##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3581273
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46647267605681	34.0	31.0	34.0	31.0	34.0
2	32.6264817007807	34.0	31.0	34.0	31.0	34.0
3	32.67970607099766	34.0	31.0	34.0	31.0	34.0
4	36.08216435887462	37.0	35.0	37.0	35.0	37.0
5	31.84363911938576	37.0	35.0	37.0	0.0	37.0
6	33.461889668841216	37.0	35.0	37.0	17.0	37.0
7	35.26777238149675	37.0	35.0	37.0	32.0	37.0
8	35.81612208843057	37.0	35.0	37.0	35.0	37.0
9	37.883789926096114	39.0	38.0	39.0	35.0	39.0
10	37.673222622235166	39.0	37.0	39.0	35.0	39.0
11	37.57831949700567	39.0	37.0	39.0	35.0	39.0
12	37.36623904404942	39.0	37.0	39.0	35.0	39.0
13	37.301905774845984	39.0	37.0	39.0	35.0	39.0
14	38.46505390680912	40.0	38.0	41.0	34.0	41.0
15	38.58581487644198	40.0	38.0	41.0	34.0	41.0
16	38.66817413807883	40.0	38.0	41.0	35.0	41.0
17	38.682704725386756	40.0	38.0	41.0	35.0	41.0
18	38.625511933884965	40.0	38.0	41.0	35.0	41.0
19	38.631529905706714	40.0	38.0	41.0	35.0	41.0
20	38.552812924342824	40.0	38.0	41.0	34.0	41.0
21	38.49094218731719	40.0	38.0	41.0	34.0	41.0
22	38.487819833897056	40.0	38.0	41.0	34.0	41.0
23	38.42503014989363	40.0	37.0	41.0	34.0	41.0
24	38.40991820506284	40.0	37.0	41.0	34.0	41.0
25	38.31892486275132	40.0	37.0	41.0	34.0	41.0
26	38.261224988991344	40.0	37.0	41.0	34.0	41.0
27	38.226036384268944	40.0	37.0	41.0	34.0	41.0
28	38.16831612669573	40.0	37.0	41.0	34.0	41.0
29	38.10917737910514	40.0	37.0	41.0	34.0	41.0
30	38.056519008743535	40.0	37.0	41.0	34.0	41.0
31	37.84028193326786	40.0	36.0	41.0	33.0	41.0
32	37.85060926659319	40.0	36.0	41.0	33.0	41.0
33	37.83606025008426	40.0	36.0	41.0	33.0	41.0
34	37.72059683805172	40.0	36.0	41.0	33.0	41.0
35	37.57630289564632	40.0	36.0	41.0	33.0	41.0
36	37.525330797177425	40.0	36.0	41.0	33.0	41.0
37	37.46842421675198	40.0	35.0	41.0	33.0	41.0
38	37.45903202576291	40.0	35.0	41.0	33.0	41.0
39	37.442107038474866	40.0	35.0	41.0	33.0	41.0
40	37.352271385063354	39.0	35.0	41.0	33.0	41.0
41	37.25292710162001	39.0	35.0	41.0	33.0	41.0
42	37.229603272355945	39.0	35.0	41.0	33.0	41.0
43	37.16936714961412	39.0	35.0	41.0	33.0	41.0
44	37.10901989320557	39.0	35.0	41.0	33.0	41.0
45	37.05588292207827	39.0	35.0	41.0	33.0	41.0
46	36.98333860613251	39.0	35.0	41.0	33.0	41.0
47	36.93169663412982	39.0	35.0	41.0	33.0	41.0
48	36.87987455857177	39.0	35.0	41.0	33.0	41.0
49	36.82767021670786	39.0	35.0	41.0	32.0	41.0
50	36.7489993083465	38.0	35.0	41.0	32.0	41.0
51	36.13696107501439	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	20.0
10	32.0
11	34.0
12	45.0
13	63.0
14	75.0
15	115.0
16	183.0
17	302.0
18	493.0
19	859.0
20	1455.0
21	2368.0
22	3837.0
23	5882.0
24	8835.0
25	12788.0
26	17656.0
27	21770.0
28	26542.0
29	32471.0
30	41713.0
31	55369.0
32	76798.0
33	120675.0
34	211668.0
35	269160.0
36	294873.0
37	477052.0
38	749315.0
39	1147369.0
40	1455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.986673733055255	4.331895390270443	54.49296939942864	16.18846147724566
2	19.591916058898608	4.381430848751268	51.71585634493656	24.31079674741356
3	20.311799742717184	4.5763056879495085	53.02131392943236	22.09058063990095
4	18.54737128389821	4.904876003588668	49.00109542053901	27.546657291974107
5	16.173857731594325	18.936618347721605	42.27826250609769	22.611261414586377
6	21.0221895957108	8.879300740267498	51.158596398543196	18.939913265478506
7	82.62246413495983	2.591285277609386	7.913247607764055	6.873002979666727
8	84.93463078631537	2.7995352490580863	8.121246271926212	4.144587692700333
9	79.67532773960544	5.2576555878314775	10.094343547671457	4.972673124891624
10	42.45029630525235	28.773790772163977	14.53243022802227	14.243482694561402
11	29.697568434464504	26.32424280416489	24.52114094625012	19.45704781512049
12	26.5932253698615	21.20606276036482	28.957217168308585	23.243494701465092
13	25.845418654204806	20.692306897575246	29.867731390486007	23.59454305773394
14	22.230893874887506	22.491248223746137	30.958991397751586	24.318866503614775
15	20.910413699262804	21.824055301006094	34.315982054425895	22.949548945305203
16	26.304501220655336	22.041715334184243	29.93737143189028	21.71641201327014
17	25.101772470291987	22.34401007686373	27.613728414449273	24.940489038395004
18	24.8315612911945	23.785341134283815	29.332139716799027	22.050957857722658
19	24.910248394914323	25.11054030228916	28.319594736285115	21.659616566511406
20	26.636896991656318	26.237206713925467	28.027547746290217	19.098348548127998
21	26.786871595658862	26.647200590404584	27.052950166044308	19.512977647892242
22	27.400563989397064	23.015335608315816	27.425108334382774	22.158992067904347
23	25.061004843808334	24.673907853436475	28.01727207057379	22.247815232181406
24	24.446642297306013	25.9301650558335	27.084503192021387	22.538689454839105
25	24.50000321114866	26.265157668795425	24.865348159718625	24.369490960337288
26	23.16427706014035	25.714375865788508	26.884797668315148	24.236549405755998
27	23.095418863627543	23.708832027047364	28.380048100214644	24.815701009110448
28	23.180975033179543	24.63774752720611	27.350190839961098	24.83108659965325
29	22.27833510598047	26.262728365025513	26.886528896289114	24.572407632704905
30	23.96781814734593	25.321024116284907	27.25377261102407	23.45738512534509
31	25.306169063347028	25.457176819527582	25.830368140044058	23.406285977081335
32	25.237673866248116	26.629246080932674	24.513490035526473	23.619590017292733
33	25.23962847847679	24.848063803010827	25.398203376285473	24.51410434222691
34	23.579827619955253	25.72833738170757	27.567515796757185	23.124319201579997
35	24.23160702912065	27.923869529075272	25.594390597980105	22.25013284382397
36	24.90703724625294	27.949642487461862	25.037828727382696	22.10549153890251
37	24.630990153501283	28.770691315629943	24.374098260590575	22.224220270278195
38	24.46495980619182	26.993920876738525	24.446642297306013	24.094477019763644
39	23.843811962952838	25.24610662186323	26.278839954396105	24.631241460787827
40	23.659799183139626	24.322440651690055	28.339029166444448	23.67873099872587
41	22.422948487870094	26.752358728306945	26.270211737558125	24.554481046264833
42	23.029492585457742	24.607841960107482	26.58683099557057	25.77583445886421
43	23.4216436445923	24.180256573570347	27.95324455856898	24.444855223268373
44	24.735841138053424	23.51420849513567	27.12529874153688	24.62465162527403
45	22.964794920688817	24.385127858166637	26.28023610598801	26.36984111515654
46	22.880411518474016	24.23057387694264	27.909154091296585	24.979860513286756
47	22.086336339061557	25.333645326675736	28.989049424604048	23.59096890965866
48	22.297378613694068	24.84792418785164	28.080657352846323	24.774039845607973
49	23.624504470896245	24.23886701739856	27.954864094415587	24.181764417289607
50	21.788146282062275	24.288681706197767	28.335399172305493	25.587772839434468
51	21.896822721976235	24.22462627116112	26.74172005317662	27.13683095368602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	573.0
1	1664.0
2	2755.0
3	7436.0
4	12117.0
5	8356.5
6	4596.0
7	4660.0
8	4724.0
9	5163.5
10	5603.0
11	5424.0
12	5245.0
13	5251.5
14	5258.0
15	5395.0
16	5532.0
17	5385.0
18	5238.0
19	5931.5
20	6625.0
21	7945.5
22	9266.0
23	10443.0
24	11620.0
25	15786.0
26	24469.5
27	28987.0
28	33854.5
29	38722.0
30	56375.0
31	74028.0
32	72999.5
33	71971.0
34	80497.0
35	89023.0
36	95705.0
37	102387.0
38	116957.5
39	131528.0
40	140406.0
41	149284.0
42	160089.5
43	170895.0
44	185413.0
45	199931.0
46	233102.5
47	266274.0
48	294405.5
49	322537.0
50	320715.0
51	318893.0
52	289102.5
53	259312.0
54	242390.5
55	225469.0
56	215599.5
57	205730.0
58	195728.5
59	185727.0
60	176170.0
61	166613.0
62	158116.0
63	149619.0
64	136924.5
65	124230.0
66	104708.5
67	85187.0
68	65713.0
69	46239.0
70	36980.0
71	27721.0
72	23077.0
73	18433.0
74	14933.5
75	8827.5
76	6221.0
77	4761.0
78	3301.0
79	2345.5
80	1390.0
81	1057.5
82	725.0
83	476.5
84	228.0
85	137.0
86	46.0
87	50.5
88	55.0
89	40.0
90	25.0
91	14.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3581273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.067073297247777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39652586312837	15.534452960932526
2	12.107853272446553	5.343697712707988
3	4.7977937145807905	3.17619796694187
4	2.3179207077018993	2.0459890461626498
5	1.4018770621591978	1.5467661942198698
6	0.844453337980355	1.1180768223190827
7	0.6044094352356946	0.9336283116225943
8	0.45255149547957896	0.7989189617221572
9	0.3533544469561888	0.7017748632801603
>10	4.423696969189062	24.92208522547449
>50	2.041505396517512	31.382748706558576
>100	0.24754178841762428	7.855798135588193
>500	0.006146012458714012	0.9138982085025232
>1k	0.0032778733113141296	1.6035940368628976
>5k	8.194683278285324E-4	1.1645624369797651
>10k+	2.731561092761775E-4	0.957810410124662
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	20858	0.5824185980795097	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	10922	0.3049753537359481	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATG	7240	0.20216275050798974	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA	7057	0.19705283568161377	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	6949	0.19403714824309679	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	6307	0.17611056180302367	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCAGG	5569	0.155503364306491	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5518	0.1540792896827469	No Hit
TCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	4980	0.13905669855383826	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	4818	0.1345331673960628	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	4467	0.12473218322088264	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3997	0.11160835825696618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3961515919060067E-4	0.0	0.0	0.18161139907513335	0.0
2	1.3961515919060067E-4	0.0	0.0	0.6799258252582252	0.0
3	1.3961515919060067E-4	0.0	0.0	1.0056479916498966	0.0
4	1.3961515919060067E-4	0.0	0.0	1.3672791769853905	0.0
5	1.3961515919060067E-4	0.0	0.0	2.0893129342555006	0.0
6	1.3961515919060067E-4	0.0	0.0	2.714928462588582	0.0
7	1.3961515919060067E-4	0.0	0.0	3.2386807707762015	0.0
8	1.3961515919060067E-4	0.0	0.0	4.1539977544297795	0.0
9	1.675381910287208E-4	0.0	0.0	4.628940602964365	0.0
10	1.675381910287208E-4	0.0	0.0	5.347037212745301	0.0
11	1.675381910287208E-4	0.0	0.0	6.546638583542779	0.0
12	1.675381910287208E-4	0.0	0.0	7.540866055170885	0.0
13	1.675381910287208E-4	0.0	0.0	8.104073607345768	0.0
14	1.675381910287208E-4	0.0	0.0	8.246620684879371	0.0
15	1.675381910287208E-4	0.0	0.0	8.655972331626213	0.0
16	1.675381910287208E-4	0.0	0.0	9.080514107692991	0.0
17	1.954612228668409E-4	0.0	0.0	9.667651698153144	0.0
18	1.954612228668409E-4	0.0	0.0	10.273078874467263	0.0
19	1.954612228668409E-4	0.0	0.0	10.724426761098638	0.0
20	1.954612228668409E-4	0.0	0.0	11.248681683859342	0.0
21	2.2338425470496106E-4	0.0	0.0	11.888984726939276	0.0
22	2.2338425470496106E-4	0.0	0.0	12.57309900697322	0.0
23	2.2338425470496106E-4	0.0	0.0	13.26304920066133	0.0
24	2.2338425470496106E-4	0.0	0.0	13.807017783899747	0.0
25	2.2338425470496106E-4	0.0	0.0	14.443383679490506	0.0
26	2.2338425470496106E-4	0.0	0.0	14.879150514356208	0.0
27	2.2338425470496106E-4	0.0	0.0	15.353814132572412	0.0
28	2.2338425470496106E-4	0.0	0.0	15.79203819423987	0.0
29	2.2338425470496106E-4	0.0	0.0	16.280914635661677	0.0
30	2.513072865430812E-4	0.0	0.0	16.820834379283568	0.0
31	2.513072865430812E-4	0.0	0.0	17.28527816784702	0.0
32	2.513072865430812E-4	0.0	0.0	17.841895884508105	0.0
33	2.513072865430812E-4	0.0	0.0	18.298214070806665	0.0
34	2.513072865430812E-4	0.0	0.0	18.87784036570236	0.0
35	2.513072865430812E-4	0.0	0.0	19.382353704953516	0.0
36	2.513072865430812E-4	0.0	0.0	19.861987622836907	0.0
37	2.513072865430812E-4	0.0	0.0	20.353488829251496	0.0
38	2.7923031838120133E-4	0.0	0.0	20.896731413662124	0.0
39	2.7923031838120133E-4	0.0	0.0	21.662911484268303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAT	20	7.035287E-4	45.000004	32
CGACGTA	25	3.8924656E-5	45.0	33
TACGGGA	1635	0.0	41.972477	4
TTGACCC	6835	0.0	41.839798	8
CTTGACC	7720	0.0	41.823185	7
CTCAACT	7505	0.0	41.822117	2
TGCATTA	5795	0.0	41.738567	15
ACTTGAC	7380	0.0	41.615856	6
GCATTAG	5825	0.0	41.600857	16
TTACGGG	2715	0.0	41.187847	3
TCAACTT	7455	0.0	41.016098	3
TATGGGA	4645	0.0	40.97955	4
AACTTGA	7500	0.0	40.829998	5
CATTAGA	5905	0.0	40.656223	17
CCTGCAT	6240	0.0	40.492786	13
TTTACGG	1640	0.0	40.47256	2
TTATGGG	6935	0.0	40.392933	3
ACCCTGC	6320	0.0	40.300632	11
GCTCAAC	8230	0.0	40.29769	1
TTTTACG	965	0.0	40.103626	1
>>END_MODULE