374240697720925	29.619042260624468	24.322415287929864
45	21.193557767981762	22.239820553510263	28.62023057165692	27.94639110685106
46	24.491239510765634	26.176194056004498	26.200190753408176	23.132375679821692
47	18.389841940397357	25.92955003480877	32.43682394897516	23.24378407581872
48	20.036632246545054	26.101051855236197	28.75597460146163	25.106341296757122
49	20.342556244801564	23.067774401218003	32.82697364362735	23.762695710353082
50	20.98368834662077	23.02520398887475	29.740889897864903	26.250217766639572
51	19.30974923451213	22.64468008809635	28.871382447025933	29.174188230365584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	411.0
1	1233.5
2	2056.0
3	4650.0
4	7244.0
5	5382.5
6	3521.0
7	3378.5
8	3236.0
9	3346.0
10	3456.0
11	3392.5
12	3329.0
13	3635.5
14	3942.0
15	3873.0
16	3804.0
17	4353.0
18	4902.0
19	6010.5
20	7119.0
21	8576.0
22	10033.0
23	13671.5
24	17310.0
25	19561.5
26	27608.5
27	33404.0
28	39845.5
29	46287.0
30	54272.0
31	62257.0
32	71193.5
33	80130.0
34	86361.0
35	92592.0
36	97725.5
37	102859.0
38	112128.5
39	121398.0
40	132632.5
41	143867.0
42	153601.0
43	163335.0
44	182873.0
45	202411.0
46	300862.5
47	399314.0
48	342422.0
49	285530.0
50	269733.5
51	253937.0
52	222547.5
53	191158.0
54	172387.0
55	153616.0
56	145055.5
57	136495.0
58	125694.0
59	114893.0
60	104110.0
61	93327.0
62	78982.0
63	64637.0
64	55199.5
65	45762.0
66	36966.5
67	28171.0
68	22185.0
69	16199.0
70	13401.0
71	10603.0
72	8818.5
73	7034.0
74	5632.0
75	3415.5
76	2601.0
77	1950.5
78	1300.0
79	969.0
80	638.0
81	426.0
82	214.0
83	121.0
84	28.0
85	15.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2950406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.57586755480649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69401163898186	15.959786436796028
2	12.26309810982498	5.537001574780131
3	4.804182646221328	3.2537577339057737
4	2.35354852920104	2.1253359951620916
5	1.3386858124711638	1.5110996799923755
6	0.8718362537440757	1.180947587640295
7	0.5897449402109466	0.9319802562923721
8	0.4328002809446358	0.7816673456231309
9	0.34829591135181537	0.7076774128103286
>10	4.414437686801612	25.752467316190447
>50	1.7312785010851526	26.27211759077853
>100	0.14707776896547398	5.678003662419417
>500	0.005177374209877914	0.8199392568237808
>1k	0.00372123771334975	1.6782675086102217
>5k	0.0014561364965281632	2.6164419478261345
>10k+	6.471717762347392E-4	5.1935086943489885
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	46933	1.5907302249249764	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	42231	1.4313623277609928	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	37277	1.2634532332160389	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCCAATT	15745	0.5336553681086603	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	9724	0.3295817592561837	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTC	9458	0.3205660509096036	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCT	9148	0.31005902238539373	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	8923	0.3024329532952414	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTA	8636	0.2927054785002471	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	7737	0.2622350957800384	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	7329	0.2484064904965622	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	5610	0.1901433226477983	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	5067	0.17173907591023066	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	4350	0.1474373357429452	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGT	3149	0.10673107362173205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.10029128194560341	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0504276360609356E-4	0.0	0.0	0.27575865829990853	0.0
2	3.0504276360609356E-4	0.0	0.0	1.0991029709131557	0.0
3	3.0504276360609356E-4	0.0	0.0	1.482135001081207	0.0
4	3.0504276360609356E-4	0.0	0.0	2.212407377154195	0.0
5	3.0504276360609356E-4	0.0	0.0	4.227418192614847	0.0
6	3.0504276360609356E-4	0.0	0.0	4.831063928150905	0.0
7	3.0504276360609356E-4	0.0	0.0	5.6568485828730015	0.0
8	3.0504276360609356E-4	0.0	0.0	6.750901401366456	0.0
9	3.0504276360609356E-4	0.0	0.0	7.1090893931208115	0.0
10	3.0504276360609356E-4	0.0	0.0	9.445988111466693	0.0
11	3.0504276360609356E-4	0.0	0.0	10.653516838021615	0.0
12	3.0504276360609356E-4	0.0	0.0	13.66042503980808	0.0
13	3.0504276360609356E-4	0.0	0.0	14.122327571188508	0.0
14	3.0504276360609356E-4	0.0	0.0	14.35568528534717	0.0
15	3.0504276360609356E-4	0.0	0.0	14.91235443528789	0.0
16	3.389364040067706E-4	0.0	0.0	15.479937337437628	0.0
17	3.389364040067706E-4	0.0	0.0	16.146693031399746	0.0
18	3.389364040067706E-4	0.0	0.0	16.799213396393583	0.0
19	3.389364040067706E-4	0.0	0.0	17.992947411305426	0.0
20	3.7283004440744766E-4	0.0	0.0	18.581951094188394	0.0
21	4.067236848081247E-4	0.0	0.0	19.267992269538496	0.0
22	4.067236848081247E-4	0.0	0.0	20.066662011940053	0.0
23	4.067236848081247E-4	0.0	0.0	20.75378778378298	0.0
24	4.4061732520880176E-4	0.0	0.0	21.285917938073606	0.0
25	7.795537292155724E-4	0.0	0.0	21.755378751263386	0.0
26	7.795537292155724E-4	0.0	0.0	22.19250503151092	0.0
27	7.795537292155724E-4	0.0	0.0	22.660406737242266	0.0
28	7.795537292155724E-4	0.0	0.0	23.106989343161587	0.0
29	8.134473696162494E-4	0.0	0.0	23.578856604819812	0.0
30	8.134473696162494E-4	0.0	0.0	24.10993605625802	0.0
31	8.134473696162494E-4	0.0	0.0	24.605833908960324	0.0
32	8.134473696162494E-4	0.0	0.0	25.06112718046262	0.0
33	8.134473696162494E-4	0.0	0.0	25.502862995804644	0.0
34	8.134473696162494E-4	0.0	0.0	25.944869960269873	0.0
35	8.473410100169265E-4	0.0	0.0	26.41561873179488	0.0
36	8.473410100169265E-4	0.0	0.0	26.87935151975694	0.0
37	8.473410100169265E-4	0.0	0.0	27.344948457941044	0.0
38	8.473410100169265E-4	0.0	0.0	27.808003373095094	0.0
39	8.473410100169265E-4	0.0	0.0	28.28346336063579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCG	20	7.035087E-4	45.0	45
CGATCGA	380	0.0	43.81579	41
TAATACG	1800	0.0	43.5	4
AATACGG	1845	0.0	42.439026	5
CGTATGG	350	0.0	42.428574	2
TACGGGA	1470	0.0	42.39796	4
CCGATCG	395	0.0	42.151897	40
TACGGCT	6105	0.0	42.08845	7
CGCATGG	290	0.0	41.896553	2
ACGGGAT	995	0.0	41.83417	5
CGATGAA	9795	0.0	41.761105	19
TATGGGA	4790	0.0	41.7119	4
CGCGATC	65	0.0	41.53846	43
TGAATGA	10750	0.0	41.086044	22
CCGATGA	9730	0.0	41.06886	18
ATGAATG	10840	0.0	41.056274	21
ACGGCTG	6280	0.0	40.915604	8
GATGAAT	10995	0.0	40.804913	20
TTATGGG	5905	0.0	40.73243	3
CTACGGG	790	0.0	40.158226	3
>>END_MODULE