##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935331.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1024991
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31205542292567	33.0	31.0	34.0	30.0	34.0
2	32.4656509179105	34.0	31.0	34.0	30.0	34.0
3	32.54257452016652	34.0	31.0	34.0	30.0	34.0
4	35.99150626688429	37.0	35.0	37.0	35.0	37.0
5	31.69074167480495	37.0	35.0	37.0	0.0	37.0
6	33.27944733173267	37.0	35.0	37.0	17.0	37.0
7	35.13033285170309	37.0	35.0	37.0	32.0	37.0
8	35.63346897680077	37.0	35.0	37.0	35.0	37.0
9	37.69927735950852	39.0	37.0	39.0	35.0	39.0
10	37.5094191070946	39.0	37.0	39.0	35.0	39.0
11	37.45710840387867	39.0	37.0	39.0	35.0	39.0
12	37.205192045588696	39.0	37.0	39.0	34.0	39.0
13	37.074045528204636	39.0	37.0	39.0	33.0	39.0
14	38.2790756211518	40.0	37.0	41.0	34.0	41.0
15	38.3371385699972	40.0	37.0	41.0	34.0	41.0
16	38.476114424419336	40.0	38.0	41.0	34.0	41.0
17	38.42016954295208	40.0	37.0	41.0	34.0	41.0
18	38.4342262517427	40.0	37.0	41.0	34.0	41.0
19	38.439333613661	40.0	37.0	41.0	34.0	41.0
20	38.25613200506151	40.0	37.0	41.0	34.0	41.0
21	38.27374386701932	40.0	37.0	41.0	34.0	41.0
22	38.24900218636066	40.0	37.0	41.0	34.0	41.0
23	38.119492756521765	40.0	37.0	41.0	34.0	41.0
24	38.13649583264633	40.0	37.0	41.0	34.0	41.0
25	38.07784751280743	40.0	36.0	41.0	34.0	41.0
26	37.985873046690166	40.0	36.0	41.0	34.0	41.0
27	37.87207107184356	40.0	36.0	41.0	33.0	41.0
28	37.850083561709326	40.0	36.0	41.0	33.0	41.0
29	37.843655212582355	40.0	36.0	41.0	33.0	41.0
30	37.832232673262496	40.0	36.0	41.0	33.0	41.0
31	37.65891310265163	40.0	36.0	41.0	33.0	41.0
32	37.562729819091096	40.0	36.0	41.0	33.0	41.0
33	37.53368956410349	40.0	36.0	41.0	33.0	41.0
34	37.44893760042771	40.0	35.0	41.0	33.0	41.0
35	37.3929293037695	40.0	35.0	41.0	33.0	41.0
36	37.35598166227801	40.0	35.0	41.0	33.0	41.0
37	37.29320647693492	40.0	35.0	41.0	33.0	41.0
38	37.3457230356169	40.0	35.0	41.0	33.0	41.0
39	37.300546053575104	40.0	35.0	41.0	33.0	41.0
40	37.259260813021776	39.0	35.0	41.0	33.0	41.0
41	37.17580056800499	39.0	35.0	41.0	32.0	41.0
42	37.10589946643434	39.0	35.0	41.0	32.0	41.0
43	37.03411932397455	39.0	35.0	41.0	32.0	41.0
44	37.00757177380094	39.0	35.0	41.0	32.0	41.0
45	36.99298335302456	39.0	35.0	41.0	32.0	41.0
46	36.90436208708174	39.0	35.0	41.0	32.0	41.0
47	36.79992312127619	39.0	35.0	41.0	32.0	41.0
48	36.81726766381363	39.0	35.0	41.0	32.0	41.0
49	36.76911114341492	39.0	35.0	41.0	32.0	41.0
50	36.662236058658074	39.0	35.0	41.0	32.0	41.0
51	36.10182918679286	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	10.0
12	12.0
13	16.0
14	26.0
15	30.0
16	56.0
17	70.0
18	142.0
19	278.0
20	638.0
21	1412.0
22	2335.0
23	3232.0
24	4236.0
25	5146.0
26	5613.0
27	6274.0
28	7594.0
29	9387.0
30	12475.0
31	16915.0
32	23745.0
33	36105.0
34	61194.0
35	90116.0
36	82642.0
37	128632.0
38	206672.0
39	319568.0
40	406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.49644533464196	4.5567229370794475	51.69899052772171	15.247841200556882
2	18.413820218909237	10.041649146187625	50.24180700123221	21.30272363367093
3	20.740572356245078	9.887696574896756	48.72403757691531	20.647693491942857
4	19.456756205664245	4.729797627491363	51.72464928960352	24.088796877240874
5	19.53783008826419	17.311566638146093	40.55177069847443	22.598832575115292
6	19.860759753012466	11.304099255505658	51.10269260900827	17.732448382473603
7	78.96586409051397	2.7598291106946307	12.761380343827408	5.512926454963995
8	79.90060400530346	7.547676028374884	7.118989337467354	5.432730628854302
9	74.73568060597606	5.833904883067266	9.228666398046421	10.20174811291026
10	39.42629740163572	28.250882202868123	16.16989807715385	16.15292231834231
11	32.00154928189613	22.769273096056452	25.203050563370798	20.026127058676614
12	27.2218975581249	20.317251566111313	29.288452288849363	23.172398586914422
13	23.901380597488174	24.139138782681993	31.687009934721377	20.272470685108456
14	19.59148909600182	29.00513272799469	29.051474598313547	22.35190357768995
15	16.17458104510186	23.02751926602282	38.17672545417472	22.621174234700597
16	19.773051665819505	22.960591849099163	31.216469217778496	26.049887267302836
17	20.65774236066463	24.250164147782762	28.983376439402882	26.10871705214973
18	20.212762843771312	22.303025099732583	30.76787991309192	26.716332143404188
19	21.634238739657228	24.55309363691974	28.540153035490068	25.272514587932964
20	26.196230015678186	24.83621807410992	29.25674469336804	19.710807216843858
21	25.631834816110583	27.289703031538814	26.889504395648352	20.18895775670225
22	22.73112641964661	24.068796701629573	28.508055192679745	24.69202168604407
23	22.653272077510923	25.980520804572922	26.75711298928478	24.609094128631373
24	22.72907762116936	24.878267223809768	28.105710196479773	24.2869449585411
25	20.42886230220558	28.47020120176665	26.725990764796958	24.37494573123081
26	19.960370383739956	27.391264898911306	28.146978851521627	24.501385865827114
27	22.98400668883922	26.43925653981352	26.472915371939855	24.10382139940741
28	18.423576402134263	26.453988376483306	30.606902889879034	24.515532331503398
29	19.611879518942118	24.253481250079272	31.640570502570263	24.494068728408347
30	21.63531191981198	25.955057166355605	29.847384025810957	22.562246888021456
31	22.61805225606859	25.104805798294816	26.780918076353842	25.49622386928275
32	24.653387200472977	26.925114464419686	25.803250955374242	22.618247379733088
33	24.959536230074214	23.821087209546228	26.33076778235126	24.888608778028296
34	20.60837607354601	25.071049404336232	28.721422919811	25.59915160230675
35	22.57668603919449	24.64509444473171	27.99985560848827	24.77836390758553
36	24.291042555495608	27.035749582191453	25.522175316661315	23.15103254565162
37	21.857850459174763	25.549980438852632	29.623967429957922	22.968201672014683
38	20.45032590530063	25.25007536651541	27.855561658590172	26.444037069593783
39	22.227121994241898	25.289100099415506	30.4893408820175	21.99443702432509
40	21.551018496747776	22.726541013530852	30.442511202537386	25.279929287183982
41	20.143786628370393	24.598167203419347	27.730682513309873	27.527363654900384
42	22.277268776018524	23.356985573531865	27.386191683634298	26.979553966815317
43	23.496303869985198	24.210651605721417	26.535647630076753	25.757396894216633
44	22.188487508670807	22.98976283694198	30.080751928553518	24.74099772583369
45	21.22096681824523	23.221179503039536	27.365898822526248	28.191954856188982
46	22.455806929036452	25.098561841030797	29.53255199313945	22.913079236793298
47	19.322511124487924	24.44821466725074	32.47501685380652	23.75425735445482
48	20.619302998758037	23.128788447898565	30.331583399268876	25.920325154074526
49	21.318235964998717	21.046526262181814	33.35512214253589	24.280115630283582
50	21.81102077969465	22.213365775894616	29.295671864435885	26.679941579974848
51	19.813832511700102	22.587710526238766	28.721813167140002	28.876643794921126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	411.0
1	644.5
2	878.0
3	1287.0
4	1696.0
5	1335.5
6	975.0
7	1102.5
8	1230.0
9	1361.0
10	1492.0
11	1623.0
12	1754.0
13	1804.0
14	1854.0
15	1857.5
16	1861.0
17	1894.5
18	1928.0
19	2235.0
20	2542.0
21	2854.5
22	3167.0
23	3803.5
24	4440.0
25	5252.0
26	7367.5
27	8671.0
28	9737.0
29	10803.0
30	13057.5
31	15312.0
32	17653.5
33	19995.0
34	23861.0
35	27727.0
36	29367.5
37	31008.0
38	33648.5
39	36289.0
40	41012.5
41	45736.0
42	52923.0
43	60110.0
44	66664.0
45	73218.0
46	92912.5
47	112607.0
48	112575.5
49	112544.0
50	106564.0
51	100584.0
52	86345.0
53	72106.0
54	64056.0
55	56006.0
56	52280.5
57	48555.0
58	45671.0
59	42787.0
60	37939.5
61	33092.0
62	30166.5
63	27241.0
64	24247.0
65	21253.0
66	17714.0
67	14175.0
68	11720.0
69	9265.0
70	7575.0
71	5885.0
72	5095.5
73	4306.0
74	3670.0
75	2364.0
76	1694.0
77	1088.5
78	483.0
79	329.5
80	176.0
81	103.5
82	31.0
83	17.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	1.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1024991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.930919821133283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.14580203394384	15.626459225871875
2	12.483404754231644	5.7251190702807975
3	5.220582449853577	3.591382727316239
4	2.8522678479233	2.6162050131650227
5	1.8059926979403138	2.070653687701076
6	1.2066425347853136	1.660165393075863
7	0.9029356853778385	1.449360206352747
8	0.6799553546696975	1.2473601375904861
9	0.5554834575842115	1.14639719650465
>10	4.114589379270284	20.188333695691764
>50	1.7047408317964827	28.274541750317645
>100	0.31339679904060097	10.078145373502565
>500	0.007748821954300574	1.2155807168886268
>1k	0.004735391194294796	2.4922662302490792
>5k	0.0017219604342890162	2.618029575491577
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC	7897	0.770445789280101	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG	7749	0.7560066381070663	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC	5639	0.5501511720590717	No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	5236	0.5108337536622273	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	4481	0.4371745703133003	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	4069	0.39697909542620374	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTTTCGT	3574	0.3486859884623377	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	3529	0.3442957060110772	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTA	1676	0.16351363085139287	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCT	1625	0.1585379774066309	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTC	1603	0.15639161709712573	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT	1403	0.13687925064707884	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG	1177	0.11483027655852587	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG	1083	0.10565946432700385	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC	1027	0.10019600172099072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.75618322502344E-5	0.0	0.0	0.5267363323190155	0.0
2	9.75618322502344E-5	0.0	0.0	1.7664545347227438	0.0
3	9.75618322502344E-5	0.0	0.0	2.2654833066826927	0.0
4	9.75618322502344E-5	0.0	0.0	2.969782173697135	0.0
5	9.75618322502344E-5	0.0	0.0	4.4697953445444885	0.0
6	9.75618322502344E-5	0.0	0.0	5.11545955037654	0.0
7	9.75618322502344E-5	0.0	0.0	5.864051489232589	0.0
8	9.75618322502344E-5	0.0	0.0	6.878304297306025	0.0
9	9.75618322502344E-5	0.0	0.0	7.266307704165207	0.0
10	9.75618322502344E-5	0.0	0.0	8.930029629528454	0.0
11	9.75618322502344E-5	0.0	0.0	10.447116121019599	0.0
12	9.75618322502344E-5	0.0	0.0	12.713477484192545	0.0
13	9.75618322502344E-5	0.0	0.0	13.244701660795071	0.0
14	9.75618322502344E-5	0.0	0.0	13.453288858146072	0.0
15	2.926854967507032E-4	0.0	0.0	13.905487950625908	0.0
16	3.902473290009376E-4	0.0	0.0	14.572518197720761	0.0
17	3.902473290009376E-4	0.0	0.0	15.444428292541104	0.0
18	0.001268303819253047	0.0	0.0	16.37536329587284	0.0
19	0.001268303819253047	0.0	0.0	17.495860939266784	0.0
20	0.0013658656515032814	0.0	0.0	18.26084326594087	0.0
21	0.0013658656515032814	0.0	0.0	19.141338801999236	0.0
22	0.0015609893160037503	0.0	0.0	20.014224515142086	0.0
23	0.0015609893160037503	0.0	0.0	20.796767971621215	0.0
24	0.0015609893160037503	0.0	0.0	21.449944438536534	0.0
25	0.0015609893160037503	0.0	0.0	22.01121765947213	0.0
26	0.0015609893160037503	0.0	0.0	22.536002755146143	0.0
27	0.0015609893160037503	0.0	0.0	23.065373256935914	0.0
28	0.0015609893160037503	0.0	0.0	23.568304502185875	0.0
29	0.0015609893160037503	0.0	0.0	24.12499231700571	0.0
30	0.0015609893160037503	0.0	0.0	24.768607724360507	0.0
31	0.0015609893160037503	0.0	0.0	25.334466351411866	0.0
32	0.0015609893160037503	0.0	0.0	25.853983108144366	0.0
33	0.0015609893160037503	0.0	0.0	26.35613385873632	0.0
34	0.0016585511482539847	0.0	0.0	26.876528671959072	0.0
35	0.0016585511482539847	0.0	0.0	27.424045674547386	0.0
36	0.0016585511482539847	0.0	0.0	27.972343171793703	0.0
37	0.0016585511482539847	0.0	0.0	28.480640317817425	0.0
38	0.0016585511482539847	0.0	0.0	29.013035236406953	0.0
39	0.0016585511482539847	0.0	0.0	29.54474722217073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.032928E-4	45.000004	34
GGTACCG	20	7.032928E-4	45.000004	24
GGTCGTC	35	1.2117562E-7	45.000004	7
ACTTCGA	20	7.032928E-4	45.000004	36
TCGATTG	35	1.2117562E-7	45.000004	1
ACGGGTA	70	0.0	45.000004	5
AACGGCA	20	7.032928E-4	45.000004	5
GTCGTCA	35	1.2117562E-7	45.000004	8
TTAACGA	20	7.032928E-4	45.000004	32
CGCCGTT	20	7.032928E-4	45.000004	26
GTGCGAC	20	7.032928E-4	45.000004	14
TAGTCCG	20	7.032928E-4	45.000004	26
TCCGGAC	25	3.89051E-5	45.0	14
CGTATAG	25	3.89051E-5	45.0	32
CTATCGT	85	0.0	45.0	45
ACGTTAG	25	3.89051E-5	45.0	38
GCGCGTA	25	3.89051E-5	45.0	22
GCGAAAC	25	3.89051E-5	45.0	27
CGTTGCG	25	3.89051E-5	45.0	13
CGTTATC	25	3.89051E-5	45.0	26
>>END_MODULE