SRR2935326.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283730
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	5670	0.4416816620317356	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	5392	0.4200260179321197	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	4999	0.38941210379129565	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	4855	0.37819479173969606	TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	2320	0.18072336083132745	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1851	0.14418919866327032	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	1627	0.12674004658300422	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	1343	0.10461701448123827	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1343	0.10461701448123827	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.0335943E-4	45.000004	12
GTCGATT	20	7.0335943E-4	45.000004	31
ACGTTAC	20	7.0335943E-4	45.000004	21
CGTTGCA	20	7.0335943E-4	45.000004	31
TAGCGCA	40	6.8157533E-9	45.000004	24
ATAGCGG	20	7.0335943E-4	45.000004	2
TACGGTA	20	7.0335943E-4	45.000004	4
TCTACGA	20	7.0335943E-4	45.000004	42
CAATCGA	20	7.0335943E-4	45.000004	41
AGTCGAT	20	7.0335943E-4	45.000004	30
CTAGCGT	20	7.0335943E-4	45.000004	30
TCGTTAG	45	3.8562575E-10	45.0	1
GGTACGA	25	3.8910614E-5	45.0	16
CTATCCG	25	3.8910614E-5	45.0	1
CGCGAAT	25	3.8910614E-5	45.0	21
ATGCGTC	35	1.2120836E-7	45.0	12
CGCGTTA	25	3.8910614E-5	45.0	35
CGTCCCA	45	3.8562575E-10	45.0	37
TATCGAG	25	3.8910614E-5	45.0	23
CGATAAC	50	2.1827873E-11	45.0	16