##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604359
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32136528123185	33.0	31.0	34.0	30.0	34.0
2	32.46641813888765	34.0	31.0	34.0	31.0	34.0
3	32.53446544189794	34.0	31.0	34.0	31.0	34.0
4	35.98636902900429	37.0	35.0	37.0	35.0	37.0
5	31.51036718241972	37.0	35.0	37.0	0.0	37.0
6	33.31606379651829	37.0	35.0	37.0	17.0	37.0
7	35.203162358796675	37.0	35.0	37.0	32.0	37.0
8	35.7702789236199	37.0	35.0	37.0	35.0	37.0
9	37.82687111468515	39.0	38.0	39.0	35.0	39.0
10	37.55700833445022	39.0	37.0	39.0	35.0	39.0
11	37.474747625169805	39.0	37.0	39.0	35.0	39.0
12	37.21382985940476	39.0	37.0	39.0	34.0	39.0
13	37.06169511829889	39.0	37.0	39.0	33.0	39.0
14	38.1937226052727	40.0	37.0	41.0	33.0	41.0
15	38.32305467445674	40.0	37.0	41.0	34.0	41.0
16	38.408273890187786	40.0	37.0	41.0	34.0	41.0
17	38.420910419138295	40.0	37.0	41.0	34.0	41.0
18	38.38880532928276	40.0	37.0	41.0	34.0	41.0
19	38.406425650978974	40.0	37.0	41.0	34.0	41.0
20	38.30244440804224	40.0	37.0	41.0	34.0	41.0
21	38.254582127510304	40.0	37.0	41.0	34.0	41.0
22	38.21659146302115	40.0	37.0	41.0	34.0	41.0
23	38.15070016331353	40.0	37.0	41.0	34.0	41.0
24	38.14287699860513	40.0	37.0	41.0	34.0	41.0
25	38.04327229345472	40.0	36.0	41.0	34.0	41.0
26	37.95132197915478	40.0	36.0	41.0	34.0	41.0
27	37.90111175642292	40.0	36.0	41.0	34.0	41.0
28	37.84836330723957	40.0	36.0	41.0	34.0	41.0
29	37.81803696147489	40.0	36.0	41.0	34.0	41.0
30	37.795350114749674	40.0	36.0	41.0	34.0	41.0
31	37.5752607307908	39.0	36.0	41.0	33.0	41.0
32	37.57777082826598	39.0	35.0	41.0	33.0	41.0
33	37.58835063265377	39.0	35.0	41.0	33.0	41.0
34	37.56611054025836	39.0	35.0	41.0	33.0	41.0
35	37.478444103587435	39.0	35.0	41.0	33.0	41.0
36	37.464685724875444	39.0	35.0	41.0	33.0	41.0
37	37.404084327361716	39.0	35.0	41.0	33.0	41.0
38	37.407415129087184	39.0	35.0	41.0	33.0	41.0
39	37.409950046247346	39.0	35.0	41.0	33.0	41.0
40	37.31343952849217	39.0	35.0	41.0	33.0	41.0
41	37.27269553361496	39.0	35.0	41.0	33.0	41.0
42	37.26432799048248	39.0	35.0	41.0	33.0	41.0
43	37.2317447080295	39.0	35.0	41.0	33.0	41.0
44	37.18309812545193	39.0	35.0	41.0	33.0	41.0
45	37.16089939919816	39.0	35.0	41.0	33.0	41.0
46	37.02466745758729	39.0	35.0	41.0	33.0	41.0
47	36.948391601680456	39.0	35.0	40.0	33.0	41.0
48	36.94024909035854	39.0	35.0	40.0	33.0	41.0
49	36.968881078961346	39.0	35.0	40.0	33.0	41.0
50	36.87418074356467	39.0	35.0	40.0	32.0	41.0
51	36.207303936898434	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	3.0
12	8.0
13	7.0
14	8.0
15	16.0
16	14.0
17	28.0
18	61.0
19	108.0
20	188.0
21	345.0
22	569.0
23	875.0
24	1159.0
25	1731.0
26	2384.0
27	3101.0
28	3962.0
29	5331.0
30	7048.0
31	9983.0
32	14011.0
33	22375.0
34	37868.0
35	60898.0
36	53716.0
37	82329.0
38	129771.0
39	166283.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.26481445630825	4.3300422431038506	57.43424024462281	14.97090305596508
2	18.018594907993428	5.20948641453176	55.89177955486723	20.880139122607588
3	19.699880369118354	5.229342162522607	53.03586113551714	22.034916332841902
4	18.533520639222715	4.888816084479589	51.81092694904849	24.766736327249202
5	14.78310077288499	17.124424390138973	45.24976049003986	22.842714346936173
6	19.454000023165037	7.549155386119839	53.53771516598578	19.45912942472934
7	81.26179969190498	2.0267754761656565	11.066104748998526	5.645320082930841
8	83.75353060018962	2.7894016635807524	7.257441355220987	6.199626381008639
9	78.10225379286153	4.713423643893779	10.30347856158343	6.880844001661264
10	40.21186083106233	25.762336624423565	16.458925903312434	17.56687664120167
11	29.44938356175717	24.186783021349893	24.432001509036848	21.931831907856093
12	25.168318830364072	21.24631220847212	30.920363558745713	22.6650054024181
13	22.010262112419937	24.948581885932036	33.00124594818642	20.0399100534616
14	18.26745361614537	28.303375973552143	32.92182295622304	20.507347454079447
15	14.559392678854788	24.919625586778718	39.11152146323625	21.40946027113024
16	17.277313649668493	23.38295615685379	34.534937015912725	24.804793177564992
17	18.111089600717452	23.647864927965003	28.57539972102674	29.665645750290803
18	20.55169195792567	22.701242142501393	32.941182310514115	23.805883589058823
19	22.00380899432291	23.696842439675756	29.47569242784504	24.823656138156295
20	25.22325306647208	24.53690604425515	29.19853927880614	21.041301610466626
21	23.312633716052876	27.15058433811691	27.6190145261343	21.91776741969591
22	22.261933718203917	24.2607456826158	27.630762510362217	25.84655808881807
23	20.019723376337577	26.421051064019895	27.53264202237412	26.02658353726841
24	21.445035152947174	22.888878961014893	31.16756762123175	24.498518264806183
25	20.02187441570325	26.26882366275674	28.090588540916904	25.618713380623102
26	17.837576672143545	29.50448326243177	28.242650477613473	24.415289587811216
27	20.246575297132996	28.969370854078452	28.672196492482115	22.111857356306434
28	16.009027746753173	28.34805140653155	31.683486139860577	23.9594347068547
29	17.280457476433707	26.18410580466246	31.21902710144136	25.316409617462472
30	20.268582084489516	26.86217959854987	29.38220494772147	23.487033369239143
31	22.499540835827712	28.50772471329127	24.445238674364077	24.547495776516936
32	22.390665151011234	29.688148931347097	23.3225615900483	24.59862432759337
33	21.453142916710103	27.38405484157595	24.694593776215793	26.468208465498154
34	18.713711552239644	28.347224083698595	25.5310171603302	27.408047203731556
35	19.17701233869273	27.109218196469314	26.97436457469815	26.739404890139802
36	22.484318095701397	26.728649693311425	26.94027887398053	23.846753337006646
37	18.3857607812575	27.37081767624872	29.55958296310637	24.68383857938742
38	19.072604197174194	29.393622002816205	24.563380374909617	26.97039342509998
39	21.682476806004377	25.797580577107315	25.506362939908232	27.013579676980072
40	22.488785638999335	25.037601822757665	26.612824496698156	25.86078804154484
41	17.884899538188396	27.01738536201165	25.349998924480317	29.74771617531964
42	19.727182022605767	27.13403788145787	25.908441836722872	27.230338259213482
43	20.94433937444466	24.271500879444172	27.09548463744231	27.688675108668853
44	20.12380058872293	24.625595051947602	27.756184651837735	27.49441970749174
45	20.34783961188631	25.498917034411665	25.50884490840709	28.644398445294932
46	23.079494141727018	26.053223332489463	27.109218196469314	23.758064329314198
47	17.95869673488771	26.936638653515544	29.852786175104534	25.25187843649222
48	18.239490104391596	25.94451311223958	29.1631298615558	26.65286692181303
49	21.293800539083556	21.724504805918336	30.64635423647203	26.335340418526076
50	20.391687722032763	23.145845432929764	27.790270352555353	28.672196492482115
51	19.522005960033688	24.92574777574256	25.069701948676197	30.48254431554755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	231.0
1	298.5
2	366.0
3	621.0
4	876.0
5	712.5
6	549.0
7	646.5
8	744.0
9	972.0
10	1200.0
11	1315.5
12	1431.0
13	1472.5
14	1514.0
15	1599.0
16	1684.0
17	1587.0
18	1490.0
19	1613.5
20	1737.0
21	1991.5
22	2246.0
23	2496.0
24	2746.0
25	3139.5
26	3196.5
27	2860.0
28	4129.5
29	5399.0
30	7594.5
31	9790.0
32	10158.0
33	10526.0
34	11841.5
35	13157.0
36	14567.0
37	15977.0
38	17439.5
39	18902.0
40	24502.0
41	30102.0
42	34597.0
43	39092.0
44	45009.5
45	50927.0
46	60774.0
47	70621.0
48	76368.0
49	82115.0
50	74205.5
51	66296.0
52	53909.5
53	41523.0
54	35666.5
55	29810.0
56	26461.0
57	23112.0
58	20763.5
59	18415.0
60	17536.5
61	16658.0
62	13820.5
63	10983.0
64	10865.0
65	10747.0
66	8601.5
67	6456.0
68	5027.5
69	3599.0
70	3148.5
71	2698.0
72	2236.5
73	1775.0
74	1557.5
75	948.5
76	557.0
77	446.0
78	335.0
79	246.5
80	158.0
81	111.5
82	65.0
83	40.0
84	15.0
85	8.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	604359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.764198392701765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.85897858285995	16.557090099593417
2	12.619299725382113	6.250136839526591
3	5.798664177271368	4.307978102958027
4	3.1754400900118065	3.1454891349276455
5	2.0456666802476167	2.532964775749579
6	1.4361005957771773	2.133832803942193
7	1.0009446138258278	1.735131369782234
8	0.7245922709527645	1.4355157401354055
9	0.6086664524631173	1.3565823105400756
>10	4.1005121354836644	20.5976091024815
>50	1.250648229806155	22.67341421159974
>100	0.36708903585097646	13.082098333620873
>500	0.00803844604053233	1.4059013121496924
>1k	0.005358964027021554	2.786255862993066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	3407	0.5637377783734503	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	3072	0.5083071485656704	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	2627	0.43467541643294794	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	2600	0.4302078731350075	TruSeq Adapter, Index 16 (95% over 21bp)
GTTGTAGGGGAATGAATGCACAATATTCTTTTTATATCAAAACCTAAAGCA	1529	0.2529953223167025	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	1240	0.20517606257208051	No Hit
TCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	1184	0.1959100468430188	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	1137	0.1881332122132706	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	905	0.1497454327643007	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	886	0.1466016059990833	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	854	0.14130673986819092	No Hit
TTCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	839	0.1388247713693351	No Hit
TGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	770	0.12740771627459838	No Hit
GCTAGAGGGGCTGGCAGTTTGCAGCAGAACGGATGTGAAGAAAATAATTCT	750	0.12409842494279062	No Hit
GCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	631	0.10440814151853452	No Hit
TTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	611	0.10109885018672676	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	609	0.100767921053546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5313067233217342	0.0
2	0.0	0.0	0.0	2.0686380115130247	0.0
3	0.0	0.0	0.0	2.923758891652147	0.0
4	0.0	0.0	0.0	3.900661692801795	0.0
5	0.0	0.0	0.0	5.642010791599033	0.0
6	0.0	0.0	0.0	6.803572049063553	0.0
7	0.0	0.0	0.0	7.862710739808624	0.0
8	0.0	0.0	0.0	9.544492594633322	0.0
9	0.0	0.0	0.0	10.341535411899219	0.0
10	0.0	0.0	0.0	11.929002463767397	0.0
11	0.0	0.0	0.0	14.383007450869433	0.0
12	0.0	0.0	0.0	16.693720123304196	0.0
13	0.0	0.0	0.0	17.362527901462542	0.0
14	0.0	0.0	0.0	17.583257633294117	0.0
15	0.0	0.0	0.0	17.961840561652924	0.0
16	0.0	0.0	0.0	18.833673363017677	0.0
17	0.0	0.0	0.0	20.08210351794215	0.0
18	0.0	0.0	0.0	21.571946475522	0.0
19	0.0	0.0	0.0	22.59733039468263	0.0
20	0.0	0.0	0.0	23.541636676213972	0.0
21	0.0	0.0	0.0	24.76690179181579	0.0
22	0.0	0.0	0.0	25.973469411392898	0.0
23	0.0	0.0	0.0	27.065204621756273	0.0
24	0.0	0.0	0.0	27.901958935003865	0.0
25	0.0	0.0	0.0	28.58350748478967	0.0
26	0.0	0.0	0.0	29.24023634958692	0.0
27	3.30929133180775E-4	0.0	0.0	29.823829875951215	0.0
28	3.30929133180775E-4	0.0	0.0	30.502234598971803	0.0
29	3.30929133180775E-4	0.0	0.0	31.184775936157152	0.0
30	3.30929133180775E-4	0.0	0.0	31.93581960391092	0.0
31	3.30929133180775E-4	0.0	0.0	32.726740232212975	0.0
32	3.30929133180775E-4	0.0	0.0	33.43476311265324	0.0
33	3.30929133180775E-4	0.0	0.0	34.114160623073374	0.0
34	3.30929133180775E-4	0.0	0.0	34.75632860601067	0.0
35	3.30929133180775E-4	0.0	0.0	35.410906431442235	0.0
36	3.30929133180775E-4	0.0	0.0	36.04116096558502	0.0
37	3.30929133180775E-4	0.0	0.0	36.70417748391271	0.0
38	3.30929133180775E-4	0.0	0.0	37.36553935657449	0.0
39	3.30929133180775E-4	0.0	0.0	38.001916079681116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.0306275E-4	45.000004	30
CTTAAGC	20	7.0306275E-4	45.000004	44
TATCAGT	20	7.0306275E-4	45.000004	23
CTTCGAA	20	7.0306275E-4	45.000004	42
GTGCACG	20	7.0306275E-4	45.000004	21
AGCGTGA	20	7.0306275E-4	45.000004	25
TAATACG	20	7.0306275E-4	45.000004	4
ACACGTA	20	7.0306275E-4	45.000004	25
ATTAGGT	45	3.8380676E-10	45.000004	16
CTCCGAT	20	7.0306275E-4	45.000004	38
CACGGGT	20	7.0306275E-4	45.000004	4
ACGCAGA	20	7.0306275E-4	45.000004	23
TCCGAAG	20	7.0306275E-4	45.000004	21
CGTTGTT	20	7.0306275E-4	45.000004	43
CACATCG	20	7.0306275E-4	45.000004	11
ATCAGGC	80	0.0	45.000004	15
ATTACGG	20	7.0306275E-4	45.000004	2
ATAGCGG	45	3.8380676E-10	45.000004	2
TGTATCG	20	7.0306275E-4	45.000004	2
CGATTTA	45	3.8380676E-10	45.000004	22
>>END_MODULE