##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020615
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39951695791263	33.0	31.0	34.0	31.0	34.0
2	32.53954625397432	34.0	31.0	34.0	31.0	34.0
3	32.60110227656854	34.0	31.0	34.0	31.0	34.0
4	36.02122837700798	37.0	35.0	37.0	35.0	37.0
5	31.64230292519706	37.0	35.0	37.0	0.0	37.0
6	33.40125610538744	37.0	35.0	37.0	17.0	37.0
7	35.219772392136115	37.0	35.0	37.0	32.0	37.0
8	35.77943396873454	37.0	35.0	37.0	35.0	37.0
9	37.823314374176356	39.0	38.0	39.0	35.0	39.0
10	37.59691852461506	39.0	37.0	39.0	35.0	39.0
11	37.523760673711436	39.0	37.0	39.0	35.0	39.0
12	37.28544358058622	39.0	37.0	39.0	35.0	39.0
13	37.19030584500522	39.0	37.0	39.0	34.0	39.0
14	38.40336561778928	40.0	38.0	41.0	34.0	41.0
15	38.505527549565706	40.0	38.0	41.0	34.0	41.0
16	38.570371785639054	40.0	38.0	41.0	35.0	41.0
17	38.56306932584765	40.0	38.0	41.0	35.0	41.0
18	38.54005379109655	40.0	38.0	41.0	35.0	41.0
19	38.545722921963716	40.0	38.0	41.0	34.0	41.0
20	38.46630805935637	40.0	37.0	41.0	34.0	41.0
21	38.405201765602115	40.0	37.0	41.0	34.0	41.0
22	38.37059322075415	40.0	37.0	41.0	34.0	41.0
23	38.3275044948389	40.0	37.0	41.0	34.0	41.0
24	38.315476452922994	40.0	37.0	41.0	34.0	41.0
25	38.23449586768762	40.0	37.0	41.0	34.0	41.0
26	38.164136329566	40.0	37.0	41.0	34.0	41.0
27	38.11860593857625	40.0	37.0	41.0	34.0	41.0
28	38.06299829024657	40.0	37.0	41.0	34.0	41.0
29	38.003068737966814	40.0	36.0	41.0	34.0	41.0
30	37.96529445481401	40.0	36.0	41.0	34.0	41.0
31	37.750710111060485	40.0	36.0	41.0	33.0	41.0
32	37.77592236053752	40.0	36.0	41.0	33.0	41.0
33	37.76174169495843	40.0	36.0	41.0	33.0	41.0
34	37.67284235485467	40.0	36.0	41.0	33.0	41.0
35	37.59021766287974	40.0	36.0	41.0	33.0	41.0
36	37.58132204602127	40.0	35.0	41.0	33.0	41.0
37	37.50736859638551	40.0	35.0	41.0	33.0	41.0
38	37.50985337272135	39.0	35.0	41.0	33.0	41.0
39	37.507363697378544	39.0	35.0	41.0	33.0	41.0
40	37.38104280262391	39.0	35.0	41.0	33.0	41.0
41	37.32978253308055	39.0	35.0	41.0	33.0	41.0
42	37.29566290912832	39.0	35.0	41.0	33.0	41.0
43	37.22284896851408	39.0	35.0	41.0	33.0	41.0
44	37.1577039334127	39.0	35.0	41.0	33.0	41.0
45	37.15583545215385	39.0	35.0	41.0	33.0	41.0
46	37.0851555189763	39.0	35.0	41.0	33.0	41.0
47	37.041826741719454	39.0	35.0	41.0	33.0	41.0
48	36.99598281428354	39.0	35.0	41.0	33.0	41.0
49	36.9572933966285	39.0	35.0	41.0	33.0	41.0
50	36.852892618666196	39.0	35.0	41.0	32.0	41.0
51	36.21406210961038	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	12.0
11	12.0
12	13.0
13	19.0
14	22.0
15	30.0
16	49.0
17	69.0
18	133.0
19	221.0
20	373.0
21	634.0
22	981.0
23	1371.0
24	2169.0
25	3090.0
26	4381.0
27	5665.0
28	7151.0
29	9209.0
30	12067.0
31	16370.0
32	22755.0
33	35120.0
34	59798.0
35	88853.0
36	86355.0
37	136349.0
38	214046.0
39	312938.0
40	353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.550702272649335	4.491311611136423	55.484389314286	16.47359680192825
2	19.470907247101014	4.500227803824164	54.28736595092175	21.741498998153077
3	21.748651548331154	4.785643949971341	50.252151888812136	23.21355261288537
4	19.605629938811404	5.465822077864817	49.77802599413099	25.150521989192793
5	16.760580630306237	17.731857752433584	41.98321600211637	23.52434561514381
6	21.374759336282537	7.122274314996351	51.23508864753114	20.267877701189967
7	82.62351621326357	2.9920195176437736	8.979977758508351	5.404486510584305
8	83.97456435580509	3.3500389471054213	7.304811314746501	5.370585382342999
9	78.5100160197528	5.594861921488514	9.448616765381656	6.446505293377032
10	38.95190644856288	28.134997036100785	16.79595146063893	16.117145054697414
11	30.91028448533482	25.076449003786934	23.916070212567913	20.097196298310333
12	27.532517158771917	22.59412217143585	29.646046746324522	20.22731392346771
13	23.59881052110737	24.625544402149686	31.521974495769705	20.25367058097324
14	20.3749699935823	26.872228999181868	31.196288512318553	21.55651249491728
15	17.588806748872006	24.624172680197724	35.88934123053257	21.8976793403977
16	19.314922865135237	24.223433909946454	34.31470240982153	22.14694081509678
17	19.994415132052733	23.16730598707642	29.905792095942154	26.932486784928695
18	22.078256737359336	23.42019272693425	31.121823606354994	23.37972692935142
19	25.222439411531283	23.74274334592378	28.356824071760656	22.677993170784283
20	27.024686096128313	24.96867085041862	27.54927176261372	20.457371290839347
21	26.03283314472156	24.47847621287165	27.969900501168414	21.51879014123837
22	23.631535887675568	23.54345174233183	27.31656893147759	25.50844343851501
23	23.047770216977018	25.85382342999074	26.768174091111728	24.33023226192051
24	23.48045051268108	23.374827922380135	28.348495759909465	24.79622580502932
25	22.005849414323716	25.23860613453653	27.08318023936548	25.672364211774273
26	20.629130475252666	28.798028639594754	26.987747583564815	23.58509330158777
27	22.04190610563239	27.420525859408297	27.5933628253553	22.944205209604014
28	19.690186799135816	28.340167448058278	29.40374186152467	22.565903891281238
29	21.677811907526344	26.175590207864868	28.9862484874316	23.160349397177193
30	22.756769202882577	25.574873973045666	28.171543628106583	23.496813195965178
31	23.920773259260347	28.506635704942607	24.461133728193296	23.11145730760375
32	24.80935514371237	27.79961101884648	24.92869495353292	22.46233888390823
33	24.28849272252514	26.935426189111467	25.36970356108817	23.40637752727522
34	22.557477599290625	27.49557864620841	26.710463788989973	23.23647996551099
35	22.600490880498523	26.18382053957663	26.31589776752252	24.899790812402326
36	23.867080142855045	27.503906958059606	26.42426380172739	22.204749097357965
37	22.82378761824978	27.606786104456628	27.42571880679786	22.14370747049573
38	23.30712364603695	28.234642837896757	24.676396094511645	23.78183742155465
39	24.815723852775044	24.903416077561076	25.857154754731216	24.423705314932665
40	24.5612694306864	25.08634499786893	26.947085825703134	23.405299745741537
41	21.73248482532591	26.519794437667482	27.194191737334844	24.553528999671766
42	24.536088534853988	25.649632819427502	25.90349936067959	23.91077928503892
43	24.555488602460283	24.232448082773622	27.41866423675921	23.79339907800689
44	22.643700121985276	25.7288007720835	27.418762216898635	24.208736889032593
45	22.817320929047682	25.087422779402612	25.379109654473037	26.716146637076665
46	22.677013369390025	26.50940854288836	26.955610097833173	23.857967989888447
47	21.155871704805435	27.066817556081386	28.565815709155757	23.211495029957426
48	22.645365784355512	25.25006981084934	28.535049945376073	23.569514459419075
49	23.765082817712848	23.072951112809434	29.2717626137182	23.89020345575952
50	22.675837607716918	24.251946130519343	27.29217187676058	25.78004438500316
51	21.41787059762986	24.454667038991197	26.141885039902412	27.98557732347653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	548.0
2	870.0
3	1289.0
4	1708.0
5	1365.5
6	1023.0
7	1135.0
8	1247.0
9	1307.0
10	1367.0
11	1381.0
12	1395.0
13	1396.5
14	1398.0
15	1410.0
16	1422.0
17	1540.5
18	1659.0
19	2018.5
20	2378.0
21	3236.5
22	4095.0
23	4279.0
24	4463.0
25	5026.0
26	7488.5
27	9388.0
28	10181.0
29	10974.0
30	13168.0
31	15362.0
32	16930.5
33	18499.0
34	23093.5
35	27688.0
36	29112.5
37	30537.0
38	33095.5
39	35654.0
40	43376.0
41	51098.0
42	56143.0
43	61188.0
44	67666.0
45	74144.0
46	82668.0
47	91192.0
48	100150.0
49	109108.0
50	102956.0
51	96804.0
52	83544.5
53	70285.0
54	64121.5
55	57958.0
56	53420.0
57	48882.0
58	47673.0
59	46464.0
60	43174.0
61	39884.0
62	35754.5
63	31625.0
64	27027.5
65	22430.0
66	19008.0
67	15586.0
68	12897.0
69	10208.0
70	8401.5
71	6595.0
72	5392.0
73	4189.0
74	3546.0
75	2284.0
76	1665.0
77	1272.0
78	879.0
79	599.0
80	319.0
81	241.5
82	164.0
83	110.0
84	56.0
85	37.5
86	19.0
87	22.5
88	26.0
89	13.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1020615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.213635989566832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8219188171887	16.422152543533603
2	12.42796166625782	6.018522797581146
3	5.291717533609976	3.84395166355321
4	2.933779902508677	2.8414991453140788
5	1.7925436497037663	2.17019997146683
6	1.2667701718240112	1.8403867093792583
7	0.9445069396176188	1.6008963057864563
8	0.7195033566430231	1.3937433896820512
9	0.5866120089977205	1.2783608687681378
>10	4.271483393742604	22.184184140660754
>50	1.660227777928161	28.658152972725254
>100	0.2764320124523816	9.591003468658705
>500	0.004498154048781353	0.7288505175586049
>1k	0.002044615476718797	1.4280955053318511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	3554	0.3482214155190743	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	3311	0.3244122416386198	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	3114	0.30511015417174936	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	2999	0.2938424381377895	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTG	1445	0.141581301470192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3200031353644616	0.0
2	0.0	0.0	0.0	1.3652552627582388	0.0
3	0.0	0.0	0.0	1.9400067606296203	0.0
4	0.0	0.0	0.0	2.589223164464563	0.0
5	0.0	0.0	0.0	3.7126634431200793	0.0
6	0.0	0.0	0.0	4.5460825090754104	0.0
7	0.0	0.0	0.0	5.2669223948305675	0.0
8	0.0	0.0	0.0	6.435237577343073	0.0
9	0.0	0.0	0.0	7.033014407979502	0.0
10	0.0	0.0	0.0	8.050930076473499	0.0
11	0.0	0.0	0.0	9.722569235216021	0.0
12	0.0	0.0	0.0	11.184334935308613	0.0
13	0.0	0.0	0.0	11.655129505249286	0.0
14	0.0	0.0	0.0	11.826888689662605	0.0
15	0.0	0.0	0.0	12.104858345213426	0.0
16	0.0	0.0	0.0	12.799635513881336	0.0
17	0.0	0.0	0.0	13.799816777139274	0.0
18	0.0	0.0	0.0	14.971365304252828	0.0
19	0.0	0.0	0.0	15.664084889992798	0.0
20	0.0	0.0	0.0	16.419021864268114	0.0
21	0.0	0.0	0.0	17.400488920895736	0.0
22	0.0	0.0	0.0	18.366573095633516	0.0
23	0.0	0.0	0.0	19.270047961278248	0.0
24	0.0	0.0	0.0	19.956496818094973	0.0
25	0.0	0.0	0.0	20.55319586719772	0.0
26	0.0	0.0	0.0	21.074548189082073	0.0
27	0.0	0.0	0.0	21.62294302944793	0.0
28	0.0	0.0	0.0	22.196028864949074	0.0
29	0.0	0.0	0.0	22.760492448180752	0.0
30	0.0	0.0	0.0	23.435085708126962	0.0
31	0.0	0.0	0.0	24.0568676729227	0.0
32	0.0	0.0	0.0	24.662482914713188	0.0
33	9.79801394257384E-5	0.0	0.0	25.265550672878607	0.0
34	9.79801394257384E-5	0.0	0.0	25.822469785374505	0.0
35	9.79801394257384E-5	0.0	0.0	26.412604165135726	0.0
36	9.79801394257384E-5	0.0	0.0	27.00802947242594	0.0
37	1.959602788514768E-4	0.0	0.0	27.6062962037595	0.0
38	1.959602788514768E-4	0.0	0.0	28.206424557742146	0.0
39	1.959602788514768E-4	0.0	0.0	28.815175163994258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	30	2.1652613E-6	45.000004	28
TATTACG	30	2.1652613E-6	45.000004	1
TCGTAAT	30	2.1652613E-6	45.000004	39
ACCCGTG	30	2.1652613E-6	45.000004	18
AGTCGAT	30	2.1652613E-6	45.000004	27
AACCGCA	20	7.032916E-4	45.0	10
TACCCGA	20	7.032916E-4	45.0	40
AAATCGT	25	3.8904982E-5	45.0	38
CGAACAG	20	7.032916E-4	45.0	33
AATTTCG	25	3.8904982E-5	45.0	39
CTCCGTA	20	7.032916E-4	45.0	22
CGTATCC	20	7.032916E-4	45.0	27
GTCGACA	20	7.032916E-4	45.0	30
CGACGTA	20	7.032916E-4	45.0	26
GCGGTAT	25	3.8904982E-5	45.0	39
GCGAAAT	20	7.032916E-4	45.0	41
GTTTACG	40	6.8139343E-9	45.0	1
CGTTGTA	20	7.032916E-4	45.0	31
GGCGTAA	25	3.8904982E-5	45.0	35
CGTCTTA	25	3.8904982E-5	45.0	13
>>END_MODULE