##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468737
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.246406833682855	33.0	31.0	34.0	30.0	34.0
2	32.40021589932094	34.0	31.0	34.0	30.0	34.0
3	32.47917275572443	34.0	31.0	34.0	30.0	34.0
4	35.9321111838835	37.0	35.0	37.0	35.0	37.0
5	31.564578857653654	37.0	35.0	37.0	0.0	37.0
6	33.305593541794224	37.0	35.0	37.0	17.0	37.0
7	35.19512434478183	37.0	35.0	37.0	32.0	37.0
8	35.709532211026655	37.0	35.0	37.0	35.0	37.0
9	37.748573293766015	39.0	38.0	39.0	35.0	39.0
10	37.50651644738947	39.0	37.0	39.0	35.0	39.0
11	37.396559264576936	39.0	37.0	39.0	35.0	39.0
12	37.01778609326765	39.0	35.0	39.0	34.0	39.0
13	36.784712962706166	39.0	35.0	39.0	33.0	39.0
14	37.89810917422776	40.0	36.0	41.0	33.0	41.0
15	38.057569596596814	40.0	36.0	41.0	33.0	41.0
16	38.181914378425425	40.0	36.0	41.0	34.0	41.0
17	38.18260773098774	40.0	36.0	41.0	34.0	41.0
18	38.193746173227204	40.0	36.0	41.0	34.0	41.0
19	38.21231735493464	40.0	36.0	41.0	34.0	41.0
20	38.08528876534176	40.0	36.0	41.0	34.0	41.0
21	38.03037310901422	40.0	36.0	41.0	34.0	41.0
22	37.98696283843605	40.0	36.0	41.0	34.0	41.0
23	37.92026232194173	40.0	36.0	41.0	34.0	41.0
24	37.90795904739758	40.0	35.0	41.0	34.0	41.0
25	37.84459088998735	40.0	35.0	41.0	34.0	41.0
26	37.803132673546145	40.0	35.0	41.0	34.0	41.0
27	37.69816336239725	40.0	35.0	41.0	33.0	41.0
28	37.71244429178836	39.0	36.0	41.0	34.0	41.0
29	37.67067673343474	39.0	36.0	41.0	33.0	41.0
30	37.625683485622	39.0	36.0	41.0	33.0	41.0
31	37.446704655275774	39.0	35.0	41.0	33.0	41.0
32	37.447848153655464	39.0	35.0	41.0	33.0	41.0
33	37.44396964609152	39.0	35.0	41.0	33.0	41.0
34	37.481114142898896	39.0	35.0	41.0	33.0	41.0
35	37.38526295129252	39.0	35.0	41.0	33.0	41.0
36	37.351292942524275	39.0	35.0	41.0	33.0	41.0
37	37.29151955147556	39.0	35.0	41.0	33.0	41.0
38	37.29709410607654	39.0	35.0	41.0	33.0	41.0
39	37.31767494351843	39.0	35.0	41.0	33.0	41.0
40	37.26324996746576	39.0	35.0	41.0	33.0	41.0
41	37.211203724049945	39.0	35.0	41.0	33.0	41.0
42	37.21558357885125	39.0	35.0	41.0	33.0	41.0
43	37.13624271179788	39.0	35.0	41.0	33.0	41.0
44	37.09445595291176	39.0	35.0	41.0	33.0	41.0
45	37.07680212997907	39.0	35.0	41.0	33.0	41.0
46	36.97787671978103	39.0	35.0	41.0	33.0	41.0
47	36.93758973582201	39.0	35.0	41.0	33.0	41.0
48	36.94465553178008	39.0	35.0	41.0	33.0	41.0
49	36.97342006284974	39.0	35.0	41.0	33.0	41.0
50	36.872100986267355	39.0	35.0	41.0	33.0	41.0
51	36.33442634142387	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	5.0
12	6.0
13	7.0
14	5.0
15	9.0
16	15.0
17	32.0
18	46.0
19	113.0
20	254.0
21	423.0
22	642.0
23	912.0
24	1292.0
25	1727.0
26	2225.0
27	2632.0
28	3271.0
29	4396.0
30	5863.0
31	8177.0
32	11577.0
33	18090.0
34	31769.0
35	52206.0
36	38379.0
37	57011.0
38	92911.0
39	134564.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.38011720858392	4.559699789007482	55.33529463217113	14.724888370237469
2	18.324561534506557	6.827282676639565	52.1435261137909	22.704629675062986
3	20.64142578887521	7.38260474423824	50.05408149986026	21.921887967026287
4	17.974685164601897	5.1122484463569124	52.39419973247258	24.51886665656861
5	16.3134124253046	17.081007046595424	43.057834137266745	23.54774639083324
6	19.93932631731653	8.363751954720877	53.354226357210976	18.342695370751617
7	81.38465706782269	2.042083300443532	11.177483322204136	5.395776309529651
8	81.50178031604077	4.4519634677868405	7.769602143632784	6.276654072539611
9	75.56070888365971	5.9071504916403015	10.077292810253939	8.454847814446055
10	39.4436539040016	27.170886872595933	17.438136950998107	15.947322272404355
11	33.97619560649149	23.390728702876025	25.3937709205802	17.23930477005229
12	29.60935449943145	20.469261014172126	28.66447496143893	21.256909524957493
13	22.890448161762354	29.87026840211035	29.933843498593028	17.305439937534267
14	16.60910062572402	32.46212694965407	28.88272101412946	22.046051410492453
15	14.63592590301171	24.480465591579073	40.93766867134448	19.94593983406473
16	16.23149015332692	24.295500461879477	35.31916618487553	24.153843199918075
17	16.64024815621553	23.07754668396137	29.385348286992492	30.896856872830607
18	19.61185056865577	21.99463665125646	34.21662894117597	24.1768838389118
19	22.928635887501947	25.416598220323976	28.947576146111786	22.70718974606229
20	24.728579139261463	25.612230312520666	29.533619065702087	20.12557148251578
21	22.547398647855836	30.04136648056373	26.559029903762664	20.852204967817777
22	20.013141697796417	26.29171582358551	27.05312360662802	26.642018871990047
23	18.789427760129882	27.637673151468732	27.548070666493153	26.024828421908236
24	20.363658085450904	25.00506680718612	30.624635989904785	24.00663911745819
25	17.999219178345214	28.87418744413178	27.116058685360873	26.01053469216213
26	16.66158208120972	30.005738825823435	27.124805594608492	26.207873498358353
27	19.26304089500082	29.988031668078264	28.256783654800028	22.49214378212089
28	17.699050853677008	25.847543505206545	32.71344058608558	23.739965055030858
29	17.172529584820488	24.457851631085237	30.832641758598108	27.536977025496174
30	19.328322705483032	27.987762860623334	27.627859545971408	25.056054887922226
31	23.923436809980863	27.120538809609652	24.542120634812274	24.413903745597214
32	21.218081781468072	27.648340113965826	25.659378286757818	25.47419981780828
33	20.27213554722584	27.29846374406117	25.920292189436722	26.50910851927627
34	18.361895903246385	24.629376388038494	28.87248073013225	28.136246978582875
35	16.70040982469914	25.649778020510432	28.730396789670966	28.919415365119463
36	21.305977552444123	26.034002009655733	28.210915716062523	24.44910472183762
37	19.145490968282854	27.9427482788856	30.078487510053613	22.83327324277793
38	18.96799271233122	27.591591873481292	27.381879390788438	26.05853602339905
39	18.239225834529808	25.672178641754336	30.747092719371416	25.34150280434444
40	21.82161851955361	22.617160582586823	31.00416651555136	24.557054382308202
41	16.318745906553143	23.5409195348351	31.5934095238908	28.54692503472096
42	19.81281614210101	22.610760405088566	31.12854329826747	26.44788015454295
43	21.500542948391104	24.560681149557215	28.224569428058803	25.714206473992878
44	20.478007923419742	24.92826467720705	27.829678476416415	26.764048922956796
45	18.85214949961279	26.949227391906334	25.04325453292571	29.155368575555162
46	21.208268175970744	26.24158109984917	27.896667000898155	24.653483723281926
47	17.132421805831417	24.44611797233843	32.63727847385634	25.78418174797381
48	19.095569583796458	22.39614111964705	32.85424449104722	25.654044805509272
49	20.85625841356667	19.759908008115428	33.12411864222368	26.259714936094227
50	19.667105434390713	22.348566466910018	30.58858165666461	27.395746442034657
51	17.630995632945552	22.82004620928154	28.025523907863043	31.523434249909865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	217.0
2	299.0
3	483.0
4	667.0
5	546.5
6	426.0
7	476.0
8	526.0
9	830.5
10	1135.0
11	1136.0
12	1137.0
13	1214.0
14	1291.0
15	1266.5
16	1242.0
17	1074.5
18	907.0
19	999.5
20	1092.0
21	1235.0
22	1378.0
23	1519.0
24	1660.0
25	1844.5
26	3375.0
27	4721.0
28	5744.0
29	6767.0
30	6345.5
31	5924.0
32	7411.5
33	8899.0
34	10093.0
35	11287.0
36	11448.5
37	11610.0
38	12939.0
39	14268.0
40	19420.0
41	24572.0
42	28022.0
43	31472.0
44	35771.5
45	40071.0
46	49997.0
47	59923.0
48	61593.0
49	63263.0
50	58586.5
51	53910.0
52	44520.5
53	35131.0
54	29115.5
55	23100.0
56	19845.5
57	16591.0
58	14686.0
59	12781.0
60	11029.0
61	9277.0
62	8280.5
63	7284.0
64	6009.5
65	4735.0
66	3799.5
67	2864.0
68	2741.0
69	2618.0
70	1959.0
71	1300.0
72	1241.5
73	1183.0
74	828.5
75	494.0
76	514.0
77	339.5
78	165.0
79	110.0
80	55.0
81	49.5
82	44.0
83	27.0
84	10.0
85	5.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.64949373818093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7297931196674	16.695051113915316
2	12.482416486551463	6.153704940452334
3	5.559965755730305	4.111510232411236
4	3.137669162251603	3.0936782546961714
5	1.9091350824079658	2.3529606629578343
6	1.2922394119725849	1.91118283761893
7	0.9304509442931085	1.6054601307526255
8	0.6834859059835242	1.347806524774064
9	0.5878319873544077	1.304078480225543
>10	3.971343197629328	20.12490086698132
>50	1.356535000510351	24.25555330218314
>100	0.3443511924372429	12.432040115646044
>500	0.00869573718275866	1.4684811522808805
>1k	0.006087016027931062	3.1435913851045676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2641	0.5634289590964656	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2487	0.5305747146054184	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	2240	0.47787991986977774	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2056	0.43862549788047456	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	1894	0.4040645393898924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	1875	0.40001109364099696	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	1473	0.3142487151643672	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	960	0.20480567994419047	TruSeq Adapter, Index 20 (95% over 23bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	920	0.19627210994651584	No Hit
TTTTTTGGGGGTTTTGTTTTGTTTTGTTTTTTTCCACATTTTACATGACTG	858	0.18304507645012022	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	680	0.14507068996046824	No Hit
TTGTCGGGATGTGCGAGGGGAATAAATCCCTGTCTCTTATACACATCTGAC	628	0.13397704896349125	No Hit
CTTTGTGGGCAACGACGCGCAATCGGGAGAAACAAGCGAGATAGGAATGTC	614	0.13099029946430515	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	582	0.12416344346616547	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	544	0.11605655196837458	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	533	0.11370982021901407	TruSeq Adapter, Index 20 (95% over 23bp)
TGTCATGGGAGCTGGACGGTGGTGGCTCACGCCTTTAATCCCAGTTGGATT	532	0.11349648096907221	No Hit
TCCGCTGGGTGATGGGCTGCGAACATGGACTGAGCTTGTGGTGTGCTTTGC	487	0.10389621472168828	No Hit
GTTTTTGGGGTCTTTGGGATACCTCCAGTGCAACCACTTGGCTTATGCTAG	478	0.10197616147221149	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	476	0.10154948297232777	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4962270953647781	0.0
2	0.0	0.0	0.0	2.126992321920395	0.0
3	0.0	0.0	0.0	2.9449350061975053	0.0
4	0.0	0.0	0.0	3.9512562481732827	0.0
5	0.0	0.0	0.0	5.7721067464271005	0.0
6	0.0	0.0	0.0	6.831549461638403	0.0
7	0.0	0.0	0.0	7.867311520106157	0.0
8	0.0	0.0	0.0	9.625440278877068	0.0
9	0.0	0.0	0.0	10.460236763899585	0.0
10	0.0	0.0	0.0	12.341675609136894	0.0
11	0.0	0.0	0.0	14.61757872751671	0.0
12	0.0	0.0	0.0	17.067353334599147	0.0
13	0.0	0.0	0.0	17.704170995675614	0.0
14	2.1333924994186505E-4	0.0	0.0	17.934364046362887	0.0
15	2.1333924994186505E-4	0.0	0.0	18.380243078741383	0.0
16	2.1333924994186505E-4	0.0	0.0	19.312962279487216	0.0
17	2.1333924994186505E-4	0.0	0.0	20.553743357149106	0.0
18	2.1333924994186505E-4	0.0	0.0	22.189628725703326	0.0
19	2.1333924994186505E-4	0.0	0.0	23.123841301198752	0.0
20	2.1333924994186505E-4	0.0	0.0	24.09133479968511	0.0
21	4.266784998837301E-4	0.0	0.0	25.197925489133564	0.0
22	4.266784998837301E-4	0.0	0.0	26.175872610867074	0.0
23	4.266784998837301E-4	0.0	0.0	27.171100211845875	0.0
24	4.266784998837301E-4	0.0	0.0	27.90754730264519	0.0
25	4.266784998837301E-4	0.0	0.0	28.602179900455905	0.0
26	4.266784998837301E-4	0.0	0.0	29.22278377853679	0.0
27	4.266784998837301E-4	0.0	0.0	29.76957227613779	0.0
28	4.266784998837301E-4	0.0	0.0	30.34153480523193	0.0
29	4.266784998837301E-4	0.0	0.0	31.01526015654834	0.0
30	4.266784998837301E-4	0.0	0.0	31.895497901808476	0.0
31	4.266784998837301E-4	0.0	0.0	32.55812961212791	0.0
32	4.266784998837301E-4	0.0	0.0	33.104064752729144	0.0
33	4.266784998837301E-4	0.0	0.0	33.649146536330605	0.0
34	4.266784998837301E-4	0.0	0.0	34.21812231592556	0.0
35	4.266784998837301E-4	0.0	0.0	34.8267791960097	0.0
36	4.266784998837301E-4	0.0	0.0	35.35052705461698	0.0
37	4.266784998837301E-4	0.0	0.0	35.937636670456996	0.0
38	4.266784998837301E-4	0.0	0.0	36.47845166905962	0.0
39	4.266784998837301E-4	0.0	0.0	37.064921267149806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	40	6.7993824E-9	45.000004	1
ATCTCGA	20	7.029006E-4	45.000004	6
AATCCAT	20	7.029006E-4	45.000004	36
ATCTCAT	20	7.029006E-4	45.000004	8
GCCTTAC	20	7.029006E-4	45.000004	27
AGTCCAT	20	7.029006E-4	45.000004	34
CGTATCC	20	7.029006E-4	45.000004	33
ATAGGTG	20	7.029006E-4	45.000004	24
GTCGACA	20	7.029006E-4	45.000004	14
TTTACGG	160	0.0	45.000004	2
GAATGCG	20	7.029006E-4	45.000004	34
CGACGGC	20	7.029006E-4	45.000004	31
CGACGCG	80	0.0	45.000004	13
CTATCGA	20	7.029006E-4	45.000004	41
ACTTGCG	20	7.029006E-4	45.000004	1
GAGTCGA	20	7.029006E-4	45.000004	28
CCCTAAG	20	7.029006E-4	45.000004	20
TTAGATG	20	7.029006E-4	45.000004	1
TAGGTGA	20	7.029006E-4	45.000004	34
CGGTTGC	20	7.029006E-4	45.000004	29
>>END_MODULE