##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289657
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.377309625737695	33.0	31.0	34.0	31.0	34.0
2	32.518310682607854	34.0	31.0	34.0	31.0	34.0
3	32.571013843215674	34.0	31.0	34.0	31.0	34.0
4	36.02068612041806	37.0	35.0	37.0	35.0	37.0
5	31.651585654170063	37.0	35.0	37.0	0.0	37.0
6	33.41397751495165	37.0	35.0	37.0	17.0	37.0
7	35.26520927657509	37.0	35.0	37.0	32.0	37.0
8	35.81195387610814	37.0	35.0	37.0	35.0	37.0
9	37.84802935974449	39.0	38.0	39.0	35.0	39.0
10	37.567686601941446	39.0	37.0	39.0	35.0	39.0
11	37.49885512194328	39.0	37.0	39.0	35.0	39.0
12	37.3081137077533	39.0	37.0	39.0	35.0	39.0
13	37.245038021737564	39.0	37.0	39.0	34.0	39.0
14	38.48493048926963	40.0	38.0	41.0	34.0	41.0
15	38.558195706300204	40.0	38.0	41.0	35.0	41.0
16	38.601221875273815	40.0	38.0	41.0	35.0	41.0
17	38.594443328730044	40.0	38.0	41.0	35.0	41.0
18	38.58159960361554	40.0	38.0	41.0	35.0	41.0
19	38.60090085968595	40.0	38.0	41.0	35.0	41.0
20	38.53928990421484	40.0	38.0	41.0	34.0	41.0
21	38.487065165388934	40.0	38.0	41.0	34.0	41.0
22	38.45125176694268	40.0	38.0	41.0	34.0	41.0
23	38.39747932977528	40.0	37.0	41.0	34.0	41.0
24	38.390822521026905	40.0	37.0	41.0	34.0	41.0
25	38.32618362867026	40.0	37.0	41.0	34.0	41.0
26	38.296675782785655	40.0	37.0	41.0	34.0	41.0
27	38.226620721633736	40.0	37.0	41.0	34.0	41.0
28	38.231110287464034	40.0	37.0	41.0	34.0	41.0
29	38.18987529242271	40.0	37.0	41.0	34.0	41.0
30	38.130247034676664	40.0	37.0	41.0	34.0	41.0
31	37.960432890295635	40.0	37.0	41.0	34.0	41.0
32	37.99241116048686	40.0	37.0	41.0	34.0	41.0
33	37.982338714867595	40.0	37.0	41.0	34.0	41.0
34	37.90271211647748	40.0	37.0	41.0	34.0	41.0
35	37.841236856001245	40.0	36.0	41.0	33.0	41.0
36	37.81525475378337	40.0	36.0	41.0	33.0	41.0
37	37.77552325928522	40.0	36.0	41.0	33.0	41.0
38	37.80804430945592	40.0	36.0	41.0	33.0	41.0
39	37.80339268503176	40.0	36.0	41.0	33.0	41.0
40	37.708609343414565	40.0	36.0	41.0	33.0	41.0
41	37.695110405324826	40.0	36.0	41.0	33.0	41.0
42	37.67671559182015	40.0	36.0	41.0	33.0	41.0
43	37.58271617957333	40.0	36.0	41.0	33.0	41.0
44	37.527650375254815	39.0	35.0	41.0	33.0	41.0
45	37.546257648351464	39.0	35.0	41.0	33.0	41.0
46	37.49723608680448	39.0	35.0	41.0	33.0	41.0
47	37.48310674853857	39.0	35.0	41.0	33.0	41.0
48	37.44262389146882	39.0	35.0	41.0	33.0	41.0
49	37.41393254175335	39.0	35.0	41.0	33.0	41.0
50	37.34277020944329	39.0	35.0	41.0	33.0	41.0
51	36.7108704097291	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	6.0
12	12.0
13	17.0
14	14.0
15	35.0
16	40.0
17	74.0
18	121.0
19	219.0
20	346.0
21	603.0
22	970.0
23	1510.0
24	2266.0
25	3470.0
26	4751.0
27	6291.0
28	8306.0
29	10603.0
30	14238.0
31	19463.0
32	27129.0
33	41191.0
34	69132.0
35	103496.0
36	102221.0
37	164168.0
38	275747.0
39	432657.0
40	546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.68144630704133	5.155556865119951	55.31447508911285	14.848521738725879
2	19.568924140294666	4.69116982267378	52.99463345680285	22.745272580228697
3	20.247321574651245	4.961009012473859	52.43409681799114	22.357572594883756
4	19.044366060122965	5.010324450609736	51.359935238594446	24.585374250672853
5	15.932220737762057	17.76565396845828	44.25820198703996	22.0439233067397
6	21.11809574173598	7.231147506662625	53.76685428761291	17.883902463988488
7	83.13427523752439	2.626977560700248	9.111569975582654	5.127177226192701
8	84.79045203492092	3.0285572055205376	6.929904618049605	5.251086141508944
9	79.09800823009529	5.687868944998554	9.5717698581871	5.642352966719058
10	36.869570746330226	25.889441921379095	20.344556730975754	16.896430601314925
11	28.315048109691183	25.747000946763364	24.650585388207872	21.28736555533758
12	23.397228875584748	24.157508546846177	31.15355478239563	21.291707795173444
13	22.276233137958386	25.526864895084504	32.617277307066914	19.57962465989019
14	20.831507912569	26.408417121761836	31.43882443161244	21.32125053405673
15	18.51988551994833	25.62611609133281	35.01628727638434	20.83771111233452
16	20.001907483927898	25.529811414973132	31.310650816457397	23.157630284641577
17	19.98795028445548	25.301145963616683	29.22482489530162	25.48607885662622
18	21.86216955360999	24.380591118413655	31.857075175802557	21.900164152173794
19	22.373933534265312	25.932321539758245	30.21935289770846	21.47439202826798
20	24.276532442347072	26.508753877969106	29.01019418341466	20.204519496269164
21	23.523851690798406	27.717369812283422	28.419959725725523	20.338818771192653
22	22.52816058843553	25.35883572143601	28.34373790860671	23.769265781521753
23	21.16632561991289	25.530741894937957	28.171909275101832	25.13102321004732
24	22.029733487276072	24.908483418459326	29.93516880845062	23.12661428581398
25	20.006559883752036	26.784331027552287	28.873878868567378	24.3352302201283
26	19.446643564916872	27.187616552308096	29.768457814752296	23.597282068022736
27	19.82294517069267	27.96573042289539	28.851469809414443	23.359854596997494
28	17.657097972561697	26.373756743071997	31.32825239579206	24.640892888574246
29	19.253801592206297	24.945469997061235	32.0910133469597	23.70971506377277
30	20.378364169697836	27.71031367255014	29.745118275634532	22.166203882117493
31	22.256693058697003	28.80075865133132	26.201617949578843	22.740930340392833
32	22.444262311606884	27.66409983429703	27.563220298110274	22.328417555985816
33	22.748451720108527	27.95285878338194	26.395080242265966	22.90360925424357
34	21.464079208657804	26.148968291569	28.55480178062849	23.832150719144703
35	20.60501358113049	26.555277876210496	29.523276344020154	23.31643219863886
36	23.23935744155229	27.375263345215046	27.369990625414353	22.01538858781831
37	21.19928011866721	27.703102452822726	28.492382082987955	22.605235345522104
38	20.58865264174893	28.045208919891103	28.162216775468206	23.203921662891762
39	22.34733731527065	26.39787168216045	28.95638142544878	22.29840957712012
40	24.20155126518136	25.517947795421573	28.150508235910788	22.129992703486277
41	22.76791425937284	25.300680723634265	27.071073936713407	24.860331080279487
42	23.037055589199298	24.70974840597151	27.941925643795212	24.31127036103398
43	22.59779150580348	24.645080048415974	28.481681563392435	24.275446882388106
44	20.159468757972082	25.469872997238802	28.90993496720446	25.460723277584663
45	20.443730387226992	25.586803312818834	27.911917664929515	26.057548635024663
46	22.26049251855338	25.70226036845456	29.348191030638375	22.68905608235368
47	20.320829491872644	26.012187736739307	30.3352751933266	23.331707578061454
48	20.98317614683594	24.668186967542532	30.02565798503013	24.322978900591398
49	21.28596983539034	23.694051984364837	31.57576006643627	23.444218113808553
50	22.049428646531595	23.10048330680173	29.383781889293044	25.466306157373626
51	20.99930446622629	23.642798046302232	27.877334826236744	27.480562661234732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	275.0
1	493.5
2	712.0
3	1248.5
4	1785.0
5	1406.5
6	1028.0
7	1115.0
8	1202.0
9	1445.5
10	1689.0
11	1704.0
12	1719.0
13	1785.0
14	1851.0
15	1884.5
16	1918.0
17	1971.5
18	2025.0
19	2318.5
20	2612.0
21	3174.5
22	3737.0
23	4381.0
24	5025.0
25	6884.0
26	10880.0
27	13017.0
28	14626.5
29	16236.0
30	18856.5
31	21477.0
32	25681.0
33	29885.0
34	34266.0
35	38647.0
36	42105.5
37	45564.0
38	50206.0
39	54848.0
40	62566.5
41	70285.0
42	78363.0
43	86441.0
44	96828.5
45	107216.0
46	121512.0
47	135808.0
48	145742.5
49	155677.0
50	146219.0
51	136761.0
52	113916.5
53	91072.0
54	79976.5
55	68881.0
56	58904.0
57	48927.0
58	44220.0
59	39513.0
60	34708.5
61	29904.0
62	26206.5
63	22509.0
64	19075.5
65	15642.0
66	12936.5
67	10231.0
68	8739.0
69	7247.0
70	5712.0
71	4177.0
72	3208.0
73	2239.0
74	1853.5
75	1203.0
76	938.0
77	697.5
78	457.0
79	312.5
80	168.0
81	116.0
82	64.0
83	47.0
84	30.0
85	16.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1289657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.6284278018286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.58947922466511	17.322120884725237
2	12.573266922207821	6.4446612709784565
3	5.466111306702087	4.202635169417202
4	3.071597690088952	3.148808785468328
5	1.8938262293048798	2.4267894393474707
6	1.357919432307968	2.0880804078962907
7	0.968657841650682	1.737762429159374
8	0.7260605000946688	1.488623128514863
9	0.5699525497585692	1.3146288994760054
>10	4.1706451143387735	22.909314252389123
>50	1.365174726336921	24.710546905138138
>100	0.2373679275113421	8.96949201455299
>500	0.006928251689215273	1.150744484572837
>1k	0.002711055008823368	1.690604687217265
>5k	3.0122833431370753E-4	0.3951872411464076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	5119	0.3969272449961502	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	4840	0.37529358581390243	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	4705	0.36482568620958905	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	4524	0.3507909467401022	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	2029	0.15732865405297689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3627321062887264	0.0
2	0.0	0.0	0.0	1.577396160374425	0.0
3	0.0	0.0	0.0	2.236098435475479	0.0
4	0.0	0.0	0.0	2.9878487070593187	0.0
5	0.0	0.0	0.0	4.261288078923311	0.0
6	0.0	0.0	0.0	5.111669226778903	0.0
7	0.0	0.0	0.0	5.884432837568439	0.0
8	0.0	0.0	0.0	7.085217232178788	0.0
9	0.0	0.0	0.0	7.670178970067235	0.0
10	0.0	0.0	0.0	8.824051666450847	0.0
11	0.0	0.0	0.0	10.755883153427616	0.0
12	0.0	0.0	0.0	12.407407550999995	0.0
13	0.0	0.0	0.0	12.90668759212721	0.0
14	0.0	0.0	0.0	13.097668604908126	0.0
15	0.0	0.0	0.0	13.400229673471319	0.0
16	0.0	0.0	0.0	14.176482584128959	0.0
17	0.0	0.0	0.0	15.31507990108998	0.0
18	0.0	0.0	0.0	16.55029205439896	0.0
19	0.0	0.0	0.0	17.354691983992645	0.0
20	0.0	0.0	0.0	18.139939534310287	0.0
21	0.0	0.0	0.0	19.25294865223854	0.0
22	7.753999706898811E-5	0.0	0.0	20.357195750498	0.0
23	1.5507999413797623E-4	0.0	0.0	21.474236948273845	0.0
24	1.5507999413797623E-4	0.0	0.0	22.296936317175806	0.0
25	1.5507999413797623E-4	0.0	0.0	23.077066227686895	0.0
26	1.5507999413797623E-4	0.0	0.0	23.738404862688295	0.0
27	1.5507999413797623E-4	0.0	0.0	24.406877177420043	0.0
28	1.5507999413797623E-4	0.0	0.0	25.076279972116616	0.0
29	1.5507999413797623E-4	0.0	0.0	25.777629245605613	0.0
30	1.5507999413797623E-4	0.0	0.0	26.543336716661873	0.0
31	1.5507999413797623E-4	0.0	0.0	27.289504108456743	0.0
32	1.5507999413797623E-4	0.0	0.0	27.962473743018492	0.0
33	1.5507999413797623E-4	0.0	0.0	28.64777223711421	0.0
34	1.5507999413797623E-4	0.0	0.0	29.30748253217716	0.0
35	1.5507999413797623E-4	0.0	0.0	30.018912005285127	0.0
36	1.5507999413797623E-4	0.0	0.0	30.700333499527392	0.0
37	1.5507999413797623E-4	0.0	0.0	31.36647961434707	0.0
38	1.5507999413797623E-4	0.0	0.0	32.08046790735831	0.0
39	1.5507999413797623E-4	0.0	0.0	32.73777446251213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.0336065E-4	45.000004	45
ATCTCCG	20	7.0336065E-4	45.000004	1
GCGACGC	20	7.0336065E-4	45.000004	33
CGAAAGA	20	7.0336065E-4	45.000004	42
CTATCGA	60	0.0	45.000004	42
CGGCACG	35	1.2120836E-7	45.000004	10
CAACCGA	20	7.0336065E-4	45.000004	25
CGCGACA	20	7.0336065E-4	45.000004	18
CTAACCG	20	7.0336065E-4	45.000004	26
CGGATAG	30	2.1657088E-6	45.000004	33
CGCTAGA	20	7.0336065E-4	45.000004	12
GTGTCGT	30	2.1657088E-6	45.000004	15
CTACCCG	20	7.0336065E-4	45.000004	29
CGTCGTT	20	7.0336065E-4	45.000004	30
CGTAACG	20	7.0336065E-4	45.000004	16
TCAAGCG	30	2.1657088E-6	45.000004	17
TATCGCC	30	2.1657088E-6	45.000004	25
TCGAATA	20	7.0336065E-4	45.000004	13
CCGTTCA	20	7.0336065E-4	45.000004	27
TCGAAAC	30	2.1657088E-6	45.000004	34
>>END_MODULE