FastQCFastQC Report
Sat 14 Jan 2017
SRR2935324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977942
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC155021.585165582416953No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG140501.4366905194786603No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC129091.3200169335195748No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC58830.6015694182272568No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC51490.526513842334208No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT48510.4960416875438421No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC41970.4291665558898176No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT40010.4091244675042078No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC34290.35063429119518336No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT31960.32680874734902476No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT28720.293677948180976No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT27290.2790554041037199No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA24540.2509351270320735No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC22100.22598477210304907No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG21130.21606598346323194No Hit
GAATATGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC16580.1695397068537807No Hit
TCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG14920.15256528505780506No Hit
TGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG10430.10665254176628063No Hit
TGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCTT9980.1020510418818294Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT302.1651686E-645.00000428
CATATGG302.1651686E-645.0000042
GATCGAA302.1651686E-645.00000442
GCGTTCG302.1651686E-645.0000041
GCCGATA302.1651686E-645.0000049
GTCACTA302.1651686E-645.00000410
GCTTACG600.045.0000041
TTGCGAA302.1651686E-645.00000416
TAGGCGA302.1651686E-645.00000420
TATACGA302.1651686E-645.00000435
CTTCGTT207.03277E-445.039
TTCTACG900.045.01
CTATGCG253.890378E-545.038
CTCACGA253.890378E-545.024
ACACGTT253.890378E-545.012
CGAACTA253.890378E-545.040
TCGCACC207.03277E-445.011
CTCCGTA207.03277E-445.013
TACTTGA351.2116834E-745.016
CGACGGT406.8139343E-945.028
Produced by FastQC (version 0.11.5)