##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1282620
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05079758619077	33.0	31.0	34.0	30.0	34.0
2	32.178108870904865	33.0	31.0	34.0	30.0	34.0
3	32.278796525861125	34.0	31.0	34.0	30.0	34.0
4	35.866188738675525	37.0	35.0	37.0	35.0	37.0
5	31.56916000062372	37.0	35.0	37.0	0.0	37.0
6	32.88497138669286	37.0	33.0	37.0	17.0	37.0
7	34.957253902169	37.0	35.0	37.0	32.0	37.0
8	35.3147019382202	37.0	35.0	37.0	33.0	37.0
9	37.39647440395441	39.0	37.0	39.0	34.0	39.0
10	37.24877360402925	39.0	37.0	39.0	34.0	39.0
11	37.15635885921005	39.0	37.0	39.0	34.0	39.0
12	36.93966646395659	39.0	35.0	39.0	33.0	39.0
13	36.70561506915532	39.0	35.0	39.0	32.0	39.0
14	37.88622351125041	40.0	37.0	41.0	33.0	41.0
15	37.83869657419969	40.0	36.0	41.0	33.0	41.0
16	38.0603265191561	40.0	37.0	41.0	33.0	41.0
17	37.833517331711654	40.0	36.0	41.0	33.0	41.0
18	37.92009870421481	40.0	36.0	41.0	33.0	41.0
19	37.89188302069202	40.0	36.0	41.0	33.0	41.0
20	37.37507757558747	40.0	35.0	41.0	31.0	41.0
21	37.66590416491245	40.0	36.0	41.0	32.0	41.0
22	37.683663906690995	40.0	36.0	41.0	33.0	41.0
23	37.159895370413686	39.0	35.0	41.0	31.0	41.0
24	37.341249161871794	39.0	35.0	41.0	31.0	41.0
25	37.51908047590089	39.0	35.0	41.0	33.0	41.0
26	37.416946562504876	39.0	35.0	41.0	32.0	41.0
27	37.06857993793953	39.0	35.0	41.0	31.0	41.0
28	37.12244390388424	39.0	35.0	41.0	31.0	41.0
29	37.097787341535295	39.0	35.0	41.0	31.0	41.0
30	37.06737381297656	39.0	35.0	41.0	31.0	41.0
31	36.82818371770283	39.0	35.0	41.0	30.0	41.0
32	36.41133305265784	39.0	35.0	41.0	29.0	41.0
33	36.16668303940372	39.0	35.0	41.0	28.0	41.0
34	36.15294007578238	39.0	35.0	41.0	29.0	41.0
35	36.09976532410223	39.0	35.0	41.0	29.0	41.0
36	36.09371676724205	39.0	35.0	41.0	29.0	41.0
37	35.79443872698071	39.0	35.0	41.0	27.0	41.0
38	35.76083173504234	39.0	35.0	41.0	26.0	41.0
39	35.73982941167298	39.0	35.0	41.0	27.0	41.0
40	35.72613868487939	39.0	35.0	41.0	26.0	41.0
41	35.62491228890864	38.0	35.0	41.0	26.0	41.0
42	35.594229000015595	39.0	35.0	41.0	26.0	41.0
43	35.4875192964401	39.0	35.0	41.0	26.0	41.0
44	35.62355802965804	39.0	35.0	41.0	26.0	41.0
45	35.48855311783693	39.0	35.0	41.0	26.0	41.0
46	35.374778968049775	38.0	35.0	40.0	26.0	41.0
47	35.200983923531524	38.0	35.0	40.0	25.0	41.0
48	35.28726045126382	38.0	35.0	40.0	26.0	41.0
49	35.31247134771016	38.0	35.0	40.0	26.0	41.0
50	35.14095133398824	38.0	35.0	40.0	26.0	41.0
51	34.732078090159206	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	5.0
12	9.0
13	18.0
14	23.0
15	26.0
16	47.0
17	93.0
18	244.0
19	687.0
20	2188.0
21	5282.0
22	9428.0
23	12752.0
24	15978.0
25	18274.0
26	17251.0
27	15096.0
28	13720.0
29	15495.0
30	19951.0
31	26550.0
32	35650.0
33	50557.0
34	78071.0
35	109455.0
36	105833.0
37	158221.0
38	241318.0
39	330001.0
40	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94651572562412	3.3269401693408804	43.63848996585114	13.088054139183857
2	15.850602672654409	24.290280831423182	42.11520169652742	17.74391479939499
3	17.467995197330463	24.429527061795387	40.012396500912196	18.090081239961954
4	15.853331462163384	4.141132993404126	59.278586019241864	20.726949525190623
5	27.87840513948013	16.167999875255337	35.36542389795886	20.58817108730567
6	17.07185292604201	22.439771717266222	44.13606524145889	16.352310115232882
7	68.4204986667914	1.9925620994526827	24.938407322511733	4.648531911244172
8	67.46643588903962	18.180053328343547	8.328187615973553	6.025323166643277
9	63.28959473577521	4.555986964182688	8.934758541111162	23.219659758930938
10	45.2721772621665	22.47080195225398	16.456627839890224	15.800392945689293
11	37.95551293446227	20.34593254432334	23.046888400305622	18.651666120908764
12	24.725951567884486	17.2047839578363	26.65536168155806	31.41390279272115
13	28.082518594751367	21.050428030125836	32.98716689276637	17.879886482356426
14	19.95267499337294	33.07518984578441	24.672077466435887	22.30005769440676
15	16.293914019740843	20.308431179928586	36.65044986044191	26.747204939888665
16	16.999734917590555	22.110445806240353	27.832483510314827	33.05733576585427
17	17.925652180692644	27.57543153856949	30.379379707161903	24.11953657357596
18	17.68146450234676	21.444309304392572	29.615474575478316	31.258751617782355
19	18.642622132821877	24.166705649373938	26.91568820071416	30.274984017090016
20	27.749684240071105	23.077606773635214	31.701361276137906	17.471347710155776
21	22.03676848949806	34.39600193354228	24.705446663859913	18.86178291309975
22	19.069794639098095	21.634311019631692	34.312812836225845	24.983081505044364
23	25.48977873415353	27.498479674416426	25.70777003321327	21.303971558216777
24	21.349347429480282	27.020551683273297	25.030874304158672	26.59922658308774
25	18.152687467839264	35.72367497777986	23.48263710218147	22.641000452199407
26	18.14403330682509	22.283606991938377	32.533408180131296	27.038951521105236
27	27.330620136907267	24.568851257582136	24.11088241256179	23.989646192948808
28	15.751352699942306	30.47504327080507	30.43652835602127	23.337075673231354
29	21.967925028457376	20.43114874241786	30.335797040432865	27.265129188691894
30	21.622694172864918	33.10559635745583	25.847094229000017	19.424615240679234
31	23.776566715005224	25.827758806193575	20.35973242269729	30.035942056103913
32	32.03567697369447	26.962311518610342	20.60727261386849	20.3947388938267
33	26.28970388735557	25.828070667851744	21.195911493661413	26.68631395113128
34	22.25974957508849	25.31170572733935	22.52950990940419	29.899034788167967
35	26.305141039434908	21.351296564843835	23.70133008997209	28.64223230574917
36	18.392820944628962	31.512607007531457	24.782242597183888	25.31232945065569
37	25.347102025541467	24.125150083422994	26.05916015655455	24.468587734480984
38	20.396454132946626	29.311877251251346	20.916951240429746	29.374717375372285
39	24.241708378163445	30.382030531256333	24.298623130779188	21.07763795980103
40	19.51240429745365	25.404640501473548	25.20364566278399	29.87930953828882
41	18.55569069560743	27.46308337621431	24.555129344622724	29.426096583555534
42	21.691381703076516	20.395362617143036	27.912943818122283	30.00031186165817
43	21.713445915391933	24.281158877921754	26.177511655829473	27.827883550856843
44	23.302692925418285	22.148181066878735	28.65696776909763	25.892158238605354
45	20.761410238418236	22.64201400258845	25.09956183437027	31.497013924623037
46	28.701174159143005	25.66902122218584	23.57978200870094	22.050022609970217
47	16.390435202943973	27.580889117587436	33.42525455707848	22.603421122390106
48	20.602750619825045	27.264973257862813	25.7148648859366	26.417411236375543
49	21.466607412951618	19.50242472439226	33.33395705664967	25.697010806006453
50	22.200106032963777	22.389717921130188	28.832234020988288	26.577942024917746
51	19.295114687124794	21.595952035676973	27.274640969266034	31.8342923079322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	226.0
1	592.5
2	959.0
3	1582.5
4	2206.0
5	1654.5
6	1103.0
7	1210.5
8	1318.0
9	1428.5
10	1539.0
11	1591.0
12	1643.0
13	1690.0
14	1737.0
15	1673.0
16	1609.0
17	1777.5
18	1946.0
19	1986.5
20	2027.0
21	2392.0
22	2757.0
23	3459.5
24	4162.0
25	4475.5
26	6645.5
27	8502.0
28	9437.5
29	10373.0
30	12594.0
31	14815.0
32	16691.5
33	18568.0
34	20634.0
35	22700.0
36	25532.0
37	28364.0
38	31188.0
39	34012.0
40	39161.5
41	44311.0
42	50168.0
43	56025.0
44	71544.0
45	87063.0
46	153726.0
47	220389.0
48	182328.0
49	144267.0
50	136795.0
51	129323.0
52	113146.0
53	96969.0
54	90704.0
55	84439.0
56	75821.0
57	67203.0
58	60124.0
59	53045.0
60	47707.5
61	42370.0
62	36824.5
63	31279.0
64	25848.5
65	20418.0
66	17334.5
67	14251.0
68	11949.0
69	9647.0
70	8083.0
71	6519.0
72	5655.0
73	4791.0
74	3551.0
75	1704.5
76	1098.0
77	873.0
78	648.0
79	553.0
80	458.0
81	351.0
82	244.0
83	213.5
84	183.0
85	98.5
86	14.0
87	7.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1282620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.81393177909737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.27575925545774	15.111766863437284
2	11.955843485819633	5.216079083224703
3	5.07326944198172	3.3200386051310593
4	2.762201157595105	2.4101787044769365
5	1.6919232912157842	1.8453749625023503
6	1.1839885150643088	1.5496466816908578
7	0.8582501989834653	1.310526790301544
8	0.648372881145707	1.1314849437383407
9	0.5430248745006346	1.0660956810038777
>10	4.27731082156891	20.795709455497004
>50	1.454934642834837	22.24095297990263
>100	0.2574530848704448	8.400027198681045
>500	0.006129835354056948	0.9876092430906624
>1k	0.008293306655488813	3.7279967892262693
>5k	0.0018028927511932202	2.7850643580243846
>10k+	0.0014423142009545762	8.101447660071045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	35133	2.73915890910792	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	28803	2.245637835056369	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	26364	2.055480188988165	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	12698	0.9900048338557016	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	7645	0.5960455941744243	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTC	7494	0.5842728165785657	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	7085	0.5523849620308431	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	6625	0.516520871341473	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	6559	0.5113751539816938	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	4973	0.38772200651790867	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	4209	0.3281564298077373	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	4031	0.31427858601924186	No Hit
GAATATGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	3703	0.28870593004943007	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3176	0.24761815658573857	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3013	0.2349097940153748	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	2497	0.19467964011164646	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATG	2088	0.16279178556392385	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1712	0.1334767896960908	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	1707	0.13308696262338027	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	1543	0.12030063463847437	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	1521	0.11858539551854796	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGAGAACT	1434	0.11180240445338448	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCGAGAACTT	1385	0.10798209914082114	No Hit
TGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCTT	1356	0.10572110211909996	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCGAGAACAT	1319	0.10283638178104193	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3389624362632735E-4	0.0	0.0	0.3277666027350267	0.0
2	2.3389624362632735E-4	0.0	0.0	1.4719870265550201	0.0
3	2.3389624362632735E-4	0.0	0.0	1.9175593706631737	0.0
4	2.3389624362632735E-4	0.0	0.0	3.000343047823985	0.0
5	2.3389624362632735E-4	0.0	0.0	5.979323572063433	0.0
6	2.3389624362632735E-4	0.0	0.0	6.627683959395612	0.0
7	2.3389624362632735E-4	0.0	0.0	7.7442266610531565	0.0
8	2.3389624362632735E-4	0.0	0.0	9.229467808080335	0.0
9	2.3389624362632735E-4	0.0	0.0	9.623894840248866	0.0
10	2.3389624362632735E-4	0.0	0.0	13.102321810045064	0.0
11	2.3389624362632735E-4	0.0	0.0	14.391012147011585	0.0
12	2.3389624362632735E-4	0.0	0.0	19.031357689728836	0.0
13	2.3389624362632735E-4	0.0	0.0	19.644945502175236	0.0
14	2.3389624362632735E-4	0.0	0.0	19.95641733327096	0.0
15	2.3389624362632735E-4	0.0	0.0	20.838440067985843	0.0
16	2.3389624362632735E-4	0.0	0.0	21.577474232430493	0.0
17	2.3389624362632735E-4	0.0	0.0	22.36508085013488	0.0
18	2.3389624362632735E-4	0.0	0.0	23.094915095663563	0.0
19	2.3389624362632735E-4	0.0	0.0	24.92226848170152	0.0
20	2.3389624362632735E-4	0.0	0.0	25.617953875660756	0.0
21	2.3389624362632735E-4	0.0	0.0	26.361587999563394	0.0
22	2.3389624362632735E-4	0.0	0.0	27.138669286304594	0.0
23	2.3389624362632735E-4	0.0	0.0	27.81899549359904	0.0
24	2.3389624362632735E-4	0.0	0.0	28.39461414916343	0.0
25	2.3389624362632735E-4	0.0	0.0	28.90099951661443	0.0
26	2.3389624362632735E-4	0.0	0.0	29.363724251921848	0.0
27	2.3389624362632735E-4	0.0	0.0	29.85100809281003	0.0
28	2.3389624362632735E-4	0.0	0.0	30.318488718404517	0.0
29	2.3389624362632735E-4	0.0	0.0	30.795948917060393	0.0
30	2.3389624362632735E-4	0.0	0.0	31.321669707317835	0.0
31	2.3389624362632735E-4	0.0	0.0	31.810512856496857	0.0
32	2.3389624362632735E-4	0.0	0.0	32.28851881305453	0.0
33	2.3389624362632735E-4	0.0	0.0	32.763640049274144	0.0
34	2.3389624362632735E-4	0.0	0.0	33.234551153108484	0.0
35	3.118616581684365E-4	0.0	0.0	33.70631987650278	0.0
36	3.118616581684365E-4	0.0	0.0	34.16054638162511	0.0
37	3.118616581684365E-4	0.0	0.0	34.60931530772949	0.0
38	3.898270727105456E-4	0.0	0.0	35.07671796790944	0.0
39	3.898270727105456E-4	0.0	0.0	35.55261885827447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	40	6.8157533E-9	45.0	1
TCGCACA	20	7.0335943E-4	45.0	24
ACGTTAC	25	3.8910595E-5	45.0	13
CGTGCCA	25	3.8910595E-5	45.0	31
ACGCAAT	20	7.0335943E-4	45.0	35
TCCGAAT	20	7.0335943E-4	45.0	39
TTCGTAG	45	3.8562575E-10	45.0	1
CGCGATA	20	7.0335943E-4	45.0	29
TACGGGT	95	0.0	45.0	4
TAACCGA	20	7.0335943E-4	45.0	41
TGATTCG	20	7.0335943E-4	45.0	1
AACGCGT	20	7.0335943E-4	45.0	24
GTATCGT	20	7.0335943E-4	45.0	9
CGAGCGT	25	3.8910595E-5	45.0	39
CGTAATC	25	3.8910595E-5	45.0	43
CCGCGTA	90	0.0	45.0	40
CACGTCG	20	7.0335943E-4	45.0	32
GCGTCGA	20	7.0335943E-4	45.0	21
TTATTCG	20	7.0335943E-4	45.0	1
AGTACGC	25	3.8910595E-5	45.0	38
>>END_MODULE