##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935324.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977942
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.135946712586225	33.0	31.0	34.0	30.0	34.0
2	32.266097580429104	34.0	31.0	34.0	30.0	34.0
3	32.353964754555996	34.0	31.0	34.0	30.0	34.0
4	35.87468172959132	37.0	35.0	37.0	35.0	37.0
5	31.56893353593567	37.0	35.0	37.0	0.0	37.0
6	33.10689897764898	37.0	35.0	37.0	17.0	37.0
7	35.013353552664675	37.0	35.0	37.0	32.0	37.0
8	35.482716766434	37.0	35.0	37.0	33.0	37.0
9	37.58414507199814	39.0	37.0	39.0	35.0	39.0
10	37.38634704307617	39.0	37.0	39.0	34.0	39.0
11	37.35528282863401	39.0	37.0	39.0	35.0	39.0
12	37.104421325600086	39.0	37.0	39.0	34.0	39.0
13	36.9390055851983	39.0	37.0	39.0	33.0	39.0
14	38.1008178399128	40.0	37.0	41.0	33.0	41.0
15	38.119084771898535	40.0	37.0	41.0	33.0	41.0
16	38.29600323945592	40.0	37.0	41.0	34.0	41.0
17	38.18481055113698	40.0	37.0	41.0	34.0	41.0
18	38.20770352433989	40.0	37.0	41.0	34.0	41.0
19	38.261995087643236	40.0	37.0	41.0	34.0	41.0
20	38.0479414934628	40.0	37.0	41.0	33.0	41.0
21	38.036876420073995	40.0	37.0	41.0	34.0	41.0
22	38.032379220853585	40.0	37.0	41.0	34.0	41.0
23	37.88666505784597	40.0	36.0	41.0	33.0	41.0
24	37.926402588292554	40.0	36.0	41.0	33.0	41.0
25	37.84337312437752	40.0	36.0	41.0	33.0	41.0
26	37.813713901233406	40.0	36.0	41.0	33.0	41.0
27	37.62566491673331	40.0	36.0	41.0	33.0	41.0
28	37.59176106558467	40.0	36.0	41.0	33.0	41.0
29	37.51252323757441	40.0	36.0	41.0	33.0	41.0
30	37.492829840624495	40.0	36.0	41.0	33.0	41.0
31	37.40756302521008	40.0	36.0	41.0	32.0	41.0
32	37.274737152101046	39.0	35.0	41.0	32.0	41.0
33	37.244897959183675	39.0	35.0	41.0	32.0	41.0
34	37.1583284080242	39.0	35.0	41.0	32.0	41.0
35	37.0173619703418	39.0	35.0	41.0	31.0	41.0
36	37.04152598006835	39.0	35.0	41.0	31.0	41.0
37	36.90685950700553	39.0	35.0	41.0	31.0	41.0
38	37.012533463129714	39.0	35.0	41.0	31.0	41.0
39	37.003040057590326	39.0	35.0	41.0	32.0	41.0
40	36.9178069865084	39.0	35.0	41.0	31.0	41.0
41	36.84299784649805	39.0	35.0	41.0	31.0	41.0
42	36.7310413091983	39.0	35.0	41.0	31.0	41.0
43	36.69106961353536	39.0	35.0	41.0	31.0	41.0
44	36.656012319749024	39.0	35.0	41.0	31.0	41.0
45	36.72777117661374	39.0	35.0	41.0	31.0	41.0
46	36.635269780825446	39.0	35.0	41.0	31.0	41.0
47	36.46500303698993	39.0	35.0	40.0	31.0	41.0
48	36.48496332093314	39.0	35.0	40.0	31.0	41.0
49	36.47911737096883	39.0	35.0	40.0	31.0	41.0
50	36.3495534500001	39.0	35.0	40.0	31.0	41.0
51	35.69301553670872	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	29.0
11	30.0
12	34.0
13	12.0
14	17.0
15	43.0
16	39.0
17	65.0
18	179.0
19	453.0
20	908.0
21	1755.0
22	2808.0
23	3737.0
24	4699.0
25	5456.0
26	6331.0
27	7196.0
28	8540.0
29	10864.0
30	14199.0
31	19388.0
32	26053.0
33	37991.0
34	59542.0
35	87512.0
36	82102.0
37	125392.0
38	198935.0
39	273296.0
40	326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.23590151563181	3.88714259127842	50.69983700464854	14.177118888441237
2	17.34806358659307	14.431223937615933	47.838113098731824	20.382599377059172
3	18.278589118782097	14.304222540805078	47.010967930613475	20.406220409799356
4	16.8380128882899	4.205259616623481	55.6817275462144	23.274999948872225
5	21.34697149728716	16.379192222033616	39.889175431671816	22.384660849007407
6	18.12653511148923	13.243627945215565	49.62533565385268	19.004501289442523
7	74.69471604655492	2.131721513136771	17.816700785936177	5.356861654372141
8	75.13738033543912	9.682476056862267	8.458272576492266	6.72187103120635
9	70.29752275697332	4.549963085745371	10.863936716083366	14.288577441197944
10	38.78215681502584	24.812412188043872	18.577788866824413	17.827642130105875
11	31.93072799818394	23.07110237621454	25.469301860437533	19.528867765163987
12	25.663280644455398	20.432295575811242	29.375566240124666	24.52885753960869
13	24.325164478056983	24.840021187350576	32.737422055704734	18.097392278887707
14	19.21903343961094	29.389575250883997	27.74142024782656	23.649971061678503
15	14.86826417108581	24.220659302903442	39.070210707792484	21.84086581821826
16	16.512124440917763	24.083125584134844	31.156755717619244	28.247994257328145
17	16.83474071059429	25.57094388010741	29.905454515707476	27.688860893590828
18	18.13665841123502	23.987209875432285	31.269032314799855	26.607099398532835
19	21.22487836701972	24.715882946023385	27.70450599319796	26.354732693758937
20	24.03762186305527	24.739708489869543	31.584388440214244	19.63828120686094
21	21.813461330017528	30.568172754621443	27.302948436614848	20.315417478746184
22	20.080945495745148	24.131594716251065	29.835204950804854	25.952254837198936
23	20.358058044342098	27.058046387209057	28.04317638469357	24.540719183755275
24	21.99690779207765	23.344840491562895	28.448721907843204	26.20952980851625
25	18.951226146335877	31.0296520652554	24.809958054772164	25.20916373363655
26	19.624169940548622	26.35708457147765	28.246869446245277	25.771876041728447
27	21.34564217509832	28.878092974839	25.1568088905068	24.619455959555882
28	16.456190653433435	27.06121630935168	31.780514590844856	24.70207844637003
29	21.395236118297404	23.827997979430275	29.50931650343272	25.267449398839602
30	20.100271795259843	30.073051367054486	28.366712954346983	21.459963883338684
31	23.605592151681797	26.52529495614259	23.047992621239295	26.821120270936312
32	23.96870162034149	31.583161373578395	23.14237449664704	21.305762509433073
33	23.05975200983289	25.86339476165253	25.408050784197833	25.66880244431674
34	25.694059565904727	24.672322080450577	25.184213378707533	24.449404974937163
35	21.721635843434477	28.43675800814363	25.1610013681793	24.68060478024259
36	25.949084915056314	26.641764030995702	24.664243891764542	22.74490716218344
37	20.922713207940756	29.850543283753023	27.440073133171495	21.786670375134722
38	19.320266437068863	27.157847806925155	25.93558718206192	27.586298573944056
39	23.96594072041082	26.72264817340906	28.017919263105583	21.293491843074538
40	22.952383679195698	24.203787136660456	30.494855523129182	22.34897366101466
41	23.99948054179082	25.32798468620838	25.478913882418386	25.19362088958241
42	22.71136734080344	26.252988418536066	26.9897396778132	24.045904562847287
43	25.291070431579787	23.95295426518137	25.847033873174485	24.90894143006436
44	21.439308261635148	23.766542392084602	28.390436242640156	26.403713103640094
45	20.307543801166123	23.928106165805335	26.166173454049424	29.598176578979125
46	25.25108851036156	27.145781651672596	25.867791750431007	21.735338087534846
47	18.57277834472801	24.85065576486131	33.482967292538824	23.093598597871857
48	20.968830462338257	24.570373294121737	28.4638557296854	25.996940513854604
49	21.090616825946736	21.701798266154842	33.41322900540114	23.794355902497287
50	23.207511283900274	21.89301615024204	28.7766554662751	26.12281709958259
51	20.498250407488378	22.414008192714903	26.966834433943937	30.12090696585278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	133.0
1	362.5
2	592.0
3	1029.0
4	1466.0
5	1109.5
6	753.0
7	803.0
8	853.0
9	1085.5
10	1318.0
11	1352.0
12	1386.0
13	1383.0
14	1380.0
15	1361.5
16	1343.0
17	1433.5
18	1524.0
19	1818.5
20	2113.0
21	2334.0
22	2555.0
23	3352.5
24	4150.0
25	4024.0
26	6404.0
27	8910.0
28	8647.5
29	8385.0
30	9906.0
31	11427.0
32	14846.0
33	18265.0
34	21821.0
35	25377.0
36	26520.0
37	27663.0
38	30029.5
39	32396.0
40	37534.5
41	42673.0
42	48698.0
43	54723.0
44	65728.5
45	76734.0
46	114551.5
47	152369.0
48	136845.0
49	121321.0
50	113070.0
51	104819.0
52	86098.0
53	67377.0
54	60111.0
55	52845.0
56	45386.0
57	37927.0
58	35142.0
59	32357.0
60	28980.0
61	25603.0
62	22229.0
63	18855.0
64	16286.0
65	13717.0
66	10782.0
67	7847.0
68	6653.5
69	5460.0
70	4433.0
71	3406.0
72	2589.0
73	1772.0
74	1375.0
75	883.5
76	789.0
77	527.0
78	265.0
79	215.5
80	166.0
81	91.5
82	17.0
83	18.0
84	19.0
85	17.0
86	15.0
87	7.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	977942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.141153398954152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.95473217097218	16.405056135242845
2	12.484880764095504	6.02798843387363
3	5.518466706329445	3.9966645385356125
4	3.091269226910012	2.9850721841720405
5	1.9243051405641833	2.3227472792377992
6	1.3409725637637089	1.9423574619365114
7	0.9219373271795984	1.5579641307765129
8	0.7268382591868158	1.4037371129006173
9	0.5657556981442536	1.2292195585709547
>10	3.9034067489626483	20.22343043758773
>50	1.3207332059348122	22.23004860614671
>100	0.2283171028924546	8.613227765047638
>500	0.010689002944403305	1.7999145058168742
>1k	0.005558281531089719	3.74113437683981
>5k	8.551202355522644E-4	1.1387003571608991
>10k+	0.0012826803533283967	4.38273711615382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	15502	1.585165582416953	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	14050	1.4366905194786603	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	12909	1.3200169335195748	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	5883	0.6015694182272568	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	5149	0.526513842334208	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	4851	0.4960416875438421	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	4197	0.4291665558898176	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	4001	0.4091244675042078	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	3429	0.35063429119518336	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	3196	0.32680874734902476	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	2872	0.293677948180976	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT	2729	0.2790554041037199	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	2454	0.2509351270320735	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	2210	0.22598477210304907	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	2113	0.21606598346323194	No Hit
GAATATGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	1658	0.1695397068537807	No Hit
TCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	1492	0.15256528505780506	No Hit
TGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	1043	0.10665254176628063	No Hit
TGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCTT	998	0.1020510418818294	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.02255552987805E-4	0.0	0.0	0.42845076701890294	0.0
2	1.02255552987805E-4	0.0	0.0	2.0906147808356734	0.0
3	1.02255552987805E-4	0.0	0.0	2.8125389849295765	0.0
4	1.02255552987805E-4	0.0	0.0	3.8777350804035415	0.0
5	1.02255552987805E-4	0.0	0.0	6.547729824468118	0.0
6	1.02255552987805E-4	0.0	0.0	7.7767393158285465	0.0
7	1.02255552987805E-4	0.0	0.0	8.923330831480804	0.0
8	1.02255552987805E-4	0.0	0.0	10.50369040290733	0.0
9	1.02255552987805E-4	0.0	0.0	11.076219244086051	0.0
10	2.0451110597561E-4	0.0	0.0	13.871988318325627	0.0
11	2.0451110597561E-4	0.0	0.0	15.792245347883616	0.0
12	2.0451110597561E-4	0.0	0.0	19.374870902364353	0.0
13	2.0451110597561E-4	0.0	0.0	20.086058273394535	0.0
14	3.06766658963415E-4	0.0	0.0	20.418184309498926	0.0
15	3.06766658963415E-4	0.0	0.0	21.085913070509292	0.0
16	3.06766658963415E-4	0.0	0.0	21.954676248693687	0.0
17	3.06766658963415E-4	0.0	0.0	23.096359497802528	0.0
18	3.06766658963415E-4	0.0	0.0	24.283853234649907	0.0
19	3.06766658963415E-4	0.0	0.0	25.637409989549482	0.0
20	3.06766658963415E-4	0.0	0.0	26.52447691171869	0.0
21	3.06766658963415E-4	0.0	0.0	27.646936116865827	0.0
22	3.06766658963415E-4	0.0	0.0	28.70436079031272	0.0
23	3.06766658963415E-4	0.0	0.0	29.67374343263711	0.0
24	3.06766658963415E-4	0.0	0.0	30.440353313386684	0.0
25	4.0902221195122E-4	0.0	0.0	31.123318151792233	0.0
26	4.0902221195122E-4	0.0	0.0	31.788490523977906	0.0
27	5.11277764939025E-4	0.0	0.0	32.40468248628242	0.0
28	5.11277764939025E-4	0.0	0.0	33.0076834822515	0.0
29	5.11277764939025E-4	0.0	0.0	33.67357164330809	0.0
30	5.11277764939025E-4	0.0	0.0	34.414515380257725	0.0
31	6.1353331792683E-4	0.0	0.0	35.111080207210655	0.0
32	6.1353331792683E-4	0.0	0.0	35.71735338087535	0.0
33	6.1353331792683E-4	0.0	0.0	36.34796337615114	0.0
34	6.1353331792683E-4	0.0	0.0	36.96395082734968	0.0
35	6.1353331792683E-4	0.0	0.0	37.590777367164925	0.0
36	6.1353331792683E-4	0.0	0.0	38.19950467410133	0.0
37	6.1353331792683E-4	0.0	0.0	38.76917035979639	0.0
38	6.1353331792683E-4	0.0	0.0	39.40346155497974	0.0
39	6.1353331792683E-4	0.0	0.0	40.03325350583163	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	30	2.1651686E-6	45.000004	28
CATATGG	30	2.1651686E-6	45.000004	2
GATCGAA	30	2.1651686E-6	45.000004	42
GCGTTCG	30	2.1651686E-6	45.000004	1
GCCGATA	30	2.1651686E-6	45.000004	9
GTCACTA	30	2.1651686E-6	45.000004	10
GCTTACG	60	0.0	45.000004	1
TTGCGAA	30	2.1651686E-6	45.000004	16
TAGGCGA	30	2.1651686E-6	45.000004	20
TATACGA	30	2.1651686E-6	45.000004	35
CTTCGTT	20	7.03277E-4	45.0	39
TTCTACG	90	0.0	45.0	1
CTATGCG	25	3.890378E-5	45.0	38
CTCACGA	25	3.890378E-5	45.0	24
ACACGTT	25	3.890378E-5	45.0	12
CGAACTA	25	3.890378E-5	45.0	40
TCGCACC	20	7.03277E-4	45.0	11
CTCCGTA	20	7.03277E-4	45.0	13
TACTTGA	35	1.2116834E-7	45.0	16
CGACGGT	40	6.8139343E-9	45.0	28
>>END_MODULE