##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2434896
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.172082503729115	33.0	31.0	34.0	30.0	34.0
2	32.306889082737	34.0	31.0	34.0	30.0	34.0
3	32.37694505227328	34.0	31.0	34.0	30.0	34.0
4	35.914332275382606	37.0	35.0	37.0	35.0	37.0
5	31.67997606468613	37.0	35.0	37.0	0.0	37.0
6	33.24912768348217	37.0	35.0	37.0	17.0	37.0
7	35.13088279745829	37.0	35.0	37.0	32.0	37.0
8	35.60220806145314	37.0	35.0	37.0	33.0	37.0
9	37.664762683909295	39.0	37.0	39.0	35.0	39.0
10	37.357754910271325	39.0	37.0	39.0	34.0	39.0
11	37.271240332235955	39.0	37.0	39.0	34.0	39.0
12	37.173419727166994	39.0	37.0	39.0	34.0	39.0
13	37.10321878223957	39.0	37.0	39.0	33.0	39.0
14	38.31619625643149	40.0	38.0	41.0	34.0	41.0
15	38.33097635381552	40.0	38.0	41.0	34.0	41.0
16	38.44517630321788	40.0	38.0	41.0	34.0	41.0
17	38.36680211393012	40.0	38.0	41.0	34.0	41.0
18	38.37179616706422	40.0	38.0	41.0	34.0	41.0
19	38.35608830931588	40.0	38.0	41.0	34.0	41.0
20	38.15520293269199	40.0	37.0	41.0	34.0	41.0
21	38.20195564820838	40.0	37.0	41.0	34.0	41.0
22	38.199153064442996	40.0	37.0	41.0	34.0	41.0
23	37.998972440712045	40.0	37.0	41.0	33.0	41.0
24	38.02282643694022	40.0	37.0	41.0	33.0	41.0
25	37.99906977546474	40.0	37.0	41.0	33.0	41.0
26	37.928918113956406	40.0	37.0	41.0	33.0	41.0
27	37.80079067032021	40.0	37.0	41.0	33.0	41.0
28	37.78529226710299	40.0	37.0	41.0	33.0	41.0
29	37.72854323141522	40.0	36.0	41.0	33.0	41.0
30	37.7271994368548	40.0	36.0	41.0	33.0	41.0
31	37.58955166873657	40.0	36.0	41.0	33.0	41.0
32	37.457712772948	40.0	36.0	41.0	32.0	41.0
33	37.41920476274962	40.0	36.0	41.0	32.0	41.0
34	37.372836047206945	40.0	36.0	41.0	32.0	41.0
35	37.2744495042088	40.0	36.0	41.0	32.0	41.0
36	37.302920124719904	40.0	36.0	41.0	32.0	41.0
37	37.337299005789156	40.0	36.0	41.0	32.0	41.0
38	37.370969027013885	40.0	36.0	41.0	32.0	41.0
39	37.31340188656928	40.0	36.0	41.0	32.0	41.0
40	37.27317799199637	40.0	36.0	41.0	32.0	41.0
41	37.21046730537978	40.0	36.0	41.0	32.0	41.0
42	37.06976314388787	39.0	35.0	41.0	32.0	41.0
43	37.04367168043317	39.0	35.0	41.0	32.0	41.0
44	37.04987482011552	39.0	35.0	41.0	32.0	41.0
45	37.04163340035878	39.0	35.0	41.0	32.0	41.0
46	36.937644975391144	39.0	35.0	41.0	31.0	41.0
47	36.80063460615977	39.0	35.0	41.0	31.0	41.0
48	36.806837335146966	39.0	35.0	41.0	31.0	41.0
49	36.82626485895086	39.0	35.0	41.0	31.0	41.0
50	36.76929527996268	39.0	35.0	41.0	31.0	41.0
51	36.16427272458454	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	15.0
11	9.0
12	23.0
13	17.0
14	24.0
15	34.0
16	74.0
17	126.0
18	254.0
19	664.0
20	1438.0
21	3088.0
22	5663.0
23	8897.0
24	12152.0
25	15182.0
26	17351.0
27	18136.0
28	19638.0
29	23880.0
30	31479.0
31	43508.0
32	58753.0
33	83131.0
34	127961.0
35	178873.0
36	203895.0
37	319748.0
38	520219.0
39	739903.0
40	754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.871910751013598	4.240140030621431	54.47045787581892	12.417491342546047
2	18.211783994059704	11.282370992436638	49.826768781911014	20.679076231592646
3	17.61221013135674	11.51174423876831	49.14119535290213	21.734850276972814
4	16.86207542334457	4.18235522174253	55.365239418849924	23.59032993606298
5	19.30148145957774	16.148451514972304	43.563996162464434	20.986070862985525
6	18.896905658393624	11.401308310498683	51.986902109987454	17.714883921120244
7	76.86205078163503	1.9919125909279083	15.901829071960364	5.244207555476702
8	78.25443057937588	7.598722902333406	8.430627016513231	5.716219501777489
9	73.65768394214783	4.6066443905612395	9.946338570518002	11.789333096772921
10	38.01792766508303	23.629592393268542	20.70330724597683	17.649172695671602
11	28.425813669249116	25.578669479107113	24.953303960415557	21.04221289122821
12	22.693535986752618	21.527900986325495	30.977051997292698	24.801511029629193
13	23.465766094321893	24.190601980536336	34.11570761133124	18.22792431381053
14	19.91682601638838	28.242807906374644	29.707387913077195	22.132978164159784
15	17.833780169666383	24.931537116985695	35.1500433694088	22.084639343939124
16	18.030092455694206	24.580023130351357	30.444133958904203	26.945750455050238
17	18.429452428358335	27.198902951091135	30.008879229338746	24.36276539121178
18	19.946354998324363	24.15823920200288	31.073893915797633	24.82151188387512
19	20.86006958818775	27.70237414657546	27.92788685841202	23.509669406824766
20	24.366749134254604	26.43796696039584	29.921606508039766	19.273677397309783
21	22.459604024155446	30.45637267464401	27.74865949100085	19.335363810199695
22	20.502641591263036	24.62573350155407	29.383719058226713	25.487905848956178
23	20.649711527720278	28.42063891024504	27.07138210420486	23.85826745782982
24	21.520220986851186	26.0491207838035	27.733504839631756	24.697153389713563
25	18.92265624486631	31.083709530099025	25.786932994263413	24.20670123077125
26	18.37404965140195	27.701347408677822	29.51382728461503	24.410775655305194
27	21.799945459682878	27.230115783179244	26.383221295693943	24.58671746144394
28	17.078388563618326	28.155822671687	29.43119541861336	25.334593346081313
29	18.64514952589351	25.615344556810637	28.45957281132336	27.279933105972493
30	18.912594213469486	28.224778388892176	27.480721969234008	25.381905428404334
31	19.232854298499813	26.264776811822767	24.52856302692189	29.973805862755537
32	19.892225376361043	29.031465820306085	25.27734244090918	25.79896636242369
33	19.935882271768488	25.77494069561903	25.94780228806487	28.341374744547608
34	17.947789145819783	26.691735499175323	26.37619840847412	28.984276946530773
35	18.0635230416412	25.6887768512495	27.21787706743943	29.029823039669868
36	17.143031981653426	30.76554399037988	25.263378805501347	26.828045222465352
37	16.141264349688857	26.298987718571965	27.62495810909378	29.934789822645403
38	17.318768440212644	26.03638923387282	27.226049901104606	29.41879242480993
39	18.787989302212495	26.016224101563267	27.942589745106154	27.253196851118076
40	18.17662848844468	23.902335048396317	31.413620951367122	26.507415511791883
41	16.569290844454958	27.26169824091049	27.299112569900313	28.869898344734228
42	18.213385705180016	26.318290391047505	29.58216695908162	25.886156944690864
43	19.29191226237178	27.80365157279818	26.184568047259514	26.71986811757052
44	18.36653392999126	24.378412876771737	27.88332643365466	29.37172675958234
45	19.09859805100505	22.902949448354263	26.562941497295984	31.4355110033447
46	20.86310873236475	25.424494516398237	27.058568415242373	26.65382833599464
47	15.560418186238756	25.970144104717406	32.11512935254729	26.354308356496542
48	17.320329081817047	25.073103738311612	28.513989919898012	29.092577259973325
49	18.100362397408347	22.346827133479213	32.531615313343984	27.021195155768464
50	18.600506962104333	22.9032369349656	28.900905829242813	29.595350273687256
51	17.52181612684895	22.015355070606713	27.641426984971844	32.8214018175725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	812.0
2	1346.0
3	3376.0
4	5406.0
5	3894.5
6	2383.0
7	2581.5
8	2780.0
9	2991.0
10	3202.0
11	3692.5
12	4183.0
13	4298.0
14	4413.0
15	4405.5
16	4398.0
17	4769.0
18	5140.0
19	5641.0
20	6142.0
21	6486.5
22	6831.0
23	8809.5
24	10788.0
25	12069.5
26	16642.5
27	19934.0
28	23123.0
29	26312.0
30	31362.5
31	36413.0
32	43549.0
33	50685.0
34	58845.0
35	67005.0
36	71487.5
37	75970.0
38	85102.5
39	94235.0
40	103987.5
41	113740.0
42	126781.5
43	139823.0
44	166290.5
45	192758.0
46	263804.0
47	334850.0
48	322858.0
49	310866.0
50	286826.5
51	262787.0
52	218348.5
53	173910.0
54	148844.0
55	123778.0
56	108833.5
57	93889.0
58	83114.0
59	72339.0
60	65558.0
61	58777.0
62	50432.0
63	42087.0
64	34669.0
65	27251.0
66	22327.5
67	17404.0
68	14085.5
69	10767.0
70	9008.5
71	7250.0
72	6038.5
73	4827.0
74	4138.0
75	2617.5
76	1786.0
77	1289.0
78	792.0
79	526.5
80	261.0
81	196.0
82	131.0
83	148.0
84	165.0
85	88.0
86	11.0
87	6.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2434896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.094264062984863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.84583578124015	18.382329902308275
2	13.564735550436874	7.3505305389619
3	5.762882642700511	4.684231922559591
4	3.0770921102301374	3.3348618472281064
5	1.7980106387854695	2.4357887517654797
6	1.1747362661544458	1.9097168759732057
7	0.8017495245485541	1.5205969331343763
8	0.5799904680156859	1.2571531915544936
9	0.4704106800241626	1.1470888064360645
>10	3.833818935809382	22.423747867335365
>50	0.8717085722128567	16.499082199865253
>100	0.20331506836416097	8.766971655181507
>500	0.007937879509371411	1.484692197001332
>1k	0.005993909017280454	2.9476578334422765
>5k	0.001133982787053059	2.263863718350816
>10k+	6.479901640303194E-4	3.591685758901998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	26398	1.0841530808708053	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	22581	0.9273907386598853	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	21775	0.8942887088401312	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	11076	0.4548859581682339	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	9818	0.403220507159238	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	9099	0.3736915252232539	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT	8659	0.35562093822487695	No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	7595	0.3119229733015291	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC	5631	0.23126244406331933	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	5579	0.22912682923623842	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	5197	0.21343827416037484	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA	4434	0.182102233524553	No Hit
TCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	4259	0.17491506824110764	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	3636	0.1493287598320421	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	3268	0.13421517797885413	No Hit
TTCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	2878	0.1181980667757473	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	2642	0.1085056610220724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2496	0.1025095116998837	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3855606153199151	0.0
2	0.0	0.0	0.0	1.6257778566312484	0.0
3	0.0	0.0	0.0	2.3063818742155724	0.0
4	0.0	0.0	0.0	3.3308198789599226	0.0
5	0.0	0.0	0.0	5.573215447394879	0.0
6	0.0	0.0	0.0	6.704557402040991	0.0
7	0.0	0.0	0.0	7.823373154335955	0.0
8	0.0	0.0	0.0	9.462457534120555	0.0
9	0.0	0.0	0.0	10.110288078012367	0.0
10	0.0	0.0	0.0	12.443324068050545	0.0
11	0.0	0.0	0.0	14.669538247218773	0.0
12	0.0	0.0	0.0	17.72798509669407	0.0
13	0.0	0.0	0.0	18.42247061065442	0.0
14	4.106951590540212E-5	0.0	0.0	18.701004067524856	0.0
15	4.106951590540212E-5	0.0	0.0	19.221765529205353	0.0
16	4.106951590540212E-5	0.0	0.0	20.122461082526726	0.0
17	8.213903181080424E-5	0.0	0.0	21.362637254322156	0.0
18	8.213903181080424E-5	0.0	0.0	22.637435028025838	0.0
19	8.213903181080424E-5	0.0	0.0	23.98057247619611	0.0
20	8.213903181080424E-5	0.0	0.0	24.88020022210394	0.0
21	8.213903181080424E-5	0.0	0.0	26.17397211215592	0.0
22	8.213903181080424E-5	0.0	0.0	27.485650311142653	0.0
23	8.213903181080424E-5	0.0	0.0	28.82275054047483	0.0
24	8.213903181080424E-5	0.0	0.0	29.85564886549569	0.0
25	8.213903181080424E-5	0.0	0.0	30.71691768354788	0.0
26	8.213903181080424E-5	0.0	0.0	31.454156563565753	0.0
27	1.2320854771620635E-4	0.0	0.0	32.21767993376309	0.0
28	2.053475795270106E-4	0.0	0.0	32.99923282144289	0.0
29	2.053475795270106E-4	0.0	0.0	33.8416918012104	0.0
30	2.464170954324127E-4	0.0	0.0	34.70090714346732	0.0
31	2.464170954324127E-4	0.0	0.0	35.56069745894691	0.0
32	2.464170954324127E-4	0.0	0.0	36.359828099434225	0.0
33	2.464170954324127E-4	0.0	0.0	37.13485093408507	0.0
34	2.464170954324127E-4	0.0	0.0	37.872541578777906	0.0
35	2.464170954324127E-4	0.0	0.0	38.60821981719137	0.0
36	2.8748661133781484E-4	0.0	0.0	39.338230462409896	0.0
37	3.2855612724321697E-4	0.0	0.0	40.09875575794613	0.0
38	3.2855612724321697E-4	0.0	0.0	40.84199078728619	0.0
39	3.2855612724321697E-4	0.0	0.0	41.580502822297134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAA	25	3.8920953E-5	45.000004	21
CGTGCTA	20	7.0348434E-4	45.0	41
AATGCGA	20	7.0348434E-4	45.0	25
CTCGGTA	20	7.0348434E-4	45.0	4
CCGCGTA	20	7.0348434E-4	45.0	40
CGGTAAT	20	7.0348434E-4	45.0	6
CGATCGC	20	7.0348434E-4	45.0	10
CGATCGA	160	0.0	45.0	41
TAATGCG	20	7.0348434E-4	45.0	24
AATACGG	995	0.0	43.190956	5
TAATACG	1000	0.0	42.975002	4
CCCATCG	205	0.0	42.80488	40
TTACGGG	1380	0.0	42.717392	3
TACGCTA	165	0.0	42.272728	38
CGATGAA	3875	0.0	41.864517	19
CTACGGG	670	0.0	41.641792	3
TACGGGA	835	0.0	41.497005	4
TACGAAT	435	0.0	41.37931	12
AGCTACG	420	0.0	41.25	9
TACGGGC	450	0.0	41.0	4
>>END_MODULE