FastQCFastQC Report
Sat 14 Jan 2017
SRR2935330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604359
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC34070.5637377783734503TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT30720.5083071485656704TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC26270.43467541643294794TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC26000.4302078731350075TruSeq Adapter, Index 16 (95% over 21bp)
GTTGTAGGGGAATGAATGCACAATATTCTTTTTATATCAAAACCTAAAGCA15290.2529953223167025No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC12400.20517606257208051No Hit
TCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG11840.1959100468430188No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG11370.1881332122132706No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9050.1497454327643007No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC8860.1466016059990833No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8540.14130673986819092No Hit
TTCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT8390.1388247713693351No Hit
TGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG7700.12740771627459838No Hit
GCTAGAGGGGCTGGCAGTTTGCAGCAGAACGGATGTGAAGAAAATAATTCT7500.12409842494279062No Hit
GCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG6310.10440814151853452No Hit
TTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG6110.10109885018672676No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6090.100767921053546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.0306275E-445.00000430
CTTAAGC207.0306275E-445.00000444
TATCAGT207.0306275E-445.00000423
CTTCGAA207.0306275E-445.00000442
GTGCACG207.0306275E-445.00000421
AGCGTGA207.0306275E-445.00000425
TAATACG207.0306275E-445.0000044
ACACGTA207.0306275E-445.00000425
ATTAGGT453.8380676E-1045.00000416
CTCCGAT207.0306275E-445.00000438
CACGGGT207.0306275E-445.0000044
ACGCAGA207.0306275E-445.00000423
TCCGAAG207.0306275E-445.00000421
CGTTGTT207.0306275E-445.00000443
CACATCG207.0306275E-445.00000411
ATCAGGC800.045.00000415
ATTACGG207.0306275E-445.0000042
ATAGCGG453.8380676E-1045.0000042
TGTATCG207.0306275E-445.0000042
CGATTTA453.8380676E-1045.00000422
Produced by FastQC (version 0.11.5)