##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186207
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43329789825891	33.0	31.0	34.0	31.0	34.0
2	32.58394951302766	34.0	31.0	34.0	31.0	34.0
3	32.644192792657606	34.0	31.0	34.0	31.0	34.0
4	36.04286014161104	37.0	35.0	37.0	35.0	37.0
5	32.127981035350494	37.0	35.0	37.0	0.0	37.0
6	33.63976692095056	37.0	35.0	37.0	17.0	37.0
7	35.29352802672721	37.0	35.0	37.0	32.0	37.0
8	35.8209014109679	37.0	35.0	37.0	35.0	37.0
9	37.852100855921435	39.0	38.0	39.0	35.0	39.0
10	37.64718468193157	39.0	37.0	39.0	35.0	39.0
11	37.586266983755785	39.0	37.0	39.0	35.0	39.0
12	37.3682342120726	39.0	37.0	39.0	35.0	39.0
13	37.288627532968526	39.0	37.0	39.0	34.0	39.0
14	38.536172860217484	40.0	38.0	41.0	35.0	41.0
15	38.601277854539724	40.0	38.0	41.0	35.0	41.0
16	38.6418786940222	40.0	38.0	41.0	35.0	41.0
17	38.63077692173457	40.0	38.0	41.0	35.0	41.0
18	38.59616829103184	40.0	38.0	41.0	35.0	41.0
19	38.60805576092537	40.0	38.0	41.0	35.0	41.0
20	38.52531050651362	40.0	38.0	41.0	34.0	41.0
21	38.46942059859704	40.0	38.0	41.0	34.0	41.0
22	38.4414592056867	40.0	37.0	41.0	34.0	41.0
23	38.4001257790588	40.0	37.0	41.0	34.0	41.0
24	38.37981650757414	40.0	37.0	41.0	34.0	41.0
25	38.30554363614445	40.0	37.0	41.0	34.0	41.0
26	38.27693227236056	40.0	37.0	41.0	34.0	41.0
27	38.17332893837248	40.0	37.0	41.0	34.0	41.0
28	38.13329039535258	40.0	37.0	41.0	34.0	41.0
29	38.09023888747917	40.0	37.0	41.0	34.0	41.0
30	38.036036711973544	40.0	36.0	41.0	34.0	41.0
31	37.840226874398816	40.0	36.0	41.0	34.0	41.0
32	37.846069868075304	40.0	36.0	41.0	34.0	41.0
33	37.83605138057691	40.0	36.0	41.0	34.0	41.0
34	37.73948560411463	40.0	36.0	41.0	33.0	41.0
35	37.66502305246892	40.0	36.0	41.0	33.0	41.0
36	37.657882646114885	40.0	36.0	41.0	33.0	41.0
37	37.61882116696327	40.0	35.0	41.0	33.0	41.0
38	37.605530063471214	40.0	35.0	41.0	33.0	41.0
39	37.57082785719525	39.0	35.0	41.0	33.0	41.0
40	37.41959455643071	39.0	35.0	41.0	33.0	41.0
41	37.35459241093671	39.0	35.0	41.0	33.0	41.0
42	37.37135086877754	39.0	35.0	41.0	33.0	41.0
43	37.3271435761212	39.0	35.0	41.0	33.0	41.0
44	37.27110192403181	39.0	35.0	41.0	33.0	41.0
45	37.23071268336808	39.0	35.0	41.0	33.0	41.0
46	37.12301394276041	39.0	35.0	41.0	33.0	41.0
47	37.058306855380216	39.0	35.0	41.0	33.0	41.0
48	37.03310046222961	39.0	35.0	41.0	33.0	41.0
49	36.99125026239096	39.0	35.0	41.0	33.0	41.0
50	36.893249660472414	39.0	35.0	41.0	33.0	41.0
51	36.19399143657051	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	13.0
10	11.0
11	16.0
12	15.0
13	15.0
14	31.0
15	32.0
16	54.0
17	77.0
18	142.0
19	234.0
20	384.0
21	600.0
22	1009.0
23	1523.0
24	2355.0
25	3406.0
26	4769.0
27	6319.0
28	7845.0
29	10080.0
30	13268.0
31	17868.0
32	24667.0
33	38370.0
34	68132.0
35	101055.0
36	98382.0
37	158714.0
38	254500.0
39	371892.0
40	425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.958977648926368	4.4299182183210855	55.24996901889805	17.361135113854495
2	18.793262895936376	5.616304742764122	54.193070855255456	21.397361506044057
3	21.652628925642826	4.504441467635918	51.23043448571792	22.61249512100333
4	20.5441377432438	4.905298990816949	49.44212940911662	25.108433856822625
5	16.253824163910682	17.035138049261217	43.297670642645	23.4133671441831
6	21.43782661879419	7.729763860776408	51.87172222048934	18.96068729994006
7	81.94800738825518	3.73164211642656	8.885042829792775	5.435307665525494
8	83.94892291143114	3.4339706307583753	7.152124376268223	5.464982081542261
9	78.07996412093337	5.62726404413395	9.742144499231586	6.550627335701105
10	40.19441800630075	27.559945270935003	17.075940371284272	15.169696351479969
11	30.586651402326915	25.304773955979016	23.080288684858544	21.02828595683553
12	26.976910438060138	21.105506880333703	29.909450879989745	22.00813180161641
13	24.028183951030467	22.82856196262541	31.305075758278278	21.838178328065844
14	21.007210377278167	24.746776911618294	30.030846218240153	24.21516649286339
15	19.433791909843727	23.264067738598744	34.337598749628015	22.96454160192951
16	21.826881817423097	23.91968686746917	31.150296702009005	23.103134613098725
17	21.43462313070147	23.643681077585953	28.64955273405063	26.272143057661946
18	23.732451418681563	22.546486405829675	29.802218331201892	23.918843844286876
19	25.924564599601922	24.532143209406115	27.47075341824825	22.072538772743712
20	26.734709877786926	24.769201328267325	28.227366724357555	20.26872206958819
21	26.121157605712998	24.83276527621233	28.16540452045891	20.880672597615764
22	24.538634487909782	23.232032857671552	27.840334781366156	24.388997873052514
23	23.716771187490885	25.118297228055475	26.91663428052608	24.24829730392756
24	24.344233342072673	22.641495118474264	28.50615449074234	24.508117048710723
25	23.70690781625804	23.695105491705917	27.737485953126225	24.86050073890982
26	22.32089340224767	25.513169286642214	26.879204051232204	25.286733259877913
27	21.410934179278996	26.11938725703018	28.38678240812944	24.082896155561382
28	20.993722006361455	24.326951366835637	30.28914852129519	24.390178105507722
29	24.11838743153598	23.629433985805175	28.29017195143849	23.96200663122035
30	23.945146167574464	24.46554437800485	27.411573190851175	24.177736263569514
31	23.63668398517291	27.067619732475023	25.297945468202432	23.997750814149637
32	24.779992025000695	27.545782481472457	25.468404755662377	22.20582073786447
33	25.38325941425063	25.203611174103674	25.000611191807167	24.412518219838528
34	22.69072767232026	24.30182927600326	28.597622506021292	24.409820545655183
35	22.959146253562825	24.233713002873866	28.741779470193652	24.065361273369657
36	23.446582257565503	25.358558835009404	27.82330571308381	23.371553194341292
37	22.738189877483443	26.796250570094426	27.144840655973194	23.320718896448934
38	23.923058960198347	27.205116813507253	25.455422198655043	23.416402027639357
39	26.14391923163495	23.81237001636308	26.951113928681924	23.092596823320044
40	24.317593809512168	22.97575381025403	29.49046835838939	23.216184021844416
41	20.079716272117768	25.843887281056343	28.365538223935623	25.71085822289027
42	22.84230324049681	25.24222163585276	26.687837788851354	25.22763733479907
43	23.858989198343966	23.67141654028344	27.51770980950205	24.951884451870544
44	23.47111423217027	23.15607646894682	27.59476212836377	25.77804717051914
45	23.93022465724785	23.032826479695366	26.907108118566153	26.129840744490636
46	23.683893283381398	24.827707137118562	27.895215590533525	23.593183988966512
47	20.38075985051513	25.327619884219192	30.315029332991628	23.976590932274046
48	21.736931243872277	23.76827990392908	29.35153813794725	25.14325071425139
49	22.676143371266566	22.226221898876	30.642628141631267	24.45500658822617
50	23.158605538493703	22.21197480709522	28.993000378517408	25.63641927589367
51	21.93200680825522	23.56460550308673	26.681768021938833	27.821619666719215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	709.0
2	1088.0
3	1709.5
4	2331.0
5	1829.5
6	1328.0
7	1429.5
8	1531.0
9	1602.5
10	1674.0
11	1783.5
12	1893.0
13	1917.5
14	1942.0
15	2066.5
16	2191.0
17	2082.0
18	1973.0
19	1963.5
20	1954.0
21	2511.5
22	3069.0
23	4153.0
24	5237.0
25	5493.0
26	7279.0
27	8809.0
28	10674.5
29	12540.0
30	14317.0
31	16094.0
32	19054.5
33	22015.0
34	24612.5
35	27210.0
36	28155.0
37	29100.0
38	33266.5
39	37433.0
40	41389.0
41	45345.0
42	53928.0
43	62511.0
44	69829.5
45	77148.0
46	91690.5
47	106233.0
48	121920.0
49	137607.0
50	129043.0
51	120479.0
52	104621.5
53	88764.0
54	78296.0
55	67828.0
56	65494.0
57	63160.0
58	59980.0
59	56800.0
60	53898.0
61	50996.0
62	45500.0
63	40004.0
64	34809.0
65	29614.0
66	25007.5
67	20401.0
68	17107.5
69	13814.0
70	11209.0
71	8604.0
72	6685.5
73	4767.0
74	4026.0
75	2465.0
76	1645.0
77	1251.5
78	858.0
79	710.5
80	563.0
81	399.0
82	235.0
83	128.0
84	21.0
85	27.5
86	34.0
87	17.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1186207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.571350960079062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20631383717782	16.759212749865927
2	12.391560818471765	6.089547796276686
3	5.1880906082500235	3.824351854440041
4	2.84947058446631	2.8006136712537324
5	1.8203524392694632	2.2364259328162994
6	1.2670088760081053	1.8679271857158273
7	0.9241597153822577	1.5895496896876964
8	0.7032375911487494	1.3823598128349208
9	0.5554985013412936	1.2284413770829385
>10	4.2579973059917515	24.224180377571056
>50	1.6347636196628152	27.617283227285565
>100	0.1929330215121667	7.181538925686308
>500	0.0062014185485924495	1.068333701605862
>1k	0.002067139516197483	1.6765728177737003
>5k	3.445232526995805E-4	0.4536608801034719
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	5359	0.45177612339161716	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4976	0.4194883355097382	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	4887	0.41198542918731723	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	3542	0.29859881116870834	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	3344	0.2819069521592774	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1577	0.13294475584784105	No Hit
TCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTG	1479	0.12468312866135504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46863658703750694	0.0
2	0.0	0.0	0.0	1.5341335871395128	0.0
3	0.0	0.0	0.0	2.112110280920615	0.0
4	0.0	0.0	0.0	2.7323224361346714	0.0
5	0.0	0.0	0.0	3.8058281564684746	0.0
6	0.0	0.0	0.0	4.540691464474581	0.0
7	0.0	0.0	0.0	5.2183134984028925	0.0
8	0.0	0.0	0.0	6.2746215458178884	0.0
9	0.0	0.0	0.0	6.845854054140635	0.0
10	0.0	0.0	0.0	7.767025485433824	0.0
11	0.0	0.0	0.0	9.385967204712163	0.0
12	0.0	0.0	0.0	10.83520835739462	0.0
13	0.0	0.0	0.0	11.31109494379986	0.0
14	0.0	0.0	0.0	11.490574579310357	0.0
15	0.0	0.0	0.0	11.775094903334747	0.0
16	0.0	0.0	0.0	12.443443682257818	0.0
17	0.0	0.0	0.0	13.355931974773375	0.0
18	0.0	0.0	0.0	14.381048164443474	0.0
19	0.0	0.0	0.0	15.053864966232707	0.0
20	0.0	0.0	0.0	15.785271879191406	0.0
21	0.0	0.0	0.0	16.758710747786854	0.0
22	0.0	0.0	0.0	17.63874264778407	0.0
23	0.0	0.0	0.0	18.492303619857243	0.0
24	0.0	0.0	0.0	19.189905303205933	0.0
25	0.0	0.0	0.0	19.817283155469493	0.0
26	0.0	0.0	0.0	20.381434269060964	0.0
27	0.0	0.0	0.0	20.938166778648245	0.0
28	0.0	0.0	0.0	21.492033009415724	0.0
29	8.4302318229449E-5	0.0	0.0	22.1111492344928	0.0
30	8.4302318229449E-5	0.0	0.0	22.74569278380586	0.0
31	8.4302318229449E-5	0.0	0.0	23.357474707196975	0.0
32	8.4302318229449E-5	0.0	0.0	23.948012446394262	0.0
33	8.4302318229449E-5	0.0	0.0	24.535515302135295	0.0
34	8.4302318229449E-5	0.0	0.0	25.08213153353504	0.0
35	8.4302318229449E-5	0.0	0.0	25.656820436905196	0.0
36	8.4302318229449E-5	0.0	0.0	26.23564015386859	0.0
37	8.4302318229449E-5	0.0	0.0	26.8213726609268	0.0
38	8.4302318229449E-5	0.0	0.0	27.406430749439178	0.0
39	8.4302318229449E-5	0.0	0.0	27.988791163768212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	25	3.8908816E-5	45.0	9
GCGAAAA	25	3.8908816E-5	45.0	13
TAGGTAC	20	7.033379E-4	45.0	23
CGCGACT	25	3.8908816E-5	45.0	13
CTAACGA	20	7.033379E-4	45.0	41
TACGATC	40	6.8157533E-9	45.0	11
TATTCCG	20	7.033379E-4	45.0	14
GTCACGA	20	7.033379E-4	45.0	2
TGTTACG	40	6.8157533E-9	45.0	1
CTCGACG	25	3.8908816E-5	45.0	23
CGTCGGT	20	7.033379E-4	45.0	26
GCGCTTA	20	7.033379E-4	45.0	10
TCGCGAA	25	3.8908816E-5	45.0	12
ACGACCG	40	6.8157533E-9	45.0	18
TACCGTT	20	7.033379E-4	45.0	39
GTACGCC	20	7.033379E-4	45.0	9
ACGAACG	90	0.0	45.0	21
CGAATAT	130	0.0	43.26923	14
TCCGATA	120	0.0	43.125004	14
CGATAAC	95	0.0	42.63158	16
>>END_MODULE