SRR2935323.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160220
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	3978	0.34286600817086416	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3625	0.3124407439968282	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3311	0.28537691127544773	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3192	0.275120235817345	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	1758	0.15152298701970315	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1629	0.14040440606091947	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	1628	0.14031821551085139	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1445	0.12454534484839083	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	1381	0.11902914964403302	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	1276	0.10997914188688351	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	30	2.1655196E-6	45.000004	20
CGTTAAT	30	2.1655196E-6	45.000004	17
AACGTCG	20	7.033314E-4	45.0	31
CTATGCG	25	3.8908285E-5	45.0	1
TCCGCAT	25	3.8908285E-5	45.0	19
CGACGGC	20	7.033314E-4	45.0	16
CGCATGA	25	3.8908285E-5	45.0	21
ATGTCGA	20	7.033314E-4	45.0	28
CACTACG	20	7.033314E-4	45.0	6
CGCTAAG	20	7.033314E-4	45.0	14
ACGCTAG	20	7.033314E-4	45.0	22
TACTCGA	35	1.211938E-7	45.0	44
ACGTCGT	20	7.033314E-4	45.0	32
CGCACAT	40	6.8157533E-9	45.0	21
TATACGG	40	6.8157533E-9	45.0	2
CCACGTT	20	7.033314E-4	45.0	24
ACCCCGT	20	7.033314E-4	45.0	24
CGTCAAT	45	3.8562575E-10	45.0	32
CGATAAG	25	3.8908285E-5	45.0	28
TCGAATA	20	7.033314E-4	45.0	27