##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283730
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32500525811503	33.0	31.0	34.0	30.0	34.0
2	32.47806080717908	34.0	31.0	34.0	31.0	34.0
3	32.54244662039525	34.0	31.0	34.0	31.0	34.0
4	35.973933771120095	37.0	35.0	37.0	35.0	37.0
5	31.678727614062147	37.0	35.0	37.0	0.0	37.0
6	33.413451426701876	37.0	35.0	37.0	17.0	37.0
7	35.20475567292188	37.0	35.0	37.0	32.0	37.0
8	35.75188474211867	37.0	35.0	37.0	35.0	37.0
9	37.75607954943796	39.0	38.0	39.0	35.0	39.0
10	37.543409439679685	39.0	37.0	39.0	35.0	39.0
11	37.488722706488126	39.0	37.0	39.0	35.0	39.0
12	37.31003404142616	39.0	37.0	39.0	35.0	39.0
13	37.223242426366916	39.0	37.0	39.0	34.0	39.0
14	38.45106759209491	40.0	38.0	41.0	34.0	41.0
15	38.54335491107943	40.0	38.0	41.0	34.0	41.0
16	38.598060339791076	40.0	38.0	41.0	35.0	41.0
17	38.591436672820606	40.0	38.0	41.0	35.0	41.0
18	38.56474725993784	40.0	38.0	41.0	35.0	41.0
19	38.5763486091312	40.0	38.0	41.0	34.0	41.0
20	38.49680696096531	40.0	38.0	41.0	34.0	41.0
21	38.44786676326019	40.0	38.0	41.0	34.0	41.0
22	38.413322895001286	40.0	38.0	41.0	34.0	41.0
23	38.368722394896125	40.0	37.0	41.0	34.0	41.0
24	38.334236171157485	40.0	37.0	41.0	34.0	41.0
25	38.26786240097217	40.0	37.0	41.0	34.0	41.0
26	38.238155219555516	40.0	37.0	41.0	34.0	41.0
27	38.183400715103645	40.0	37.0	41.0	34.0	41.0
28	38.169093189377826	40.0	37.0	41.0	34.0	41.0
29	38.126305375741005	40.0	37.0	41.0	34.0	41.0
30	38.080949265032366	40.0	37.0	41.0	34.0	41.0
31	37.88594408481534	40.0	37.0	41.0	34.0	41.0
32	37.937418304472125	40.0	37.0	41.0	34.0	41.0
33	37.940529550606435	40.0	37.0	41.0	34.0	41.0
34	37.91564815031198	40.0	37.0	41.0	34.0	41.0
35	37.82669720268281	40.0	36.0	41.0	33.0	41.0
36	37.80171453498789	40.0	36.0	41.0	33.0	41.0
37	37.7722387106323	40.0	36.0	41.0	33.0	41.0
38	37.75776292522571	40.0	36.0	41.0	33.0	41.0
39	37.75251104204155	40.0	36.0	41.0	33.0	41.0
40	37.66598428018353	40.0	36.0	41.0	33.0	41.0
41	37.61293963683952	40.0	36.0	41.0	33.0	41.0
42	37.57056078770458	40.0	36.0	41.0	33.0	41.0
43	37.50905953744167	40.0	35.0	41.0	33.0	41.0
44	37.46430713623581	39.0	35.0	41.0	33.0	41.0
45	37.45626338871881	39.0	35.0	41.0	33.0	41.0
46	37.375395137606816	39.0	35.0	41.0	33.0	41.0
47	37.33068090642113	39.0	35.0	41.0	33.0	41.0
48	37.31190047751474	39.0	35.0	41.0	33.0	41.0
49	37.28496412797084	39.0	35.0	41.0	33.0	41.0
50	37.21242706799716	39.0	35.0	41.0	33.0	41.0
51	36.57471353010368	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	17.0
11	19.0
12	13.0
13	19.0
14	26.0
15	26.0
16	62.0
17	89.0
18	165.0
19	234.0
20	415.0
21	731.0
22	1207.0
23	1719.0
24	2642.0
25	3828.0
26	5349.0
27	6749.0
28	8654.0
29	11071.0
30	14506.0
31	19750.0
32	27497.0
33	41520.0
34	68982.0
35	103294.0
36	103526.0
37	164249.0
38	268282.0
39	428543.0
40	537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.756085781277996	5.332975002531684	54.38277519416077	16.528164022029554
2	19.58083086007182	4.980174958908805	52.58932953191091	22.84966464910846
3	21.083872776985814	4.907028736572332	50.95206936037952	23.057029126062332
4	19.19546945229916	5.114626907527284	48.98389848332593	26.706005156847624
5	16.619226784448443	17.47711746239474	43.03700933997025	22.866646413186572
6	21.202355635530836	6.801040717284787	52.71085041246991	19.285753234714463
7	81.44119090462948	3.3129240572394503	9.471773659570157	5.774111378560912
8	82.48494621143075	3.589306162510808	7.697023517406308	6.228724108652131
9	76.23090525266217	6.30576523100652	10.597867152750188	6.865462363581126
10	35.86026656695723	28.571818061430363	17.892080110303567	17.67583526130884
11	27.9036090143566	25.159651951734396	25.83681926884937	21.09991976505963
12	25.147967251680647	21.34857018220342	32.35906304285169	21.144399523264237
13	22.552483777741426	25.54361119550061	31.684933747750694	20.218971279007267
14	18.38478496257001	27.731766025566124	31.311646530033578	22.571802481830293
15	16.970936256066306	24.03028674253932	36.70951056686375	22.289266434530624
16	18.79935812047705	24.06853466071526	33.71994110911173	23.412166109695963
17	19.949444197767445	23.555264736354218	28.581711107475872	27.91357995840247
18	20.429140083974044	23.522158086201927	32.17935235602502	23.869349473799005
19	22.32354155468829	24.79431033005383	29.495922039681243	23.38622607557664
20	24.615144929229665	25.4350213830011	28.94767591315931	21.002157774609927
21	23.628800448692484	25.873898717019934	28.79647589446379	21.700824939823796
22	22.786255676816776	24.659079401431764	27.910464038388135	24.644200883363325
23	21.21333925358136	25.672610284094006	27.40864512007977	25.705405342244863
24	22.75034469865159	23.39892344963505	29.413428057301772	24.437303794411598
25	21.012440310657226	25.463843643133682	27.640859059147953	25.882856987061142
26	19.512825905758998	26.81436127534606	27.57706059685448	26.09575222204046
27	19.929112819673918	25.884337049067952	30.0803907363698	24.106159394888333
28	18.520794871195655	24.942783918736804	31.78028090018929	24.756140309878248
29	19.06522399570003	23.027194191925094	30.611499302812895	27.29608250956198
30	20.22348936302805	24.476486488591835	31.004416816620317	24.295607331759793
31	20.836858217849546	25.73578556238461	28.000046738800215	25.427309480965626
32	21.4774134747961	25.158639277729737	27.465900150343142	25.898047097131016
33	21.3198258200712	24.376699150132815	28.528662569231848	25.77481246056414
34	19.725876936739034	23.17722574061524	30.262048873205426	26.8348484494403
35	19.903484377556026	22.778855366782736	29.91929767162877	27.398362584032466
36	22.27173938445	23.54303475029796	29.997351467987816	24.187874397264224
37	20.56522789060005	24.582271973078452	31.140037235244172	23.71246290107733
38	20.03700155017021	24.564277534995675	29.383671021164886	26.01504989366923
39	21.805286158304316	22.69643928240362	29.49265032366619	26.005624235625874
40	21.162705553348445	23.06185880208455	29.90434125555997	25.871094389007034
41	19.564705973997647	23.309418647223325	29.02611919952015	28.09975617925888
42	21.282278983898482	22.683741908345212	30.203781168937393	25.830197938818912
43	22.316297040654966	22.54516136570774	29.663402740451655	25.475138853185637
44	20.349917817609622	22.805963870907433	29.62048094225421	27.223637369228733
45	19.612067958215512	22.843199115078715	28.518535829185264	29.026197097520505
46	21.042197346794108	22.8355651110436	30.861318189962063	25.26091935220023
47	18.527416201226117	23.29321586314879	32.991672703761694	25.1876952318634
48	19.406728829270953	21.457705280705444	32.346521464793994	26.7890444252296
49	20.9780872924992	20.10500650448303	33.32305079728604	25.593855405731738
50	20.124636800573327	20.66088663504008	31.041262570789808	28.173213993596786
51	19.27103051264674	21.470714246765287	29.013188131460666	30.245067109127312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	377.0
1	664.0
2	951.0
3	1531.0
4	2111.0
5	1620.0
6	1129.0
7	1241.0
8	1353.0
9	1721.5
10	2090.0
11	2118.5
12	2147.0
13	2103.5
14	2060.0
15	1994.5
16	1929.0
17	2200.5
18	2472.0
19	2626.5
20	2781.0
21	2925.0
22	3069.0
23	3539.5
24	4010.0
25	5690.5
26	9463.0
27	11555.0
28	12938.5
29	14322.0
30	16446.5
31	18571.0
32	23193.0
33	27815.0
34	31983.0
35	36151.0
36	37995.0
37	39839.0
38	44735.5
39	49632.0
40	55186.0
41	60740.0
42	69140.0
43	77540.0
44	85126.5
45	92713.0
46	108283.5
47	123854.0
48	137142.0
49	150430.0
50	142535.0
51	134640.0
52	114629.0
53	94618.0
54	81532.5
55	68447.0
56	62842.5
57	57238.0
58	53707.0
59	50176.0
60	46623.5
61	43071.0
62	38303.5
63	33536.0
64	28619.5
65	23703.0
66	18896.5
67	14090.0
68	12216.5
69	10343.0
70	8913.0
71	7483.0
72	5886.5
73	4290.0
74	3412.5
75	2018.5
76	1502.0
77	1091.0
78	680.0
79	484.0
80	288.0
81	176.0
82	64.0
83	37.0
84	10.0
85	5.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1283730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.17891343701313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.0279207177671	16.206622928978696
2	12.554064261740372	6.070872661346412
3	5.534640604076251	4.0146478821281315
4	3.1529060118215635	3.0493536613948753
5	1.982485271792304	2.3967169888409785
6	1.353113219408147	1.9630084441528646
7	1.038244837663223	1.757254243939937
8	0.7524482176773073	1.4554704256843543
9	0.5889020462362804	1.2815110438944235
>10	4.218809488907245	21.41915205420273
>50	1.4749659210842854	25.67693351345265
>100	0.3095681301438596	10.784511850555875
>500	0.008061670055459505	1.361961949993926
>1k	0.003224668022183802	1.6994896166714908
>5k	6.449336044367604E-4	0.8624927347625835
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	5670	0.4416816620317356	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	5392	0.4200260179321197	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	4999	0.38941210379129565	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	4855	0.37819479173969606	TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	2320	0.18072336083132745	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1851	0.14418919866327032	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	1627	0.12674004658300422	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	1343	0.10461701448123827	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1343	0.10461701448123827	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.557960007166616E-4	0.0	0.0	0.38902261378950403	0.0
2	1.557960007166616E-4	0.0	0.0	1.7125875378779027	0.0
3	1.557960007166616E-4	0.0	0.0	2.4611873213214617	0.0
4	1.557960007166616E-4	0.0	0.0	3.2638483170137023	0.0
5	1.557960007166616E-4	0.0	0.0	4.685253129552164	0.0
6	1.557960007166616E-4	0.0	0.0	5.63046746590015	0.0
7	1.557960007166616E-4	0.0	0.0	6.468805745756506	0.0
8	1.557960007166616E-4	0.0	0.0	7.80475645190188	0.0
9	1.557960007166616E-4	0.0	0.0	8.441105216829085	0.0
10	1.557960007166616E-4	0.0	0.0	9.644785118366011	0.0
11	1.557960007166616E-4	0.0	0.0	11.65112601559518	0.0
12	1.557960007166616E-4	0.0	0.0	13.356235345438682	0.0
13	1.557960007166616E-4	0.0	0.0	13.905961533967423	0.0
14	1.557960007166616E-4	0.0	0.0	14.118623074945667	0.0
15	1.557960007166616E-4	0.0	0.0	14.458024662506913	0.0
16	1.557960007166616E-4	0.0	0.0	15.285301426312387	0.0
17	1.557960007166616E-4	0.0	0.0	16.470831093765824	0.0
18	1.557960007166616E-4	0.0	0.0	17.747423523638147	0.0
19	1.557960007166616E-4	0.0	0.0	18.525702445218233	0.0
20	1.557960007166616E-4	0.0	0.0	19.34526730698823	0.0
21	1.557960007166616E-4	0.0	0.0	20.441915356032812	0.0
22	1.557960007166616E-4	0.0	0.0	21.44570898865026	0.0
23	1.557960007166616E-4	0.0	0.0	22.446931987255887	0.0
24	1.557960007166616E-4	0.0	0.0	23.221783396820204	0.0
25	1.557960007166616E-4	0.0	0.0	23.96025644021718	0.0
26	1.557960007166616E-4	0.0	0.0	24.583752035085258	0.0
27	1.557960007166616E-4	0.0	0.0	25.19727668590747	0.0
28	1.557960007166616E-4	0.0	0.0	25.818746932766235	0.0
29	1.557960007166616E-4	0.0	0.0	26.46872784775615	0.0
30	1.557960007166616E-4	0.0	0.0	27.19847631511299	0.0
31	2.336940010749924E-4	0.0	0.0	27.912255692396375	0.0
32	3.89490001791654E-4	0.0	0.0	28.573531817438248	0.0
33	3.89490001791654E-4	0.0	0.0	29.223201140426724	0.0
34	3.89490001791654E-4	0.0	0.0	29.861575253363245	0.0
35	3.89490001791654E-4	0.0	0.0	30.51186776035459	0.0
36	3.89490001791654E-4	0.0	0.0	31.15125454729577	0.0
37	3.89490001791654E-4	0.0	0.0	31.790563436236592	0.0
38	3.89490001791654E-4	0.0	0.0	32.432520857189594	0.0
39	3.89490001791654E-4	0.0	0.0	33.066922172107844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.0335943E-4	45.000004	12
GTCGATT	20	7.0335943E-4	45.000004	31
ACGTTAC	20	7.0335943E-4	45.000004	21
CGTTGCA	20	7.0335943E-4	45.000004	31
TAGCGCA	40	6.8157533E-9	45.000004	24
ATAGCGG	20	7.0335943E-4	45.000004	2
TACGGTA	20	7.0335943E-4	45.000004	4
TCTACGA	20	7.0335943E-4	45.000004	42
CAATCGA	20	7.0335943E-4	45.000004	41
AGTCGAT	20	7.0335943E-4	45.000004	30
CTAGCGT	20	7.0335943E-4	45.000004	30
TCGTTAG	45	3.8562575E-10	45.0	1
GGTACGA	25	3.8910614E-5	45.0	16
CTATCCG	25	3.8910614E-5	45.0	1
CGCGAAT	25	3.8910614E-5	45.0	21
ATGCGTC	35	1.2120836E-7	45.0	12
CGCGTTA	25	3.8910614E-5	45.0	35
CGTCCCA	45	3.8562575E-10	45.0	37
TATCGAG	25	3.8910614E-5	45.0	23
CGATAAC	50	2.1827873E-11	45.0	16
>>END_MODULE