##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1095360
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39616929593924	33.0	31.0	34.0	31.0	34.0
2	32.550308574349984	34.0	31.0	34.0	31.0	34.0
3	32.604037941863865	34.0	31.0	34.0	31.0	34.0
4	36.020277351738244	37.0	35.0	37.0	35.0	37.0
5	31.586267528483788	37.0	35.0	37.0	0.0	37.0
6	33.41561313175577	37.0	35.0	37.0	17.0	37.0
7	35.24624324423021	37.0	35.0	37.0	32.0	37.0
8	35.80547217353199	37.0	35.0	37.0	35.0	37.0
9	37.86617459100204	39.0	38.0	39.0	35.0	39.0
10	37.593918894244815	39.0	37.0	39.0	35.0	39.0
11	37.53140793894245	39.0	37.0	39.0	35.0	39.0
12	37.32510681419807	39.0	37.0	39.0	35.0	39.0
13	37.262128432661406	39.0	37.0	39.0	34.0	39.0
14	38.4693689745837	40.0	38.0	41.0	34.0	41.0
15	38.5693032427695	40.0	38.0	41.0	35.0	41.0
16	38.63679612182296	40.0	38.0	41.0	35.0	41.0
17	38.63051051709027	40.0	38.0	41.0	35.0	41.0
18	38.62510681419807	40.0	38.0	41.0	35.0	41.0
19	38.66209100204499	40.0	38.0	41.0	35.0	41.0
20	38.57628816096991	40.0	38.0	41.0	34.0	41.0
21	38.52842900964067	40.0	38.0	41.0	34.0	41.0
22	38.51393149284254	40.0	38.0	41.0	34.0	41.0
23	38.45608475752264	40.0	38.0	41.0	34.0	41.0
24	38.43545592316681	40.0	37.0	41.0	34.0	41.0
25	38.329614921121824	40.0	37.0	41.0	34.0	41.0
26	38.2745353125913	40.0	37.0	41.0	34.0	41.0
27	38.23278830704061	40.0	37.0	41.0	34.0	41.0
28	38.162003359626055	40.0	37.0	41.0	34.0	41.0
29	38.09022787028922	40.0	37.0	41.0	34.0	41.0
30	38.05444967864447	40.0	37.0	41.0	34.0	41.0
31	37.85917963044113	40.0	36.0	41.0	34.0	41.0
32	37.87087806748466	40.0	36.0	41.0	34.0	41.0
33	37.89159454425942	40.0	36.0	41.0	34.0	41.0
34	37.81501332895121	40.0	36.0	41.0	33.0	41.0
35	37.72414822524102	40.0	36.0	41.0	33.0	41.0
36	37.72092827928717	40.0	36.0	41.0	33.0	41.0
37	37.666644756061935	40.0	36.0	41.0	33.0	41.0
38	37.645040899795504	40.0	36.0	41.0	33.0	41.0
39	37.67714450043821	40.0	36.0	41.0	33.0	41.0
40	37.614993244230206	40.0	36.0	41.0	33.0	41.0
41	37.569853746713406	40.0	36.0	41.0	33.0	41.0
42	37.535680506865326	40.0	35.0	41.0	33.0	41.0
43	37.49514862693544	39.0	35.0	41.0	33.0	41.0
44	37.432651365761025	39.0	35.0	41.0	33.0	41.0
45	37.44173057259714	39.0	35.0	41.0	33.0	41.0
46	37.382000438212096	39.0	35.0	41.0	33.0	41.0
47	37.341457602979844	39.0	35.0	41.0	33.0	41.0
48	37.30002464943033	39.0	35.0	41.0	33.0	41.0
49	37.26872808939527	39.0	35.0	41.0	33.0	41.0
50	37.1759220712825	39.0	35.0	41.0	33.0	41.0
51	36.52445040899796	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	9.0
12	14.0
13	15.0
14	21.0
15	18.0
16	40.0
17	60.0
18	105.0
19	183.0
20	341.0
21	645.0
22	974.0
23	1446.0
24	2140.0
25	3223.0
26	4429.0
27	5618.0
28	7097.0
29	9406.0
30	12320.0
31	16949.0
32	23410.0
33	35591.0
34	60358.0
35	89259.0
36	87479.0
37	139217.0
38	231649.0
39	362738.0
40	591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.839796961729476	4.993883289512124	56.577837423312886	15.588482325445515
2	18.682716184633364	4.978271983640082	54.384220712825005	21.954791118901547
3	20.052128980426527	4.607069821793748	53.50943981887234	21.831361378907392
4	18.459045427987146	4.887616856558575	52.64588810984516	24.007449605609114
5	15.917141396435873	17.125419953257374	44.48318361086766	22.47425503943909
6	19.990870581361378	6.413234005258545	54.03255550686532	19.563339906514756
7	82.53414402570844	2.229769208296816	9.674079754601227	5.562007011393515
8	85.42981302950629	2.85394756061934	6.49987218813906	5.21636722173532
9	80.38507887817704	4.694620946538124	8.825774174700555	6.094526000584283
10	37.03960341805434	27.468868682442306	17.917853491089687	17.573674408413673
11	27.86408121530821	28.04110064271107	24.332456909144025	19.762361232836692
12	24.533943178498394	23.699149138182882	30.772257522640956	20.994650160677768
13	22.032391177329828	25.87441571720713	30.70168711656442	21.391505988898626
14	17.849930616418348	27.703677329827634	31.34540242477359	23.100989628980425
15	16.97880148992112	25.43319091440257	35.51681638913234	22.071191206543965
16	18.682259713701434	24.804904323692668	32.688248612328366	23.824587350277536
17	18.451102833771547	23.537284545720127	30.772896581945663	27.238716038562664
18	20.25032865907099	23.556730207420394	32.50748612328367	23.68545501022495
19	21.73623283669296	24.950244668419515	29.850186240140225	23.463336254747297
20	24.102304265264387	25.34500073035349	29.80773444347064	20.744960560911483
21	22.317502921413965	27.483658340636868	28.891505988898626	21.307332749050538
22	21.074623867952088	25.226866053169733	28.64017309377739	25.058336985100787
23	20.444146216768917	26.177055945077417	28.522677475898334	24.856120362255332
24	21.689125036517677	24.697998831434415	30.11968667835232	23.49318945369559
25	20.033139789658193	27.182478819748756	28.013803680981596	24.77057770961145
26	19.51449751679813	28.323382267017237	27.316316096990946	24.84580411919369
27	20.86829900671925	27.260717937481743	28.828056529360207	23.042926526438794
28	18.455211072158924	26.94127957931639	31.927859333917617	22.675650014607072
29	18.94975167981303	26.02642053754017	30.012324715162137	25.011503067484664
30	20.72807113643003	26.98993938066024	29.077746129126496	23.20424335378323
31	22.983859187846917	28.435765410458664	26.280400964066608	22.29997443762781
32	22.128615249780896	29.071812007011395	25.867751241600935	22.931821501606777
33	23.561477505112475	27.283997955010225	25.268131025416302	23.886393514461
34	21.396801051709026	25.828312153082088	28.79217791411043	23.982708881098453
35	21.17605170902717	28.056438066023954	26.004601226993866	24.76290899795501
36	23.874251387671634	26.984096552731522	26.962551124744376	22.17910093485247
37	22.400854513584576	27.93419515045282	28.603655419222907	21.0612949167397
38	19.86899284253579	28.795738387379494	27.671541776219687	23.66372699386503
39	21.78489263803681	26.16737876132048	28.178863569967866	23.868865030674847
40	23.108749634823255	24.965855974291557	28.34675357873211	23.578640812153083
41	21.403374233128837	26.25191717791411	26.58514095822378	25.759567630733276
42	22.026913526146654	26.005879345603272	27.6655163599182	24.301690768331873
43	22.541356266432956	24.802804557405782	28.863661262050833	23.79217791411043
44	20.889935728892784	24.87346625766871	28.492550394390886	25.744047619047617
45	20.855243938066025	26.48444347063979	26.11598013438504	26.544332456909142
46	21.80999853929302	26.582676015191353	27.611196319018404	23.996129126497227
47	19.540151183172654	26.27674919661116	30.17619777972539	24.006901840490798
48	19.785641250365178	25.54374817411627	30.32929813029506	24.341312445223487
49	21.94529652351738	23.781405200116858	30.56903666374525	23.70426161262051
50	21.100094945953842	23.986908413672218	28.94591732398481	25.967079316389132
51	20.3330411919369	24.59118463336255	27.14431784983932	27.93145632486123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	469.5
2	754.0
3	1280.5
4	1807.0
5	1336.0
6	865.0
7	978.5
8	1092.0
9	1340.5
10	1589.0
11	1547.0
12	1505.0
13	1590.0
14	1675.0
15	1683.0
16	1691.0
17	1720.5
18	1750.0
19	2354.0
20	2958.0
21	3453.0
22	3948.0
23	4116.5
24	4285.0
25	5105.5
26	9095.0
27	12264.0
28	14171.5
29	16079.0
30	17889.0
31	19699.0
32	23294.5
33	26890.0
34	29882.5
35	32875.0
36	36704.0
37	40533.0
38	43259.0
39	45985.0
40	51638.5
41	57292.0
42	63862.5
43	70433.0
44	77556.0
45	84679.0
46	96640.0
47	108601.0
48	117483.5
49	126366.0
50	118720.0
51	111074.0
52	94871.5
53	78669.0
54	68034.0
55	57399.0
56	51003.5
57	44608.0
58	40811.5
59	37015.0
60	33408.0
61	29801.0
62	26122.5
63	22444.0
64	18812.5
65	15181.0
66	12423.0
67	9665.0
68	8396.5
69	7128.0
70	5786.0
71	4444.0
72	3640.0
73	2836.0
74	2198.0
75	1166.5
76	773.0
77	608.0
78	443.0
79	358.5
80	274.0
81	206.5
82	139.0
83	150.5
84	162.0
85	90.5
86	19.0
87	9.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1095360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.0897908528527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63248049168918	16.292523099044327
2	12.673803813544597	6.106185663567526
3	5.434384359807588	3.927395479253358
4	2.920258022054038	2.8139361995058834
5	1.818817971137715	2.190747226205872
6	1.2299202057732872	1.7777112313665626
7	0.9494916909053418	1.6011139375301802
8	0.7059508386305795	1.3604966444005302
9	0.5576893793809697	1.2091158459130307
>10	4.173410227824112	21.796102375350657
>50	1.5982223771274513	27.50677440345104
>100	0.2948622649042818	10.000729421020162
>500	0.006883943927726209	1.1072101241058816
>1k	0.0038244132931812267	2.3099583492849702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT	4728	0.4316389132340053	No Hit
TCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	4205	0.38389205375401697	No Hit
CCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	4012	0.36627227578147825	No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	3401	0.31049152789950335	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2354	0.21490651475314054	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTG	1588	0.14497516798130294	No Hit
TTTTTTGGGATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTA	1275	0.11640008764241894	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1255	0.11457420391469471	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTG	1232	0.11247443762781187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8258837277242187E-4	0.0	0.0	0.4485283377154543	0.0
2	1.8258837277242187E-4	0.0	0.0	1.5913489628980426	0.0
3	1.8258837277242187E-4	0.0	0.0	2.2304082676015193	0.0
4	1.8258837277242187E-4	0.0	0.0	2.9513584574934266	0.0
5	1.8258837277242187E-4	0.0	0.0	4.155437481741163	0.0
6	1.8258837277242187E-4	0.0	0.0	5.006573181419808	0.0
7	1.8258837277242187E-4	0.0	0.0	5.74906879929886	0.0
8	1.8258837277242187E-4	0.0	0.0	6.92174262342974	0.0
9	1.8258837277242187E-4	0.0	0.0	7.524010371019574	0.0
10	1.8258837277242187E-4	0.0	0.0	8.671852176453404	0.0
11	1.8258837277242187E-4	0.0	0.0	10.412101957347357	0.0
12	1.8258837277242187E-4	0.0	0.0	11.961638182880515	0.0
13	1.8258837277242187E-4	0.0	0.0	12.44485831142273	0.0
14	1.8258837277242187E-4	0.0	0.0	12.635297984224364	0.0
15	1.8258837277242187E-4	0.0	0.0	12.948984808647385	0.0
16	1.8258837277242187E-4	0.0	0.0	13.654506281040023	0.0
17	1.8258837277242187E-4	0.0	0.0	14.64340490797546	0.0
18	1.8258837277242187E-4	0.0	0.0	15.798185071574641	0.0
19	1.8258837277242187E-4	0.0	0.0	16.53721151037102	0.0
20	1.8258837277242187E-4	0.0	0.0	17.322250219106046	0.0
21	1.8258837277242187E-4	0.0	0.0	18.312609553023663	0.0
22	1.8258837277242187E-4	0.0	0.0	19.2909180543383	0.0
23	2.738825591586328E-4	0.0	0.0	20.238643003213557	0.0
24	2.738825591586328E-4	0.0	0.0	20.975113204791118	0.0
25	2.738825591586328E-4	0.0	0.0	21.6148115687993	0.0
26	2.738825591586328E-4	0.0	0.0	22.1954425942156	0.0
27	2.738825591586328E-4	0.0	0.0	22.807387525562373	0.0
28	2.738825591586328E-4	0.0	0.0	23.386831726555652	0.0
29	2.738825591586328E-4	0.0	0.0	24.0386722173532	0.0
30	2.738825591586328E-4	0.0	0.0	24.767930178206253	0.0
31	2.738825591586328E-4	0.0	0.0	25.398955594507743	0.0
32	2.738825591586328E-4	0.0	0.0	26.02705959684487	0.0
33	2.738825591586328E-4	0.0	0.0	26.589340490797547	0.0
34	2.738825591586328E-4	0.0	0.0	27.190512708150745	0.0
35	2.738825591586328E-4	0.0	0.0	27.818342827928717	0.0
36	2.738825591586328E-4	0.0	0.0	28.39176161262051	0.0
37	2.738825591586328E-4	0.0	0.0	28.99046888694128	0.0
38	2.738825591586328E-4	0.0	0.0	29.575573327490506	0.0
39	2.738825591586328E-4	0.0	0.0	30.222757814782355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	30	2.1654068E-6	45.000004	1
CGACGGT	30	2.1654068E-6	45.000004	28
CTACGAT	30	2.1654068E-6	45.000004	44
ACCGTAC	30	2.1654068E-6	45.000004	11
GAACCGC	30	2.1654068E-6	45.000004	9
CCGCCGT	30	2.1654068E-6	45.000004	38
CGAACAG	20	7.0331414E-4	45.0	39
CGTATGG	195	0.0	45.0	2
CGAAATG	25	3.890685E-5	45.0	19
GATCGAA	35	1.2118653E-7	45.0	9
CTATCGT	25	3.890685E-5	45.0	31
ATCAGCG	20	7.0331414E-4	45.0	12
CGTTGCA	20	7.0331414E-4	45.0	41
GATCCGA	20	7.0331414E-4	45.0	10
GGTCCAC	35	1.2118653E-7	45.0	8
CGACCTA	25	3.890685E-5	45.0	29
ACCGAAC	20	7.0331414E-4	45.0	11
CGACCGA	20	7.0331414E-4	45.0	38
TCGATCC	20	7.0331414E-4	45.0	17
TATTGCG	80	0.0	45.0	1
>>END_MODULE