FastQCFastQC Report
Sat 14 Jan 2017
SRR2935322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434896
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC263981.0841530808708053No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG225810.9273907386598853No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC217750.8942887088401312No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC110760.4548859581682339TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC98180.403220507159238TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT90990.3736915252232539TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT86590.35562093822487695No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC75950.3119229733015291TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC56310.23126244406331933No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT55790.22912682923623842No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT51970.21343827416037484No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA44340.182102233524553No Hit
TCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG42590.17491506824110764No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC36360.1493287598320421No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG32680.13421517797885413No Hit
TTCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT28780.1181980667757473No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC26420.1085056610220724No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.1025095116998837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA253.8920953E-545.00000421
CGTGCTA207.0348434E-445.041
AATGCGA207.0348434E-445.025
CTCGGTA207.0348434E-445.04
CCGCGTA207.0348434E-445.040
CGGTAAT207.0348434E-445.06
CGATCGC207.0348434E-445.010
CGATCGA1600.045.041
TAATGCG207.0348434E-445.024
AATACGG9950.043.1909565
TAATACG10000.042.9750024
CCCATCG2050.042.8048840
TTACGGG13800.042.7173923
TACGCTA1650.042.27272838
CGATGAA38750.041.86451719
CTACGGG6700.041.6417923
TACGGGA8350.041.4970054
TACGAAT4350.041.3793112
AGCTACG4200.041.259
TACGGGC4500.041.04
Produced by FastQC (version 0.11.5)