##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935337.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506841
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34499592193204	33.0	31.0	34.0	30.0	34.0
2	32.495294460397616	34.0	31.0	34.0	31.0	34.0
3	32.546583879785594	34.0	31.0	34.0	31.0	34.0
4	35.97559463805405	37.0	35.0	37.0	35.0	37.0
5	31.544371303939833	37.0	35.0	37.0	0.0	37.0
6	33.369591084925354	37.0	35.0	37.0	17.0	37.0
7	35.206258656354585	37.0	35.0	37.0	32.0	37.0
8	35.76916476257283	37.0	35.0	37.0	35.0	37.0
9	37.796919515728604	39.0	38.0	39.0	35.0	39.0
10	37.56229290283447	39.0	37.0	39.0	35.0	39.0
11	37.4796000374293	39.0	37.0	39.0	35.0	39.0
12	37.270652975330506	39.0	37.0	39.0	34.0	39.0
13	37.202645136414525	39.0	37.0	39.0	34.0	39.0
14	38.38409361040747	40.0	38.0	41.0	34.0	41.0
15	38.4673193787533	40.0	38.0	41.0	34.0	41.0
16	38.53577782924675	40.0	38.0	41.0	34.0	41.0
17	38.53100161198162	40.0	38.0	41.0	34.0	41.0
18	38.524712959097876	40.0	38.0	41.0	34.0	41.0
19	38.52852225284552	40.0	38.0	41.0	34.0	41.0
20	38.464016442345276	40.0	38.0	41.0	34.0	41.0
21	38.39250458409348	40.0	37.0	41.0	34.0	41.0
22	38.357015106437906	40.0	37.0	41.0	34.0	41.0
23	38.33005008491274	40.0	37.0	41.0	34.0	41.0
24	38.315977598167294	40.0	37.0	41.0	34.0	41.0
25	38.21872247967768	40.0	37.0	41.0	34.0	41.0
26	38.15501768268849	40.0	37.0	41.0	34.0	41.0
27	38.100893856750645	40.0	37.0	41.0	34.0	41.0
28	38.07394808078623	40.0	37.0	41.0	34.0	41.0
29	38.012836789017555	40.0	37.0	41.0	34.0	41.0
30	37.92769841011759	40.0	36.0	41.0	34.0	41.0
31	37.750867543423624	40.0	36.0	41.0	33.0	41.0
32	37.80625759453054	40.0	36.0	41.0	33.0	41.0
33	37.77840661357104	40.0	36.0	41.0	33.0	41.0
34	37.76736297990299	40.0	36.0	41.0	33.0	41.0
35	37.69027787271517	40.0	36.0	41.0	33.0	41.0
36	37.66097152917926	40.0	36.0	41.0	33.0	41.0
37	37.65098706499226	40.0	36.0	41.0	33.0	41.0
38	37.6610319204216	40.0	36.0	41.0	33.0	41.0
39	37.61606831775881	40.0	36.0	41.0	33.0	41.0
40	37.519551166977806	40.0	35.0	41.0	33.0	41.0
41	37.484601228663145	40.0	35.0	41.0	33.0	41.0
42	37.48768649114273	40.0	35.0	41.0	33.0	41.0
43	37.449356634177065	39.0	35.0	41.0	33.0	41.0
44	37.393109823796934	39.0	35.0	41.0	33.0	41.0
45	37.35687176019235	39.0	35.0	41.0	33.0	41.0
46	37.24515658918227	39.0	35.0	41.0	33.0	41.0
47	37.22133722137903	39.0	35.0	41.0	33.0	41.0
48	37.202416844245676	39.0	35.0	41.0	33.0	41.0
49	37.17801745505996	39.0	35.0	41.0	33.0	41.0
50	37.095183234329305	39.0	35.0	41.0	33.0	41.0
51	36.45022533897073	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	10.0
11	12.0
12	17.0
13	18.0
14	27.0
15	34.0
16	54.0
17	109.0
18	213.0
19	325.0
20	519.0
21	834.0
22	1433.0
23	2131.0
24	3197.0
25	4684.0
26	6485.0
27	8363.0
28	10640.0
29	13586.0
30	17662.0
31	24173.0
32	33306.0
33	51051.0
34	86763.0
35	123790.0
36	121892.0
37	195596.0
38	319372.0
39	479969.0
40	568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.37669269684061	4.6219209591456565	54.61744138897203	16.383944955041706
2	20.074911686103576	4.609643618669787	52.91069197081843	22.404752724408215
3	21.756110963266863	4.674813069195754	50.34837783150312	23.220698136034258
4	19.205211432393995	5.356305011610382	49.66403223697789	25.774451319017732
5	16.025911161164316	17.78634905739889	43.32261997118475	22.865119810252043
6	21.429201886595862	6.5985727757606805	51.961819462040125	20.010405875603333
7	82.9260685102144	2.92632069342419	9.113171197226515	5.0344395991348785
8	84.03308643712244	3.1538828582444993	7.191601502746474	5.6214292018865955
9	78.17068954189592	6.230053469476872	9.577121939209245	6.022135049417955
10	36.81908044710756	27.979528032486506	18.16223476796822	17.039156752437716
11	28.592134140231117	25.742596597783045	25.28853409218358	20.376735169802256
12	26.09392762740064	22.429572861370247	29.515390144016525	21.9611093672126
13	23.475668633916914	25.160717023229388	30.550867676151633	20.81274666670206
14	19.99043031082908	26.77455683778182	30.992387385264937	22.242625466124164
15	18.346328511103692	24.951272231111314	34.44663371915152	22.255765538633472
16	20.53421694790625	25.032369042254626	31.843505718254285	22.589908291584845
17	20.680416845572957	24.135924095508418	29.195183831605327	25.9884752273133
18	21.82572680196517	23.50626243910273	30.973407280529266	23.694603478402833
19	22.896244527458435	25.464597791007808	29.473779914403707	22.165377767130042
20	25.61152769270281	26.346575385193262	28.008197281597724	20.033699640506196
21	24.35067800783228	26.708391927217274	28.116237877785384	20.82469218716507
22	22.374557103237834	25.055729171160063	28.718424837126143	23.851288888475956
23	21.351954187601745	25.899348371858743	27.718651138374916	25.030046302164592
24	22.65879412625486	24.99036062862638	29.23460404913325	23.11624119598551
25	21.837340502415316	25.899348371858743	27.0321155317648	25.231195593961143
26	19.807597483742477	28.25454045914599	26.732747516161293	25.20511454095024
27	20.783612869572835	27.215147450859117	28.154861727282444	23.84637795228561
28	19.04215507807393	26.139984245185786	29.913507795447565	24.90435288129272
29	19.967667457946792	24.75470205549225	29.38916581112407	25.88846467543689
30	20.77472009322815	26.12830418073307	28.99137997970589	24.10559574633289
31	22.761591966239305	27.7373657871003	25.08074840012981	24.420293846530587
32	22.177787835611056	27.10524866259944	25.815796092620257	24.901167409169247
33	21.948168386711007	26.132352384889977	27.32697079519339	24.592508433205627
34	20.215271551543925	25.509791676759523	28.03248650653918	26.242450265157373
35	20.16012306540637	24.97224325592415	27.487837137428567	27.379796541240914
36	20.914084498629915	26.999796262512106	27.983576236643415	24.102543002214567
37	20.60270459856083	27.75501861178452	27.971896172190696	23.67038061746395
38	21.335628642968967	26.779335045967027	26.597165858906145	25.287870452157858
39	21.658423151480484	24.63883050700107	29.75303963722782	23.949706704290634
40	21.376774324563772	25.024737181958812	29.195582015620758	24.402906477856657
41	18.76130261918809	25.819047928746297	27.51358637042661	27.906063081639005
42	20.099997279075897	24.86785267987797	28.015165501867816	27.016984539178317
43	21.34445505531108	24.234275547320518	28.81511718887394	25.60615220849446
44	21.100036433837413	24.232284627243352	27.916017682024847	26.751661256894387
45	21.441147407058875	24.601202117542595	26.336089872786843	27.621560602611687
46	20.713200662843658	25.296630500497397	28.446863338600426	25.543305498058523
47	18.750087102753376	26.199313663485395	29.550430337374678	25.500168896386548
48	19.20886145253547	24.489976049231473	30.051677648803025	26.249484849430033
49	20.99378766571921	22.878060790753636	30.96823088832863	25.159920655198526
50	20.237569856408207	23.445406648743962	29.142225357552654	27.174798137295177
51	18.81446018524848	23.711327207051042	28.02034189406845	29.45387071363203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	351.0
1	762.5
2	1174.0
3	1878.0
4	2582.0
5	1923.0
6	1264.0
7	1450.5
8	1637.0
9	1913.5
10	2190.0
11	2157.0
12	2124.0
13	2199.0
14	2274.0
15	2511.5
16	2749.0
17	2640.5
18	2532.0
19	2701.5
20	2871.0
21	4132.0
22	5393.0
23	6188.5
24	6984.0
25	8162.5
26	10938.5
27	12536.0
28	15598.0
29	18660.0
30	21170.0
31	23680.0
32	26767.0
33	29854.0
34	34833.0
35	39812.0
36	42873.0
37	45934.0
38	51543.5
39	57153.0
40	64039.0
41	70925.0
42	80948.0
43	90971.0
44	101665.0
45	112359.0
46	126121.5
47	139884.0
48	155739.0
49	171594.0
50	165406.0
51	159218.0
52	136255.0
53	113292.0
54	100063.5
55	86835.0
56	79208.5
57	71582.0
58	64585.5
59	57589.0
60	52410.5
61	47232.0
62	41431.0
63	35630.0
64	31719.5
65	27809.0
66	23749.5
67	19690.0
68	15569.0
69	11448.0
70	10430.5
71	9413.0
72	7251.0
73	5089.0
74	3858.5
75	1833.5
76	1039.0
77	924.0
78	809.0
79	689.0
80	569.0
81	321.0
82	73.0
83	42.0
84	11.0
85	14.5
86	18.0
87	26.5
88	35.0
89	19.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1506841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.03246507633756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.73526258515977	16.278453325144064
2	12.374732150195912	5.9479063645722965
3	5.474642687632244	3.9470747758784603
4	2.928583683396969	2.8152434037747867
5	1.9304379085224115	2.3196590809301485
6	1.2540824533321184	1.8083215657531115
7	0.9302106535708177	1.5648678531904467
8	0.7238580932351004	1.3916875476717476
9	0.5832163252293716	1.261451337122232
>10	4.177331700401575	21.172232409203048
>50	1.5281365835890623	26.523544172118086
>100	0.3492809701696706	11.652368102907012
>500	0.006355587243111012	0.9992544465360145
>1k	0.0033159585616231364	1.568245746989299
>5k	5.526597602705227E-4	0.7496898682092821
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	5786	0.38398211888314693	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	5503	0.36520110615519485	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	4978	0.3303600048047538	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	4811	0.3192772163751849	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2153	0.1428816975380946	No Hit
TCCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTG	2073	0.1375725773323131	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3272800514453747E-4	0.0	0.0	0.3967240073770225	0.0
2	1.3272800514453747E-4	0.0	0.0	1.4813109014156105	0.0
3	1.3272800514453747E-4	0.0	0.0	2.079516020602041	0.0
4	1.3272800514453747E-4	0.0	0.0	2.789942668138178	0.0
5	1.3272800514453747E-4	0.0	0.0	3.965448245700774	0.0
6	1.3272800514453747E-4	0.0	0.0	4.768253584817509	0.0
7	1.3272800514453747E-4	0.0	0.0	5.507216753459721	0.0
8	1.3272800514453747E-4	0.0	0.0	6.647881229671876	0.0
9	1.3272800514453747E-4	0.0	0.0	7.216222547700786	0.0
10	1.3272800514453747E-4	0.0	0.0	8.228472678935601	0.0
11	1.3272800514453747E-4	0.0	0.0	10.099473003455573	0.0
12	1.3272800514453747E-4	0.0	0.0	11.681524460775888	0.0
13	1.3272800514453747E-4	0.0	0.0	12.184629964276258	0.0
14	1.3272800514453747E-4	0.0	0.0	12.37018371546832	0.0
15	1.3272800514453747E-4	0.0	0.0	12.664043518858326	0.0
16	1.3272800514453747E-4	0.0	0.0	13.401679407449095	0.0
17	1.3272800514453747E-4	0.0	0.0	14.42507869111605	0.0
18	1.9909200771680622E-4	0.0	0.0	15.520615645579062	0.0
19	1.9909200771680622E-4	0.0	0.0	16.29017261940709	0.0
20	1.9909200771680622E-4	0.0	0.0	17.04519587667179	0.0
21	1.9909200771680622E-4	0.0	0.0	18.092818021277626	0.0
22	1.9909200771680622E-4	0.0	0.0	19.102280864404406	0.0
23	1.9909200771680622E-4	0.0	0.0	20.05480339332418	0.0
24	3.318200128613437E-4	0.0	0.0	20.814339402763796	0.0
25	3.318200128613437E-4	0.0	0.0	21.457273859683934	0.0
26	3.318200128613437E-4	0.0	0.0	22.09051917222852	0.0
27	3.318200128613437E-4	0.0	0.0	22.67863696302397	0.0
28	3.318200128613437E-4	0.0	0.0	23.305776787331908	0.0
29	3.9818401543361244E-4	0.0	0.0	23.988994193813415	0.0
30	3.9818401543361244E-4	0.0	0.0	24.764192107860087	0.0
31	3.9818401543361244E-4	0.0	0.0	25.420863913312687	0.0
32	3.9818401543361244E-4	0.0	0.0	26.033602749062442	0.0
33	3.9818401543361244E-4	0.0	0.0	26.621919631865605	0.0
34	3.9818401543361244E-4	0.0	0.0	27.23134026748675	0.0
35	3.9818401543361244E-4	0.0	0.0	27.8685010561831	0.0
36	3.9818401543361244E-4	0.0	0.0	28.492057224352138	0.0
37	3.9818401543361244E-4	0.0	0.0	29.0870768714151	0.0
38	3.9818401543361244E-4	0.0	0.0	29.70831029949411	0.0
39	4.645480180058812E-4	0.0	0.0	30.391660433980757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	30	2.1659525E-6	45.000004	8
ATCGTTA	30	2.1659525E-6	45.000004	38
CGTCGCA	30	2.1659525E-6	45.000004	14
GTCGATG	40	6.8175723E-9	45.0	1
ACGTTTA	25	3.891386E-5	45.0	17
TTAGCCG	25	3.891386E-5	45.0	15
AACCCGC	25	3.891386E-5	45.0	24
ATTACGC	20	7.033987E-4	45.0	41
TACGGGT	170	0.0	45.0	4
CGCGAAA	25	3.891386E-5	45.0	23
AGACGGG	20	7.033987E-4	45.0	3
CGTAGTC	25	3.891386E-5	45.0	31
GTATGCG	20	7.033987E-4	45.0	1
TGTTACG	220	0.0	45.0	1
TTACGTA	20	7.033987E-4	45.0	11
ACCCGTA	20	7.033987E-4	45.0	17
CAATCGA	25	3.891386E-5	45.0	40
TAGTCGT	20	7.033987E-4	45.0	37
GCGTACC	20	7.033987E-4	45.0	43
ACGACTA	20	7.033987E-4	45.0	10
>>END_MODULE