##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757943
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.245007869984946	33.0	31.0	34.0	30.0	34.0
2	32.374476708670706	34.0	31.0	34.0	30.0	34.0
3	32.44421282339173	34.0	31.0	34.0	30.0	34.0
4	35.92497193060692	37.0	35.0	37.0	35.0	37.0
5	31.59353143969929	37.0	35.0	37.0	0.0	37.0
6	33.29433479826319	37.0	35.0	37.0	17.0	37.0
7	35.06125262717645	37.0	35.0	37.0	32.0	37.0
8	35.6294022109842	37.0	35.0	37.0	35.0	37.0
9	37.64875722844594	39.0	37.0	39.0	35.0	39.0
10	37.412214374959596	39.0	37.0	39.0	35.0	39.0
11	37.38540101300494	39.0	37.0	39.0	35.0	39.0
12	37.12232582133485	39.0	37.0	39.0	34.0	39.0
13	36.984994913865556	39.0	37.0	39.0	33.0	39.0
14	38.13653137505063	40.0	37.0	41.0	33.0	41.0
15	38.26013037919738	40.0	37.0	41.0	34.0	41.0
16	38.35482087703165	40.0	37.0	41.0	34.0	41.0
17	38.339293324168175	40.0	37.0	41.0	34.0	41.0
18	38.33173998572452	40.0	37.0	41.0	34.0	41.0
19	38.35913782434827	40.0	37.0	41.0	34.0	41.0
20	38.2441450082658	40.0	37.0	41.0	34.0	41.0
21	38.182532195692815	40.0	37.0	41.0	34.0	41.0
22	38.133157770439205	40.0	37.0	41.0	34.0	41.0
23	38.107626826819434	40.0	37.0	41.0	34.0	41.0
24	38.112292085288736	40.0	37.0	41.0	34.0	41.0
25	37.9747329284656	40.0	36.0	41.0	34.0	41.0
26	37.93915505519544	40.0	36.0	41.0	34.0	41.0
27	37.82765194744196	40.0	36.0	41.0	33.0	41.0
28	37.721176922275156	40.0	36.0	41.0	33.0	41.0
29	37.61911647709656	40.0	36.0	41.0	33.0	41.0
30	37.579662322892354	40.0	36.0	41.0	33.0	41.0
31	37.44379194741557	40.0	35.0	41.0	33.0	41.0
32	37.52727447842384	40.0	35.0	41.0	33.0	41.0
33	37.49784482474276	40.0	35.0	41.0	33.0	41.0
34	37.47835127443621	40.0	35.0	41.0	33.0	41.0
35	37.3969612490649	40.0	35.0	41.0	33.0	41.0
36	37.36265655860665	39.0	35.0	41.0	33.0	41.0
37	37.30678428325085	39.0	35.0	41.0	33.0	41.0
38	37.29827704721859	39.0	35.0	41.0	33.0	41.0
39	37.30601773484286	39.0	35.0	41.0	33.0	41.0
40	37.216133667043565	39.0	35.0	41.0	32.0	41.0
41	37.10872717341542	39.0	35.0	41.0	32.0	41.0
42	37.09296609375639	39.0	35.0	41.0	32.0	41.0
43	36.98427586243292	39.0	35.0	41.0	32.0	41.0
44	36.92794708836944	39.0	35.0	41.0	32.0	41.0
45	36.90730569449154	39.0	35.0	41.0	32.0	41.0
46	36.80925742437096	39.0	35.0	41.0	31.0	41.0
47	36.77538680349314	39.0	35.0	41.0	31.0	41.0
48	36.73838270160157	39.0	35.0	41.0	31.0	41.0
49	36.66710293518114	39.0	35.0	40.0	31.0	41.0
50	36.49390785322907	38.0	35.0	40.0	31.0	41.0
51	35.83632806160885	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	34.0
11	41.0
12	29.0
13	25.0
14	27.0
15	24.0
16	52.0
17	70.0
18	122.0
19	246.0
20	433.0
21	734.0
22	1042.0
23	1568.0
24	2143.0
25	2945.0
26	4048.0
27	5077.0
28	6390.0
29	8047.0
30	10486.0
31	13950.0
32	18997.0
33	28640.0
34	47062.0
35	72553.0
36	61932.0
37	96357.0
38	155063.0
39	219561.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.754820613159563	4.36721494888138	56.152111702331176	15.725852735627877
2	17.542084299215112	6.819378238205248	54.89225443074215	20.746283031837486
3	19.096290882032026	6.585719506611974	53.06454443144142	21.253445179914586
4	17.746585165375233	3.814270993992952	53.958938864795904	24.480204975835914
5	17.559235984764026	16.714977247629438	44.172187090585965	21.553599677020568
6	19.185083838758324	7.9703882745800145	52.77600030609162	20.06852758057004
7	80.86307281682132	2.4158808775857814	11.545723095272335	5.175323210320565
8	83.11891527463146	4.153874367861436	7.095916183670805	5.631294173836291
9	77.48894046122203	5.685915695507446	9.176679512839355	7.64846433043118
10	38.56767593341452	28.729073294429792	16.47247880117634	16.230771970979347
11	30.243039384228105	27.669099127506954	23.54477843320672	18.54308305505823
12	28.29896179528012	20.697862504172477	30.408223309668404	20.594952390878998
13	24.054579302137498	25.585960949570087	30.46020611048588	19.899253637806538
14	18.387926268861907	30.730015317774555	28.86496741839426	22.01709099496928
15	15.069207051189865	24.72639763148416	39.52408030683046	20.680315010495512
16	17.067378417638267	24.453817767299125	34.36722814248565	24.11157567257696
17	18.96145224640903	22.50459467268647	28.548848660123515	29.985104420780985
18	21.08878899864502	23.425904058748483	30.307292237015183	25.178014705591316
19	23.825274459952794	24.059856743844854	28.672340796075694	23.442528000126657
20	26.32321955608799	25.238177541055194	27.42119130330381	21.017411599553
21	24.515563835275213	26.453704302302416	27.718707079556115	21.312024782866256
22	21.5179769454959	25.06613294139533	28.14829083453505	25.267599278573723
23	20.155473432698763	26.861518610238498	26.748317485615676	26.23469047144706
24	22.123959189543278	23.851265860361533	29.087147714274032	24.937627235821164
25	20.80011293725254	26.064888784512817	26.4110889605155	26.72390931771914
26	19.821147500537638	27.64469095961042	26.73815841032901	25.796003129522934
27	21.11227361424276	28.745829171850655	26.39367340288122	23.74822381102537
28	19.897802341337016	26.310157887862278	30.493717865327604	23.298321905473102
29	20.406415785883635	24.83352969814353	29.543382549875126	25.216671966097714
30	21.692000585796027	28.042346192259842	26.31609500978306	23.949558212161072
31	21.647406203368856	28.11095293445549	25.096742103298002	25.14489875887765
32	23.298321905473102	27.766072118879652	26.116871585330298	22.81873439031695
33	22.40247617564909	27.33846740454097	25.55667114809425	24.70238527171568
34	21.387096391153424	25.072729743529525	28.619566379002116	24.920607486314932
35	20.794967431587864	25.909204254145763	27.359445235327723	25.93638307893865
36	24.241928482748705	26.670343284389457	26.120565794525447	22.96716243833639
37	20.405624169627533	28.065830807857584	28.197502978456164	23.331042044058723
38	19.92709214281285	26.999259838800544	25.284355155994582	27.789292862392028
39	22.613441907900725	25.065473261181907	27.68717436535465	24.633910465562714
40	23.931351038270687	23.229583227234766	29.34574235793457	23.493323376559978
41	20.01469767515499	25.071674255188054	28.97552982216341	25.938098247493546
42	23.520238329267503	23.64610531398799	28.386039583451527	24.447616773292978
43	24.120415387436786	24.255517895145147	26.097345051013072	25.526721666404995
44	20.8776913303507	24.320958172316388	27.599832705097878	27.201517792235038
45	20.0664166038871	24.74882675874043	25.57474638594195	29.61001025143052
46	22.471082917844747	25.620924000881335	27.596666240073464	24.311326841200458
47	19.25078798801493	24.70238527171568	31.321088788998647	24.725737951270744
48	20.072617597893245	23.656924069488074	29.169475804908814	27.100982527709867
49	21.75915603152216	21.837657976919107	31.308159056815615	25.095026934743114
50	20.635720628068338	23.12812441041081	30.050148889824168	26.18600607169668
51	20.122093613899725	23.277212138643673	26.570995444248446	30.029698803208156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	260.5
2	421.0
3	739.0
4	1057.0
5	789.5
6	522.0
7	629.0
8	736.0
9	921.0
10	1106.0
11	1122.5
12	1139.0
13	1408.0
14	1677.0
15	1480.0
16	1283.0
17	1394.0
18	1505.0
19	1299.0
20	1093.0
21	1382.0
22	1671.0
23	2118.5
24	2566.0
25	3802.5
26	5969.0
27	6899.0
28	7623.0
29	8347.0
30	9197.0
31	10047.0
32	11428.5
33	12810.0
34	14785.0
35	16760.0
36	17681.5
37	18603.0
38	21309.5
39	24016.0
40	30156.0
41	36296.0
42	42985.5
43	49675.0
44	57476.0
45	65277.0
46	76208.5
47	87140.0
48	89477.0
49	91814.0
50	85241.0
51	78668.0
52	66255.0
53	53842.0
54	46783.0
55	39724.0
56	36878.0
57	34032.0
58	33355.0
59	32678.0
60	28944.5
61	25211.0
62	21180.5
63	17150.0
64	14322.5
65	11495.0
66	8852.5
67	6210.0
68	5362.0
69	4514.0
70	3523.5
71	2533.0
72	2241.0
73	1949.0
74	1599.5
75	859.0
76	468.0
77	355.0
78	242.0
79	245.0
80	248.0
81	186.0
82	124.0
83	63.5
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.404773876852015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.8123095371143	18.169734732714502
2	12.317411024893374	6.504769057211073
3	5.347170111073771	4.235724529918937
4	2.942972856613135	3.108341312183322
5	1.9405217808981836	2.5619519413861354
6	1.2281761525471584	1.9457828153369896
7	0.9567268009262735	1.7683508388267586
8	0.7056955490936349	1.4906985119774678
9	0.5576978695709882	1.3253297523861647
>10	3.6608728764593494	20.030865532903263
>50	1.199132529817838	22.73115843559412
>100	0.31980203946208696	12.088564636234674
>500	0.007507090128218003	1.256998227093851
>1k	0.004003781401716268	2.781729676232713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	3589	0.4735184571926913	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	3217	0.42443824931426244	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	3210	0.4235146970154748	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	2964	0.39105843051522343	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	2351	0.310181636349963	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	2225	0.29355769497178547	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	2151	0.28379442781317327	No Hit
TCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1343	0.17719010532454288	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	883	0.11649952568992655	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	804	0.10607657831789462	No Hit
GCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	773	0.10198656099469222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.405043651039722	0.0
2	1.3193604268394853E-4	0.0	0.0	1.8178147960994429	0.0
3	1.3193604268394853E-4	0.0	0.0	2.5412201181355325	0.0
4	1.3193604268394853E-4	0.0	0.0	3.3952421224287312	0.0
5	2.6387208536789706E-4	0.0	0.0	5.036658429459735	0.0
6	2.6387208536789706E-4	0.0	0.0	6.039108481772376	0.0
7	2.6387208536789706E-4	0.0	0.0	6.935481955767123	0.0
8	2.6387208536789706E-4	0.0	0.0	8.308012607808239	0.0
9	2.6387208536789706E-4	0.0	0.0	8.997642302917237	0.0
10	2.6387208536789706E-4	0.0	0.0	10.523614572599786	0.0
11	2.6387208536789706E-4	0.0	0.0	12.486954823779625	0.0
12	2.6387208536789706E-4	0.0	0.0	14.599118931106956	0.0
13	3.958081280518456E-4	0.0	0.0	15.38479806528987	0.0
14	3.958081280518456E-4	0.0	0.0	15.71490204408511	0.0
15	3.958081280518456E-4	0.0	0.0	16.1169111661431	0.0
16	3.958081280518456E-4	0.0	0.0	16.93517850286895	0.0
17	3.958081280518456E-4	0.0	0.0	18.126956776433055	0.0
18	5.277441707357941E-4	0.0	0.0	19.57350354841987	0.0
19	5.277441707357941E-4	0.0	0.0	20.497319719292875	0.0
20	5.277441707357941E-4	0.0	0.0	21.37126406603135	0.0
21	5.277441707357941E-4	0.0	0.0	22.534939962503778	0.0
22	5.277441707357941E-4	0.0	0.0	23.649667587140456	0.0
23	5.277441707357941E-4	0.0	0.0	24.67151223772764	0.0
24	6.596802134197427E-4	0.0	0.0	25.433574820270128	0.0
25	7.916162561036912E-4	0.0	0.0	26.125315492062068	0.0
26	7.916162561036912E-4	0.0	0.0	26.77127435704268	0.0
27	7.916162561036912E-4	0.0	0.0	27.412087716358617	0.0
28	7.916162561036912E-4	0.0	0.0	28.01727834414989	0.0
29	7.916162561036912E-4	0.0	0.0	28.685402464301404	0.0
30	7.916162561036912E-4	0.0	0.0	29.40867585029481	0.0
31	9.235522987876397E-4	0.0	0.0	30.14408735221514	0.0
32	9.235522987876397E-4	0.0	0.0	30.79466397868969	0.0
33	0.0010554883414715882	0.0	0.0	31.445108669121556	0.0
34	0.0010554883414715882	0.0	0.0	32.10689985922424	0.0
35	0.0010554883414715882	0.0	0.0	32.77568365958918	0.0
36	0.0010554883414715882	0.0	0.0	33.35593837531318	0.0
37	0.0010554883414715882	0.0	0.0	34.02775670465985	0.0
38	0.0010554883414715882	0.0	0.0	34.647064489018305	0.0
39	0.0010554883414715882	0.0	0.0	35.351075212779854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	25	3.889543E-5	45.000004	12
CGACGTC	25	3.889543E-5	45.000004	42
TTAGCTA	25	3.889543E-5	45.000004	35
CTATCCG	25	3.889543E-5	45.000004	1
CGCATTG	25	3.889543E-5	45.000004	27
TAAACGG	25	3.889543E-5	45.000004	2
CGTTATG	25	3.889543E-5	45.000004	42
TACGCTT	25	3.889543E-5	45.000004	18
CTAGTAT	25	3.889543E-5	45.000004	19
TACGACA	25	3.889543E-5	45.000004	32
AACGCCA	65	0.0	45.000004	33
AGTTCGC	25	3.889543E-5	45.000004	31
TCCCGTA	25	3.889543E-5	45.000004	27
GCAGCTA	55	1.8189894E-12	45.000004	9
CCGCCTA	25	3.889543E-5	45.000004	31
TTCGATG	65	0.0	45.000004	1
TGTCGCG	45	3.8380676E-10	45.000004	1
CTAGGCA	45	3.8380676E-10	45.000004	4
ATCGCGC	25	3.889543E-5	45.000004	23
GTCGGTT	25	3.889543E-5	45.000004	12
>>END_MODULE