SRR2935316.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827450
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	5594	0.6760529337120067	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	5309	0.6416097649404798	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	4901	0.5923016496465043	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	4495	0.5432352408000484	TruSeq Adapter, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	2044	0.24702398936491632	No Hit
GCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	1230	0.14864946522448488	TruSeq Adapter, Index 16 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	1124	0.135839023505952	No Hit
TGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCTT	1108	0.1339053719257961	TruSeq Adapter, Index 16 (95% over 24bp)
TTGTTGGGGATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGT	1086	0.13124660100308175	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	921	0.11130581908272404	No Hit
TTGTTCGGGAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC	857	0.10357121276210043	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	856	0.10345035953834068	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	851	0.10284609341954197	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.032139E-4	45.000004	20
CGAGTTA	20	7.032139E-4	45.000004	33
GATCGGC	20	7.032139E-4	45.000004	19
CCGATTA	20	7.032139E-4	45.000004	10
CACGGGT	45	3.8380676E-10	45.000004	4
GACGTAT	20	7.032139E-4	45.000004	15
AAGACTC	20	7.032139E-4	45.000004	18
CGACCGA	20	7.032139E-4	45.000004	17
TAAGTCG	20	7.032139E-4	45.000004	19
TGACGTT	20	7.032139E-4	45.000004	31
TGACGTA	20	7.032139E-4	45.000004	14
ACGGGTG	45	3.8380676E-10	45.000004	5
TGCATCA	20	7.032139E-4	45.000004	16
ATTCCGA	40	6.8102963E-9	45.000004	12
TACGCGG	45	3.8380676E-10	45.000004	2
TCTACGT	20	7.032139E-4	45.000004	44
CTAGTGA	20	7.032139E-4	45.000004	10
GTTCGCA	20	7.032139E-4	45.000004	9
CGGATCG	20	7.032139E-4	45.000004	17
AGCATCG	20	7.032139E-4	45.000004	40