##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160220
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.414257640792265	33.0	31.0	34.0	31.0	34.0
2	32.56103239040871	34.0	31.0	34.0	31.0	34.0
3	32.61852752064264	34.0	31.0	34.0	31.0	34.0
4	36.02812742410922	37.0	35.0	37.0	35.0	37.0
5	31.85639016738205	37.0	35.0	37.0	0.0	37.0
6	33.50305373118891	37.0	35.0	37.0	17.0	37.0
7	35.25944562238196	37.0	35.0	37.0	32.0	37.0
8	35.78669735050249	37.0	35.0	37.0	35.0	37.0
9	37.831194945786145	39.0	38.0	39.0	35.0	39.0
10	37.62166054713761	39.0	37.0	39.0	35.0	39.0
11	37.54996983330748	39.0	37.0	39.0	35.0	39.0
12	37.285740635396735	39.0	37.0	39.0	35.0	39.0
13	37.20972315595318	39.0	37.0	39.0	34.0	39.0
14	38.41319146368792	40.0	38.0	41.0	34.0	41.0
15	38.49906224681526	40.0	38.0	41.0	34.0	41.0
16	38.57201737601489	40.0	38.0	41.0	35.0	41.0
17	38.54844253676027	40.0	38.0	41.0	34.0	41.0
18	38.51887659237041	40.0	38.0	41.0	34.0	41.0
19	38.53616555480857	40.0	38.0	41.0	34.0	41.0
20	38.46963248349451	40.0	37.0	41.0	34.0	41.0
21	38.423549843995104	40.0	37.0	41.0	34.0	41.0
22	38.3801304924928	40.0	37.0	41.0	34.0	41.0
23	38.346413611211666	40.0	37.0	41.0	34.0	41.0
24	38.323905810966885	40.0	37.0	41.0	34.0	41.0
25	38.2399984485701	40.0	37.0	41.0	34.0	41.0
26	38.190434572753446	40.0	37.0	41.0	34.0	41.0
27	38.145209529227216	40.0	37.0	41.0	34.0	41.0
28	38.085536363793075	40.0	37.0	41.0	34.0	41.0
29	38.04750650738653	40.0	36.0	41.0	34.0	41.0
30	38.02311113409526	40.0	36.0	41.0	34.0	41.0
31	37.85853975970075	40.0	36.0	41.0	34.0	41.0
32	37.898734722725	40.0	36.0	41.0	34.0	41.0
33	37.86124528106738	40.0	36.0	41.0	34.0	41.0
34	37.80674613435383	40.0	36.0	41.0	33.0	41.0
35	37.73150609367189	40.0	36.0	41.0	33.0	41.0
36	37.72404888728	40.0	36.0	41.0	33.0	41.0
37	37.69280739859682	40.0	36.0	41.0	33.0	41.0
38	37.69280739859682	40.0	36.0	41.0	33.0	41.0
39	37.66601506610815	40.0	36.0	41.0	33.0	41.0
40	37.58684818396511	40.0	35.0	41.0	33.0	41.0
41	37.51297857302925	40.0	35.0	41.0	33.0	41.0
42	37.48694644119219	40.0	35.0	41.0	33.0	41.0
43	37.4270888279809	39.0	35.0	41.0	33.0	41.0
44	37.40244781162193	39.0	35.0	41.0	33.0	41.0
45	37.37040302701212	39.0	35.0	41.0	33.0	41.0
46	37.27756029028977	39.0	35.0	41.0	33.0	41.0
47	37.21821551085139	39.0	35.0	41.0	33.0	41.0
48	37.18447449621623	39.0	35.0	41.0	33.0	41.0
49	37.13814448983813	39.0	35.0	41.0	33.0	41.0
50	37.06605298995018	39.0	35.0	41.0	33.0	41.0
51	36.488419437692855	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	14.0
10	21.0
11	16.0
12	25.0
13	9.0
14	27.0
15	30.0
16	49.0
17	73.0
18	145.0
19	254.0
20	451.0
21	750.0
22	1248.0
23	1801.0
24	2816.0
25	3866.0
26	5147.0
27	6221.0
28	7534.0
29	9953.0
30	13081.0
31	17705.0
32	24651.0
33	38223.0
34	68019.0
35	97113.0
36	89827.0
37	143666.0
38	234687.0
39	392282.0
40	515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.76092465222113	5.107048663184568	54.15248832118047	16.979538363413834
2	19.01553153712227	5.785454482770509	53.0408887969523	22.15812518315492
3	20.89146885935426	5.3771698470979645	51.037044698419265	22.694316595128512
4	19.024495354329353	5.31554360379928	50.50809329265139	25.151867749219974
5	16.886452569340296	16.93247832307666	42.96512730344245	23.215941804140595
6	20.800451638482357	7.695609453379531	52.241471445070765	19.26246746306735
7	82.48470117736292	2.9823654134560686	9.668424953888055	4.864508455292961
8	84.11715019565254	4.081984451224767	6.597714226612195	5.203151126510489
9	78.60276499284619	5.621175294340729	9.025098688179828	6.750961024633259
10	38.930375273655	28.764286083673785	16.36586164692903	15.939476995742186
11	31.54513799107066	24.844598438227234	23.987691989450276	19.622571581251833
12	28.39909672303529	22.649411318543038	28.532605885090756	20.41888607333092
13	24.493889090000174	24.367102790850012	29.59154298322732	21.547465135922497
14	20.00887762665701	26.412232162865664	29.90208753512265	23.676802675354676
15	19.17894882005137	24.251435072658634	34.22092361793453	22.348692489355468
16	21.408439778662665	23.9992415231594	31.58159659374946	23.01072210442847
17	20.77450828291186	23.727396528244643	29.0494906138491	26.4486045749944
18	22.188205685128683	24.105255899743153	30.19375635655307	23.512782058575098
19	24.291082725689954	26.467307924359172	27.01453172674148	22.22707762320939
20	26.979796935064037	25.548171898433054	27.546155039561466	19.925876126941443
21	25.86474978883315	26.360000689524398	27.051335091620555	20.723914430021892
22	22.977452552102186	24.950009480960507	27.656565134198686	24.415972832738618
23	22.855148161555565	26.067297581493165	27.021857923497265	24.055696333453998
24	23.780145144886315	24.734877867990555	27.943062522625016	23.541914464498113
25	21.88817638034166	27.054955094723415	26.510920342693627	24.545948182241297
26	20.766233990105327	27.50168071572633	27.706297081587977	24.025788212580373
27	22.603902708107082	25.840616434814084	28.080536449983622	23.474944407095204
28	19.910189446829047	26.636499974142836	29.907689920877072	23.54562065815104
29	21.700194790643152	24.055523952353862	29.13033734981297	25.113943907190016
30	22.589767457895917	25.21116684766682	29.484666701142885	22.714398993294374
31	23.222923238696108	26.204685318301703	26.49583699643171	24.076554446570476
32	24.01820344417438	26.19744531209598	26.461446966954544	23.322904276775095
33	23.44719104997328	25.12971677785248	27.702763269035184	23.72032890313906
34	21.563324197135024	24.332281808622504	29.465963351778115	24.63843064246436
35	22.006085052834806	23.59707641654169	29.415714261088414	24.981124269535087
36	23.080450259433555	25.678836772336282	28.132767923324884	23.107945044905275
37	22.52090120839151	26.23226629432349	28.557687335160576	22.689145162124426
38	21.431625036630983	25.332523142162692	26.99375980417507	26.242092017031254
39	21.765182465394496	24.22945648239127	30.086104359518025	23.919256692696216
40	22.689145162124426	23.352726207098655	31.419644550171522	22.538484080605404
41	20.66246056782334	24.46199858647498	29.7717674234197	25.103773422281982
42	22.51926358794022	24.128785919911742	28.224043715846996	25.127906776301046
43	22.08675940769854	23.812121838961573	28.8560790194963	25.245039733843583
44	21.6808881074279	23.33428142938408	28.01891020668494	26.96592025650308
45	21.95799072589681	22.695437072279397	27.26663908569065	28.079933116133148
46	22.16260709175846	24.444501904811155	28.962524348830392	24.43036665459999
47	20.34889934667563	24.672475909741255	31.029459930013275	23.94916481356984
48	20.89060695385358	22.478323076657876	30.74373825653755	25.88733171295099
49	21.29380634707211	22.39092585888883	31.536949888814192	24.77831790522487
50	21.39930358035545	22.516677871438176	30.0826567375153	26.001361810691076
51	20.04921480408888	22.689920877075036	29.053800141352504	28.20706417748358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	662.0
2	1153.0
3	1618.5
4	2084.0
5	1581.0
6	1078.0
7	1197.5
8	1317.0
9	1349.5
10	1382.0
11	1456.5
12	1531.0
13	1728.0
14	1925.0
15	1837.0
16	1749.0
17	1820.0
18	1891.0
19	2051.0
20	2211.0
21	2473.0
22	2735.0
23	3970.5
24	5206.0
25	5390.0
26	7988.0
27	10402.0
28	13391.5
29	16381.0
30	16978.0
31	17575.0
32	21037.0
33	24499.0
34	29417.0
35	34335.0
36	35282.0
37	36229.0
38	40382.0
39	44535.0
40	48380.5
41	52226.0
42	59512.5
43	66799.0
44	74218.0
45	81637.0
46	93226.5
47	104816.0
48	111822.0
49	118828.0
50	113995.5
51	109163.0
52	96175.5
53	83188.0
54	74985.0
55	66782.0
56	61499.5
57	56217.0
58	54267.0
59	52317.0
60	49820.0
61	47323.0
62	42038.5
63	36754.0
64	31364.5
65	25975.0
66	21715.0
67	17455.0
68	14359.0
69	11263.0
70	8352.0
71	5441.0
72	5032.0
73	4623.0
74	3490.5
75	1935.0
76	1512.0
77	1167.5
78	823.0
79	643.5
80	464.0
81	349.5
82	235.0
83	140.5
84	46.0
85	26.5
86	7.0
87	5.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1160220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.44144217433346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.43122116017445	16.041265137452537
2	12.15351746305711	5.697919536500103
3	5.086432840196144	3.576999638912658
4	2.7787750233422788	2.6055397610064057
5	1.7372321544956417	2.0361613546501154
6	1.2036089216834238	1.6928597362892297
7	0.9272588731060672	1.5215399678187436
8	0.7057762047223747	1.3235529672816064
9	0.56807676673092	1.198488481011466
>10	4.341292342254041	22.63314326325196
>50	1.7951325449344124	29.764011210842916
>100	0.2616821848084248	8.858594295767722
>500	0.0059220862191433484	0.9460735768956299
>1k	0.004071434275661053	2.1038510723188955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	3978	0.34286600817086416	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3625	0.3124407439968282	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3311	0.28537691127544773	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3192	0.275120235817345	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	1758	0.15152298701970315	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1629	0.14040440606091947	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	1628	0.14031821551085139	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1445	0.12454534484839083	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	1381	0.11902914964403302	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	1276	0.10997914188688351	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7238110013618107E-4	0.0	0.0	0.35363982692937546	0.0
2	1.7238110013618107E-4	0.0	0.0	1.288117770767613	0.0
3	1.7238110013618107E-4	0.0	0.0	1.8036234507248625	0.0
4	1.7238110013618107E-4	0.0	0.0	2.394201099791419	0.0
5	1.7238110013618107E-4	0.0	0.0	3.4497767664753236	0.0
6	1.7238110013618107E-4	0.0	0.0	4.127579252210787	0.0
7	2.585716502042716E-4	0.0	0.0	4.745565496198997	0.0
8	2.585716502042716E-4	0.0	0.0	5.732274913378498	0.0
9	2.585716502042716E-4	0.0	0.0	6.2057196049025185	0.0
10	2.585716502042716E-4	0.0	0.0	7.220871903604489	0.0
11	2.585716502042716E-4	0.0	0.0	8.732137008498388	0.0
12	2.585716502042716E-4	0.0	0.0	10.22521590732792	0.0
13	2.585716502042716E-4	0.0	0.0	10.652979607315855	0.0
14	2.585716502042716E-4	0.0	0.0	10.822688800399924	0.0
15	2.585716502042716E-4	0.0	0.0	11.151160986709417	0.0
16	2.585716502042716E-4	0.0	0.0	11.764837703194221	0.0
17	2.585716502042716E-4	0.0	0.0	12.627346537725604	0.0
18	2.585716502042716E-4	0.0	0.0	13.581217355329162	0.0
19	2.585716502042716E-4	0.0	0.0	14.243246970402165	0.0
20	2.585716502042716E-4	0.0	0.0	14.898122769819517	0.0
21	2.585716502042716E-4	0.0	0.0	15.760803985451036	0.0
22	4.3095275034045266E-4	0.0	0.0	16.613400906724586	0.0
23	5.171433004085432E-4	0.0	0.0	17.425833031666407	0.0
24	5.171433004085432E-4	0.0	0.0	18.073641205978177	0.0
25	5.171433004085432E-4	0.0	0.0	18.688696971264072	0.0
26	5.171433004085432E-4	0.0	0.0	19.197307407215874	0.0
27	5.171433004085432E-4	0.0	0.0	19.674716864043027	0.0
28	6.033338504766337E-4	0.0	0.0	20.224612573477444	0.0
29	6.033338504766337E-4	0.0	0.0	20.783472100118942	0.0
30	6.033338504766337E-4	0.0	0.0	21.412749306166074	0.0
31	6.033338504766337E-4	0.0	0.0	21.9550602471945	0.0
32	6.033338504766337E-4	0.0	0.0	22.4758235507059	0.0
33	6.033338504766337E-4	0.0	0.0	23.017789729534055	0.0
34	6.033338504766337E-4	0.0	0.0	23.537777318094843	0.0
35	6.033338504766337E-4	0.0	0.0	24.101463515540157	0.0
36	6.895244005447243E-4	0.0	0.0	24.637827308613883	0.0
37	6.895244005447243E-4	0.0	0.0	25.18815397079864	0.0
38	6.895244005447243E-4	0.0	0.0	25.745979210839323	0.0
39	6.895244005447243E-4	0.0	0.0	26.31664684284015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	30	2.1655196E-6	45.000004	20
CGTTAAT	30	2.1655196E-6	45.000004	17
AACGTCG	20	7.033314E-4	45.0	31
CTATGCG	25	3.8908285E-5	45.0	1
TCCGCAT	25	3.8908285E-5	45.0	19
CGACGGC	20	7.033314E-4	45.0	16
CGCATGA	25	3.8908285E-5	45.0	21
ATGTCGA	20	7.033314E-4	45.0	28
CACTACG	20	7.033314E-4	45.0	6
CGCTAAG	20	7.033314E-4	45.0	14
ACGCTAG	20	7.033314E-4	45.0	22
TACTCGA	35	1.211938E-7	45.0	44
ACGTCGT	20	7.033314E-4	45.0	32
CGCACAT	40	6.8157533E-9	45.0	21
TATACGG	40	6.8157533E-9	45.0	2
CCACGTT	20	7.033314E-4	45.0	24
ACCCCGT	20	7.033314E-4	45.0	24
CGTCAAT	45	3.8562575E-10	45.0	32
CGATAAG	25	3.8908285E-5	45.0	28
TCGAATA	20	7.033314E-4	45.0	27
>>END_MODULE