SRR2935335.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1881550
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	4464	0.2372512024660519	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	4379	0.2327336504477691	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	4178	0.2220509686163004	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3926	0.20865775557386196	TruSeq Adapter, Index 21 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	25	3.891757E-5	45.0	15
CGTAAGT	25	3.891757E-5	45.0	12
CGTAAGC	20	7.034434E-4	45.0	33
CTCGACG	20	7.034434E-4	45.0	1
TCGAGTA	20	7.034434E-4	45.0	31
TGCGTCA	20	7.034434E-4	45.0	19
CTTCGAC	30	2.1662418E-6	44.999996	33
TCTACGG	340	0.0	41.691177	2
TTCACGG	370	0.0	41.351353	2
TAAGCGA	60	3.6379788E-12	41.249996	27
TTTGACG	165	0.0	40.90909	1
TACGGGA	540	0.0	40.416668	4
CTACGGG	535	0.0	40.373833	3
TACGGGC	375	0.0	40.2	4
TTATGGG	3925	0.0	39.78344	3
TTACGGG	1115	0.0	39.75336	3
CGTATGG	250	0.0	39.600002	2
CCCGTTA	40	3.4597906E-7	39.375	32
TACGCGC	40	3.4597906E-7	39.375	34
TGGGCGA	3245	0.0	38.967644	6