##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1913734
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31782421172431	33.0	31.0	34.0	30.0	34.0
2	32.47170505409843	34.0	31.0	34.0	31.0	34.0
3	32.53313051866142	34.0	31.0	34.0	30.0	34.0
4	35.96834408543716	37.0	35.0	37.0	35.0	37.0
5	31.738363325310623	37.0	35.0	37.0	0.0	37.0
6	33.4509095830455	37.0	35.0	37.0	17.0	37.0
7	35.205838951494826	37.0	35.0	37.0	32.0	37.0
8	35.757047217638394	37.0	35.0	37.0	35.0	37.0
9	37.787695677664715	39.0	38.0	39.0	35.0	39.0
10	37.5422111954953	39.0	37.0	39.0	35.0	39.0
11	37.42531198170697	39.0	37.0	39.0	35.0	39.0
12	37.063927379667184	39.0	35.0	39.0	34.0	39.0
13	36.890549574810294	39.0	35.0	39.0	33.0	39.0
14	37.68343928675563	40.0	37.0	41.0	33.0	41.0
15	37.89020104152406	40.0	37.0	41.0	33.0	41.0
16	38.09111454360951	40.0	37.0	41.0	33.0	41.0
17	38.16502084406715	40.0	37.0	41.0	33.0	41.0
18	38.16735293410683	40.0	37.0	41.0	34.0	41.0
19	38.17906929594186	40.0	37.0	41.0	34.0	41.0
20	38.06968157539136	40.0	36.0	41.0	33.0	41.0
21	38.02035601603985	40.0	36.0	41.0	33.0	41.0
22	38.06817143866389	40.0	36.0	41.0	34.0	41.0
23	38.05581862474095	40.0	36.0	41.0	34.0	41.0
24	38.00720476304439	40.0	36.0	41.0	34.0	41.0
25	37.91694770537598	40.0	36.0	41.0	34.0	41.0
26	37.8874974264971	40.0	36.0	41.0	34.0	41.0
27	37.83471161613892	40.0	35.0	41.0	34.0	41.0
28	37.806947569515934	40.0	35.0	41.0	34.0	41.0
29	37.7212851942851	40.0	35.0	41.0	33.0	41.0
30	37.66235223913041	40.0	35.0	41.0	33.0	41.0
31	37.41684581033728	40.0	35.0	41.0	33.0	41.0
32	37.45128790103536	39.0	35.0	41.0	33.0	41.0
33	37.47658556518304	39.0	35.0	41.0	33.0	41.0
34	37.39465359344611	39.0	35.0	41.0	33.0	41.0
35	37.30737239344653	39.0	35.0	41.0	32.0	41.0
36	37.30070375506732	39.0	35.0	41.0	32.0	41.0
37	37.26471390485825	39.0	35.0	41.0	32.0	41.0
38	37.22870733341206	39.0	35.0	41.0	32.0	41.0
39	37.221932619684864	39.0	35.0	41.0	33.0	41.0
40	37.117199673517845	39.0	35.0	41.0	32.0	41.0
41	36.998567198994216	39.0	35.0	41.0	32.0	41.0
42	37.02422593735597	39.0	35.0	41.0	32.0	41.0
43	37.006977458727285	39.0	35.0	41.0	32.0	41.0
44	36.98855483572952	39.0	35.0	41.0	32.0	41.0
45	36.98036456477232	39.0	35.0	41.0	32.0	41.0
46	36.9186036303896	39.0	35.0	41.0	32.0	41.0
47	36.85367297649517	39.0	35.0	41.0	32.0	41.0
48	36.79782874735987	38.0	35.0	41.0	32.0	41.0
49	36.77779409259594	38.0	35.0	40.0	32.0	41.0
50	36.69259364154057	38.0	35.0	40.0	32.0	41.0
51	36.0477689166833	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	14.0
10	22.0
11	19.0
12	26.0
13	29.0
14	37.0
15	67.0
16	85.0
17	208.0
18	309.0
19	562.0
20	905.0
21	1464.0
22	2290.0
23	3369.0
24	4873.0
25	7027.0
26	9641.0
27	12504.0
28	15702.0
29	20435.0
30	26822.0
31	36540.0
32	51519.0
33	82537.0
34	145461.0
35	153897.0
36	155595.0
37	242341.0
38	383581.0
39	555257.0
40	590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.54292707345953	4.880406576880591	54.947082509899495	16.629583839760382
2	19.518491075562224	4.739791423468465	53.910156792950325	21.831560708018984
3	21.183090230930734	4.372603507070471	51.28016746318976	23.16413879880903
4	19.35425717471707	5.167071285769078	50.07373020492921	25.40494133458464
5	15.965071425809437	17.40409064164612	43.66547284000807	22.965365092536373
6	21.361171406266493	6.484809278614478	52.85165022934222	19.30236908577681
7	82.72403583779145	2.932643721645746	9.068971967891045	5.27434847267175
8	84.97251969186941	3.0356883454022343	6.82712435479539	5.164667607932973
9	79.34070252187608	5.340919897958651	9.15607916251684	6.1622984176484294
10	42.58987926221722	23.272931347825768	17.714112828637628	16.423076561319387
11	32.71436887258104	23.448034052799397	23.646546489742043	20.191050584877523
12	29.657987996241904	20.25359846248225	29.299630983198295	20.78878255807756
13	27.502150246585995	21.355005450078224	31.04616420045837	20.09668010287741
14	20.869932811979094	23.52782570618487	34.57215057055996	21.03009091127607
15	19.1528185212783	22.37102962062648	37.08744266444553	21.388709193649692
16	25.096695779037216	21.97290741555514	30.322918441120866	22.60747836428678
17	25.55825417743532	22.14252346459853	27.882506137216563	24.416716220749592
18	26.38626893810739	21.054545720565134	30.13501353897668	22.4241718023508
19	26.34044229762339	23.988182265664925	27.63262814999368	22.038747286718007
20	24.37256170397767	28.055884464612113	28.02411411408273	19.547439717327485
21	24.206289902358428	24.930110454221953	30.69313708174699	20.170462561672625
22	23.11245972533278	21.93638196321955	31.23574122631463	23.715417085133044
23	21.796237094601445	23.190370239542172	30.955974027738442	24.05741863811794
24	22.524394717343164	25.82297226260285	28.575601415870754	23.077031604183233
25	24.27098018846924	24.295748520954323	25.937774006209853	25.495497284366586
26	21.06316760845551	24.492014041658873	26.165705369711777	28.27911298017384
27	20.712962198508254	23.283800151954242	29.65365092536371	26.34958672417379
28	18.908061412923637	23.745515311950356	28.454424700611476	28.89199857451454
29	20.834295675365542	25.440473963466186	26.982746818523367	26.7424835426449
30	24.03970457754317	23.342428989608795	28.321752134831694	24.296114298016338
31	24.216897437156888	24.259327576350735	26.940264425463518	24.583510561028856
32	25.422707649025412	23.95839756204363	26.27136268676838	24.347532102162578
33	25.207212705632028	23.064699691806698	27.430562450162878	24.2975251523984
34	20.923597532363434	22.71512132825147	30.48945151207012	25.87182962731498
35	20.60699135825564	25.487502442868237	28.13196609351143	25.773540105364695
36	23.90290395634921	24.232887120153585	27.15952164720907	24.704687276288137
37	22.070831160443404	26.820812087782315	27.25342184441516	23.85493490735912
38	21.932253907805368	26.15577713517134	27.798116143622885	24.1138528134004
39	21.88177667324717	24.03400890614892	28.960607900575525	25.123606520028385
40	24.36524616273735	21.831142677090966	30.119755410104016	23.68385575006767
41	21.532041548093936	21.846766583025644	28.67613785405913	27.945054014821284
42	20.022375105422174	21.77753021057263	31.414449448042415	26.785645235962786
43	20.364481166139075	22.00943286789073	31.111324771363208	26.514761194606983
44	22.038747286718007	21.959478171992554	28.627123727748998	27.37465081354044
45	20.715783907272378	21.72386549018829	27.186798165262253	30.373552437277073
46	21.22505008533056	21.734264009522743	29.833665493741556	27.207020411405136
47	18.619306549395056	24.12712529536498	31.7699324984559	25.483635656784067
48	19.292597612834385	24.84300325959616	30.327464527463064	25.536934600106388
49	22.43875063096543	21.91276321578652	31.002636730078475	24.645849423169572
50	20.882525993685643	22.1510930986229	28.98485369440058	27.981527213290875
51	19.84727240044855	23.149298700864385	27.725692285343733	29.277736613343336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	304.0
1	831.5
2	1359.0
3	2389.5
4	3420.0
5	2475.0
6	1530.0
7	1567.5
8	1605.0
9	1819.5
10	2034.0
11	2015.5
12	1997.0
13	2014.5
14	2032.0
15	2181.0
16	2330.0
17	2277.0
18	2224.0
19	2496.5
20	2769.0
21	3508.5
22	4248.0
23	5077.0
24	5906.0
25	7974.0
26	11616.0
27	13190.0
28	16906.0
29	20622.0
30	23625.5
31	26629.0
32	30874.0
33	35119.0
34	40591.0
35	46063.0
36	50029.5
37	53996.0
38	57620.5
39	61245.0
40	70684.5
41	80124.0
42	88183.5
43	96243.0
44	108633.0
45	121023.0
46	141891.5
47	162760.0
48	184270.0
49	205780.0
50	203512.0
51	201244.0
52	176809.0
53	152374.0
54	142287.5
55	132201.0
56	121704.0
57	111207.0
58	103551.5
59	95896.0
60	82521.5
61	69147.0
62	62425.5
63	55704.0
64	49720.0
65	43736.0
66	38143.0
67	32550.0
68	25868.5
69	19187.0
70	16556.0
71	13925.0
72	11694.5
73	9464.0
74	7526.0
75	4743.0
76	3898.0
77	2947.5
78	1997.0
79	1243.5
80	490.0
81	389.0
82	288.0
83	227.0
84	166.0
85	113.5
86	61.0
87	38.5
88	16.0
89	8.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1913734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.939556315259377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.9555550580592	17.446605049356954
2	12.198063038440713	6.084285601685522
3	5.129338664679504	3.837702914634354
4	2.6675153241493943	2.6610659459376476
5	1.5360481978496199	1.9154180266611636
6	1.0258532695901152	1.5350595232881385
7	0.7424567559243339	1.2961579453213798
8	0.5490573809729662	1.095459797846729
9	0.4159728805840564	0.9336761172852065
>10	3.9047594958236425	23.228377537266773
>50	1.6584427609360706	28.942840767589967
>100	0.20703547894980223	7.787600527208806
>500	0.006301078025847337	1.0236945309177592
>1k	0.0029255005120005493	1.2696842857978328
>5k	6.751155027693575E-4	0.9423714292017595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	5949	0.310858248847541	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	5600	0.2926216496127466	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	5242	0.27391476558393174	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	4685	0.24480936222066388	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2541	0.13277707351178378	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	2300	0.1201838918052352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0901546400910472E-4	0.0	0.0	0.2546330890290918	0.0
2	2.0901546400910472E-4	0.0	0.0	1.1954639464000745	0.0
3	2.0901546400910472E-4	0.0	0.0	1.7186819066808658	0.0
4	2.0901546400910472E-4	0.0	0.0	2.318922065449012	0.0
5	2.0901546400910472E-4	0.0	0.0	3.385057693493453	0.0
6	2.0901546400910472E-4	0.0	0.0	4.211034553391433	0.0
7	2.0901546400910472E-4	0.0	0.0	4.944260801135372	0.0
8	2.0901546400910472E-4	0.0	0.0	6.116106000102418	0.0
9	2.0901546400910472E-4	0.0	0.0	6.711800072528366	0.0
10	2.0901546400910472E-4	0.0	0.0	7.717582485340178	0.0
11	2.0901546400910472E-4	0.0	0.0	9.453142390739778	0.0
12	2.0901546400910472E-4	0.0	0.0	10.911809060193319	0.0
13	2.0901546400910472E-4	0.0	0.0	11.378279321995638	0.0
14	2.0901546400910472E-4	0.0	0.0	11.577680074660323	0.0
15	2.0901546400910472E-4	0.0	0.0	11.855304864730417	0.0
16	2.0901546400910472E-4	0.0	0.0	12.490032575060066	0.0
17	2.0901546400910472E-4	0.0	0.0	13.3539979955417	0.0
18	2.0901546400910472E-4	0.0	0.0	14.318813377407727	0.0
19	2.0901546400910472E-4	0.0	0.0	14.951868964025303	0.0
20	2.612693300113809E-4	0.0	0.0	15.660588148614176	0.0
21	2.612693300113809E-4	0.0	0.0	16.609936386143527	0.0
22	2.612693300113809E-4	0.0	0.0	17.584627748683985	0.0
23	3.135231960136571E-4	0.0	0.0	18.468449638246486	0.0
24	3.135231960136571E-4	0.0	0.0	19.17335429061719	0.0
25	3.135231960136571E-4	0.0	0.0	19.814195703269107	0.0
26	3.135231960136571E-4	0.0	0.0	20.38747286718008	0.0
27	3.6577706201593326E-4	0.0	0.0	21.094467674190874	0.0
28	4.1803092801820943E-4	0.0	0.0	21.72726199147844	0.0
29	4.1803092801820943E-4	0.0	0.0	22.448940134835876	0.0
30	4.1803092801820943E-4	0.0	0.0	23.20087326660863	0.0
31	4.1803092801820943E-4	0.0	0.0	23.806286558111	0.0
32	4.1803092801820943E-4	0.0	0.0	24.406944747807167	0.0
33	4.1803092801820943E-4	0.0	0.0	24.998458510952933	0.0
34	4.1803092801820943E-4	0.0	0.0	25.66981618135018	0.0
35	4.1803092801820943E-4	0.0	0.0	26.374250548926863	0.0
36	4.1803092801820943E-4	0.0	0.0	26.96654812006266	0.0
37	4.1803092801820943E-4	0.0	0.0	27.568512656408885	0.0
38	4.702847940204856E-4	0.0	0.0	28.365122843613584	0.0
39	4.702847940204856E-4	0.0	0.0	29.820602027240984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	20	7.034462E-4	45.000004	31
TTTACGG	805	0.0	43.04348	2
CGTATGG	240	0.0	42.187496	2
TTACGGG	1305	0.0	41.551727	3
TACGGGT	180	0.0	41.25	4
TATGGGC	1190	0.0	40.65126	4
TACGGGA	775	0.0	40.64516	4
CGATAAC	90	0.0	40.0	16
TTTTACG	525	0.0	39.85714	1
CGAACGA	85	0.0	39.705883	22
ACATACG	255	0.0	39.705883	17
GCCGATT	330	0.0	39.545452	9
TTATGGG	4115	0.0	39.204132	3
TCTACGG	595	0.0	38.94958	2
CGTTTTT	1030	0.0	38.883495	1
GTACGGG	475	0.0	38.842106	3
TTGGGCA	2145	0.0	38.81119	5
CTACGGG	865	0.0	38.757225	3
TTTGGGC	4425	0.0	38.74576	4
TACGGGG	1390	0.0	38.687054	4
>>END_MODULE