##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1064528
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55987630198548	34.0	31.0	34.0	31.0	34.0
2	32.720016758601	34.0	31.0	34.0	31.0	34.0
3	32.750169089023494	34.0	31.0	34.0	31.0	34.0
4	36.15148215922925	37.0	35.0	37.0	35.0	37.0
5	31.82010055160597	37.0	35.0	37.0	0.0	37.0
6	33.584418634361896	37.0	35.0	37.0	17.0	37.0
7	35.36934397216419	37.0	35.0	37.0	32.0	37.0
8	35.902373634136445	37.0	35.0	37.0	35.0	37.0
9	37.96979506410353	39.0	38.0	39.0	35.0	39.0
10	37.76841755219215	39.0	37.0	39.0	35.0	39.0
11	37.68210324200021	39.0	37.0	39.0	35.0	39.0
12	37.503731231118394	39.0	37.0	39.0	35.0	39.0
13	37.43151612733531	39.0	37.0	39.0	35.0	39.0
14	38.68105582943802	40.0	38.0	41.0	35.0	41.0
15	38.758728751146045	40.0	38.0	41.0	35.0	41.0
16	38.8278241624457	40.0	38.0	41.0	35.0	41.0
17	38.805427381900714	40.0	38.0	41.0	35.0	41.0
18	38.79382035982144	40.0	38.0	41.0	35.0	41.0
19	38.80986409000045	40.0	38.0	41.0	35.0	41.0
20	38.74528899192882	40.0	38.0	41.0	35.0	41.0
21	38.689585431289736	40.0	38.0	41.0	35.0	41.0
22	38.66537470127606	40.0	38.0	41.0	35.0	41.0
23	38.623184171764386	40.0	38.0	41.0	35.0	41.0
24	38.6042142620955	40.0	38.0	41.0	35.0	41.0
25	38.51867306449431	40.0	38.0	41.0	35.0	41.0
26	38.50033066297927	40.0	38.0	41.0	35.0	41.0
27	38.441418168427695	40.0	37.0	41.0	35.0	41.0
28	38.42823673966303	40.0	37.0	41.0	35.0	41.0
29	38.384912374310495	40.0	37.0	41.0	35.0	41.0
30	38.347735334345366	40.0	37.0	41.0	34.0	41.0
31	38.16762922158929	40.0	37.0	41.0	34.0	41.0
32	38.20305900831166	40.0	37.0	41.0	34.0	41.0
33	38.20419848045331	40.0	37.0	41.0	34.0	41.0
34	38.16678659462222	40.0	37.0	41.0	34.0	41.0
35	38.120637503193905	40.0	37.0	41.0	34.0	41.0
36	38.09114555934649	40.0	37.0	41.0	34.0	41.0
37	38.082153780830566	40.0	37.0	41.0	34.0	41.0
38	38.070517637863915	40.0	37.0	41.0	34.0	41.0
39	38.036201959929656	40.0	37.0	41.0	34.0	41.0
40	37.94671253362993	40.0	36.0	41.0	34.0	41.0
41	37.89894394510995	40.0	36.0	41.0	34.0	41.0
42	37.900655501781074	40.0	36.0	41.0	34.0	41.0
43	37.84916507597733	40.0	36.0	41.0	34.0	41.0
44	37.7856683901222	40.0	36.0	41.0	34.0	41.0
45	37.744308275592566	40.0	36.0	41.0	34.0	41.0
46	37.64536019719538	40.0	35.0	41.0	34.0	41.0
47	37.59086844122466	39.0	35.0	41.0	33.0	41.0
48	37.577852343949615	39.0	35.0	41.0	33.0	41.0
49	37.56054138547788	39.0	35.0	41.0	34.0	41.0
50	37.494704695414306	39.0	35.0	41.0	33.0	41.0
51	36.88582733380428	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	13.0
11	8.0
12	13.0
13	11.0
14	12.0
15	24.0
16	35.0
17	49.0
18	87.0
19	150.0
20	269.0
21	423.0
22	748.0
23	1158.0
24	1667.0
25	2491.0
26	3336.0
27	4228.0
28	5012.0
29	6583.0
30	8756.0
31	12535.0
32	17830.0
33	29647.0
34	54231.0
35	85904.0
36	80304.0
37	134813.0
38	229094.0
39	384607.0
40	483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.191055566410654	5.061961733275217	56.08119279154705	16.665789908767078
2	19.368490072595552	4.911942194099169	53.615029383914745	22.10453834939053
3	19.484503930380413	5.0876069018381855	52.55850480212827	22.869384365653133
4	19.05933897464416	4.420550704161844	51.39432687538515	25.125783445808846
5	16.032081824057233	17.229983617152392	43.803826672478316	22.934107886312056
6	20.865867313964497	6.570611576210301	53.21738836366916	19.34613274615604
7	82.63286639712624	2.538589872694753	9.599183863646612	5.2293598665323975
8	84.54413599266529	2.906170622097305	7.064727278192777	5.484966107044625
9	78.84630559271339	4.719274645664558	9.71144018757609	6.7229795740459615
10	38.72054093457382	26.020640133467605	18.01098702899313	17.247831902965448
11	28.029605609246538	26.788116423428974	24.928700795094162	20.253577172230322
12	26.504516555694167	21.597271278914224	30.252092946357443	21.646119219034162
13	23.18201118242075	23.909469736822327	31.50081538484662	21.4077036959103
14	19.06741767243323	27.320089279004407	30.802571656170624	22.809921392391747
15	16.848969684216854	25.301448153547863	36.68912419400899	21.160457968226293
16	20.123190747448636	23.495483444305833	32.67062961237281	23.710696195872725
17	19.66899884267957	24.373431229615377	29.14136593870711	26.816203988997938
18	21.089816331745148	23.424278177746384	31.13520734071814	24.35069814979033
19	22.97778921738085	25.812378819533166	27.795887003441898	23.413944959644088
20	25.300602708430404	25.53441525258143	28.818218027144425	20.346764011843746
21	24.2048118978552	26.141914538649992	28.47787939819338	21.175394165301427
22	22.459343483684787	24.79173868606556	27.932473359084963	24.816444471164687
23	22.373295958396586	25.456540363428676	27.611298152796355	24.558865525378383
24	23.08675769918687	23.15880841086378	29.26583424766657	24.488599642282775
25	20.919693986442816	25.651086678790975	27.48899042580374	25.94022890896247
26	19.84466355041859	27.0273773916703	28.551151308373292	24.576807749537824
27	20.03000390783521	26.496719672944252	29.077863616551188	24.39541280266935
28	19.7706401334676	24.6981760930666	30.30357116017615	25.227612613289647
29	21.29309891332121	23.03124013647363	30.417236559301397	25.258424390903762
30	21.108322185982896	25.1503013542152	27.843137991673306	25.898238468128596
31	21.618877098582658	26.144920565734296	26.66656020320743	25.56964213247561
32	23.174496114709996	26.113451219695488	27.362549411570196	23.349503254024317
33	22.154137796281546	24.293395758495784	27.633561540889485	25.91890490433319
34	19.36050531315287	24.340740685073573	30.373837043271763	25.924916958501797
35	20.4234177024935	22.318154149068885	31.19579757413614	26.062630574301476
36	20.770144138998692	24.421809478003397	29.565215757593975	25.242830625403933
37	20.272458779853604	25.145792313588743	30.043268002344703	24.538480904212946
38	21.419445989208363	25.630138427547234	27.115209745539808	25.8352058377046
39	22.454270812980027	23.35560924653931	29.584754933641953	24.60536500683871
40	21.669885620669444	22.92753220206514	31.332759683164745	24.06982249410067
41	18.124934243157533	23.235931793245456	32.30708821186479	26.332045751732224
42	20.24878631656471	23.28769182210332	30.781999158312416	25.681522703019553
43	21.984860896096674	22.904047615469015	29.66798430853862	25.44310717989569
44	21.853722964543913	23.031615889859168	27.879867885109643	27.23479326048728
45	21.29939278252897	23.05275201779568	27.567053191649254	28.08080200802609
46	21.432879172741345	23.149508514571714	29.211631821802715	26.205980490884222
47	18.52605098221935	23.126493619707514	33.12810935926533	25.21934603880781
48	19.7839793786542	21.344765003832684	32.18055325928486	26.690702358228247
49	20.426329791231417	20.937354395563105	32.45560473749868	26.180711075706792
50	20.317361309425397	21.07422256624532	31.30842965145116	27.299986472878118
51	18.723039694587648	21.828265672673712	29.188710865284897	30.259983767453747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	520.5
2	751.0
3	1191.5
4	1632.0
5	1248.5
6	865.0
7	966.5
8	1068.0
9	1352.5
10	1637.0
11	1536.0
12	1435.0
13	1610.0
14	1785.0
15	1817.0
16	1849.0
17	1870.0
18	1891.0
19	2217.0
20	2543.0
21	2926.0
22	3309.0
23	4202.5
24	5096.0
25	6090.5
26	8392.0
27	9699.0
28	10711.0
29	11723.0
30	14327.0
31	16931.0
32	20142.0
33	23353.0
34	26161.5
35	28970.0
36	30224.0
37	31478.0
38	35041.0
39	38604.0
40	42408.0
41	46212.0
42	52912.5
43	59613.0
44	67998.0
45	76383.0
46	89042.5
47	101702.0
48	111648.0
49	121594.0
50	117176.5
51	112759.0
52	96225.5
53	79692.0
54	69945.0
55	60198.0
56	55035.0
57	49872.0
58	47494.0
59	45116.0
60	41812.0
61	38508.0
62	34631.5
63	30755.0
64	24410.0
65	18065.0
66	14956.5
67	11848.0
68	9802.0
69	7756.0
70	6174.0
71	4592.0
72	4015.0
73	3438.0
74	2838.5
75	1712.0
76	1185.0
77	870.5
78	556.0
79	421.5
80	287.0
81	155.0
82	23.0
83	42.0
84	61.0
85	67.5
86	74.0
87	39.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1064528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.10812916760418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.11658098094011	15.939470744088663
2	12.732948504322424	6.13935134453315
3	5.727988211048235	4.142732389873945
4	3.2340374407381445	3.1186636941673296
5	2.0405323793325807	2.4596709085814275
6	1.3856157368205118	2.0042761895960366
7	1.0426487359235606	1.7595417281459094
8	0.755650311907138	1.4573852259998137
9	0.6226753380457323	1.3510383731179303
>10	4.5414106398918905	24.170535886560824
>50	1.578359958056546	26.5625196637587
>100	0.21055273927940327	7.540276015719001
>500	0.008249267770274966	1.4164593306678839
>1k	0.0023569336486499903	1.4266871576192866
>5k	3.9282227477499846E-4	0.5113913475701393
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	5400	0.5072670704763049	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	3937	0.3698352697157801	TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	3733	0.3506718470533419	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	3282	0.308305652833932	TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	1782	0.16739813325718064	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	1210	0.11366539912524612	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	1121	0.10530488629702554	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3771624607337712	0.0
2	0.0	0.0	0.0	1.610854763801422	0.0
3	0.0	0.0	0.0	2.3077833556280343	0.0
4	0.0	0.0	0.0	3.0482054018306703	0.0
5	0.0	0.0	0.0	4.446007996032044	0.0
6	0.0	0.0	0.0	5.372427968075993	0.0
7	0.0	0.0	0.0	6.160101002510032	0.0
8	0.0	0.0	0.0	7.374442006222476	0.0
9	0.0	0.0	0.0	7.956296123728075	0.0
10	0.0	0.0	0.0	9.161619046187607	0.0
11	0.0	0.0	0.0	11.103418604301625	0.0
12	0.0	0.0	0.0	12.784163497813115	0.0
13	0.0	0.0	0.0	13.34882689793035	0.0
14	0.0	0.0	0.0	13.547412562187185	0.0
15	0.0	0.0	0.0	13.871875610599252	0.0
16	0.0	0.0	0.0	14.679651451159575	0.0
17	0.0	0.0	0.0	15.757218222536185	0.0
18	0.0	0.0	0.0	16.967895630739633	0.0
19	0.0	0.0	0.0	17.766277636661506	0.0
20	0.0	0.0	0.0	18.574147415568213	0.0
21	9.393834638450092E-5	0.0	0.0	19.66373829528204	0.0
22	9.393834638450092E-5	0.0	0.0	20.75257766822479	0.0
23	1.8787669276900185E-4	0.0	0.0	21.788341875460297	0.0
24	1.8787669276900185E-4	0.0	0.0	22.586817819728555	0.0
25	1.8787669276900185E-4	0.0	0.0	23.310143086889212	0.0
26	1.8787669276900185E-4	0.0	0.0	23.94770264380082	0.0
27	1.8787669276900185E-4	0.0	0.0	24.586013707483506	0.0
28	1.8787669276900185E-4	0.0	0.0	25.220473298964425	0.0
29	1.8787669276900185E-4	0.0	0.0	25.908759562923663	0.0
30	1.8787669276900185E-4	0.0	0.0	26.637909007560157	0.0
31	1.8787669276900185E-4	0.0	0.0	27.344137495678837	0.0
32	1.8787669276900185E-4	0.0	0.0	28.02612989043031	0.0
33	1.8787669276900185E-4	0.0	0.0	28.611177911712986	0.0
34	1.8787669276900185E-4	0.0	0.0	29.216610554161093	0.0
35	1.8787669276900185E-4	0.0	0.0	29.832282476365112	0.0
36	2.818150391535028E-4	0.0	0.0	30.50046593419807	0.0
37	3.757533855380037E-4	0.0	0.0	31.158879804007036	0.0
38	3.757533855380037E-4	0.0	0.0	31.79897569627102	0.0
39	3.757533855380037E-4	0.0	0.0	32.409950701155815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0330506E-4	45.000004	36
ACCGGTA	20	7.0330506E-4	45.000004	41
TCGCAAT	20	7.0330506E-4	45.000004	17
GTCTACG	40	6.8139343E-9	45.000004	1
CGACACG	20	7.0330506E-4	45.000004	16
TGACGCG	20	7.0330506E-4	45.000004	25
CCGTCCG	20	7.0330506E-4	45.000004	1
TCGGCAC	20	7.0330506E-4	45.000004	20
TTTCACG	65	0.0	45.000004	1
TCGTGCA	20	7.0330506E-4	45.000004	23
CGGATTG	20	7.0330506E-4	45.000004	24
GTCGTAC	20	7.0330506E-4	45.000004	22
AACGACG	40	6.8139343E-9	45.000004	11
TCCGTCG	30	2.1653505E-6	45.000004	1
TTACGCT	20	7.0330506E-4	45.000004	22
CACGTAT	20	7.0330506E-4	45.000004	34
TACAGCG	20	7.0330506E-4	45.000004	30
CCCGGTA	20	7.0330506E-4	45.000004	39
CGATAGT	20	7.0330506E-4	45.000004	11
ATCGCTC	30	2.1653505E-6	45.000004	28
>>END_MODULE