FastQCFastQC Report
Sat 14 Jan 2017
SRR2935318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC59490.310858248847541TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC56000.2926216496127466TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC52420.27391476558393174TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT46850.24480936222066388Illumina Paired End PCR Primer 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG25410.13277707351178378No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG23000.1201838918052352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG207.034462E-445.00000431
TTTACGG8050.043.043482
CGTATGG2400.042.1874962
TTACGGG13050.041.5517273
TACGGGT1800.041.254
TATGGGC11900.040.651264
TACGGGA7750.040.645164
CGATAAC900.040.016
TTTTACG5250.039.857141
CGAACGA850.039.70588322
ACATACG2550.039.70588317
GCCGATT3300.039.5454529
TTATGGG41150.039.2041323
TCTACGG5950.038.949582
CGTTTTT10300.038.8834951
GTACGGG4750.038.8421063
TTGGGCA21450.038.811195
CTACGGG8650.038.7572253
TTTGGGC44250.038.745764
TACGGGG13900.038.6870544
Produced by FastQC (version 0.11.5)