SRR2935320.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1282620
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	35133	2.73915890910792	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	28803	2.245637835056369	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	26364	2.055480188988165	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	12698	0.9900048338557016	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	7645	0.5960455941744243	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTC	7494	0.5842728165785657	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	7085	0.5523849620308431	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	6625	0.516520871341473	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	6559	0.5113751539816938	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	4973	0.38772200651790867	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	4209	0.3281564298077373	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	4031	0.31427858601924186	No Hit
GAATATGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	3703	0.28870593004943007	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3176	0.24761815658573857	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3013	0.2349097940153748	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	2497	0.19467964011164646	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATG	2088	0.16279178556392385	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1712	0.1334767896960908	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	1707	0.13308696262338027	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	1543	0.12030063463847437	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	1521	0.11858539551854796	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGAGAACT	1434	0.11180240445338448	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCGAGAACTT	1385	0.10798209914082114	No Hit
TGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCTT	1356	0.10572110211909996	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCGAGAACAT	1319	0.10283638178104193	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	40	6.8157533E-9	45.0	1
TCGCACA	20	7.0335943E-4	45.0	24
ACGTTAC	25	3.8910595E-5	45.0	13
CGTGCCA	25	3.8910595E-5	45.0	31
ACGCAAT	20	7.0335943E-4	45.0	35
TCCGAAT	20	7.0335943E-4	45.0	39
TTCGTAG	45	3.8562575E-10	45.0	1
CGCGATA	20	7.0335943E-4	45.0	29
TACGGGT	95	0.0	45.0	4
TAACCGA	20	7.0335943E-4	45.0	41
TGATTCG	20	7.0335943E-4	45.0	1
AACGCGT	20	7.0335943E-4	45.0	24
GTATCGT	20	7.0335943E-4	45.0	9
CGAGCGT	25	3.8910595E-5	45.0	39
CGTAATC	25	3.8910595E-5	45.0	43
CCGCGTA	90	0.0	45.0	40
CACGTCG	20	7.0335943E-4	45.0	32
GCGTCGA	20	7.0335943E-4	45.0	21
TTATTCG	20	7.0335943E-4	45.0	1
AGTACGC	25	3.8910595E-5	45.0	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content