SRR2935317.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468737
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2641	0.5634289590964656	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2487	0.5305747146054184	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	2240	0.47787991986977774	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2056	0.43862549788047456	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	1894	0.4040645393898924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	1875	0.40001109364099696	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	1473	0.3142487151643672	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	960	0.20480567994419047	TruSeq Adapter, Index 20 (95% over 23bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	920	0.19627210994651584	No Hit
TTTTTTGGGGGTTTTGTTTTGTTTTGTTTTTTTCCACATTTTACATGACTG	858	0.18304507645012022	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	680	0.14507068996046824	No Hit
TTGTCGGGATGTGCGAGGGGAATAAATCCCTGTCTCTTATACACATCTGAC	628	0.13397704896349125	No Hit
CTTTGTGGGCAACGACGCGCAATCGGGAGAAACAAGCGAGATAGGAATGTC	614	0.13099029946430515	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	582	0.12416344346616547	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	544	0.11605655196837458	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	533	0.11370982021901407	TruSeq Adapter, Index 20 (95% over 23bp)
TGTCATGGGAGCTGGACGGTGGTGGCTCACGCCTTTAATCCCAGTTGGATT	532	0.11349648096907221	No Hit
TCCGCTGGGTGATGGGCTGCGAACATGGACTGAGCTTGTGGTGTGCTTTGC	487	0.10389621472168828	No Hit
GTTTTTGGGGTCTTTGGGATACCTCCAGTGCAACCACTTGGCTTATGCTAG	478	0.10197616147221149	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	476	0.10154948297232777	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	40	6.7993824E-9	45.000004	1
ATCTCGA	20	7.029006E-4	45.000004	6
AATCCAT	20	7.029006E-4	45.000004	36
ATCTCAT	20	7.029006E-4	45.000004	8
GCCTTAC	20	7.029006E-4	45.000004	27
AGTCCAT	20	7.029006E-4	45.000004	34
CGTATCC	20	7.029006E-4	45.000004	33
ATAGGTG	20	7.029006E-4	45.000004	24
GTCGACA	20	7.029006E-4	45.000004	14
TTTACGG	160	0.0	45.000004	2
GAATGCG	20	7.029006E-4	45.000004	34
CGACGGC	20	7.029006E-4	45.000004	31
CGACGCG	80	0.0	45.000004	13
CTATCGA	20	7.029006E-4	45.000004	41
ACTTGCG	20	7.029006E-4	45.000004	1
GAGTCGA	20	7.029006E-4	45.000004	28
CCCTAAG	20	7.029006E-4	45.000004	20
TTAGATG	20	7.029006E-4	45.000004	1
TAGGTGA	20	7.029006E-4	45.000004	34
CGGTTGC	20	7.029006E-4	45.000004	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content